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Q41594

- TASY_TAXBR

UniProt

Q41594 - TASY_TAXBR

Protein

Taxadiene synthase

Gene

TDC1

Organism
Taxus brevifolia (Pacific yew)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the cyclization of the ubiquitous isoprenoid intermediate geranylgeranyl diphosphate to taxa-4,11-diene, the parent olefin with a taxane skeleton.

    Catalytic activityi

    Geranylgeranyl diphosphate = taxa-4,11-diene + diphosphate.

    Cofactori

    Binds 3 magnesium ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi613 – 6131Magnesium 1By similarity
    Metal bindingi613 – 6131Magnesium 2By similarity
    Metal bindingi617 – 6171Magnesium 1By similarity
    Metal bindingi617 – 6171Magnesium 2By similarity
    Metal bindingi757 – 7571Magnesium 3By similarity
    Metal bindingi761 – 7611Magnesium 3By similarity
    Metal bindingi765 – 7651Magnesium 3By similarity

    GO - Molecular functioni

    1. magnesium ion binding Source: InterPro
    2. taxadiene synthase activity Source: UniProtKB-EC
    3. terpene synthase activity Source: InterPro

    GO - Biological processi

    1. paclitaxel biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Taxol biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13385.
    UniPathwayiUPA00842; UER00806.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Taxadiene synthase (EC:4.2.3.17)
    Alternative name(s):
    Taxa-4(5),11(12)-diene synthase
    Gene namesi
    Name:TDC1
    OrganismiTaxus brevifolia (Pacific yew)
    Taxonomic identifieri46220 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesTaxaceaeTaxus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 862862Taxadiene synthasePRO_0000186450Add
    BLAST

    Structurei

    Secondary structure

    1
    862
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi112 – 12817
    Helixi139 – 1479
    Beta strandi149 – 1513
    Beta strandi157 – 1604
    Helixi162 – 1709
    Beta strandi174 – 1763
    Helixi186 – 20217
    Helixi207 – 22115
    Beta strandi226 – 2283
    Helixi234 – 24714
    Helixi257 – 27418
    Helixi282 – 2909
    Helixi292 – 2943
    Helixi299 – 3035
    Helixi314 – 32411
    Helixi327 – 34014
    Helixi351 – 36515
    Helixi368 – 3714
    Helixi372 – 38413
    Helixi402 – 41413
    Helixi421 – 4277
    Helixi445 – 45511
    Helixi463 – 48220
    Beta strandi486 – 4883
    Helixi489 – 49911
    Helixi502 – 5043
    Helixi507 – 51711
    Beta strandi524 – 5307
    Turni533 – 5353
    Helixi538 – 56831
    Turni571 – 5755
    Helixi578 – 58710
    Helixi593 – 5953
    Helixi596 – 61823
    Helixi622 – 63413
    Helixi640 – 6423
    Helixi645 – 66925
    Helixi674 – 69623
    Helixi703 – 71311
    Helixi716 – 72510
    Beta strandi727 – 7293
    Helixi733 – 7353
    Helixi736 – 7394
    Helixi744 – 76623
    Turni767 – 7693
    Helixi774 – 7818
    Helixi787 – 81024
    Beta strandi814 – 8163
    Helixi818 – 8269
    Helixi828 – 8347
    Beta strandi840 – 8423
    Beta strandi844 – 8463
    Helixi848 – 8569

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3P5PX-ray1.82A108-862[»]
    3P5RX-ray2.25A/B108-862[»]
    ProteinModelPortaliQ41594.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ41594.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi613 – 6175DDXXD motif

    Domaini

    The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

    Sequence similaritiesi

    Belongs to the terpene synthase family.Curated

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    1.50.10.20. 1 hit.
    1.50.30.10. 1 hit.
    InterProiIPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 2 hits.
    SSF48576. SSF48576. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q41594-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAQLSFNAAL KMNALGNKAI HDPTNCRAKS ERQMMWVCSR SGRTRVKMSR    50
    GSGGPGPVVM MSSSTGTSKV VSETSSTIVD DIPRLSANYH GDLWHHNVIQ 100
    TLETPFRESS TYQERADELV VKIKDMFNAL GDGDISPSAY DTAWVARLAT 150
    ISSDGSEKPR FPQALNWVFN NQLQDGSWGI ESHFSLCDRL LNTTNSVIAL 200
    SVWKTGHSQV QQGAEFIAEN LRLLNEEDEL SPDFQIIFPA LLQKAKALGI 250
    NLPYDLPFIK YLSTTREARL TDVSAAADNI PANMLNALEG LEEVIDWNKI 300
    MRFQSKDGSF LSSPASTACV LMNTGDEKCF TFLNNLLDKF GGCVPCMYSI 350
    DLLERLSLVD NIEHLGIGRH FKQEIKGALD YVYRHWSERG IGWGRDSLVP 400
    DLNTTALGLR TLRMHGYNVS SDVLNNFKDE NGRFFSSAGQ THVELRSVVN 450
    LFRASDLAFP DERAMDDARK FAEPYLREAL ATKISTNTKL FKEIEYVVEY 500
    PWHMSIPRLE ARSYIDSYDD NYVWQRKTLY RMPSLSNSKC LELAKLDFNI 550
    VQSLHQEELK LLTRWWKESG MADINFTRHR VAEVYFSSAT FEPEYSATRI 600
    AFTKIGCLQV LFDDMADIFA TLDELKSFTE GVKRWDTSLL HEIPECMQTC 650
    FKVWFKLMEE VNNDVVKVQG RDMLAHIRKP WELYFNCYVQ EREWLEAGYI 700
    PTFEEYLKTY AISVGLGPCT LQPILLMGEL VKDDVVEKVH YPSNMFELVS 750
    LSWRLTNDTK TYQAEKARGQ QASGIACYMK DNPGATEEDA IKHICRVVDR 800
    ALKEASFEYF KPSNDIPMGC KSFIFNLRLC VQIFYKFIDG YGIANEEIKD 850
    YIRKVYIDPI QV 862
    Length:862
    Mass (Da):98,304
    Last modified:November 1, 1996 - v1
    Checksum:i9141B59780CD79A1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti148 – 1481L → V in AAK83566. (PubMed:11404343)Curated
    Sequence conflicti767 – 7671A → V in AAK83566. (PubMed:11404343)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48796 mRNA. Translation: AAC49310.1.
    AF326519 Genomic DNA. Translation: AAK83566.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U48796 mRNA. Translation: AAC49310.1 .
    AF326519 Genomic DNA. Translation: AAK83566.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3P5P X-ray 1.82 A 108-862 [» ]
    3P5R X-ray 2.25 A/B 108-862 [» ]
    ProteinModelPortali Q41594.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00842 ; UER00806 .
    BioCyci MetaCyc:MONOMER-13385.

    Miscellaneous databases

    EvolutionaryTracei Q41594.

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    1.50.10.20. 1 hit.
    1.50.30.10. 1 hit.
    InterProi IPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48239. SSF48239. 2 hits.
    SSF48576. SSF48576. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A cDNA clone for taxadiene synthase, the diterpene cyclase that catalyzes the committed step of taxol biosynthesis."
      Wildung M.R., Croteau R.B.
      J. Biol. Chem. 271:9201-9204(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Genomic organization of plant terpene synthases and molecular evolutionary implications."
      Trapp S.C., Croteau R.B.
      Genetics 158:811-832(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.

    Entry informationi

    Entry nameiTASY_TAXBR
    AccessioniPrimary (citable) accession number: Q41594
    Secondary accession number(s): Q94FV8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 22, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3