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Q41594

- TASY_TAXBR

UniProt

Q41594 - TASY_TAXBR

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Protein

Taxadiene synthase

Gene

TDC1

Organism
Taxus brevifolia (Pacific yew)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the cyclization of the ubiquitous isoprenoid intermediate geranylgeranyl diphosphate to taxa-4,11-diene, the parent olefin with a taxane skeleton.

Catalytic activityi

Geranylgeranyl diphosphate = taxa-4,11-diene + diphosphate.

Cofactori

Binds 3 magnesium ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi613 – 6131Magnesium 1By similarity
Metal bindingi613 – 6131Magnesium 2By similarity
Metal bindingi617 – 6171Magnesium 1By similarity
Metal bindingi617 – 6171Magnesium 2By similarity
Metal bindingi757 – 7571Magnesium 3By similarity
Metal bindingi761 – 7611Magnesium 3By similarity
Metal bindingi765 – 7651Magnesium 3By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. taxadiene synthase activity Source: UniProtKB-EC
  3. terpene synthase activity Source: InterPro

GO - Biological processi

  1. paclitaxel biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Taxol biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13385.
UniPathwayiUPA00842; UER00806.

Names & Taxonomyi

Protein namesi
Recommended name:
Taxadiene synthase (EC:4.2.3.17)
Alternative name(s):
Taxa-4(5),11(12)-diene synthase
Gene namesi
Name:TDC1
OrganismiTaxus brevifolia (Pacific yew)
Taxonomic identifieri46220 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesTaxaceaeTaxus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 862862Taxadiene synthasePRO_0000186450Add
BLAST

Structurei

Secondary structure

1
862
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi112 – 12817
Helixi139 – 1479
Beta strandi149 – 1513
Beta strandi157 – 1604
Helixi162 – 1709
Beta strandi174 – 1763
Helixi186 – 20217
Helixi207 – 22115
Beta strandi226 – 2283
Helixi234 – 24714
Helixi257 – 27418
Helixi282 – 2909
Helixi292 – 2943
Helixi299 – 3035
Helixi314 – 32411
Helixi327 – 34014
Helixi351 – 36515
Helixi368 – 3714
Helixi372 – 38413
Helixi402 – 41413
Helixi421 – 4277
Helixi445 – 45511
Helixi463 – 48220
Beta strandi486 – 4883
Helixi489 – 49911
Helixi502 – 5043
Helixi507 – 51711
Beta strandi524 – 5307
Turni533 – 5353
Helixi538 – 56831
Turni571 – 5755
Helixi578 – 58710
Helixi593 – 5953
Helixi596 – 61823
Helixi622 – 63413
Helixi640 – 6423
Helixi645 – 66925
Helixi674 – 69623
Helixi703 – 71311
Helixi716 – 72510
Beta strandi727 – 7293
Helixi733 – 7353
Helixi736 – 7394
Helixi744 – 76623
Turni767 – 7693
Helixi774 – 7818
Helixi787 – 81024
Beta strandi814 – 8163
Helixi818 – 8269
Helixi828 – 8347
Beta strandi840 – 8423
Beta strandi844 – 8463
Helixi848 – 8569

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3P5PX-ray1.82A108-862[»]
3P5RX-ray2.25A/B108-862[»]
ProteinModelPortaliQ41594.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ41594.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi613 – 6175DDXXD motif

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similaritiesi

Belongs to the terpene synthase family.Curated

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Q41594-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQLSFNAAL KMNALGNKAI HDPTNCRAKS ERQMMWVCSR SGRTRVKMSR
60 70 80 90 100
GSGGPGPVVM MSSSTGTSKV VSETSSTIVD DIPRLSANYH GDLWHHNVIQ
110 120 130 140 150
TLETPFRESS TYQERADELV VKIKDMFNAL GDGDISPSAY DTAWVARLAT
160 170 180 190 200
ISSDGSEKPR FPQALNWVFN NQLQDGSWGI ESHFSLCDRL LNTTNSVIAL
210 220 230 240 250
SVWKTGHSQV QQGAEFIAEN LRLLNEEDEL SPDFQIIFPA LLQKAKALGI
260 270 280 290 300
NLPYDLPFIK YLSTTREARL TDVSAAADNI PANMLNALEG LEEVIDWNKI
310 320 330 340 350
MRFQSKDGSF LSSPASTACV LMNTGDEKCF TFLNNLLDKF GGCVPCMYSI
360 370 380 390 400
DLLERLSLVD NIEHLGIGRH FKQEIKGALD YVYRHWSERG IGWGRDSLVP
410 420 430 440 450
DLNTTALGLR TLRMHGYNVS SDVLNNFKDE NGRFFSSAGQ THVELRSVVN
460 470 480 490 500
LFRASDLAFP DERAMDDARK FAEPYLREAL ATKISTNTKL FKEIEYVVEY
510 520 530 540 550
PWHMSIPRLE ARSYIDSYDD NYVWQRKTLY RMPSLSNSKC LELAKLDFNI
560 570 580 590 600
VQSLHQEELK LLTRWWKESG MADINFTRHR VAEVYFSSAT FEPEYSATRI
610 620 630 640 650
AFTKIGCLQV LFDDMADIFA TLDELKSFTE GVKRWDTSLL HEIPECMQTC
660 670 680 690 700
FKVWFKLMEE VNNDVVKVQG RDMLAHIRKP WELYFNCYVQ EREWLEAGYI
710 720 730 740 750
PTFEEYLKTY AISVGLGPCT LQPILLMGEL VKDDVVEKVH YPSNMFELVS
760 770 780 790 800
LSWRLTNDTK TYQAEKARGQ QASGIACYMK DNPGATEEDA IKHICRVVDR
810 820 830 840 850
ALKEASFEYF KPSNDIPMGC KSFIFNLRLC VQIFYKFIDG YGIANEEIKD
860
YIRKVYIDPI QV
Length:862
Mass (Da):98,304
Last modified:November 1, 1996 - v1
Checksum:i9141B59780CD79A1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti148 – 1481L → V in AAK83566. (PubMed:11404343)Curated
Sequence conflicti767 – 7671A → V in AAK83566. (PubMed:11404343)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U48796 mRNA. Translation: AAC49310.1.
AF326519 Genomic DNA. Translation: AAK83566.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U48796 mRNA. Translation: AAC49310.1 .
AF326519 Genomic DNA. Translation: AAK83566.1 .

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3P5P X-ray 1.82 A 108-862 [» ]
3P5R X-ray 2.25 A/B 108-862 [» ]
ProteinModelPortali Q41594.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00842 ; UER00806 .
BioCyci MetaCyc:MONOMER-13385.

Miscellaneous databases

EvolutionaryTracei Q41594.

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProi IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 2 hits.
SSF48576. SSF48576. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "A cDNA clone for taxadiene synthase, the diterpene cyclase that catalyzes the committed step of taxol biosynthesis."
    Wildung M.R., Croteau R.B.
    J. Biol. Chem. 271:9201-9204(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Genomic organization of plant terpene synthases and molecular evolutionary implications."
    Trapp S.C., Croteau R.B.
    Genetics 158:811-832(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.

Entry informationi

Entry nameiTASY_TAXBR
AccessioniPrimary (citable) accession number: Q41594
Secondary accession number(s): Q94FV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: November 1, 1996
Last modified: October 1, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3