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Q41594 (TASY_TAXBR) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Taxadiene synthase
EC=4.2.3.17
Alternative name(s):
Taxa-4(5),11(12)-diene synthase
Gene names
Name:TDC1
OrganismTaxus brevifolia (Pacific yew)
Taxonomic identifier46220 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Coniferopsida › Coniferales › Taxaceae › Taxus

Protein attributes

Sequence length862 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the cyclization of the ubiquitous isoprenoid intermediate geranylgeranyl diphosphate to taxa-4,11-diene, the parent olefin with a taxane skeleton.

Catalytic activity

Geranylgeranyl diphosphate = taxa-4,11-diene + diphosphate.

Cofactor

Binds 3 magnesium ions per subunit By similarity.

Pathway

Alkaloid biosynthesis; taxol biosynthesis; taxa-4(20),11-dien-5alpha-ol from geranylgeranyl diphosphate: step 1/2.

Domain

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similarities

Belongs to the terpene synthase family.

Ontologies

Keywords
   Biological processTaxol biosynthesis
   LigandMagnesium
Metal-binding
   Molecular functionLyase
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processpaclitaxel biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionmagnesium ion binding

Inferred from electronic annotation. Source: InterPro

taxadiene synthase activity

Inferred from electronic annotation. Source: EC

terpene synthase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 862862Taxadiene synthase
PRO_0000186450

Regions

Motif613 – 6175DDXXD motif

Sites

Metal binding6131Magnesium 1 By similarity
Metal binding6131Magnesium 2 By similarity
Metal binding6171Magnesium 1 By similarity
Metal binding6171Magnesium 2 By similarity
Metal binding7571Magnesium 3 By similarity
Metal binding7611Magnesium 3 By similarity
Metal binding7651Magnesium 3 By similarity

Experimental info

Sequence conflict1481L → V in AAK83566. Ref.2
Sequence conflict7671A → V in AAK83566. Ref.2

Secondary structure

1 ...................................................................................................... 862
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q41594 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 9141B59780CD79A1

FASTA86298,304
        10         20         30         40         50         60 
MAQLSFNAAL KMNALGNKAI HDPTNCRAKS ERQMMWVCSR SGRTRVKMSR GSGGPGPVVM 

        70         80         90        100        110        120 
MSSSTGTSKV VSETSSTIVD DIPRLSANYH GDLWHHNVIQ TLETPFRESS TYQERADELV 

       130        140        150        160        170        180 
VKIKDMFNAL GDGDISPSAY DTAWVARLAT ISSDGSEKPR FPQALNWVFN NQLQDGSWGI 

       190        200        210        220        230        240 
ESHFSLCDRL LNTTNSVIAL SVWKTGHSQV QQGAEFIAEN LRLLNEEDEL SPDFQIIFPA 

       250        260        270        280        290        300 
LLQKAKALGI NLPYDLPFIK YLSTTREARL TDVSAAADNI PANMLNALEG LEEVIDWNKI 

       310        320        330        340        350        360 
MRFQSKDGSF LSSPASTACV LMNTGDEKCF TFLNNLLDKF GGCVPCMYSI DLLERLSLVD 

       370        380        390        400        410        420 
NIEHLGIGRH FKQEIKGALD YVYRHWSERG IGWGRDSLVP DLNTTALGLR TLRMHGYNVS 

       430        440        450        460        470        480 
SDVLNNFKDE NGRFFSSAGQ THVELRSVVN LFRASDLAFP DERAMDDARK FAEPYLREAL 

       490        500        510        520        530        540 
ATKISTNTKL FKEIEYVVEY PWHMSIPRLE ARSYIDSYDD NYVWQRKTLY RMPSLSNSKC 

       550        560        570        580        590        600 
LELAKLDFNI VQSLHQEELK LLTRWWKESG MADINFTRHR VAEVYFSSAT FEPEYSATRI 

       610        620        630        640        650        660 
AFTKIGCLQV LFDDMADIFA TLDELKSFTE GVKRWDTSLL HEIPECMQTC FKVWFKLMEE 

       670        680        690        700        710        720 
VNNDVVKVQG RDMLAHIRKP WELYFNCYVQ EREWLEAGYI PTFEEYLKTY AISVGLGPCT 

       730        740        750        760        770        780 
LQPILLMGEL VKDDVVEKVH YPSNMFELVS LSWRLTNDTK TYQAEKARGQ QASGIACYMK 

       790        800        810        820        830        840 
DNPGATEEDA IKHICRVVDR ALKEASFEYF KPSNDIPMGC KSFIFNLRLC VQIFYKFIDG 

       850        860 
YGIANEEIKD YIRKVYIDPI QV

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References

[1]"A cDNA clone for taxadiene synthase, the diterpene cyclase that catalyzes the committed step of taxol biosynthesis."
Wildung M.R., Croteau R.B.
J. Biol. Chem. 271:9201-9204(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Genomic organization of plant terpene synthases and molecular evolutionary implications."
Trapp S.C., Croteau R.B.
Genetics 158:811-832(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U48796 mRNA. Translation: AAC49310.1.
AF326519 Genomic DNA. Translation: AAK83566.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3P5PX-ray1.82A108-862[»]
3P5RX-ray2.25A/B108-862[»]
ProteinModelPortalQ41594.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13385.
UniPathwayUPA00842; UER00806.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMSSF48239. Terp_cyc_toroid. 2 hits.
SSF48576. Terpenoid_synth. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ41594.

Entry information

Entry nameTASY_TAXBR
AccessionPrimary (citable) accession number: Q41594
Secondary accession number(s): Q94FV8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: November 1, 1996
Last modified: May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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