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Protein

Taxadiene synthase

Gene

TDC1

Organism
Taxus brevifolia (Pacific yew)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cyclization of the ubiquitous isoprenoid intermediate geranylgeranyl diphosphate to taxa-4,11-diene, the parent olefin with a taxane skeleton.

Catalytic activityi

Geranylgeranyl diphosphate = taxa-4,11-diene + diphosphate.

Cofactori

Mg2+By similarityNote: Binds 3 Mg2+ ions per subunit.By similarity

Pathwayi: taxol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes taxa-4(20),11-dien-5alpha-ol from geranylgeranyl diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Taxadiene synthase (TDC1)
  2. no protein annotated in this organism
This subpathway is part of the pathway taxol biosynthesis, which is itself part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes taxa-4(20),11-dien-5alpha-ol from geranylgeranyl diphosphate, the pathway taxol biosynthesis and in Alkaloid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi613Magnesium 1By similarity1
Metal bindingi613Magnesium 2By similarity1
Metal bindingi617Magnesium 1By similarity1
Metal bindingi617Magnesium 2By similarity1
Metal bindingi757Magnesium 3By similarity1
Metal bindingi761Magnesium 3By similarity1
Metal bindingi765Magnesium 3By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Taxol biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13385.
BRENDAi4.2.3.17. 6223.
UniPathwayiUPA00842; UER00806.

Names & Taxonomyi

Protein namesi
Recommended name:
Taxadiene synthase (EC:4.2.3.17)
Alternative name(s):
Taxa-4(5),11(12)-diene synthase
Gene namesi
Name:TDC1
OrganismiTaxus brevifolia (Pacific yew)
Taxonomic identifieri46220 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaPinidaeCupressalesTaxaceaeTaxus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864501 – 862Taxadiene synthaseAdd BLAST862

Structurei

Secondary structure

1862
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi112 – 128Combined sources17
Helixi139 – 147Combined sources9
Beta strandi149 – 151Combined sources3
Beta strandi157 – 160Combined sources4
Helixi162 – 170Combined sources9
Beta strandi174 – 176Combined sources3
Helixi186 – 202Combined sources17
Helixi207 – 221Combined sources15
Beta strandi226 – 228Combined sources3
Helixi234 – 247Combined sources14
Helixi257 – 274Combined sources18
Helixi282 – 290Combined sources9
Helixi292 – 294Combined sources3
Helixi299 – 303Combined sources5
Helixi314 – 324Combined sources11
Helixi327 – 340Combined sources14
Helixi351 – 365Combined sources15
Helixi368 – 371Combined sources4
Helixi372 – 384Combined sources13
Helixi402 – 414Combined sources13
Helixi421 – 427Combined sources7
Helixi445 – 455Combined sources11
Helixi463 – 482Combined sources20
Beta strandi486 – 488Combined sources3
Helixi489 – 499Combined sources11
Helixi502 – 504Combined sources3
Helixi507 – 517Combined sources11
Beta strandi524 – 530Combined sources7
Turni533 – 535Combined sources3
Helixi538 – 568Combined sources31
Turni571 – 575Combined sources5
Helixi578 – 587Combined sources10
Helixi593 – 595Combined sources3
Helixi596 – 618Combined sources23
Helixi622 – 634Combined sources13
Helixi640 – 642Combined sources3
Helixi645 – 669Combined sources25
Helixi674 – 696Combined sources23
Helixi703 – 713Combined sources11
Helixi716 – 725Combined sources10
Beta strandi727 – 729Combined sources3
Helixi733 – 735Combined sources3
Helixi736 – 739Combined sources4
Helixi744 – 766Combined sources23
Turni767 – 769Combined sources3
Helixi774 – 781Combined sources8
Helixi787 – 810Combined sources24
Beta strandi814 – 816Combined sources3
Helixi818 – 826Combined sources9
Helixi828 – 834Combined sources7
Beta strandi840 – 842Combined sources3
Beta strandi844 – 846Combined sources3
Helixi848 – 856Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P5PX-ray1.82A108-862[»]
3P5RX-ray2.25A/B108-862[»]
ProteinModelPortaliQ41594.
SMRiQ41594.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ41594.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi613 – 617DDXXD motif5

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similaritiesi

Belongs to the terpene synthase family.Curated

Phylogenomic databases

KOiK12921.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Q41594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLSFNAAL KMNALGNKAI HDPTNCRAKS ERQMMWVCSR SGRTRVKMSR
60 70 80 90 100
GSGGPGPVVM MSSSTGTSKV VSETSSTIVD DIPRLSANYH GDLWHHNVIQ
110 120 130 140 150
TLETPFRESS TYQERADELV VKIKDMFNAL GDGDISPSAY DTAWVARLAT
160 170 180 190 200
ISSDGSEKPR FPQALNWVFN NQLQDGSWGI ESHFSLCDRL LNTTNSVIAL
210 220 230 240 250
SVWKTGHSQV QQGAEFIAEN LRLLNEEDEL SPDFQIIFPA LLQKAKALGI
260 270 280 290 300
NLPYDLPFIK YLSTTREARL TDVSAAADNI PANMLNALEG LEEVIDWNKI
310 320 330 340 350
MRFQSKDGSF LSSPASTACV LMNTGDEKCF TFLNNLLDKF GGCVPCMYSI
360 370 380 390 400
DLLERLSLVD NIEHLGIGRH FKQEIKGALD YVYRHWSERG IGWGRDSLVP
410 420 430 440 450
DLNTTALGLR TLRMHGYNVS SDVLNNFKDE NGRFFSSAGQ THVELRSVVN
460 470 480 490 500
LFRASDLAFP DERAMDDARK FAEPYLREAL ATKISTNTKL FKEIEYVVEY
510 520 530 540 550
PWHMSIPRLE ARSYIDSYDD NYVWQRKTLY RMPSLSNSKC LELAKLDFNI
560 570 580 590 600
VQSLHQEELK LLTRWWKESG MADINFTRHR VAEVYFSSAT FEPEYSATRI
610 620 630 640 650
AFTKIGCLQV LFDDMADIFA TLDELKSFTE GVKRWDTSLL HEIPECMQTC
660 670 680 690 700
FKVWFKLMEE VNNDVVKVQG RDMLAHIRKP WELYFNCYVQ EREWLEAGYI
710 720 730 740 750
PTFEEYLKTY AISVGLGPCT LQPILLMGEL VKDDVVEKVH YPSNMFELVS
760 770 780 790 800
LSWRLTNDTK TYQAEKARGQ QASGIACYMK DNPGATEEDA IKHICRVVDR
810 820 830 840 850
ALKEASFEYF KPSNDIPMGC KSFIFNLRLC VQIFYKFIDG YGIANEEIKD
860
YIRKVYIDPI QV
Length:862
Mass (Da):98,304
Last modified:November 1, 1996 - v1
Checksum:i9141B59780CD79A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti148L → V in AAK83566 (PubMed:11404343).Curated1
Sequence conflicti767A → V in AAK83566 (PubMed:11404343).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48796 mRNA. Translation: AAC49310.1.
AF326519 Genomic DNA. Translation: AAK83566.1.

Genome annotation databases

KEGGiag:AAC49310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U48796 mRNA. Translation: AAC49310.1.
AF326519 Genomic DNA. Translation: AAK83566.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3P5PX-ray1.82A108-862[»]
3P5RX-ray2.25A/B108-862[»]
ProteinModelPortaliQ41594.
SMRiQ41594.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAC49310.

Phylogenomic databases

KOiK12921.

Enzyme and pathway databases

UniPathwayiUPA00842; UER00806.
BioCyciMetaCyc:MONOMER-13385.
BRENDAi4.2.3.17. 6223.

Miscellaneous databases

EvolutionaryTraceiQ41594.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.10.20. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 2 hits.
SSF48576. SSF48576. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTASY_TAXBR
AccessioniPrimary (citable) accession number: Q41594
Secondary accession number(s): Q94FV8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.