ID XTH_WHEAT Reviewed; 293 AA. AC Q41542; DT 28-NOV-2003, integrated into UniProtKB/Swiss-Prot. DT 01-NOV-1996, sequence version 1. DT 27-MAR-2024, entry version 111. DE RecName: Full=Probable xyloglucan endotransglucosylase/hydrolase; DE EC=2.4.1.207; DE Flags: Precursor; GN Name=XTH; Synonyms=EXT; OS Triticum aestivum (Wheat). OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum. OX NCBI_TaxID=4565; RN [1] RP NUCLEOTIDE SEQUENCE [MRNA]. RX PubMed=8244968; DOI=10.1016/s0021-9258(19)74400-7; RA Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., RA Nishitani K.; RT "Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a RT novel class of glycosyltransferase that mediates molecular grafting between RT matrix polysaccharides in plant cell walls."; RL J. Biol. Chem. 268:25364-25368(1993). CC -!- FUNCTION: Catalyzes xyloglucan endohydrolysis (XEH) and/or CC endotransglycosylation (XET). Cleaves and religates xyloglucan CC polymers, an essential constituent of the primary cell wall, and CC thereby participates in cell wall construction of growing tissues (By CC similarity). {ECO:0000250}. CC -!- CATALYTIC ACTIVITY: CC Reaction=breaks a beta-(1->4) bond in the backbone of a xyloglucan and CC transfers the xyloglucanyl segment on to O-4 of the non-reducing CC terminal glucose residue of an acceptor, which can be a xyloglucan or CC an oligosaccharide of xyloglucan.; EC=2.4.1.207; CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000305}. Secreted, CC extracellular space, apoplast {ECO:0000305}. CC -!- PTM: Contains at least one intrachain disulfide bond essential for its CC enzymatic activity. {ECO:0000250}. CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 16 family. XTH group 1 CC subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; D16457; BAA03924.1; -; mRNA. DR PIR; E49539; E49539. DR AlphaFoldDB; Q41542; -. DR SMR; Q41542; -. DR STRING; 4565.Q41542; -. DR CAZy; GH16; Glycoside Hydrolase Family 16. DR GlyCosmos; Q41542; 1 site, No reported glycans. DR PaxDb; 4565-Traes_4DS_6B94D4FA6-1; -. DR EnsemblPlants; TraesKAR4A01G0267150.1; cds.TraesKAR4A01G0267150.1; TraesKAR4A01G0267150. DR Gramene; TraesKAR4A01G0267150.1; cds.TraesKAR4A01G0267150.1; TraesKAR4A01G0267150. DR eggNOG; ENOG502QQUC; Eukaryota. DR Proteomes; UP000019116; Unplaced. DR ExpressionAtlas; Q41542; baseline and differential. DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell. DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro. DR GO; GO:0030247; F:polysaccharide binding; ISS:UniProtKB. DR GO; GO:0016762; F:xyloglucan:xyloglucosyl transferase activity; IEA:UniProtKB-EC. DR GO; GO:0042546; P:cell wall biogenesis; IEA:InterPro. DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW. DR GO; GO:0010411; P:xyloglucan metabolic process; IEA:InterPro. DR CDD; cd02176; GH16_XET; 1. DR Gene3D; 2.60.120.200; -; 1. DR InterPro; IPR044791; Beta-glucanase/XTH. DR InterPro; IPR013320; ConA-like_dom_sf. DR InterPro; IPR000757; GH16. DR InterPro; IPR008263; GH16_AS. DR InterPro; IPR010713; XET_C. DR InterPro; IPR016455; XTH. DR PANTHER; PTHR31062; XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 8-RELATED; 1. DR PANTHER; PTHR31062:SF243; XYLOGLUCAN ENDOTRANSGLUCOSYLASE_HYDROLASE-RELATED; 1. DR Pfam; PF00722; Glyco_hydro_16; 1. DR Pfam; PF06955; XET_C; 1. DR PIRSF; PIRSF005604; XET; 1. DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1. DR PROSITE; PS01034; GH16_1; 1. DR PROSITE; PS51762; GH16_2; 1. PE 2: Evidence at transcript level; KW Apoplast; Cell wall; Cell wall biogenesis/degradation; Disulfide bond; KW Glycoprotein; Glycosidase; Hydrolase; Reference proteome; Secreted; Signal; KW Transferase. FT SIGNAL 1..22 FT /evidence="ECO:0000255" FT CHAIN 23..293 FT /note="Probable xyloglucan endotransglucosylase/hydrolase" FT /id="PRO_0000011841" FT DOMAIN 23..220 FT /note="GH16" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01098" FT ACT_SITE 106 FT /note="Nucleophile" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10064" FT ACT_SITE 110 FT /note="Proton donor" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10064" FT BINDING 110 FT /ligand="xyloglucan" FT /ligand_id="ChEBI:CHEBI:18233" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT BINDING 123..125 FT /ligand="xyloglucan" FT /ligand_id="ChEBI:CHEBI:18233" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT BINDING 133..135 FT /ligand="xyloglucan" FT /ligand_id="ChEBI:CHEBI:18233" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT BINDING 199..200 FT /ligand="xyloglucan" FT /ligand_id="ChEBI:CHEBI:18233" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT BINDING 204 FT /ligand="xyloglucan" FT /ligand_id="ChEBI:CHEBI:18233" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT BINDING 279 FT /ligand="xyloglucan" FT /ligand_id="ChEBI:CHEBI:18233" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT SITE 108 FT /note="Important for catalytic activity" FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT CARBOHYD 114 FT /note="N-linked (GlcNAc...) asparagine" FT /evidence="ECO:0000255" FT DISULFID 228..237 FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" FT DISULFID 274..287 FT /evidence="ECO:0000250|UniProtKB:Q8GZD5" SQ SEQUENCE 293 AA; 33422 MW; FF21C732308DB446 CRC64; MKATAGALLA VVAAVLLRGV AAAPRKPVDV PFDKNYVPTW AQDHIHYVNG GREVQLSLDK TTGTGFQTRG SYLFGHFSMH IKLVGGDSAG TVTAFYLSSQ NSEHDEIDFE FLGNRTGQPY ILQTNVFSGG KGDREQRIYL WFDPTKDYHS YSVLWNLYMI AFFVDDTPIR VFKNSKDLGV RYPFDQPMKL YSSLWNADDW ATRGGREKTD WSKAPFVASY RGFHVDGCEA SAEAKFCATQ GARWWDQPEF QDLDAAQYRR LAWVRKEHTI YNYCTDHDRY AAMAPECKRD RDV //