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Protein

Probable xyloglucan endotransglucosylase/hydrolase

Gene

XTH

Organism
Triticum aestivum (Wheat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity).By similarity

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei106NucleophilePROSITE-ProRule annotation1
Active sitei110Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase, Transferase
Biological processCell wall biogenesis/degradation

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase (EC:2.4.1.207)
Gene namesi
Name:XTH
Synonyms:EXT
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeTriticinaeTriticum
Proteomesi
  • UP000019116 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000001184123 – 293Probable xyloglucan endotransglucosylase/hydrolaseAdd BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ41542.

Expressioni

Gene expression databases

ExpressionAtlasiQ41542. baseline and differential.

Structurei

3D structure databases

ProteinModelPortaliQ41542.
SMRiQ41542.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini23 – 220GH16PROSITE-ProRule annotationAdd BLAST198

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR013320. ConA-like_dom.
IPR000757. GH16.
IPR008263. GH16_AS.
IPR010713. XET_C.
IPR016455. XTH.
PfamiView protein in Pfam
PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.
PROSITEiView protein in PROSITE
PS01034. GH16_1. 1 hit.
PS51762. GH16_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q41542-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKATAGALLA VVAAVLLRGV AAAPRKPVDV PFDKNYVPTW AQDHIHYVNG
60 70 80 90 100
GREVQLSLDK TTGTGFQTRG SYLFGHFSMH IKLVGGDSAG TVTAFYLSSQ
110 120 130 140 150
NSEHDEIDFE FLGNRTGQPY ILQTNVFSGG KGDREQRIYL WFDPTKDYHS
160 170 180 190 200
YSVLWNLYMI AFFVDDTPIR VFKNSKDLGV RYPFDQPMKL YSSLWNADDW
210 220 230 240 250
ATRGGREKTD WSKAPFVASY RGFHVDGCEA SAEAKFCATQ GARWWDQPEF
260 270 280 290
QDLDAAQYRR LAWVRKEHTI YNYCTDHDRY AAMAPECKRD RDV
Length:293
Mass (Da):33,422
Last modified:November 1, 1996 - v1
Checksum:iFF21C732308DB446
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16457 mRNA. Translation: BAA03924.1.
PIRiE49539.
UniGeneiTa.54979.

Similar proteinsi

Entry informationi

Entry nameiXTH_WHEAT
AccessioniPrimary (citable) accession number: Q41542
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 1, 1996
Last modified: October 25, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families