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Q41542 (XTH_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable xyloglucan endotransglucosylase/hydrolase

EC=2.4.1.207
Gene names
Name:XTH
Synonyms:EXT
OrganismTriticum aestivum (Wheat) [Complete proteome]
Taxonomic identifier4565 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.

Catalytic activity

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Subcellular location

Secretedcell wall Probable. Secretedextracellular spaceapoplast Probable.

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.

Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
   Cellular componentApoplast
Cell wall
Secreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
Transferase
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcellular glucan metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionhydrolase activity, hydrolyzing O-glycosyl compounds

Inferred from electronic annotation. Source: InterPro

xyloglucan:xyloglucosyl transferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 293271Probable xyloglucan endotransglucosylase/hydrolase
PRO_0000011841

Sites

Active site1061Nucleophile By similarity
Active site1101Proton donor By similarity

Amino acid modifications

Glycosylation1141N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q41542 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: FF21C732308DB446

FASTA29333,422
        10         20         30         40         50         60 
MKATAGALLA VVAAVLLRGV AAAPRKPVDV PFDKNYVPTW AQDHIHYVNG GREVQLSLDK 

        70         80         90        100        110        120 
TTGTGFQTRG SYLFGHFSMH IKLVGGDSAG TVTAFYLSSQ NSEHDEIDFE FLGNRTGQPY 

       130        140        150        160        170        180 
ILQTNVFSGG KGDREQRIYL WFDPTKDYHS YSVLWNLYMI AFFVDDTPIR VFKNSKDLGV 

       190        200        210        220        230        240 
RYPFDQPMKL YSSLWNADDW ATRGGREKTD WSKAPFVASY RGFHVDGCEA SAEAKFCATQ 

       250        260        270        280        290 
GARWWDQPEF QDLDAAQYRR LAWVRKEHTI YNYCTDHDRY AAMAPECKRD RDV 

« Hide

References

[1]"Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls."
Okazawa K., Sato Y., Nakagawa T., Asada K., Kato I., Tomita E., Nishitani K.
J. Biol. Chem. 268:25364-25368(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D16457 mRNA. Translation: BAA03924.1.
PIRE49539.
UniGeneTa.54979.

3D structure databases

ProteinModelPortalQ41542.
SMRQ41542. Positions 27-293.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH16. Glycoside Hydrolase Family 16.

Proteomic databases

PRIDEQ41542.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneQ41542.

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000757. Glyco_hydro_16.
IPR008263. Glycoside_hydrolase_16_AS.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFPIRSF005604. XET. 1 hit.
SUPFAMSSF49899. SSF49899. 1 hit.
PROSITEPS01034. GLYCOSYL_HYDROL_F16. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXTH_WHEAT
AccessionPrimary (citable) accession number: Q41542
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries