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Q41452 (FLS_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Flavonol synthase/flavanone 3-hydroxylase
Short name=FLS
EC=1.14.11.23
EC=1.14.11.9
OrganismSolanum tuberosum (Potato) [Reference proteome]
Taxonomic identifier4113 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin.

Catalytic activity

A dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O.

A flavanone + 2-oxoglutarate + O2 = a dihydroflavonol + succinate + CO2.

Cofactor

Binds 1 iron ion per subunit By similarity.

Binds 1 ascorbate molecule per subunit By similarity.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Subcellular location

Cytoplasm.

Developmental stage

Temporally expressed during flower development.

Sequence similarities

Belongs to the iron/ascorbate-dependent oxidoreductase family.

Contains 1 Fe2OG dioxygenase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Flavonol synthase/flavanone 3-hydroxylase
PRO_0000067297

Regions

Domain213 – 31098Fe2OG dioxygenase

Sites

Metal binding2381Iron By similarity
Metal binding2401Iron By similarity
Metal binding2911Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
Q41452 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: ADBBC3F6B10A0E05

FASTA34939,728
        10         20         30         40         50         60 
MKTIQGQSAT TALTMEVARV QAISSITKCM DTIPSEYIRS ENEQPAATTL QGVVLEVPVI 

        70         80         90        100        110        120 
DISNVDDDEE KLVKEIVEAS KEWGIFQVIN HGIPDEVIEN LQKVGKEFFE EVPQEEKELI 

       130        140        150        160        170        180 
AKKPGAQSLE GYGTSLQKEI EGKKGWVDHL FHKIWPPSAI NYRYWPKNPP SYREANEEYA 

       190        200        210        220        230        240 
KWLRKVADGI FRSLSLGLGL EGHEMMEAAG SEDIVYMLKI NYYPPCPRPD LALGVVAHTD 

       250        260        270        280        290        300 
MSYITLLVPN EVQVFKDGHW YDVNYIPNAI IVHIGDQVEI LSNGKYKSVY HRTTVNKYKT 

       310        320        330        340 
RMSWPVFLEP SSEHEVGPIP NLINEANPPK FKTKKYKDYV YCKLNKLPQ

« Hide

References

[1]"Regulation of flavonol biosynthesis during anther and pistil development, and during pollen tube growth in Solanum tuberosum."
van Eldik G.J., Ruiter R.K., Reijnen W.H., van Herpen M.M.A., Schrauwen J.A.M., Wullems G.J.
Plant J. 11:105-113(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pistil.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X92178 mRNA. Translation: CAA63092.1.
PIRT07373.

3D structure databases

ProteinModelPortalQ41452.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00154.

Family and domain databases

InterProIPR026992. DIOX_N.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PROSITEPS51471. FE2OG_OXY. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFLS_SOLTU
AccessionPrimary (citable) accession number: Q41452
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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