Reviewed, UniProtKB/Swiss-Prot Q41452 (FLS_SOLTU)

Names and origin

Protein names	Recommended name:     Flavonol synthase/flavanone 3-hydroxylase       Short name=FLS     EC=1.14.11.23     EC=1.14.11.9
Organism	Solanum tuberosum (Potato)
Taxonomic identifier	4113 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of flavonols from dihydroflavonols. It can act on dihydrokaempferol to produce kaempferol, on dihydroquercetin to produce quercitin and on dihydromyricetin to produce myricetin.
Catalytic activity	A dihydroflavonol + 2-oxoglutarate + O(2) = a flavonol + succinate + CO(2) + H(2)O. A flavanone + 2-oxoglutarate + O(2) = a dihydroflavonol + succinate + CO(2).
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 ascorbate molecule per subunit By similarity.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Subcellular location	Cytoplasm.
Developmental stage	Temporally expressed during flower development.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW flavonol synthase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: InterPro naringenin 3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Flavonol synthase/flavanone 3-hydroxylase		PRO_0000067297
Sites
	Metal binding	238	1	Iron By similarity
	Metal binding	240	1	Iron By similarity
	Metal binding	291	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q41452-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: ADBBC3F6B10A0E05	FASTA	349	39,728
	10 20 30 40 50 60 MKTIQGQSAT TALTMEVARV QAISSITKCM DTIPSEYIRS ENEQPAATTL QGVVLEVPVI 70 80 90 100 110 120 DISNVDDDEE KLVKEIVEAS KEWGIFQVIN HGIPDEVIEN LQKVGKEFFE EVPQEEKELI 130 140 150 160 170 180 AKKPGAQSLE GYGTSLQKEI EGKKGWVDHL FHKIWPPSAI NYRYWPKNPP SYREANEEYA 190 200 210 220 230 240 KWLRKVADGI FRSLSLGLGL EGHEMMEAAG SEDIVYMLKI NYYPPCPRPD LALGVVAHTD 250 260 270 280 290 300 MSYITLLVPN EVQVFKDGHW YDVNYIPNAI IVHIGDQVEI LSNGKYKSVY HRTTVNKYKT 310 320 330 340 RMSWPVFLEP SSEHEVGPIP NLINEANPPK FKTKKYKDYV YCKLNKLPQ

References

[1]

"Regulation of flavonol biosynthesis during anther and pistil development, and during pollen tube growth in Solanum tuberosum." van Eldik G.J., Ruiter R.K., Reijnen W.H., van Herpen M.M.A., Schrauwen J.A.M., Wullems G.J. Plant J. 11:105-113(1997) [PubMed: 9025306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pistil.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X92178 mRNA. Translation: CAA63092.1.
PIR	T07373.
3D structure databases
HSSP	HSSP built from PDB template 1GP6 based on UniProtKB Q96323.
ModBase	Search...
Family and domain databases
InterPro	IPR005123. 2OG-FeII_Oase. IPR002283. Isopenicillin-N_synthase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
ProtoNet	Search...

Entry information

Entry name

FLS_SOLTU

Accession

Primary (citable) accession number: Q41452

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	November 25, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)