ID   HMDH3_SOLTU             Reviewed;         574 AA.
AC   Q41438;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 69.
DE   RecName: Full=3-hydroxy-3-methylglutaryl-coenzyme A reductase 3;
DE            Short=HMG-CoA reductase 3;
DE            EC=1.1.1.34;
DE   AltName: Full=HMG3.3;
GN   Name=HMG3;
OS   Solanum tuberosum (Potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae;
OC   Solanum.
OX   NCBI_TaxID=4113;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Kennebec; TISSUE=Tuber;
RX   MEDLINE=97201488; PubMed=9049274; DOI=10.1023/A:1005743011651;
RA   Korth K.L., Stermer B.A., Bhattacharyya M.K., Dixon R.A.;
RT   "HMG-CoA reductase gene families that differentially accumulate
RT   transcripts in potato tubers are developmentally expressed in floral
RT   tissues.";
RL   Plant Mol. Biol. 33:545-551(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Itoh Y., Yoshioka H., Doke N.;
RT   "Structure and nucleotide sequence of potato HMG3 encoding 3-hydroxy-
RT   3-methylglutaryl coenzyme A reductase.";
RL   Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the synthesis of mevalonate. The specific
CC       precursor of all isoprenoid compounds present in plants.
CC   -!- CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP(+) = (S)-3-
CC       hydroxy-3-methylglutaryl-CoA + 2 NADPH.
CC   -!- PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid
CC       biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass
CC       membrane protein.
CC   -!- TISSUE SPECIFICITY: Expressed in mature petals and anthers.
CC   -!- SIMILARITY: Belongs to the HMG-CoA reductase family.
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DR   EMBL; U51986; AAB52552.1; -; mRNA.
DR   EMBL; AB022690; BAA93631.1; -; Genomic_DNA.
DR   PIR; S59946; S59946.
DR   PIR; T07112; T07112.
DR   HSSP; P04035; 1HW8.
DR   BRENDA; 1.1.1.34; 296.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004420; F:hydroxymethylglutaryl-CoA reductase (NADPH)...; IEA:EC.
DR   GO; GO:0050661; F:NADP or NADPH binding; IEA:InterPro.
DR   GO; GO:0015936; P:coenzyme A metabolic process; IEA:InterPro.
DR   GO; GO:0008299; P:isoprenoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR002202; HMG_CoA_Rdtase_cat.
DR   InterPro; IPR004554; HMG_CoA_Rdtase_I_cat.
DR   Gene3D; G3DSA:3.90.770.10; HMG-CoA_red; 1.
DR   PANTHER; PTHR10572; HMG-CoA_red; 1.
DR   Pfam; PF00368; HMG-CoA_red; 1.
DR   PRINTS; PR00071; HMGCOARDTASE.
DR   TIGRFAMs; TIGR00533; HMG_CoA_R_NADP; 1.
DR   PROSITE; PS00066; HMG_COA_REDUCTASE_1; 1.
DR   PROSITE; PS00318; HMG_COA_REDUCTASE_2; 1.
DR   PROSITE; PS01192; HMG_COA_REDUCTASE_3; 1.
DR   PROSITE; PS50065; HMG_COA_REDUCTASE_4; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; Isoprene biosynthesis; Membrane;
KW   NADP; Oxidoreductase; Transmembrane.
FT   CHAIN         1    574       3-hydroxy-3-methylglutaryl-coenzyme A
FT                                reductase 3.
FT                                /FTId=PRO_0000114450.
FT   TRANSMEM     41     61       Potential.
FT   TRANSMEM     83    103       Potential.
FT   REGION      104    161       Linker (By similarity).
FT   REGION      162    574       Catalytic (By similarity).
FT   ACT_SITE    256    256       Charge relay system (By similarity).
FT   ACT_SITE    388    388       Charge relay system (By similarity).
FT   ACT_SITE    464    464       Charge relay system (By similarity).
FT   ACT_SITE    562    562       Proton donor (By similarity).
FT   CARBOHYD     78     78       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    113    113       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    320    320       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    433    433       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    566    566       N-linked (GlcNAc...) (Potential).
SQ   SEQUENCE   574 AA;  62159 MW;  E020BB10EF4779E4 CRC64;
     MDVRRRPVKP LYPSEHISSG EPLKPHNQDS SVKASDALPL PLYLTNGLFF TMFFSVMYFL
     LHRWREKIRN GIPLHVLNFS ELVAMVSLIA SVIYLLGFFG IGFVQSFVSK GNNDSWDVED
     ESPEQFIDRT VTPPPVRRNI PMKSVPVAEK TAQIITPFSS EDDEVVIKSV VEGRIPSYSL
     ESKLGDCKRA AFIRKEALQR SSGKSLEGLP LDGFDYESIL GQCCEMPIGY IQIPVGIAGP
     LLLNGKEFSV PMATTEGCLV ASTNRGCKAI YVSGGATSVL FRDAMTRAPV VRFGSAKRAA
     ELKFFVEDPM NFETLSVVFN KSSRFARLQN IQCAIAGKNL YMRFSCSTGD AMGMNMVSKG
     VQNVLDYLQN EYPDMDIIGI SGNYCSDKKP AAVNWIEGRG KSVVCEAIIK EDVVKKVLKT
     EVATLVELNM LKNLTGSAMA GALGGFNAHA SNIVSAVYLA TGQDPAQNIE SSHCITMMEA
     VNDGKDLHIS VTMPSIEVGT VGGGTQLASQ SACLNLLGVK GANREAPGSN ARLLATIVAG
     SVLAGELSLM SAISAGQLVK SHMKYNRSCK DVTK
//