Reviewed, UniProtKB/Swiss-Prot Q41438 (HMDH3_SOLTU)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase 3
      Short name=HMG-CoA reductase 3
    EC=1.1.1.34
Alternative name(s):
    HMG3.3

Gene names

Name:

HMG3

Organism

Solanum tuberosum (Potato)

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in mature petals and anthers.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 574	574	3-hydroxy-3-methylglutaryl-coenzyme A reductase 3		PRO_0000114450
Regions
	Transmembrane	41 – 61	21	Potential
	Transmembrane	83 – 103	21	Potential
	Region	104 – 161	58	Linker By similarity
	Region	162 – 574	413	Catalytic By similarity
Sites
	Active site	256	1	Charge relay system By similarity
	Active site	388	1	Charge relay system By similarity
	Active site	464	1	Charge relay system By similarity
	Active site	562	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	78	1	N-linked (GlcNAc...) Potential
	Glycosylation	113	1	N-linked (GlcNAc...) Potential
	Glycosylation	320	1	N-linked (GlcNAc...) Potential
	Glycosylation	433	1	N-linked (GlcNAc...) Potential
	Glycosylation	566	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q41438-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E020BB10EF4779E4	FASTA	574	62,159
	10 20 30 40 50 60 MDVRRRPVKP LYPSEHISSG EPLKPHNQDS SVKASDALPL PLYLTNGLFF TMFFSVMYFL 70 80 90 100 110 120 LHRWREKIRN GIPLHVLNFS ELVAMVSLIA SVIYLLGFFG IGFVQSFVSK GNNDSWDVED 130 140 150 160 170 180 ESPEQFIDRT VTPPPVRRNI PMKSVPVAEK TAQIITPFSS EDDEVVIKSV VEGRIPSYSL 190 200 210 220 230 240 ESKLGDCKRA AFIRKEALQR SSGKSLEGLP LDGFDYESIL GQCCEMPIGY IQIPVGIAGP 250 260 270 280 290 300 LLLNGKEFSV PMATTEGCLV ASTNRGCKAI YVSGGATSVL FRDAMTRAPV VRFGSAKRAA 310 320 330 340 350 360 ELKFFVEDPM NFETLSVVFN KSSRFARLQN IQCAIAGKNL YMRFSCSTGD AMGMNMVSKG 370 380 390 400 410 420 VQNVLDYLQN EYPDMDIIGI SGNYCSDKKP AAVNWIEGRG KSVVCEAIIK EDVVKKVLKT 430 440 450 460 470 480 EVATLVELNM LKNLTGSAMA GALGGFNAHA SNIVSAVYLA TGQDPAQNIE SSHCITMMEA 490 500 510 520 530 540 VNDGKDLHIS VTMPSIEVGT VGGGTQLASQ SACLNLLGVK GANREAPGSN ARLLATIVAG 550 560 570 SVLAGELSLM SAISAGQLVK SHMKYNRSCK DVTK

References

[1]	"HMG-CoA reductase gene families that differentially accumulate transcripts in potato tubers are developmentally expressed in floral tissues." Korth K.L., Stermer B.A., Bhattacharyya M.K., Dixon R.A. Plant Mol. Biol. 33:545-551(1997) [PubMed: 9049274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Kennebec. Tissue: Tuber.
[2]	"Structure and nucleotide sequence of potato HMG3 encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase." Itoh Y., Yoshioka H., Doke N. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U51986 mRNA. Translation: AAB52552.1. AB022690 Genomic DNA. Translation: BAA93631.1.
PIR	S59946. T07112.
3D structure databases
HSSP	HSSP built from PDB template 1HW8 based on UniProtKB P04035.
ModBase	Search...
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH3_SOLTU

Accession

Primary (citable) accession number: Q41438

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)