Q41437 (HMDH2_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
Short name=HMG-CoA reductase 2
EC=1.1.1.34

Alternative name(s):
HMG2.2

Gene names

Name:

HMG2

Organism

Solanum tuberosum (Potato)

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	595 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Tissue specificity	Expressed in young flowers and in mature sepals and ovaries.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 595	595	3-hydroxy-3-methylglutaryl-coenzyme A reductase 2		PRO_0000114449
Regions
	Transmembrane	48 – 68	21	Helical; Potential
	Transmembrane	92 – 112	21	Helical; Potential
	Region	113 – 183	71	Linker By similarity
	Region	184 – 595	412	Catalytic By similarity
Sites
	Active site	278	1	Charge relay system By similarity
	Active site	410	1	Charge relay system By similarity
	Active site	486	1	Charge relay system By similarity
	Active site	584	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	35	1	N-linked (GlcNAc...) Potential
	Glycosylation	121	1	N-linked (GlcNAc...) Potential
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
	Glycosylation	455	1	N-linked (GlcNAc...) Potential
	Glycosylation	588	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q41437 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0FA7069849D41D57	FASTA	595	63,842
	10 20 30 40 50 60 MDVRRRSEKP VYPSKVFGAD EKPLKPHNNQ QQEDNNTLLI DASDALPLPL YLTNGLFFTM 70 80 90 100 110 120 FFSVMYFLLS RWREKIRNST PLHVVTLSEL GAIVSLIASV IYLLGFFGIG FVQTFVSRGN 130 140 150 160 170 180 NDSWDENDEE FLLKEDSRCG PATTLGCAIP APPARQISPM APPQPAMSMV EKPSPLITPA 190 200 210 220 230 240 SSEEDEEIIN SVVQGKFPSY SLVIQLGDVS AAASLRKEVM QRITGKSLEG LPLEGFTYES 250 260 270 280 290 300 ILGQCCEMPI GYVQIPVGIA GPLLLNGKEF SVPMATTEGC LVASTNRGCK AIYASGGATC 310 320 330 340 350 360 IVLRDGMTRA PCVRFGTAKR AAELKFFVED PIKFETLANV FNQSSRFGRL QRIQCAIAGK 370 380 390 400 410 420 NLYMRFVCST GDAMGMNMVS KGVQNVLDYL QNEYPDMDVI GISGNFCSDK KPAAVNWIEG 430 440 450 460 470 480 RGKSVVCEAI ITEEVVKKVL KTEVAALVEL NMLKNLTGSA MAGALGGFNA HASNIVSAVF 490 500 510 520 530 540 IATGQDPAQN IESSHCITMM EAVNDGKDLH ISVTMPSIEV GTVGGGTQLA SQSACLNLLG 550 560 570 580 590 VKGANREAPG SNARLLATVV AGSVLAGELS LMSAISAGQL VNSHMKYNRS TKASS

References

[1]	"HMG-CoA reductase gene families that differentially accumulate transcripts in potato tubers are developmentally expressed in floral tissues." Korth K.L., Stermer B.A., Bhattacharyya M.K., Dixon R.A. Plant Mol. Biol. 33:545-551(1997) [PubMed: 9049274] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Kennebec. Tissue: Tuber.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U51985 mRNA. Translation: AAB52551.1.
3D structure databases
ProteinModelPortal	Q41437.
SMR	Q41437. Positions 181-581.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase. IPR023074. HMG_CoA_Rdtase_cat. IPR023076. HMG_CoA_Rdtase_CS. IPR004554. HMG_CoA_Rdtase_eu_arc. IPR023282. HMG_CoA_Rdtase_N. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view]
Gene3D	G3DSA:3.30.70.420. G3DSA:3.30.70.420. 1 hit. G3DSA:3.90.770.10. HMG-CoA_red. 2 hits. G3DSA:1.10.3270.10. HMG_CoA_Rdtase_N. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
SUPFAM	SSF55035. HMG_CoA_NAD_bind. 1 hit. SSF56542. HMG_CoA_sub_bind. 1 hit.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH2_SOLTU

Accession

Primary (citable) accession number: Q41437

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	June 28, 2011
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program