Q41389 (RIP5_SAPOF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosome-inactivating protein saporin-5
Short name=SAP-5
EC=3.2.2.22

Alternative name(s):
rRNA N-glycosidase

Gene names

Name:

SAP5

Organism

Saponaria officinalis (Common soapwort) (Lychnis saponaria)

Taxonomic identifier

3572 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Saponaria

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells By similarity.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Ribosome-inactivating protein saporin-5		PRO_0000221413
Sites
	Active site	176	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q41389 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 539693E13D0F594D	FASTA	253	28,557
	10 20 30 40 50 60 VTSITLDLVN PTAGQYSSFV DKIRNNVKDP NLKYGGTDIA VIGPPSKEKF LRINFQSSRG 70 80 90 100 110 120 TVSLGLKRDN LYVVAYLAMD NTNVNRAYYF RSEITSAELT ALFPEATTAN QKALEYTEDY 130 140 150 160 170 180 QSIEKNAQIT QGDKSRKELG LGIDLLLTSM EAVNKKARVV KNEARFLLIA IQMTAEVARF 190 200 210 220 230 240 RYIQNLVTKN FPNKFDSDNK VIQFEVSWRK ISTAIYGDAK NGVFNKDYDF GFGKVRQVKD 250 LQMGLLMYLG KPK

References

[1]	"The expression of saporin, a ribosome-inactivating protein from the plant Saponaria officinalis, in Escherichia coli." Barthelemy I., Martineau D., Ong M., Matsunami R., Ling N., Benatti L., Cavallaro U., Soria M., Lappi D.A. J. Biol. Chem. 268:6541-6548(1993) [PubMed: 8454624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X69131 Genomic DNA. Translation: CAA48885.1. X69132 Genomic DNA. Translation: CAA48886.1.
PIR	S16487. S28539. S38527.
3D structure databases
ProteinModelPortal	Q41389.
SMR	Q41389. Positions 1-253.
ModBase	Search...
Protein family/group databases
Allergome	2805. Sap o RIP.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit. G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
SUPFAM	SSF56371. Ribosome_inactivat_prot. 1 hit.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP5_SAPOF

Accession

Primary (citable) accession number: Q41389

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	November 1, 1996
Last modified:	June 28, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program