ID   PORA_CUCSA              Reviewed;         398 AA.
AC   Q41249;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   24-JAN-2024, entry version 111.
DE   RecName: Full=Protochlorophyllide reductase, chloroplastic;
DE            Short=PCR;
DE            EC=1.3.1.33;
DE   AltName: Full=NADPH-protochlorophyllide oxidoreductase;
DE            Short=POR;
DE   Flags: Precursor;
GN   Name=PORA; Synonyms=NPR;
OS   Cucumis sativus (Cucumber).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX   NCBI_TaxID=3659;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Aonagajibai; TISSUE=Cotyledon;
RX   PubMed=7755605; DOI=10.1006/bbrc.1995.1662;
RA   Kuroda H., Masuda T., Ohta H., Shioi Y., Takamiya K.;
RT   "Light-enhanced gene expression of NADPH-protochlorophyllide oxidoreductase
RT   in cucumber.";
RL   Biochem. Biophys. Res. Commun. 210:310-316(1995).
CC   -!- FUNCTION: Phototransformation of protochlorophyllide (Pchlide) to
CC       chlorophyllide (Chlide).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=chlorophyllide a + NADP(+) = H(+) + NADPH +
CC         protochlorophyllide a; Xref=Rhea:RHEA:11132, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:83348,
CC         ChEBI:CHEBI:83350; EC=1.3.1.33;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. POR subfamily. {ECO:0000305}.
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DR   EMBL; D50085; BAA21089.1; -; mRNA.
DR   PIR; JC4146; JC4146.
DR   RefSeq; NP_001267713.1; NM_001280784.1.
DR   AlphaFoldDB; Q41249; -.
DR   SMR; Q41249; -.
DR   GeneID; 101208554; -.
DR   KEGG; csv:101208554; -.
DR   eggNOG; KOG1208; Eukaryota.
DR   OrthoDB; 713at2759; -.
DR   BRENDA; 1.3.1.33; 1733.
DR   UniPathway; UPA00668; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0016630; F:protochlorophyllide reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   CDD; cd09810; LPOR_like_SDR_c_like; 1.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR005979; Prochl_reduct.
DR   InterPro; IPR002347; SDR_fam.
DR   NCBIfam; TIGR01289; LPOR; 1.
DR   PANTHER; PTHR44419:SF19; PROTOCHLOROPHYLLIDE REDUCTASE A, CHLOROPLASTIC; 1.
DR   PANTHER; PTHR44419; PROTOCHLOROPHYLLIDE REDUCTASE C, CHLOROPLASTIC; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   2: Evidence at transcript level;
KW   Chlorophyll biosynthesis; Chloroplast; NADP; Oxidoreductase;
KW   Photosynthesis; Plastid; Transit peptide.
FT   TRANSIT         1..64
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000250"
FT   CHAIN           65..398
FT                   /note="Protochlorophyllide reductase, chloroplastic"
FT                   /id="PRO_0000023291"
SQ   SEQUENCE   398 AA;  43074 MW;  BE9041148AB0EB0A CRC64;
     MALQAASLVS PALSIPKEGK SSVCLKDSSL FGISFSDHLK SEFSSSTLRC KRELNQQIGA
     IRAQTTATES PAVNKATPDG KKTLRKGSVV ITGASSGLGL ATAKALAETG KWHVIMACRD
     FLKAERAAKS AGITKENYTV MHLDLASLDS VRQFVDNFRQ SGRPLDVLVC NAAVYLPTAK
     EPTFTAEGFE LSVGTNHLGH FLLSRLLLED LNKSSYPSKR LIIVGSITGN TNTLAGNVPP
     KANLGDLRGL AGGLNGLKSS MIDGGEFDGA KAYKDSKVCN MLTMQEFHKR YHEETGITFA
     SLYPGCIATT GLFREHIPLF RILFPPFQKF ITQGYVSEDE AGKRLAQVVS EPSLTKSGVY
     WSWNKNSASF ENQLSQEASD AEKARKVWEL SEKLVGLA
//