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Q41233

- ALLN2_ALLSA

UniProt

Q41233 - ALLN2_ALLSA

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Protein

Alliin lyase 2

Gene
N/A
Organism
Allium sativum (Garlic)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Able to cleave the C-S bond of sulfoxide derivatives of Cys to produce allicin, thus giving rise to all sulfur compounds which are responsible for most of the properties of garlic, such as the specific smell and flavor as well as the health benefits like blood lipid or blood pressure lowering.

Catalytic activityi

An S-alkyl-L-cysteine S-oxide = an alkyl sulfenate + 2-aminoacrylate.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei119 – 1191ChlorideBy similarity
Binding sitei123 – 1231ChlorideBy similarity
Binding sitei125 – 1251ChlorideBy similarity

GO - Molecular functioni

  1. alliin lyase activity Source: UniProtKB-EC
  2. pyridoxal phosphate binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Chloride, Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13491.

Names & Taxonomyi

Protein namesi
Recommended name:
Alliin lyase 2 (EC:4.4.1.4)
Short name:
Alliinase-2
Alternative name(s):
Cysteine sulphoxide lyase 2
OrganismiAllium sativum (Garlic)
Taxonomic identifieri4682 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesAmaryllidaceaeAllioideaeAllieaeAllium

Subcellular locationi

GO - Cellular componenti

  1. vacuole Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Protein family/group databases

Allergomei843. All s Alliin lyase.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Sequence AnalysisAdd
BLAST
Propeptidei16 – 2510By similarityPRO_0000020679
Chaini26 – 473448Alliin lyase 2PRO_0000020680Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi45 ↔ 64By similarity
Disulfide bondi66 ↔ 75By similarity
Disulfide bondi69 ↔ 82By similarity
Glycosylationi171 – 1711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence Analysis
Modified residuei276 – 2761N6-(pyridoxal phosphate)lysineBy similarity
Glycosylationi353 – 3531N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi393 ↔ 401By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

High expression in bulbs, lower expression in leaves, and no expression in roots.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ41233.
SMRiQ41233. Positions 26-452.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 8447EGF-like; atypicalAdd
BLAST

Domaini

The 6 Cys residues of the EGF-like domain are arranged in a disulfide pattern different from the one found in the canonical EGFs. The function of this domain is unclear. It may be a binding site for other proteins or the docking site for a putative alliinase receptor (By similarity).By similarity

Sequence similaritiesi

Belongs to the alliinase family.Curated
Contains 1 EGF-like domain.Curated

Keywords - Domaini

EGF-like domain, Signal

Family and domain databases

Gene3Di2.10.25.30. 1 hit.
3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR006948. Alliinase_C.
IPR006947. EGF_alliinase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF04864. Alliinase_C. 1 hit.
PF04863. EGF_alliinase. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q41233-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MICLVILTCI IMSNSFVNNN NMVQAKMTWT MKAAEEAEAV ANINCSEHGR
60 70 80 90 100
AFLDGIISEG SPKCECNTCY TGPDCSEKIQ GCSADVASGD GLFLEEYWKQ
110 120 130 140 150
HKEASAVLVS PWHRMSYFFN PVSNFISFEL EKTIKELHEV VGNAAAKDRY
160 170 180 190 200
IVFGVGVTQL IHGLVISLSP NMTATPDAPE SKVVAHAPFY PVFREQTKYF
210 220 230 240 250
DKKGYVWAGN AANYVNVSNP EQYIEMVTSP NNPEGLLRHA VIKGCKSIYD
260 270 280 290 300
MVYYWPHYTP IKYKADEDIL LFTMSKFTGH SGSRFGWALI KDESVYNNLL
310 320 330 340 350
NYMTKNTEGT PRETQLRSLK VLKEIVAMVK TQKGTMRDLN TFGFKKLRER
360 370 380 390 400
WVNITALLDQ SDRFSYQELP QSEYCNYFRR MRPPSPSYAW VNCEWEEDKD
410 420 430 440 450
CYQTFQNGRI NTQSGVGFEA SSRYVRLSLI KTQDDFDQLM YYLKDMVKAK
460 470
RKTPLIKQLF TDETETASRR PFI
Length:473
Mass (Da):54,183
Last modified:November 1, 1996 - v1
Checksum:i19F08669BA95A047
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S73324 mRNA. Translation: AAB32477.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

What's that smell? - Issue 39 of October 2003

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S73324 mRNA. Translation: AAB32477.1 .

3D structure databases

ProteinModelPortali Q41233.
SMRi Q41233. Positions 26-452.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 843. All s Alliin lyase.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-13491.

Family and domain databases

Gene3Di 2.10.25.30. 1 hit.
3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR006948. Alliinase_C.
IPR006947. EGF_alliinase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF04864. Alliinase_C. 1 hit.
PF04863. EGF_alliinase. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00022. EGF_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Alliin lyase (Alliinase) from garlic (Allium sativum). Biochemical characterization and cDNA cloning."
    Rabinkov A., Zhu X.Z., Grafi G., Galili G., Mirelman D.
    Appl. Biochem. Biotechnol. 48:149-171(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
    Tissue: Bulb.

Entry informationi

Entry nameiALLN2_ALLSA
AccessioniPrimary (citable) accession number: Q41233
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3