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Protein

Alliin lyase 2

Gene
N/A
Organism
Allium sativum (Garlic)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Able to cleave the C-S bond of sulfoxide derivatives of Cys to produce allicin, thus giving rise to all sulfur compounds which are responsible for most of the properties of garlic, such as the specific smell and flavor as well as the health benefits like blood lipid or blood pressure lowering.

Catalytic activityi

An S-alkyl-L-cysteine S-oxide = an alkyl sulfenate + 2-aminoacrylate.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei119ChlorideBy similarity1
Binding sitei123ChlorideBy similarity1
Binding sitei125ChlorideBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Chloride, Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13491.
BRENDAi4.4.1.4. 252.

Names & Taxonomyi

Protein namesi
Recommended name:
Alliin lyase 2 (EC:4.4.1.4)
Short name:
Alliinase-2
Alternative name(s):
Cysteine sulphoxide lyase 2
OrganismiAllium sativum (Garlic)
Taxonomic identifieri4682 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesAmaryllidaceaeAllioideaeAllieaeAllium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Protein family/group databases

Allergomei843. All s Alliin lyase.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Sequence analysisAdd BLAST15
PropeptideiPRO_000002067916 – 25By similarity10
ChainiPRO_000002068026 – 473Alliin lyase 2Add BLAST448

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi44N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi45 ↔ 64By similarity
Disulfide bondi66 ↔ 75By similarity
Disulfide bondi69 ↔ 82By similarity
Glycosylationi171N-linked (GlcNAc...)Sequence analysis1
Glycosylationi216N-linked (GlcNAc...)Sequence analysis1
Modified residuei276N6-(pyridoxal phosphate)lysineBy similarity1
Glycosylationi353N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi393 ↔ 401By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

High expression in bulbs, lower expression in leaves, and no expression in roots.

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ41233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 84EGF-like; atypicalAdd BLAST47

Domaini

The 6 Cys residues of the EGF-like domain are arranged in a disulfide pattern different from the one found in the canonical EGFs. The function of this domain is unclear. It may be a binding site for other proteins or the docking site for a putative alliinase receptor (By similarity).By similarity

Sequence similaritiesi

Belongs to the alliinase family.Curated
Contains 1 EGF-like domain.Curated

Keywords - Domaini

EGF-like domain, Signal

Family and domain databases

Gene3Di2.10.25.30. 1 hit.
3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR006948. Alliinase_C.
IPR006947. EGF_alliinase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF04864. Alliinase_C. 1 hit.
PF04863. EGF_alliinase. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q41233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MICLVILTCI IMSNSFVNNN NMVQAKMTWT MKAAEEAEAV ANINCSEHGR
60 70 80 90 100
AFLDGIISEG SPKCECNTCY TGPDCSEKIQ GCSADVASGD GLFLEEYWKQ
110 120 130 140 150
HKEASAVLVS PWHRMSYFFN PVSNFISFEL EKTIKELHEV VGNAAAKDRY
160 170 180 190 200
IVFGVGVTQL IHGLVISLSP NMTATPDAPE SKVVAHAPFY PVFREQTKYF
210 220 230 240 250
DKKGYVWAGN AANYVNVSNP EQYIEMVTSP NNPEGLLRHA VIKGCKSIYD
260 270 280 290 300
MVYYWPHYTP IKYKADEDIL LFTMSKFTGH SGSRFGWALI KDESVYNNLL
310 320 330 340 350
NYMTKNTEGT PRETQLRSLK VLKEIVAMVK TQKGTMRDLN TFGFKKLRER
360 370 380 390 400
WVNITALLDQ SDRFSYQELP QSEYCNYFRR MRPPSPSYAW VNCEWEEDKD
410 420 430 440 450
CYQTFQNGRI NTQSGVGFEA SSRYVRLSLI KTQDDFDQLM YYLKDMVKAK
460 470
RKTPLIKQLF TDETETASRR PFI
Length:473
Mass (Da):54,183
Last modified:November 1, 1996 - v1
Checksum:i19F08669BA95A047
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S73324 mRNA. Translation: AAB32477.1.

Cross-referencesi

Web resourcesi

Protein Spotlight

What's that smell? - Issue 39 of October 2003

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S73324 mRNA. Translation: AAB32477.1.

3D structure databases

ProteinModelPortaliQ41233.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei843. All s Alliin lyase.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13491.
BRENDAi4.4.1.4. 252.

Family and domain databases

Gene3Di2.10.25.30. 1 hit.
3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR006948. Alliinase_C.
IPR006947. EGF_alliinase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF04864. Alliinase_C. 1 hit.
PF04863. EGF_alliinase. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALLN2_ALLSA
AccessioniPrimary (citable) accession number: Q41233
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2003
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.