Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q41233

- ALLN2_ALLSA

UniProt

Q41233 - ALLN2_ALLSA

Protein

Alliin lyase 2

Gene
N/A
Organism
Allium sativum (Garlic)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Able to cleave the C-S bond of sulfoxide derivatives of Cys to produce allicin, thus giving rise to all sulfur compounds which are responsible for most of the properties of garlic, such as the specific smell and flavor as well as the health benefits like blood lipid or blood pressure lowering.

    Catalytic activityi

    An S-alkyl-L-cysteine S-oxide = an alkyl sulfenate + 2-aminoacrylate.

    Cofactori

    Pyridoxal phosphate.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei119 – 1191ChlorideBy similarity
    Binding sitei123 – 1231ChlorideBy similarity
    Binding sitei125 – 1251ChlorideBy similarity

    GO - Molecular functioni

    1. alliin lyase activity Source: UniProtKB-EC
    2. pyridoxal phosphate binding Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Chloride, Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13491.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alliin lyase 2 (EC:4.4.1.4)
    Short name:
    Alliinase-2
    Alternative name(s):
    Cysteine sulphoxide lyase 2
    OrganismiAllium sativum (Garlic)
    Taxonomic identifieri4682 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaAsparagalesAmaryllidaceaeAllioideaeAllieaeAllium

    Subcellular locationi

    GO - Cellular componenti

    1. vacuole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Vacuole

    Pathology & Biotechi

    Protein family/group databases

    Allergomei843. All s Alliin lyase.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1515Sequence AnalysisAdd
    BLAST
    Propeptidei16 – 2510By similarityPRO_0000020679
    Chaini26 – 473448Alliin lyase 2PRO_0000020680Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi44 – 441N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi45 ↔ 64By similarity
    Disulfide bondi66 ↔ 75By similarity
    Disulfide bondi69 ↔ 82By similarity
    Glycosylationi171 – 1711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi216 – 2161N-linked (GlcNAc...)Sequence Analysis
    Modified residuei276 – 2761N6-(pyridoxal phosphate)lysineBy similarity
    Glycosylationi353 – 3531N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi393 ↔ 401By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    High expression in bulbs, lower expression in leaves, and no expression in roots.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ41233.
    SMRiQ41233. Positions 26-452.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini38 – 8447EGF-like; atypicalAdd
    BLAST

    Domaini

    The 6 Cys residues of the EGF-like domain are arranged in a disulfide pattern different from the one found in the canonical EGFs. The function of this domain is unclear. It may be a binding site for other proteins or the docking site for a putative alliinase receptor By similarity.By similarity

    Sequence similaritiesi

    Belongs to the alliinase family.Curated
    Contains 1 EGF-like domain.Curated

    Keywords - Domaini

    EGF-like domain, Signal

    Family and domain databases

    Gene3Di2.10.25.30. 1 hit.
    3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR006948. Alliinase_C.
    IPR006947. EGF_alliinase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF04864. Alliinase_C. 1 hit.
    PF04863. EGF_alliinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00022. EGF_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q41233-1 [UniParc]FASTAAdd to Basket

    « Hide

    MICLVILTCI IMSNSFVNNN NMVQAKMTWT MKAAEEAEAV ANINCSEHGR    50
    AFLDGIISEG SPKCECNTCY TGPDCSEKIQ GCSADVASGD GLFLEEYWKQ 100
    HKEASAVLVS PWHRMSYFFN PVSNFISFEL EKTIKELHEV VGNAAAKDRY 150
    IVFGVGVTQL IHGLVISLSP NMTATPDAPE SKVVAHAPFY PVFREQTKYF 200
    DKKGYVWAGN AANYVNVSNP EQYIEMVTSP NNPEGLLRHA VIKGCKSIYD 250
    MVYYWPHYTP IKYKADEDIL LFTMSKFTGH SGSRFGWALI KDESVYNNLL 300
    NYMTKNTEGT PRETQLRSLK VLKEIVAMVK TQKGTMRDLN TFGFKKLRER 350
    WVNITALLDQ SDRFSYQELP QSEYCNYFRR MRPPSPSYAW VNCEWEEDKD 400
    CYQTFQNGRI NTQSGVGFEA SSRYVRLSLI KTQDDFDQLM YYLKDMVKAK 450
    RKTPLIKQLF TDETETASRR PFI 473
    Length:473
    Mass (Da):54,183
    Last modified:November 1, 1996 - v1
    Checksum:i19F08669BA95A047
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S73324 mRNA. Translation: AAB32477.1.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    What's that smell? - Issue 39 of October 2003

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S73324 mRNA. Translation: AAB32477.1 .

    3D structure databases

    ProteinModelPortali Q41233.
    SMRi Q41233. Positions 26-452.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 843. All s Alliin lyase.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-13491.

    Family and domain databases

    Gene3Di 2.10.25.30. 1 hit.
    3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR006948. Alliinase_C.
    IPR006947. EGF_alliinase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF04864. Alliinase_C. 1 hit.
    PF04863. EGF_alliinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00022. EGF_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Alliin lyase (Alliinase) from garlic (Allium sativum). Biochemical characterization and cDNA cloning."
      Rabinkov A., Zhu X.Z., Grafi G., Galili G., Mirelman D.
      Appl. Biochem. Biotechnol. 48:149-171(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
      Tissue: Bulb.

    Entry informationi

    Entry nameiALLN2_ALLSA
    AccessioniPrimary (citable) accession number: Q41233
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 3, 2003
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3