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Protein

Cold shock protein 2

Gene

CSP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Chaperone that binds to RNA, single- (ssDNA) and double-stranded (dsDNA) DNA, and unwinds nucleic acid duplex. Accelerates seed germination and seedling growth under cold stress, and contributes to enhancement of cold and freezing tolerance. Regulates flowering transition, and flower and seed development. Promotes seed germination under salt stress. May regulate respiratory oxygen uptake.6 Publications

Caution

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri129 – 146CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri183 – 200CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

  • double-stranded DNA binding Source: UniProtKB
  • mRNA binding Source: UniProtKB
  • nucleic acid binding Source: TAIR
  • single-stranded DNA binding Source: TAIR
  • zinc ion binding Source: InterPro

GO - Biological processi

  • cold acclimation Source: UniProtKB
  • DNA duplex unwinding Source: TAIR
  • regulation of cellular respiration Source: UniProtKB
  • regulation of transcription, DNA-templated Source: InterPro
  • response to abscisic acid Source: UniProtKB
  • response to cold Source: UniProtKB
  • response to desiccation Source: UniProtKB
  • response to water deprivation Source: UniProtKB
  • seed development Source: TAIR
  • stamen development Source: TAIR
  • vegetative to reproductive phase transition of meristem Source: TAIR

Keywordsi

Molecular functionChaperone, DNA-binding, RNA-binding
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-72163 mRNA Splicing - Major Pathway
R-ATH-72165 mRNA Splicing - Minor Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Cold shock protein 2
Short name:
AtCSP2
Alternative name(s):
Cold shock domain-containing protein 2
Glycine-rich protein 2
Short name:
AtGRP2
Gene namesi
Name:CSP2
Synonyms:CSDP2, GRP2
Ordered Locus Names:At4g38680
ORF Names:F20M13.240
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G38680
TAIRilocus:2121219 AT4G38680

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Early flowering, altered stamen number and impaired seed development.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004181592 – 203Cold shock protein 2Add BLAST202

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ41188
PRIDEiQ41188

PTM databases

iPTMnetiQ41188

Expressioni

Tissue specificityi

Mostly expressed in shoot apices, seeds and siliques, and, to a lower extent, in roots, cotyledons, stems, shoots, leaves, floral buds and flowers. Present in shoot apical meristems and siliques (at protein level).4 Publications

Developmental stagei

Active in meristematic tissues. During vegetative growth, expressed in root and shoot apical regions, leaves and roots primordia, root vascular cylinder and procambium. In reproductive organs, present in developing floral meristem, meristematic tissues of young flower buds, and placentae, pollen, ovules and seeds, mostly in embryos. High expression in the earliest stage of silique development, with a decrease during the middle stages of silique development and subsequently an increase during the later stages. In mature seeds, mostly localized in hypocotyl and radicule. During seed germination, first observed in the tip of the radicule and, upon opening of the cotyledons, expressed in the radicule and hypocotyl/radicule transition zone.3 Publications

Inductioni

Induced by abscisic acid (ABA), transiently by ethylene, salicylic acid and water fload, but down-regulated by dehydration stress. Accumulates during cold acclimation. Accumulation during silique development is AGL15-dependent.5 Publications

Gene expression databases

ExpressionAtlasiQ41188 baseline and differential
GenevisibleiQ41188 AT

Interactioni

Protein-protein interaction databases

BioGridi153041 interactor.
IntActiQ41188 4 interactors.
STRINGi3702.AT4G38680.1

Structurei

3D structure databases

ProteinModelPortaliQ41188
SMRiQ41188
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 76CSDAdd BLAST65

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi76 – 202Gly-richAdd BLAST127

Sequence similaritiesi

Belongs to the cold shock protein (CSP) family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri129 – 146CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri183 – 200CCHC-type 2PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG3070 Eukaryota
COG1278 LUCA
HOGENOMiHOG000186262
InParanoidiQ41188
OMAiARDCPTN
OrthoDBiEOG09360SVB
PhylomeDBiQ41188

Family and domain databases

CDDicd04458 CSP_CDS, 1 hit
InterProiView protein in InterPro
IPR019844 Cold-shock_CS
IPR011129 CSD
IPR002059 CSP_DNA-bd
IPR012340 NA-bd_OB-fold
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF00313 CSD, 1 hit
PF00098 zf-CCHC, 2 hits
PRINTSiPR00050 COLDSHOCK
SMARTiView protein in SMART
SM00357 CSP, 1 hit
SM00343 ZnF_C2HC, 2 hits
SUPFAMiSSF50249 SSF50249, 1 hit
SSF57756 SSF57756, 2 hits
PROSITEiView protein in PROSITE
PS00352 CSD_1, 1 hit
PS51857 CSD_2, 1 hit
PS50158 ZF_CCHC, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q41188-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGDNGGGER RKGSVKWFDT QKGFGFITPD DGGDDLFVHQ SSIRSEGFRS
60 70 80 90 100
LAAEEAVEFE VEIDNNNRPK AIDVSGPDGA PVQGNSGGGS SGGRGGFGGG
110 120 130 140 150
RGGGRGSGGG YGGGGGGYGG RGGGGRGGSD CYKCGEPGHM ARDCSEGGGG
160 170 180 190 200
YGGGGGGYGG GGGYGGGGGG YGGGGRGGGG GGGSCYSCGE SGHFARDCTS

GGR
Length:203
Mass (Da):19,153
Last modified:November 1, 1996 - v1
Checksum:i9310C89C1BC44F55
GO
Isoform 2 (identifier: Q41188-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     177-203: GGGGGGGSCYSCGESGHFARDCTSGGR → LLSLYYPLFAYYDGSLSLLVGFFLMVSD

Note: No experimental confirmation available.
Show »
Length:204
Mass (Da):19,881
Checksum:i0924242CFB0BD144
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044000177 – 203GGGGG…TSGGR → LLSLYYPLFAYYDGSLSLLV GFFLMVSD in isoform 2. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S47408 mRNA Translation: AAB24074.1
AL035540 Genomic DNA Translation: CAB37524.1
AL161593 Genomic DNA Translation: CAB80532.1
CP002687 Genomic DNA Translation: AEE86964.1
AF360202 mRNA Translation: AAK25912.1
AF385696 mRNA Translation: AAK60289.1
AY040049 mRNA Translation: AAK64107.1
AY133591 mRNA Translation: AAM91421.1
AK318762 mRNA Translation: BAH56877.1
PIRiJQ1061
RefSeqiNP_195580.1, NM_120029.3 [Q41188-1]
UniGeneiAt.24348
At.75605

Genome annotation databases

EnsemblPlantsiAT4G38680.1; AT4G38680.1; AT4G38680 [Q41188-1]
GeneIDi830024
GrameneiAT4G38680.1; AT4G38680.1; AT4G38680 [Q41188-1]
KEGGiath:AT4G38680

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCSP2_ARATH
AccessioniPrimary (citable) accession number: Q41188
Secondary accession number(s): C0Z2E8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2012
Last sequence update: November 1, 1996
Last modified: April 25, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome