ID   COMT2_POPTM             Reviewed;         364 AA.
AC   Q41086;
DT   11-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   20-JAN-2009, entry version 52.
DE   RecName: Full=Caffeic acid 3-O-methyltransferase 2;
DE            EC=2.1.1.68;
DE   AltName: Full=S-adenosysl-L-methionine:caffeic acid 3-O-methyltransferase 2;
DE            Short=CAOMT-2;
DE            Short=COMT-2;
GN   Name=OMT2;
OS   Populus tremuloides (Quaking aspen).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Malpighiales; Salicaceae; Saliceae; Populus.
OX   NCBI_TaxID=3693;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Hu W.-J., Chiang V.L.C.;
RT   "Nucleotide sequence of an additional member of bispecific caffeic
RT   acid/5-hydroxyferulic acid O-methyltransferase gene family in Populus
RT   tremuloides.";
RL   (er) Plant Gene Register PGR97-035.
CC   -!- FUNCTION: Catalyzes the conversion of caffeic acid to ferulic acid
CC       and of 5-hydroxyferulic acid to sinapic acid. The resulting
CC       products may subsequently be converted to the corresponding
CC       alcohols that are incorporated into lignins.
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + 3,4-dihydroxy-trans-
CC       cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-
CC       cinnamate.
CC   -!- PATHWAY: Aromatic compound metabolism; phenylpropanoid
CC       biosynthesis.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. Type 2
CC       family. COMT subfamily.
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DR   EMBL; U50522; AAB68049.1; -; Genomic_DNA.
DR   PIR; T09780; T09780.
DR   HSSP; P28002; 1KYZ.
DR   SMR; Q41086; 5-363.
DR   BRENDA; 2.1.1.68; 262298.
DR   GO; GO:0047763; F:caffeate O-methyltransferase activity; IEA:EC.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   GO; GO:0009809; P:lignin biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR016461; O-MeTrfase_COMT_euk.
DR   InterPro; IPR001077; O_MeTrfase_2.
DR   InterPro; IPR012967; Plant_MeTrfase_dimerisation.
DR   InterPro; IPR011991; Wing_hlx_DNA_bd.
DR   Gene3D; G3DSA:1.10.10.10; Wing_hlx_DNA_bd; 1.
DR   Pfam; PF08100; Dimerisation; 1.
DR   Pfam; PF00891; Methyltransf_2; 1.
DR   PIRSF; PIRSF005739; O-mtase; 1.
PE   3: Inferred from homology;
KW   Lignin biosynthesis; Methyltransferase; S-adenosyl-L-methionine;
KW   Transferase.
FT   CHAIN         1    364       Caffeic acid 3-O-methyltransferase 2.
FT                                /FTId=PRO_0000063210.
FT   REGION      129    135       Substrate binding (By similarity).
FT   REGION      161    179       Substrate binding (By similarity).
FT   ACT_SITE    268    268       Proton acceptor (By similarity).
FT   BINDING     207    207       S-adenosyl-L-methionine; via carbonyl
FT                                oxygen (By similarity).
FT   BINDING     230    230       S-adenosyl-L-methionine (By similarity).
FT   BINDING     250    250       S-adenosyl-L-methionine (By similarity).
FT   BINDING     251    251       S-adenosyl-L-methionine; via amide
FT                                nitrogen (By similarity).
FT   BINDING     264    264       S-adenosyl-L-methionine (By similarity).
SQ   SEQUENCE   364 AA;  39658 MW;  078C0DE060C1B65F CRC64;
     MGSTGETQMS PAQILDEEAN FALQLISSSV LPMVLKTAIE LDLLEIMAKA GPGALLPPSD
     IASHLPTKNP NAPVMLDRIL RLLASYSILI CSLRDLPDGK VERLYGLASV CKFLTRNEDG
     VSVSPLCLMN QDKVLMESWY HLKDAILEGG IPFNKAYGMT AFEYHGTDPR FNKVFNKGMS
     VHSKMAMKKI LETYKGFEGL ASLVDVGGGT GAVVSTIVSK YPSIKGINFD LPHVIADAPA
     FPGVENVGGD MFVSVPKADA VFMKWICHDW SDEHCLTFLK NCYDALPENG KVILVECILP
     VAPDTSLATK GVMHVDVIML AHNPGGKERT DREFESLARG AGFKGFEVMC CAFNTHVIEF
     RKKA
//