Reviewed, UniProtKB/Swiss-Prot Q41086 (COMT2_POPTM)

Names and origin

Protein names

Recommended name:
    Caffeic acid 3-O-methyltransferase 2
    EC=2.1.1.68
Alternative name(s):
    S-adenosysl-L-methionine:caffeic acid 3-O-methyltransferase 2
      Short name=CAOMT-2
      Short name=COMT-2

Gene names

Name:

OMT2

Organism

Populus tremuloides (Quaking aspen)

Taxonomic identifier

3693 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the conversion of caffeic acid to ferulic acid and of 5-hydroxyferulic acid to sinapic acid. The resulting products may subsequently be converted to the corresponding alcohols that are incorporated into lignins.
Catalytic activity	S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate.
Pathway	Aromatic compound metabolism; phenylpropanoid biosynthesis.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. Type 2 family. COMT subfamily.

Ontologies

Keywords
Biological process	Lignin biosynthesis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	lignin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	caffeate O-methyltransferase activity Inferred from electronic annotation. Source: EC protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Caffeic acid 3-O-methyltransferase 2		PRO_0000063210
Regions
	Region	129 – 135	7	Substrate binding By similarity
	Region	161 – 179	19	Substrate binding By similarity
Sites
	Active site	268	1	Proton acceptor By similarity
	Binding site	207	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	230	1	S-adenosyl-L-methionine By similarity
	Binding site	250	1	S-adenosyl-L-methionine By similarity
	Binding site	251	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	264	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q41086-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 078C0DE060C1B65F	FASTA	364	39,658
	10 20 30 40 50 60 MGSTGETQMS PAQILDEEAN FALQLISSSV LPMVLKTAIE LDLLEIMAKA GPGALLPPSD 70 80 90 100 110 120 IASHLPTKNP NAPVMLDRIL RLLASYSILI CSLRDLPDGK VERLYGLASV CKFLTRNEDG 130 140 150 160 170 180 VSVSPLCLMN QDKVLMESWY HLKDAILEGG IPFNKAYGMT AFEYHGTDPR FNKVFNKGMS 190 200 210 220 230 240 VHSKMAMKKI LETYKGFEGL ASLVDVGGGT GAVVSTIVSK YPSIKGINFD LPHVIADAPA 250 260 270 280 290 300 FPGVENVGGD MFVSVPKADA VFMKWICHDW SDEHCLTFLK NCYDALPENG KVILVECILP 310 320 330 340 350 360 VAPDTSLATK GVMHVDVIML AHNPGGKERT DREFESLARG AGFKGFEVMC CAFNTHVIEF RKKA

References

[1]	"Nucleotide sequence of an additional member of bispecific caffeic acid/5-hydroxyferulic acid O-methyltransferase gene family in Populus tremuloides." Hu W.-J., Chiang V.L.C. Plant Gene Register PGR97-035 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U50522 Genomic DNA. Translation: AAB68049.1.
PIR	T09780.
3D structure databases
HSSP	HSSP built from PDB template 1KYZ based on UniProtKB P28002.
SMR	Q41086. Positions 5-363.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.68. 262298.
Family and domain databases
InterPro	IPR016461. O-MeTrfase_COMT_euk. IPR001077. O_MeTrfase_2. IPR012967. Plant_MeTrfase_dimerisation. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF08100. Dimerisation. 1 hit. PF00891. Methyltransf_2. 1 hit. [Graphical view]
PIRSF	PIRSF005739. O-mtase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COMT2_POPTM

Accession

Primary (citable) accession number: Q41086

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	November 1, 1996
Last modified:	January 20, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)