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Protein

1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic

Gene

SBEII

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. May preferentially transfer long chains during branching.1 Publication

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathway:istarch biosynthesis

This protein is involved in the pathway starch biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway starch biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei409 – 4091NucleophileBy similarity
Active sitei464 – 4641Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

UniPathwayiUPA00152.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic (EC:2.4.1.18)
Alternative name(s):
Starch branching enzyme I
Gene namesi
Name:SBEII
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei‹1 – 58›58ChloroplastAdd
BLAST
Chaini59 – 8267681,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplasticSequence AnalysisPRO_5000146302Add
BLAST

Expressioni

Tissue specificityi

Expressed in roots, leaves, stipules, pods and flowers.1 Publication

Developmental stagei

Very lov expression in young embryos, increasing during maturation.1 Publication

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ41059.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q41059-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
ATTTTTTHNS KNKQYLAKQK PVELTLGYQN PNGCKVCSFG SKGSIYQKVS
60 70 80 90 100
SGFKGVSVMT DDKSTMPSVE EDFENIGILN VDSSLEPFKD HFKYRLKRYL
110 120 130 140 150
HQKKLIEEYE GGLQEFAKGY LKFGFNREED GISYREWAPA AQEAQIIGDF
160 170 180 190 200
NGWNGSNLHM EKDQFGVWSI QIPDADGNPA IPHNSRVKFR FKHSDGVWVD
210 220 230 240 250
RIPAWIKYAT VDPTRFAAPY DGVYWDPPLS ERYQFKHPRP PKPKAPRIYE
260 270 280 290 300
AHVGMSSSEP RINSYREFAD DVLPRIRENN YNTVQLMAVM EHSYYASFWY
310 320 330 340 350
HVTKPFFAVS SRSGSPEDLK YLIDKAHSLG LNVLMDVIHS HASNNVTDGL
360 370 380 390 400
NGFDVGQSSQ QSYFHAGDRG YHKLWDSRLF NYANWKSSFL LSNLRWWLEE
410 420 430 440 450
YKFDGFRFDG VTSMLYHHHG INMAFTGDYN EYFSEETDVD AVVYLMLANS
460 470 480 490 500
LVHDILPDAT DIAEDVSGMP GLGRPVSEVG IGFDYRLAMA IPDKWIDYLK
510 520 530 540 550
NKKDSEWSMK EISLNLTNRR YTEKCVSYAE SHDQSIVGDK TIAFLLMDEE
560 570 580 590 600
MYSSMSCLTM LSPTIERGIS LHKMIHFITL ALGGEGYLNF MGNEFGHPEW
610 620 630 640 650
IDFPREGNGW SYEKCRLTQW NLVDTNHLRY KFMNAFDRAM NLLDDKFSIL
660 670 680 690 700
ASTKQIVSST NNEDKVIVFE RGDLVFVFNF HPENTYEGYK VGCDLPGKYR
710 720 730 740 750
VALDSDATEF GGHGRVGHDA DQFTSPEGIP GIPETNFNNR PNSFKVLSPP
760 770 780 790 800
HTCVVYYRVD ERQEESNNPN LGSVEETFAA ADTDVARIPD VSMESEDSNL
810 820
DRIEDNSEDA VDAGILKVER EVVGDN
Length:826
Mass (Da):94,191
Last modified:November 1, 1996 - v1
Checksum:i46B9D1965CB5EC9C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80010 mRNA. Translation: CAA56320.1.
PIRiT06494.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80010 mRNA. Translation: CAA56320.1.
PIRiT06494.

3D structure databases

ProteinModelPortaliQ41059.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00152.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Starch branching enzymes belonging to distinct enzyme families are differentially expressed during pea embryo development."
    Burton R.A., Bewley J.D., Smith A.M., Bhattacharyya M.K., Tatge H., Ring S., Bull V., Hamilton W.D., Martin C.
    Plant J. 7:3-15(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 58-72, FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiGLGB2_PEA
AccessioniPrimary (citable) accession number: Q41059
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: November 1, 1996
Last modified: January 7, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.