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Q41059

- GLGB2_PEA

UniProt

Q41059 - GLGB2_PEA

Protein

1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic

Gene

SBEII

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. May preferentially transfer long chains during branching.1 Publication

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei409 – 4091NucleophileBy similarity
    Active sitei464 – 4641Proton donorBy similarity

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-EC
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: InterPro
    2. starch biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00152.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic (EC:2.4.1.18)
    Alternative name(s):
    Starch branching enzyme I
    Gene namesi
    Name:SBEII
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    Subcellular locationi

    GO - Cellular componenti

    1. amyloplast Source: UniProtKB-SubCell
    2. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Amyloplast, Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei‹1 – 58›58ChloroplastAdd
    BLAST
    Chaini59 – 8267681,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplasticSequence AnalysisPRO_5000146302Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves, stipules, pods and flowers.1 Publication

    Developmental stagei

    Very lov expression in young embryos, increasing during maturation.1 Publication

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ41059.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q41059-1 [UniParc]FASTAAdd to Basket

    « Hide

    ATTTTTTHNS KNKQYLAKQK PVELTLGYQN PNGCKVCSFG SKGSIYQKVS    50
    SGFKGVSVMT DDKSTMPSVE EDFENIGILN VDSSLEPFKD HFKYRLKRYL 100
    HQKKLIEEYE GGLQEFAKGY LKFGFNREED GISYREWAPA AQEAQIIGDF 150
    NGWNGSNLHM EKDQFGVWSI QIPDADGNPA IPHNSRVKFR FKHSDGVWVD 200
    RIPAWIKYAT VDPTRFAAPY DGVYWDPPLS ERYQFKHPRP PKPKAPRIYE 250
    AHVGMSSSEP RINSYREFAD DVLPRIRENN YNTVQLMAVM EHSYYASFWY 300
    HVTKPFFAVS SRSGSPEDLK YLIDKAHSLG LNVLMDVIHS HASNNVTDGL 350
    NGFDVGQSSQ QSYFHAGDRG YHKLWDSRLF NYANWKSSFL LSNLRWWLEE 400
    YKFDGFRFDG VTSMLYHHHG INMAFTGDYN EYFSEETDVD AVVYLMLANS 450
    LVHDILPDAT DIAEDVSGMP GLGRPVSEVG IGFDYRLAMA IPDKWIDYLK 500
    NKKDSEWSMK EISLNLTNRR YTEKCVSYAE SHDQSIVGDK TIAFLLMDEE 550
    MYSSMSCLTM LSPTIERGIS LHKMIHFITL ALGGEGYLNF MGNEFGHPEW 600
    IDFPREGNGW SYEKCRLTQW NLVDTNHLRY KFMNAFDRAM NLLDDKFSIL 650
    ASTKQIVSST NNEDKVIVFE RGDLVFVFNF HPENTYEGYK VGCDLPGKYR 700
    VALDSDATEF GGHGRVGHDA DQFTSPEGIP GIPETNFNNR PNSFKVLSPP 750
    HTCVVYYRVD ERQEESNNPN LGSVEETFAA ADTDVARIPD VSMESEDSNL 800
    DRIEDNSEDA VDAGILKVER EVVGDN 826
    Length:826
    Mass (Da):94,191
    Last modified:November 1, 1996 - v1
    Checksum:i46B9D1965CB5EC9C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80010 mRNA. Translation: CAA56320.1.
    PIRiT06494.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80010 mRNA. Translation: CAA56320.1 .
    PIRi T06494.

    3D structure databases

    ProteinModelPortali Q41059.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00152 .

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Starch branching enzymes belonging to distinct enzyme families are differentially expressed during pea embryo development."
      Burton R.A., Bewley J.D., Smith A.M., Bhattacharyya M.K., Tatge H., Ring S., Bull V., Hamilton W.D., Martin C.
      Plant J. 7:3-15(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 58-72, FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiGLGB2_PEA
    AccessioniPrimary (citable) accession number: Q41059
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 19, 2014
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3