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Q41059 (GLGB2_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic

EC=2.4.1.18
Alternative name(s):
Starch branching enzyme I
Gene names
Name:SBEII
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length826 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. May preferentially transfer long chains during branching. Ref.1

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathway

Glycan biosynthesis; starch biosynthesis.

Subunit structure

Monomer By similarity.

Subcellular location

Plastidchloroplast. Plastidamyloplast Potential.

Tissue specificity

Expressed in roots, leaves, stipules, pods and flowers. Ref.1

Developmental stage

Very lov expression in young embryos, increasing during maturation. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide‹1 – 58›58Chloroplast
Chain59 – 8267681,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic Potential
PRO_5000146302

Sites

Active site4091Nucleophile By similarity
Active site4641Proton donor By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Q41059 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 46B9D1965CB5EC9C

FASTA82694,191
        10         20         30         40         50         60 
ATTTTTTHNS KNKQYLAKQK PVELTLGYQN PNGCKVCSFG SKGSIYQKVS SGFKGVSVMT 

        70         80         90        100        110        120 
DDKSTMPSVE EDFENIGILN VDSSLEPFKD HFKYRLKRYL HQKKLIEEYE GGLQEFAKGY 

       130        140        150        160        170        180 
LKFGFNREED GISYREWAPA AQEAQIIGDF NGWNGSNLHM EKDQFGVWSI QIPDADGNPA 

       190        200        210        220        230        240 
IPHNSRVKFR FKHSDGVWVD RIPAWIKYAT VDPTRFAAPY DGVYWDPPLS ERYQFKHPRP 

       250        260        270        280        290        300 
PKPKAPRIYE AHVGMSSSEP RINSYREFAD DVLPRIRENN YNTVQLMAVM EHSYYASFWY 

       310        320        330        340        350        360 
HVTKPFFAVS SRSGSPEDLK YLIDKAHSLG LNVLMDVIHS HASNNVTDGL NGFDVGQSSQ 

       370        380        390        400        410        420 
QSYFHAGDRG YHKLWDSRLF NYANWKSSFL LSNLRWWLEE YKFDGFRFDG VTSMLYHHHG 

       430        440        450        460        470        480 
INMAFTGDYN EYFSEETDVD AVVYLMLANS LVHDILPDAT DIAEDVSGMP GLGRPVSEVG 

       490        500        510        520        530        540 
IGFDYRLAMA IPDKWIDYLK NKKDSEWSMK EISLNLTNRR YTEKCVSYAE SHDQSIVGDK 

       550        560        570        580        590        600 
TIAFLLMDEE MYSSMSCLTM LSPTIERGIS LHKMIHFITL ALGGEGYLNF MGNEFGHPEW 

       610        620        630        640        650        660 
IDFPREGNGW SYEKCRLTQW NLVDTNHLRY KFMNAFDRAM NLLDDKFSIL ASTKQIVSST 

       670        680        690        700        710        720 
NNEDKVIVFE RGDLVFVFNF HPENTYEGYK VGCDLPGKYR VALDSDATEF GGHGRVGHDA 

       730        740        750        760        770        780 
DQFTSPEGIP GIPETNFNNR PNSFKVLSPP HTCVVYYRVD ERQEESNNPN LGSVEETFAA 

       790        800        810        820 
ADTDVARIPD VSMESEDSNL DRIEDNSEDA VDAGILKVER EVVGDN 

« Hide

References

[1]"Starch branching enzymes belonging to distinct enzyme families are differentially expressed during pea embryo development."
Burton R.A., Bewley J.D., Smith A.M., Bhattacharyya M.K., Tatge H., Ring S., Bull V., Hamilton W.D., Martin C.
Plant J. 7:3-15(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 58-72, FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X80010 mRNA. Translation: CAA56320.1.
PIRT06494.

3D structure databases

ProteinModelPortalQ41059.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00152.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB2_PEA
AccessionPrimary (citable) accession number: Q41059
Entry history
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries