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Q41058

- GLGB1_PEA

UniProt

Q41058 - GLGB1_PEA

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Protein

1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic

Gene

SBEI

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. May preferentially transfer short chains during branching. Responsible for the synthesis of about 75% of the amylopectin found in the starch granules of mature embryos.1 Publication

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei494 – 4941NucleophileBy similarity
Active sitei549 – 5491Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. embryo development Source: UniProtKB
  2. starch biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

UniPathwayiUPA00152.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic (EC:2.4.1.18)
Alternative name(s):
Starch branching enzyme I
Gene namesi
Name:SBEI
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

  1. amyloplast Source: UniProtKB-KW
  2. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Amyloplast, Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Wrinkled seeds.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4747Chloroplast1 PublicationAdd
BLAST
Chaini48 – 9228751,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplasticPRO_5000146301Add
BLAST

Expressioni

Tissue specificityi

Expressed in roots, leaves, stipules, pods and flowers.1 Publication

Developmental stagei

Highly expressed during early stages of embryo development. Decreasing expression during the embryo maturation.2 Publications

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ41058.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi849 – 91971Glu-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase_b_C.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q41058-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVYTISGIRF PVLPSLHKST LRCDRRASSH SFFLKNNSSS FSRTSLYAKF
60 70 80 90 100
SRDSETKSST IAESDKVLIP EDQDNSVSLA DQLENPDITS EDAQNLEDLT
110 120 130 140 150
MKDGNKYNID ESTSSYREVG DEKGSVTSSS LVDVNTDTQA KKTSVHSDKK
160 170 180 190 200
VKVDKPKIIP PPGTGQKIYE IDPLLQAHRQ HLDFRYGQYK RIREEIDKYE
210 220 230 240 250
GGLDAFSRGY EKFGFTRSAT GITYREWAPG AKSAALVGDF NNWNPNADVM
260 270 280 290 300
TKDAFGVWEI FLPNNADGSP PIPHGSRVKI HMDTPSGIKD SIPAWIKFSV
310 320 330 340 350
QAPGEIPYNG IYYDPPEEEK YVFKHPQPKR PQSIRIYESH IGMSSPEPKI
360 370 380 390 400
NTYANFRDDV LPRIKKLGYN AVQIMAIQEH SYYASFGYHV TNFFAPSSRF
410 420 430 440 450
GTPEDLKSLI DRAHELGLLV LMDIVHSHSS NNTLDGLNMF DGTDGHYFHP
460 470 480 490 500
GSRGYHWMWD SRLFNYGSWE VLRYLLSNAR WWLDEYKFDG FRFDGVTSMM
510 520 530 540 550
YTHHGLQVSF TGNYSEYFGL ATDVEAVVYM MLVNDLIHGL FPEAVSIGED
560 570 580 590 600
VSGMPTFCLP TQDGGIGFNY RLHMAVADKW IELLKKQDED WRMGDIVHTL
610 620 630 640 650
TNRRWLEKCV VYAESHDQAL VGDKTLAFWL MDKDMYDFMA LDRPSTPLID
660 670 680 690 700
RGIALHKMIR LITMGLGGEG YLNFMGNEFG HPEWIDFPRG EQHLPNGKIV
710 720 730 740 750
PGNNNSYDKC RRRFDLGDAD YLRYHGMQEF DRAMQHLEER YGFMTSEHQY
760 770 780 790 800
ISRKNEGDRV IIFERDNLVF VFNFHWTNSY SDYKVGCLKP GKYKIVLDSD
810 820 830 840 850
DTLFGGFNRL NHTAEYFTSE GWYDDRPRSF LVYAPSRTAV VYALADGVES
860 870 880 890 900
EPIELSDGVE SEPIELSVGV ESEPIELSVE EAESEPIERS VEEVESETTQ
910 920
QSVEVESETT QQSVEVESET TQ
Length:922
Mass (Da):105,227
Last modified:November 1, 1996 - v1
Checksum:i4EDBF9374C6385C8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80009 mRNA. Translation: CAA56319.1.
PIRiT06493.

Cross-referencesi

Web resourcesi

Protein Spotlight

On the garden pea - Issue 159 of April 2014

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X80009 mRNA. Translation: CAA56319.1 .
PIRi T06493.

3D structure databases

ProteinModelPortali Q41058.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00152 .

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProi IPR006048. A-amylase_b_C.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Starch branching enzymes belonging to distinct enzyme families are differentially expressed during pea embryo development."
    Burton R.A., Bewley J.D., Smith A.M., Bhattacharyya M.K., Tatge H., Ring S., Bull V., Hamilton W.D., Martin C.
    Plant J. 7:3-15(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 48-65, FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  2. "The wrinkled-seed character of pea described by Mendel is caused by a transposon-like insertion in a gene encoding starch-branching enzyme."
    Bhattacharyya M.K., Smith A.M., Ellis T.H., Hedley C., Martin C.
    Cell 60:115-122(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiGLGB1_PEA
AccessioniPrimary (citable) accession number: Q41058
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The wrinkled seeds phenotype (rr) studied by Mendel in 1866 is probably caused by an 800 bp insertion in the SBEI gene, leading to the loss of the last 61 amino acids of the protein and a complete absence of activity.1 Publication

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3