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Q41058 (GLGB1_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic

EC=2.4.1.18
Alternative name(s):
Starch branching enzyme I
Gene names
Name:SBEI
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Protein attributes

Sequence length922 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. May preferentially transfer short chains during branching. Responsible for the synthesis of about 75% of the amylopectin found in the starch granules of mature embryos. Ref.1

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. Ref.1

Pathway

Glycan biosynthesis; starch biosynthesis.

Subunit structure

Monomer By similarity.

Subcellular location

Plastidchloroplast. Plastidamyloplast Potential.

Tissue specificity

Expressed in roots, leaves, stipules, pods and flowers. Ref.1

Developmental stage

Highly expressed during early stages of embryo development. Decreasing expression during the embryo maturation. Ref.1 Ref.2

Disruption phenotype

Wrinkled seeds. Ref.2

Miscellaneous

The wrinkled seeds phenotype (rr) studied by Mendel in 1866 is probably caused by an 800 bp insertion in the SBEI gene, leading to the loss of the last 61 amino acids of the protein and a complete absence of activity (Ref.2).

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4747Chloroplast Ref.1
Chain48 – 9228751,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic
PRO_5000146301

Regions

Compositional bias849 – 91971Glu-rich

Sites

Active site4941Nucleophile By similarity
Active site5491Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q41058 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4EDBF9374C6385C8

FASTA922105,227
        10         20         30         40         50         60 
MVYTISGIRF PVLPSLHKST LRCDRRASSH SFFLKNNSSS FSRTSLYAKF SRDSETKSST 

        70         80         90        100        110        120 
IAESDKVLIP EDQDNSVSLA DQLENPDITS EDAQNLEDLT MKDGNKYNID ESTSSYREVG 

       130        140        150        160        170        180 
DEKGSVTSSS LVDVNTDTQA KKTSVHSDKK VKVDKPKIIP PPGTGQKIYE IDPLLQAHRQ 

       190        200        210        220        230        240 
HLDFRYGQYK RIREEIDKYE GGLDAFSRGY EKFGFTRSAT GITYREWAPG AKSAALVGDF 

       250        260        270        280        290        300 
NNWNPNADVM TKDAFGVWEI FLPNNADGSP PIPHGSRVKI HMDTPSGIKD SIPAWIKFSV 

       310        320        330        340        350        360 
QAPGEIPYNG IYYDPPEEEK YVFKHPQPKR PQSIRIYESH IGMSSPEPKI NTYANFRDDV 

       370        380        390        400        410        420 
LPRIKKLGYN AVQIMAIQEH SYYASFGYHV TNFFAPSSRF GTPEDLKSLI DRAHELGLLV 

       430        440        450        460        470        480 
LMDIVHSHSS NNTLDGLNMF DGTDGHYFHP GSRGYHWMWD SRLFNYGSWE VLRYLLSNAR 

       490        500        510        520        530        540 
WWLDEYKFDG FRFDGVTSMM YTHHGLQVSF TGNYSEYFGL ATDVEAVVYM MLVNDLIHGL 

       550        560        570        580        590        600 
FPEAVSIGED VSGMPTFCLP TQDGGIGFNY RLHMAVADKW IELLKKQDED WRMGDIVHTL 

       610        620        630        640        650        660 
TNRRWLEKCV VYAESHDQAL VGDKTLAFWL MDKDMYDFMA LDRPSTPLID RGIALHKMIR 

       670        680        690        700        710        720 
LITMGLGGEG YLNFMGNEFG HPEWIDFPRG EQHLPNGKIV PGNNNSYDKC RRRFDLGDAD 

       730        740        750        760        770        780 
YLRYHGMQEF DRAMQHLEER YGFMTSEHQY ISRKNEGDRV IIFERDNLVF VFNFHWTNSY 

       790        800        810        820        830        840 
SDYKVGCLKP GKYKIVLDSD DTLFGGFNRL NHTAEYFTSE GWYDDRPRSF LVYAPSRTAV 

       850        860        870        880        890        900 
VYALADGVES EPIELSDGVE SEPIELSVGV ESEPIELSVE EAESEPIERS VEEVESETTQ 

       910        920 
QSVEVESETT QQSVEVESET TQ 

« Hide

References

[1]"Starch branching enzymes belonging to distinct enzyme families are differentially expressed during pea embryo development."
Burton R.A., Bewley J.D., Smith A.M., Bhattacharyya M.K., Tatge H., Ring S., Bull V., Hamilton W.D., Martin C.
Plant J. 7:3-15(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 48-65, FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[2]"The wrinkled-seed character of pea described by Mendel is caused by a transposon-like insertion in a gene encoding starch-branching enzyme."
Bhattacharyya M.K., Smith A.M., Ellis T.H., Hedley C., Martin C.
Cell 60:115-122(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

Web resources

Protein Spotlight

On the garden pea - Issue 159 of April 2014

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X80009 mRNA. Translation: CAA56319.1.
PIRT06493.

3D structure databases

ProteinModelPortalQ41058.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00152.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR006048. A-amylase_b_C.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB1_PEA
AccessionPrimary (citable) accession number: Q41058
Entry history
Integrated into UniProtKB/Swiss-Prot: February 19, 2014
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries