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Q41058

- GLGB1_PEA

UniProt

Q41058 - GLGB1_PEA

Protein

1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic

Gene

SBEI

Organism
Pisum sativum (Garden pea)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in starch by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. May preferentially transfer short chains during branching. Responsible for the synthesis of about 75% of the amylopectin found in the starch granules of mature embryos.1 Publication

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei494 – 4941NucleophileBy similarity
    Active sitei549 – 5491Proton donorBy similarity

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. embryo development Source: UniProtKB
    2. starch biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00152.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplastic (EC:2.4.1.18)
    Alternative name(s):
    Starch branching enzyme I
    Gene namesi
    Name:SBEI
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

    Subcellular locationi

    GO - Cellular componenti

    1. amyloplast Source: UniProtKB-SubCell
    2. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Amyloplast, Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    Wrinkled seeds.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4747Chloroplast1 PublicationAdd
    BLAST
    Chaini48 – 9228751,4-alpha-glucan-branching enzyme 1, chloroplastic/amyloplasticPRO_5000146301Add
    BLAST

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves, stipules, pods and flowers.1 Publication

    Developmental stagei

    Highly expressed during early stages of embryo development. Decreasing expression during the embryo maturation.2 Publications

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ41058.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi849 – 91971Glu-richAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProiIPR006048. A-amylase_b_C.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q41058-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVYTISGIRF PVLPSLHKST LRCDRRASSH SFFLKNNSSS FSRTSLYAKF    50
    SRDSETKSST IAESDKVLIP EDQDNSVSLA DQLENPDITS EDAQNLEDLT 100
    MKDGNKYNID ESTSSYREVG DEKGSVTSSS LVDVNTDTQA KKTSVHSDKK 150
    VKVDKPKIIP PPGTGQKIYE IDPLLQAHRQ HLDFRYGQYK RIREEIDKYE 200
    GGLDAFSRGY EKFGFTRSAT GITYREWAPG AKSAALVGDF NNWNPNADVM 250
    TKDAFGVWEI FLPNNADGSP PIPHGSRVKI HMDTPSGIKD SIPAWIKFSV 300
    QAPGEIPYNG IYYDPPEEEK YVFKHPQPKR PQSIRIYESH IGMSSPEPKI 350
    NTYANFRDDV LPRIKKLGYN AVQIMAIQEH SYYASFGYHV TNFFAPSSRF 400
    GTPEDLKSLI DRAHELGLLV LMDIVHSHSS NNTLDGLNMF DGTDGHYFHP 450
    GSRGYHWMWD SRLFNYGSWE VLRYLLSNAR WWLDEYKFDG FRFDGVTSMM 500
    YTHHGLQVSF TGNYSEYFGL ATDVEAVVYM MLVNDLIHGL FPEAVSIGED 550
    VSGMPTFCLP TQDGGIGFNY RLHMAVADKW IELLKKQDED WRMGDIVHTL 600
    TNRRWLEKCV VYAESHDQAL VGDKTLAFWL MDKDMYDFMA LDRPSTPLID 650
    RGIALHKMIR LITMGLGGEG YLNFMGNEFG HPEWIDFPRG EQHLPNGKIV 700
    PGNNNSYDKC RRRFDLGDAD YLRYHGMQEF DRAMQHLEER YGFMTSEHQY 750
    ISRKNEGDRV IIFERDNLVF VFNFHWTNSY SDYKVGCLKP GKYKIVLDSD 800
    DTLFGGFNRL NHTAEYFTSE GWYDDRPRSF LVYAPSRTAV VYALADGVES 850
    EPIELSDGVE SEPIELSVGV ESEPIELSVE EAESEPIERS VEEVESETTQ 900
    QSVEVESETT QQSVEVESET TQ 922
    Length:922
    Mass (Da):105,227
    Last modified:November 1, 1996 - v1
    Checksum:i4EDBF9374C6385C8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80009 mRNA. Translation: CAA56319.1.
    PIRiT06493.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    On the garden pea - Issue 159 of April 2014

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X80009 mRNA. Translation: CAA56319.1 .
    PIRi T06493.

    3D structure databases

    ProteinModelPortali Q41058.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00152 .

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    InterProi IPR006048. A-amylase_b_C.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Starch branching enzymes belonging to distinct enzyme families are differentially expressed during pea embryo development."
      Burton R.A., Bewley J.D., Smith A.M., Bhattacharyya M.K., Tatge H., Ring S., Bull V., Hamilton W.D., Martin C.
      Plant J. 7:3-15(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 48-65, FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    2. "The wrinkled-seed character of pea described by Mendel is caused by a transposon-like insertion in a gene encoding starch-branching enzyme."
      Bhattacharyya M.K., Smith A.M., Ellis T.H., Hedley C., Martin C.
      Cell 60:115-122(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiGLGB1_PEA
    AccessioniPrimary (citable) accession number: Q41058
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 19, 2014
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The wrinkled seeds phenotype (rr) studied by Mendel in 1866 is probably caused by an 800 bp insertion in the SBEI gene, leading to the loss of the last 61 amino acids of the protein and a complete absence of activity.1 Publication

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3