Reviewed, UniProtKB/Swiss-Prot Q41014 (FENR2_PEA)

Names and origin

Protein names	Recommended name:     Ferredoxin--NADP reductase, root isozyme, chloroplastic       Short name=FNR     EC=1.18.1.2
Organism	Pisum sativum (Garden pea)
Taxonomic identifier	3888 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May play a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power. Is involved in nitrate assimilation.
Catalytic activity	2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH.
Cofactor	FAD.
Pathway	Energy metabolism; photosynthesis.
Subcellular location	Plastid › chloroplast By similarity.
Sequence similarities	Belongs to the ferredoxin--NADP reductase type 1 family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell thylakoid membrane Inferred from electronic annotation. Source: InterPro
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro ferredoxin-NADP+ reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 61	61	Chloroplast Potential
	Chain	62 – 377	316	Ferredoxin--NADP reductase, root isozyme, chloroplastic		PRO_0000019415
Regions
	Domain	92 – 220	129	FAD-binding FR-type
	Nucleotide binding	152 – 155	4	FAD By similarity
	Nucleotide binding	173 – 175	3	FAD By similarity
	Nucleotide binding	194 – 196	3	FAD By similarity
	Nucleotide binding	268 – 269	2	NADP By similarity
	Nucleotide binding	298 – 299	2	NADP By similarity
	Nucleotide binding	336 – 337	2	NADP By similarity
Sites
	Binding site	155	1	NADP By similarity
	Binding site	175	1	NADP By similarity
	Binding site	179	1	FAD By similarity
	Binding site	236	1	FAD By similarity
	Binding site	236	1	NADP; via amide nitrogen By similarity
	Binding site	308	1	NADP By similarity
	Binding site	375	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q41014-1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 6CAC68ECE5647D0A	FASTA	377	42,312
	10 20 30 40 50 60 MSHLAVSQMA VTVPVSSDFS VRRSAFKSSN LNFRDKSWAP VFTLGMKAKN CGWRNHNVIC 70 80 90 100 110 120 MSVQQASVPK VTVSPLELEN PSEPPLNLHK PKEPYTATIV SVERLVGPKA PGETCHIVIN 130 140 150 160 170 180 HDGNVPYWEG QSYGVIPPGE NPKKPGSPHN VRLYSIASTR YGDNFDGKTA SLCVRRAVYY 190 200 210 220 230 240 DPVTGKEDPS KNGVCSNFLC DSKPGDKIKI AGPSGKIMLL PEDDPNATHI MIATGTGVAP 250 260 270 280 290 300 YRGYLRRMFM ESVPTFKFGG LAWLFLGVAN VDSLLYDDEF TKYLKDYPDN FRYNRALSRE 310 320 330 340 350 360 EKNKNGGKMY VQDKIEEYSD EIFKLLDNGA HIYFCGLRGM MPGIQETLKR VAEKRGESWE 370 EKLSQLKKNK QWHVEVY

References

[1]	"The isolation of a pea root ferredoxin-NADP+ oxidoreductase (FNR) cDNA." Bowsher C.G., Knight J.S. Plant Gene Register PGR96-073 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Root.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X99419 mRNA. Translation: CAA67796.1. Different initiation.
PIR	T06773.
3D structure databases
HSSP	HSSP built from PDB template 1JB9 based on UniProtKB Q41736.
SMR	Q41014. Positions 67-377.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.18.1.2. 287.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR012146. Frd-NADP+_RD. IPR015701. FRD_Red. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
PANTHER	PTHR19384:SF1. FRD_Red. 1 hit.
Pfam	PF00175. NAD_binding_1. 1 hit. [Graphical view]
PIRSF	PIRSF000361. Frd-NADP+_RD. 1 hit.
PRINTS	PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FENR2_PEA

Accession

Primary (citable) accession number: Q41014

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 15, 1998
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)