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Protein

Translocase of chloroplast 34

Gene

TOC34

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73GTP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi49 – 54GTP1 Publication6
Nucleotide bindingi210 – 211GTP1 Publication2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.5.2. 4872.

Protein family/group databases

TCDBi3.A.9.1.1. the chloroplast envelope protein translocase (cept or tic-toc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Translocase of chloroplast 34 (EC:3.6.5.-)
Alternative name(s):
34 kDa chloroplast outer envelope protein
GTP-binding protein IAP34
GTP-binding protein OEP34
Gene namesi
Name:TOC34
Synonyms:IAP34, OEP34
Encoded oniPlastid; Chloroplast
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 268CytoplasmicSequence analysisAdd BLAST268
Transmembranei269 – 289HelicalSequence analysisAdd BLAST21
Topological domaini290 – 310Chloroplast intermembraneSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi128R → A: No dimerization and reduced GTPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001447931 – 310Translocase of chloroplast 34Add BLAST310

Proteomic databases

PRIDEiQ41009.

Interactioni

Subunit structurei

Homodimer and monomer. Part of the TOC core complex that includes a protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP-binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex. Chloroplastic protein precursor such as prSS (precursor of the RuBisCO small subunit) interacts with these complexes. The TOC complex contains a specific subset of polar lipids such as digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and phosphatidylglycerol (PG). TOC34 interacts at least with TOC75.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GRF10P483472EBI-638506,EBI-1803304From a different organism.
TOC64Q9MUK56EBI-638506,EBI-638487

Protein-protein interaction databases

DIPiDIP-332N.
IntActiQ41009. 9 interactors.
MINTiMINT-2584005.

Structurei

Secondary structure

1310
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 16Combined sources5
Helixi19 – 34Combined sources16
Beta strandi39 – 47Combined sources9
Helixi52 – 60Combined sources9
Beta strandi69 – 71Combined sources3
Beta strandi78 – 84Combined sources7
Beta strandi87 – 93Combined sources7
Beta strandi97 – 99Combined sources3
Helixi105 – 114Combined sources10
Turni115 – 117Combined sources3
Beta strandi122 – 130Combined sources9
Helixi136 – 149Combined sources14
Helixi151 – 156Combined sources6
Beta strandi157 – 162Combined sources6
Helixi169 – 171Combined sources3
Helixi174 – 192Combined sources19
Helixi200 – 202Combined sources3
Beta strandi206 – 208Combined sources3
Helixi231 – 243Combined sources13
Beta strandi245 – 247Combined sources3
Helixi254 – 258Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H65X-ray2.00A/B/C1-258[»]
3BB1X-ray2.80A/B/C/D/E/F/G/H1-266[»]
ProteinModelPortaliQ41009.
SMRiQ41009.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ41009.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 261AIG1-type GAdd BLAST225

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 71Homodimerization4
Regioni128 – 133Homodimerization6

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006703. G_AIG1.
IPR027417. P-loop_NTPase.
IPR005688. Toc34.
[Graphical view]
PfamiPF04548. AIG1. 1 hit.
[Graphical view]
PIRSFiPIRSF038134. Toc34. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00991. 3a0901s02IAP34. 1 hit.
PROSITEiPS51720. G_AIG1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q41009-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQQQTVRE WSGINTFAPA TQTKLLELLG NLKQEDVNSL TILVMGKGGV
60 70 80 90 100
GKSSTVNSII GERVVSISPF QSEGPRPVMV SRSRAGFTLN IIDTPGLIEG
110 120 130 140 150
GYINDMALNI IKSFLLDKTI DVLLYVDRLD AYRVDNLDKL VAKAITDSFG
160 170 180 190 200
KGIWNKAIVA LTHAQFSPPD GLPYDEFFSK RSEALLQVVR SGASLKKDAQ
210 220 230 240 250
ASDIPVVLIE NSGRCNKNDS DEKVLPNGIA WIPHLVQTIT EVALNKSESI
260 270 280 290 300
FVDKNLIDGP NPNQRGKLWI PLIFALQYLF LAKPIEALIR RDIATETKPA
310
WETRDVGDRK
Length:310
Mass (Da):34,157
Last modified:November 1, 1996 - v1
Checksum:i00D5E79735BF041F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28341 mRNA. Translation: CAA82196.1.
L36856 mRNA. Translation: AAC25785.1.
PIRiB55171.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28341 mRNA. Translation: CAA82196.1.
L36856 mRNA. Translation: AAC25785.1.
PIRiB55171.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H65X-ray2.00A/B/C1-258[»]
3BB1X-ray2.80A/B/C/D/E/F/G/H1-266[»]
ProteinModelPortaliQ41009.
SMRiQ41009.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-332N.
IntActiQ41009. 9 interactors.
MINTiMINT-2584005.

Protein family/group databases

TCDBi3.A.9.1.1. the chloroplast envelope protein translocase (cept or tic-toc) family.

Proteomic databases

PRIDEiQ41009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.6.5.2. 4872.

Miscellaneous databases

EvolutionaryTraceiQ41009.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006703. G_AIG1.
IPR027417. P-loop_NTPase.
IPR005688. Toc34.
[Graphical view]
PfamiPF04548. AIG1. 1 hit.
[Graphical view]
PIRSFiPIRSF038134. Toc34. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00991. 3a0901s02IAP34. 1 hit.
PROSITEiPS51720. G_AIG1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTOC34_PEA
AccessioniPrimary (citable) accession number: Q41009
Secondary accession number(s): Q41029
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.