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Protein

Translocase of chloroplast 34

Gene

TOC34

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei73 – 731GTP1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi49 – 546GTP1 Publication
Nucleotide bindingi210 – 2112GTP1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.5.2. 4872.

Protein family/group databases

TCDBi3.A.9.1.1. the chloroplast envelope protein translocase (cept or tic-toc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Translocase of chloroplast 34 (EC:3.6.5.-)
Alternative name(s):
34 kDa chloroplast outer envelope protein
GTP-binding protein IAP34
GTP-binding protein OEP34
Gene namesi
Name:TOC34
Synonyms:IAP34, OEP34
Encoded oniPlastid; Chloroplast
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 268268CytoplasmicSequence analysisAdd
BLAST
Transmembranei269 – 28921HelicalSequence analysisAdd
BLAST
Topological domaini290 – 31021Chloroplast intermembraneSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Plastid outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi128 – 1281R → A: No dimerization and reduced GTPase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 310310Translocase of chloroplast 34PRO_0000144793Add
BLAST

Interactioni

Subunit structurei

Homodimer and monomer. Part of the TOC core complex that includes a protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP-binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex. Chloroplastic protein precursor such as prSS (precursor of the RuBisCO small subunit) interacts with these complexes. The TOC complex contains a specific subset of polar lipids such as digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and phosphatidylglycerol (PG). TOC34 interacts at least with TOC75.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GRF10P483472EBI-638506,EBI-1803304From a different organism.
TOC64Q9MUK56EBI-638506,EBI-638487

Protein-protein interaction databases

DIPiDIP-332N.
IntActiQ41009. 9 interactions.
MINTiMINT-2584005.

Structurei

Secondary structure

1
310
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 165Combined sources
Helixi19 – 3416Combined sources
Beta strandi39 – 479Combined sources
Helixi52 – 609Combined sources
Beta strandi69 – 713Combined sources
Beta strandi78 – 847Combined sources
Beta strandi87 – 937Combined sources
Beta strandi97 – 993Combined sources
Helixi105 – 11410Combined sources
Turni115 – 1173Combined sources
Beta strandi122 – 1309Combined sources
Helixi136 – 14914Combined sources
Helixi151 – 1566Combined sources
Beta strandi157 – 1626Combined sources
Helixi169 – 1713Combined sources
Helixi174 – 19219Combined sources
Helixi200 – 2023Combined sources
Beta strandi206 – 2083Combined sources
Helixi231 – 24313Combined sources
Beta strandi245 – 2473Combined sources
Helixi254 – 2585Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1H65X-ray2.00A/B/C1-258[»]
3BB1X-ray2.80A/B/C/D/E/F/G/H1-266[»]
ProteinModelPortaliQ41009.
SMRiQ41009. Positions 7-258.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ41009.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 261225AIG1-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni68 – 714Homodimerization
Regioni128 – 1336Homodimerization

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006703. G_AIG1.
IPR027417. P-loop_NTPase.
IPR005688. Toc34.
[Graphical view]
PfamiPF04548. AIG1. 1 hit.
[Graphical view]
PIRSFiPIRSF038134. Toc34. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00991. 3a0901s02IAP34. 1 hit.
PROSITEiPS51720. G_AIG1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q41009-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASQQQTVRE WSGINTFAPA TQTKLLELLG NLKQEDVNSL TILVMGKGGV
60 70 80 90 100
GKSSTVNSII GERVVSISPF QSEGPRPVMV SRSRAGFTLN IIDTPGLIEG
110 120 130 140 150
GYINDMALNI IKSFLLDKTI DVLLYVDRLD AYRVDNLDKL VAKAITDSFG
160 170 180 190 200
KGIWNKAIVA LTHAQFSPPD GLPYDEFFSK RSEALLQVVR SGASLKKDAQ
210 220 230 240 250
ASDIPVVLIE NSGRCNKNDS DEKVLPNGIA WIPHLVQTIT EVALNKSESI
260 270 280 290 300
FVDKNLIDGP NPNQRGKLWI PLIFALQYLF LAKPIEALIR RDIATETKPA
310
WETRDVGDRK
Length:310
Mass (Da):34,157
Last modified:November 1, 1996 - v1
Checksum:i00D5E79735BF041F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28341 mRNA. Translation: CAA82196.1.
L36856 mRNA. Translation: AAC25785.1.
PIRiB55171.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28341 mRNA. Translation: CAA82196.1.
L36856 mRNA. Translation: AAC25785.1.
PIRiB55171.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1H65X-ray2.00A/B/C1-258[»]
3BB1X-ray2.80A/B/C/D/E/F/G/H1-266[»]
ProteinModelPortaliQ41009.
SMRiQ41009. Positions 7-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-332N.
IntActiQ41009. 9 interactions.
MINTiMINT-2584005.

Protein family/group databases

TCDBi3.A.9.1.1. the chloroplast envelope protein translocase (cept or tic-toc) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.6.5.2. 4872.

Miscellaneous databases

EvolutionaryTraceiQ41009.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR006703. G_AIG1.
IPR027417. P-loop_NTPase.
IPR005688. Toc34.
[Graphical view]
PfamiPF04548. AIG1. 1 hit.
[Graphical view]
PIRSFiPIRSF038134. Toc34. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00991. 3a0901s02IAP34. 1 hit.
PROSITEiPS51720. G_AIG1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A constituent of the chloroplast import complex represents a new type of GTP-binding protein."
    Seedorf M., Waegemann K., Soll J.
    Plant J. 7:401-411(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Miranda.
    Tissue: Leaf.
  2. "Identification of two GTP-binding proteins in the chloroplast protein import machinery."
    Kessler F., Blobel G., Patel H.V., Schnell D.J.
    Science 266:1035-1039(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 119-139 AND 157-180.
  3. "Crystal structure of pea Toc34, a novel GTPase of the chloroplast protein translocon."
    Sun Y.-J., Forouhar F., Li Hm H.-M., Tu S.-L., Yeh Y.-H., Kao S., Shr H.-L., Chou C.-C., Chen C., Hsiao C.-D.
    Nat. Struct. Biol. 9:95-100(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 1-258 IN COMPLEX WITH GTP, HOMODIMERIZATION, MUTAGENESIS OF ARG-128.

Entry informationi

Entry nameiTOC34_PEA
AccessioniPrimary (citable) accession number: Q41009
Secondary accession number(s): Q41029
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: November 1, 1996
Last modified: October 14, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.