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Protein

Monodehydroascorbate reductase

Gene
N/A
Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of monodehydroascorbate to ascorbate, oxidizing NADH in the process.

Catalytic activityi

NADH + 2 monodehydroascorbate = NAD+ + 2 ascorbate.1 Publication

Cofactori

FADUniRule annotationBy similarity

Kineticsi

  1. KM=5.3 µM for NADH
  2. KM=21.5 µM for NADPH
  3. KM=5.7 µM for monodehydroascorbate
  1. Vmax=312 µmol/min/mg enzyme for NADH oxidation reaction
  2. Vmax=40.9 µmol/min/mg enzyme for NADPH oxidation reaction

pH dependencei

Optimum pH is 7.5-9.5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39FADBy similarity1
Binding sitei46FADBy similarity1
Binding sitei51FADBy similarity1
Binding sitei94FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei194NADBy similarity1
Binding sitei200NADBy similarity1
Binding sitei200NADPBy similarity1
Binding sitei259NAD; via amide nitrogenBy similarity1
Binding sitei259NADP; via amide nitrogenBy similarity1
Binding sitei296FADBy similarity1
Binding sitei314FAD; via amide nitrogenBy similarity1
Binding sitei318AscorbateBy similarity1
Binding sitei347FAD; via carbonyl oxygenBy similarity1
Binding sitei347NAD; via carbonyl oxygenBy similarity1
Binding sitei347NADP; via carbonyl oxygenBy similarity1
Binding sitei349AscorbateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 15FADBy similarity4
Nucleotide bindingi145 – 146FADBy similarity2
Nucleotide bindingi170 – 176NADBy similarity7
Nucleotide bindingi172 – 176NADPBy similarity5
Nucleotide bindingi312 – 313NADBy similarity2
Nucleotide bindingi312 – 313NADPBy similarity2

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Monodehydroascorbate reductase (EC:1.6.5.4)
Short name:
MDAR
Alternative name(s):
Ascorbate free radical reductase
Short name:
AFR reductase
OrganismiPisum sativum (Garden pea)Imported
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002091431 – 433Monodehydroascorbate reductaseAdd BLAST433

Expressioni

Tissue specificityi

Expressed at relatively low levels in all tissues examined.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ40977
SMRiQ40977
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Family and domain databases

Gene3Di3.30.390.30, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
SSF55424 SSF55424, 1 hit

Sequencei

Sequence statusi: Complete.

Q40977-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHSFKYIII GGGVSAGYAA REFVKQGVHP GELAIISKEA VAPYERPALS
60 70 80 90 100
KAYLFPESPA RLPGFHTCVG SGGERLLPEW YSEKGIQLYL STEIVSADLA
110 120 130 140 150
AKFLKSANGE HFDYQTLVIA TGSAVIRLTD FGVIGANAKN IFYLREVDDA
160 170 180 190 200
DKLYEAIKRK KNAKRVVVGG GYIGLELSAV LKLNDLDVTM VYPEPWCMPR
210 220 230 240 250
LFTSEIAAFY EGYYANKGIN IIKGTVAVGF TANSDGEVKE VKLKDGRVLE
260 270 280 290 300
ADIVIVGVGG RPQISLFKGQ VEEQHGGIKT DSFFKTSVPD VYAVGDVATF
310 320 330 340 350
PLKLYNDVRR VEHVDHARKS AEQAAKAIFA ADVGKSVEEY DYLPYFYSRS
360 370 380 390 400
FDLSWQFYGD NVGETVLFGD NDPASSKPKF GTYWIKEGKV VGAFLEGGTP
410 420 430
DENKAIAKVA RAKPAVEDVN QLAEEGLSFA SKI
Length:433
Mass (Da):47,309
Last modified:November 1, 1996 - v1
Checksum:i5435C1F9E2BFCC97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U06461 mRNA Translation: AAA60979.1
PIRiA55333

Similar proteinsi

Entry informationi

Entry nameiMDAR_PEA
AccessioniPrimary (citable) accession number: Q40977
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

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