Q40963 (MPA5B_PHLPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Pollen allergen Phl p 5b Alternative name(s): Allergen Phl p Vb Allergen=Phl p 5b
Organism	Phleum pratense (Common timothy)
Taxonomic identifier	15957 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Poeae › Alopecurinae › Phleum

Protein attributes

Sequence length	284 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has ribonuclease activity. May be involved in host-pathogen interactions.
Subunit structure	Homodimer; disulfide-linked. Ref.3
Allergenic properties	Causes an allergic reaction in human. Causes grass pollen allergy.
Sequence similarities	Belongs to the Poa p IX/Phl p VI allergen family.

Ontologies

Keywords
Disease	Allergen
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	type I hypersensitivity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

‹1 – 19

›19

Potential

Chain

20 – 284

265

Pollen allergen Phl p 5b

PRO_0000021746

Amino acid modifications

Disulfide bond

205

Interchain Ref.3

Experimental info

Non-terminal residue

1

1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

284

Helix Strand Turn

Details...

Helix

152 – 155

4

Helix

158 – 173

16

Helix

177 – 179

3

Helix

180 – 195

16

Turn

196 – 198

3

Helix

203 – 205

3

Helix

207 – 218

12

Turn

219 – 222

4

Helix

225 – 227

3

Helix

228 – 250

23

Sequences

	Sequence		Length	Mass (Da)
	Q40963 [UniParc]. Last modified July 15, 1998. Version 2. Checksum: E949FB3E0985295E	FASTA	284	28,002
	10 20 30 40 50 60 AAAAVPRRGP RGGPGRSYTA DAGYAPATPA AAGAAAGKAT TEEQKLIEDI NVGFKAAVAA 70 80 90 100 110 120 AASVPAADKF KTFEAAFTSS SKAAAAKAPG LVPKLDAAYS VAYKAAVGAT PEAKFDSFVA 130 140 150 160 170 180 SLTEALRVIA GALEVHAVKP VTEEPGMAKI PAGELQIIDK IDAAFKVAAT AAATAPADDK 190 200 210 220 230 240 FTVFEAAFNK AIKESTGGAY DTYKCIPSLE AAVKQAYAAT VAAAPQVKYA VFEAALTKAI 250 260 270 280 TAMSEVQKVS QPATGAATVA AGAATTAAGA ASGAATVAAG GYKV

References

[1]	"Major allergen Phl p Vb in timothy grass is a novel pollen RNase." Bufe A., Schramm G., Keown M.B., Schlaak M., Becker W.M. FEBS Lett. 363:6-12(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Agrostideae. Tissue: Pollen.
[2]	Bufe A. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Structure of the functional domain of the major grass-pollen allergen Phlp 5b." Rajashankar K., Bufe A., Weber W., Eschenburg S., Lindner B., Betzel C. Acta Crystallogr. D 58:1175-1181(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.98 ANGSTROMS) OF 150-251, SUBUNIT, DISULFIDE BOND.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z27083 mRNA. Translation: CAA81609.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1L3P	X-ray	1.98	A	150-251	[»]

ProteinModelPortal

Q40963.

SMR

Q40963. Positions 39-138, 150-251.

ModBase

Search...

Protein family/group databases

Allergome

558. Phl p 5.
567. Phl p 5.0201.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

Gramene

Q40963.

Family and domain databases

Gene3D

1.20.120.320. 2 hits.

InterPro

IPR001778. POA_allergen.
IPR002914. POA_allergen_N.
[Graphical view]

Pfam

PF01620. Pollen_allerg_2. 2 hits.
[Graphical view]

PRINTS

PR00833. POAALLERGEN.

SUPFAM

SSF81736. POA_allergen_N. 2 hits.

ProtoNet

Search...

Other

EvolutionaryTrace

Q40963.

Entry information

Entry name

MPA5B_PHLPR

Accession

Primary (citable) accession number: Q40963

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 15, 1998
Last modified:	April 3, 2013
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program