Q40910 (PAL4_POPKI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine ammonia-lyase G4
EC=4.3.1.24

Gene names

Name:

PALG4

Organism

Populus kitakamiensis (Aspen) (Populus sieboldii x Populus grandidentata)

Taxonomic identifier

34292 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Malpighiales › Salicaceae › Saliceae › Populus

Protein attributes

Sequence length	571 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.
Catalytic activity	L-phenylalanine = trans-cinnamate + ammonia.
Pathway	Phenylpropanoid metabolism; trans-cinnamate biosynthesis; trans-cinnamate from L-phenylalanine: step 1/1.
Subcellular location	Cytoplasm Probable.
Post-translational modification	Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly By similarity.
Sequence similarities	Belongs to the PAL/histidase family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Cellular component	Cytoplasm
Molecular function	Lyase
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: InterPro biosynthetic process Inferred from electronic annotation. Source: InterPro phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ammonia-lyase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 571	›571	Phenylalanine ammonia-lyase G4		PRO_0000215415
Amino acid modifications
	Modified residue	57	1	2,3-didehydroalanine (Ser) By similarity
	Cross-link	56 ↔ 58		5-imidazolinone (Ala-Gly) By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q40910 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 6B76CC92D2DB425F	FASTA	571	62,484
	10 20 30 40 50 60 STHTLPHSAS RAAMLVRINT LLQGYSGIRF EILEAITKLL NHNITPCLPL RGTITASGDL 70 80 90 100 110 120 VPLSYIAGLL TGRPNSKAVG PNGETMAAAE AFTLAGINGG FFELQPKEGL ALVNGTAVGS 130 140 150 160 170 180 GLASMVLFET NVLAILSEVL SAIFAEVMQG KPEFTDHLTH KLKHHPGQIE AAAVMEHILD 190 200 210 220 230 240 GSSYVKAAQK LHEIDPLQKP KQDRYALRTS PQWLGPLIEV IRTSTKMIER EINSVNDNPL 250 260 270 280 290 300 IDVSRNKALH GGNFPGSPIG VSMDNTLVLA IASIGKLMFA QFSELVNDYY NNGLPSNLTG 310 320 330 340 350 360 GRNPSLDYGF KGAEIAMASY CSELQFLANP VTNHVQSAEQ HNQDVNSLGL ISARKTAEAV 370 380 390 400 410 420 EILNVMSTTW LVALCQAIDL RHIEENLKNT VKNTVSQVAK RVLTMGFNGE LHPSRFCEKD 430 440 450 460 470 480 LLKVVDREYV FTYIDDPCSA TYPLMQKLRQ VLVDHALMNG EKEQNSSTSI FQKIGAFEEE 490 500 510 520 530 540 LKILLPKEVE SARLELENGN PAIPNRITDR RSYPLYKFVR EELGTVLLTG EKVGSPGEEF 550 560 570 DKVFTAICAG KLIDPCWSVL KEWNGAPLPL C

References

[1]	"Characterization of the structure and determination of mRNA levels of the phenylalanine ammonia-lyase gene family from Populus kitakamiensis." Osakabe Y., Osakabe K., Kawai S., Katayama Y., Morohoshi N. Plant Mol. Biol. 28:1133-1141(1995) [PubMed: 7548831] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D43803 Genomic DNA. Translation: BAA07861.1.
3D structure databases
ProteinModelPortal	Q40910.
SMR	Q40910. Positions 1-571.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008948. L-Aspartase-like. IPR024083. L-Aspartase-like_N. IPR001106. Phe/His_NH3-lyase. IPR022313. Phe/His_NH3-lyase_AS. IPR005922. Phe_NH3-lyase. IPR023144. Phe_NH3-lyase_shielding_dom. [Graphical view]
Gene3D	G3DSA:1.10.275.10. G3DSA:1.10.275.10. 1 hit. G3DSA:1.10.274.20. Phe_NH3-lyase_shielding_dom. 1 hit.
Pfam	PF00221. PAL. 1 hit. [Graphical view]
SUPFAM	SSF48557. L-Aspartase-like. 1 hit.
TIGRFAMs	TIGR01226. Phe_am_lyase. 1 hit.
PROSITE	PS00488. PAL_HISTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PAL4_POPKI

Accession

Primary (citable) accession number: Q40910

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	July 27, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program