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Q40884 (MPK_PETHY) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase homolog 1

EC=2.7.11.24
Alternative name(s):
PMEK1
Gene names
Name:MPK1
Synonyms:MEK1
OrganismPetunia hybrida (Petunia)
Taxonomic identifier4102 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

Protein attributes

Sequence length384 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by tyrosine and threonine phosphorylation By similarity.

Tissue specificity

Expressed in vegetative organs such as leaf, root, or stem. In the reproductive organs, it is found in the ovary, but not in the stamen.

Developmental stage

Present during flower development prior to fertilization. At early stages of ovule development, the expression is uniform throughout the ovule. At a later stage, is localized adjacent to the embryo sac. When the flower opens and pollen is released, it is found in the whole ovule and in the outer layer of the placenta.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 384384Mitogen-activated protein kinase homolog 1
PRO_0000186320

Regions

Domain32 – 319288Protein kinase
Nucleotide binding38 – 469ATP By similarity
Motif191 – 1933TXY

Sites

Active site1581Proton acceptor By similarity
Binding site611ATP By similarity

Amino acid modifications

Modified residue1911Phosphothreonine By similarity
Modified residue1931Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q40884 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 7536CC960390EC58

FASTA38444,445
        10         20         30         40         50         60 
MATPVEPPNG IRTPGKHYYS MWQSLFEIDT KYVPIKPIGR GAYGIVCSSV NRETNEKVAI 

        70         80         90        100        110        120 
KKINNAFENR IDALRTLREL KLLRHLRHEN VIALKDVMMP IQRRSFKDVY LVYELMDTDL 

       130        140        150        160        170        180 
HQIIKSSQTL SNDHCQYFLF QLLRGLKYLH SANILHRDLK PGNLLINANC DLKICDFGLA 

       190        200        210        220        230        240 
RTSSGKDQFM TEYVVTRWYR APELLLCCDN YGTSIDVWSV GCIFADVLGR KPVFPGTECL 

       250        260        270        280        290        300 
NQLKLIINIL GSQREEDIEF IDNPKARKYI KSLPYSPGTP FSRLYPNAHP LAIDLLQRML 

       310        320        330        340        350        360 
VFDPSKRISV MEALQHPYMS PLYDPNTDPP AQVPINLDID EDLVEETIRE MMWEEILHYH 

       370        380 
PEAAVALLWK LFYELLLVPS RHRP 

« Hide

References

[1]"A homologue of the MAP/ERK family of protein kinase genes is expressed in vegetative and in female reproductive organs of Petunia hybrida."
Decroocq-Ferrant V., Decroocq S., van Went J., Schmidt E., Kreis M.
Plant Mol. Biol. 27:339-350(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Ovule.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83440 mRNA. Translation: CAA58466.1.
PIRS52989.

3D structure databases

ProteinModelPortalQ40884.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ40884.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.7.11.24. 4700.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPK_PETHY
AccessionPrimary (citable) accession number: Q40884
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families