Reviewed, UniProtKB/Swiss-Prot Q40884 (MAPK_PETHY)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase homolog 1
    EC=2.7.11.24
Alternative name(s):
    PMEK1

Gene names

Name:

MEK1

Organism

Petunia hybrida (Petunia)

Taxonomic identifier

4102 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Petunioideae › Petunia

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Tissue specificity	Expressed in vegetative organs such as leaf, root, or stem. In the reproductive organs, it is found in the ovary, but not in the stamen.
Developmental stage	Present during flower development prior to fertilization. At early stages of ovule development, the expression is uniform throughout the ovule. At a later stage, is localized adjacent to the embryo sac. When the flower opens and pollen is released, it is found in the whole ovule and in the outer layer of the placenta.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 384	384	Mitogen-activated protein kinase homolog 1		PRO_0000186320
Regions
	Domain	32 – 319	288	Protein kinase
	Nucleotide binding	38 – 46	9	ATP By similarity
	Motif	191 – 193	3	TXY
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	61	1	ATP By similarity
Amino acid modifications
	Modified residue	191	1	Phosphothreonine By similarity
	Modified residue	193	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q40884-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7536CC960390EC58	FASTA	384	44,445
	10 20 30 40 50 60 MATPVEPPNG IRTPGKHYYS MWQSLFEIDT KYVPIKPIGR GAYGIVCSSV NRETNEKVAI 70 80 90 100 110 120 KKINNAFENR IDALRTLREL KLLRHLRHEN VIALKDVMMP IQRRSFKDVY LVYELMDTDL 130 140 150 160 170 180 HQIIKSSQTL SNDHCQYFLF QLLRGLKYLH SANILHRDLK PGNLLINANC DLKICDFGLA 190 200 210 220 230 240 RTSSGKDQFM TEYVVTRWYR APELLLCCDN YGTSIDVWSV GCIFADVLGR KPVFPGTECL 250 260 270 280 290 300 NQLKLIINIL GSQREEDIEF IDNPKARKYI KSLPYSPGTP FSRLYPNAHP LAIDLLQRML 310 320 330 340 350 360 VFDPSKRISV MEALQHPYMS PLYDPNTDPP AQVPINLDID EDLVEETIRE MMWEEILHYH 370 380 PEAAVALLWK LFYELLLVPS RHRP

References

[1]

"A homologue of the MAP/ERK family of protein kinase genes is expressed in vegetative and in female reproductive organs of Petunia hybrida." Decroocq-Ferrant V., Decroocq S., van Went J., Schmidt E., Kreis M. Plant Mol. Biol. 27:339-350(1995) [PubMed: 7888623] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Ovule.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83440 mRNA. Translation: CAA58466.1.
PIR	S52989.
3D structure databases
HSSP	HSSP built from PDB template 1KV1 based on UniProtKB Q16539.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.24. 2263.
Family and domain databases
InterPro	IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAPK_PETHY

Accession

Primary (citable) accession number: Q40884

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)