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Protein

Mitogen-activated protein kinase homolog 1

Gene

MPK1

Organism
Petunia hybrida (Petunia)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ATPPROSITE-ProRule annotation1
Active sitei158Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 46ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 4700.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase homolog 1 (EC:2.7.11.24)
Alternative name(s):
PMEK1
Gene namesi
Name:MPK1
Synonyms:MEK1
OrganismiPetunia hybrida (Petunia)
Taxonomic identifieri4102 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863201 – 384Mitogen-activated protein kinase homolog 1Add BLAST384

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei191PhosphothreonineBy similarity1
Modified residuei193PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Expressed in vegetative organs such as leaf, root, or stem. In the reproductive organs, it is found in the ovary, but not in the stamen.

Developmental stagei

Present during flower development prior to fertilization. At early stages of ovule development, the expression is uniform throughout the ovule. At a later stage, is localized adjacent to the embryo sac. When the flower opens and pollen is released, it is found in the whole ovule and in the outer layer of the placenta.

Structurei

3D structure databases

ProteinModelPortaliQ40884.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 319Protein kinasePROSITE-ProRule annotationAdd BLAST288

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi191 – 193TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q40884-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPVEPPNG IRTPGKHYYS MWQSLFEIDT KYVPIKPIGR GAYGIVCSSV
60 70 80 90 100
NRETNEKVAI KKINNAFENR IDALRTLREL KLLRHLRHEN VIALKDVMMP
110 120 130 140 150
IQRRSFKDVY LVYELMDTDL HQIIKSSQTL SNDHCQYFLF QLLRGLKYLH
160 170 180 190 200
SANILHRDLK PGNLLINANC DLKICDFGLA RTSSGKDQFM TEYVVTRWYR
210 220 230 240 250
APELLLCCDN YGTSIDVWSV GCIFADVLGR KPVFPGTECL NQLKLIINIL
260 270 280 290 300
GSQREEDIEF IDNPKARKYI KSLPYSPGTP FSRLYPNAHP LAIDLLQRML
310 320 330 340 350
VFDPSKRISV MEALQHPYMS PLYDPNTDPP AQVPINLDID EDLVEETIRE
360 370 380
MMWEEILHYH PEAAVALLWK LFYELLLVPS RHRP
Length:384
Mass (Da):44,445
Last modified:November 1, 1996 - v1
Checksum:i7536CC960390EC58
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83440 mRNA. Translation: CAA58466.1.
PIRiS52989.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83440 mRNA. Translation: CAA58466.1.
PIRiS52989.

3D structure databases

ProteinModelPortaliQ40884.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.7.11.24. 4700.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPK_PETHY
AccessioniPrimary (citable) accession number: Q40884
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.