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Q40884

- MPK_PETHY

UniProt

Q40884 - MPK_PETHY

Protein

Mitogen-activated protein kinase homolog 1

Gene

MPK1

Organism
Petunia hybrida (Petunia)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by tyrosine and threonine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei61 – 611ATPPROSITE-ProRule annotation
    Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi38 – 469ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24. 4700.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase homolog 1 (EC:2.7.11.24)
    Alternative name(s):
    PMEK1
    Gene namesi
    Name:MPK1
    Synonyms:MEK1
    OrganismiPetunia hybrida (Petunia)
    Taxonomic identifieri4102 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 384384Mitogen-activated protein kinase homolog 1PRO_0000186320Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei191 – 1911PhosphothreonineBy similarity
    Modified residuei193 – 1931PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiQ40884.

    Expressioni

    Tissue specificityi

    Expressed in vegetative organs such as leaf, root, or stem. In the reproductive organs, it is found in the ovary, but not in the stamen.

    Developmental stagei

    Present during flower development prior to fertilization. At early stages of ovule development, the expression is uniform throughout the ovule. At a later stage, is localized adjacent to the embryo sac. When the flower opens and pollen is released, it is found in the whole ovule and in the outer layer of the placenta.

    Structurei

    3D structure databases

    ProteinModelPortaliQ40884.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 319288Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi191 – 1933TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q40884-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATPVEPPNG IRTPGKHYYS MWQSLFEIDT KYVPIKPIGR GAYGIVCSSV    50
    NRETNEKVAI KKINNAFENR IDALRTLREL KLLRHLRHEN VIALKDVMMP 100
    IQRRSFKDVY LVYELMDTDL HQIIKSSQTL SNDHCQYFLF QLLRGLKYLH 150
    SANILHRDLK PGNLLINANC DLKICDFGLA RTSSGKDQFM TEYVVTRWYR 200
    APELLLCCDN YGTSIDVWSV GCIFADVLGR KPVFPGTECL NQLKLIINIL 250
    GSQREEDIEF IDNPKARKYI KSLPYSPGTP FSRLYPNAHP LAIDLLQRML 300
    VFDPSKRISV MEALQHPYMS PLYDPNTDPP AQVPINLDID EDLVEETIRE 350
    MMWEEILHYH PEAAVALLWK LFYELLLVPS RHRP 384
    Length:384
    Mass (Da):44,445
    Last modified:November 1, 1996 - v1
    Checksum:i7536CC960390EC58
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83440 mRNA. Translation: CAA58466.1.
    PIRiS52989.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83440 mRNA. Translation: CAA58466.1 .
    PIRi S52989.

    3D structure databases

    ProteinModelPortali Q40884.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q40884.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 2.7.11.24. 4700.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A homologue of the MAP/ERK family of protein kinase genes is expressed in vegetative and in female reproductive organs of Petunia hybrida."
      Decroocq-Ferrant V., Decroocq S., van Went J., Schmidt E., Kreis M.
      Plant Mol. Biol. 27:339-350(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ovule.

    Entry informationi

    Entry nameiMPK_PETHY
    AccessioniPrimary (citable) accession number: Q40884
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3