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Q40884

- MPK_PETHY

UniProt

Q40884 - MPK_PETHY

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Protein
Mitogen-activated protein kinase homolog 1
Gene
MPK1, MEK1
Organism
Petunia hybrida (Petunia)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611ATP By similarity
Active sitei158 – 1581Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi38 – 469ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 4700.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase homolog 1 (EC:2.7.11.24)
Alternative name(s):
PMEK1
Gene namesi
Name:MPK1
Synonyms:MEK1
OrganismiPetunia hybrida (Petunia)
Taxonomic identifieri4102 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaePetunioideaePetunia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 384384Mitogen-activated protein kinase homolog 1
PRO_0000186320Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei191 – 1911Phosphothreonine By similarity
Modified residuei193 – 1931Phosphotyrosine By similarity

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ40884.

Expressioni

Tissue specificityi

Expressed in vegetative organs such as leaf, root, or stem. In the reproductive organs, it is found in the ovary, but not in the stamen.

Developmental stagei

Present during flower development prior to fertilization. At early stages of ovule development, the expression is uniform throughout the ovule. At a later stage, is localized adjacent to the embryo sac. When the flower opens and pollen is released, it is found in the whole ovule and in the outer layer of the placenta.

Structurei

3D structure databases

ProteinModelPortaliQ40884.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini32 – 319288Protein kinase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi191 – 1933TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q40884-1 [UniParc]FASTAAdd to Basket

« Hide

MATPVEPPNG IRTPGKHYYS MWQSLFEIDT KYVPIKPIGR GAYGIVCSSV    50
NRETNEKVAI KKINNAFENR IDALRTLREL KLLRHLRHEN VIALKDVMMP 100
IQRRSFKDVY LVYELMDTDL HQIIKSSQTL SNDHCQYFLF QLLRGLKYLH 150
SANILHRDLK PGNLLINANC DLKICDFGLA RTSSGKDQFM TEYVVTRWYR 200
APELLLCCDN YGTSIDVWSV GCIFADVLGR KPVFPGTECL NQLKLIINIL 250
GSQREEDIEF IDNPKARKYI KSLPYSPGTP FSRLYPNAHP LAIDLLQRML 300
VFDPSKRISV MEALQHPYMS PLYDPNTDPP AQVPINLDID EDLVEETIRE 350
MMWEEILHYH PEAAVALLWK LFYELLLVPS RHRP 384
Length:384
Mass (Da):44,445
Last modified:November 1, 1996 - v1
Checksum:i7536CC960390EC58
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83440 mRNA. Translation: CAA58466.1.
PIRiS52989.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83440 mRNA. Translation: CAA58466.1 .
PIRi S52989.

3D structure databases

ProteinModelPortali Q40884.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q40884.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 2.7.11.24. 4700.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A homologue of the MAP/ERK family of protein kinase genes is expressed in vegetative and in female reproductive organs of Petunia hybrida."
    Decroocq-Ferrant V., Decroocq S., van Went J., Schmidt E., Kreis M.
    Plant Mol. Biol. 27:339-350(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovule.

Entry informationi

Entry nameiMPK_PETHY
AccessioniPrimary (citable) accession number: Q40884
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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