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Protein

Allene oxide synthase

Gene

CYP74A2

Organism
Parthenium argentatum (Guayule rubber plant)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of jasmonic acid, a growth regulator that is implicated also as a signaling molecule in plant defense. Acts on a number of unsaturated fatty-acid hydroperoxides, forming the corresponding allene oxides (By similarity).By similarity

Catalytic activityi

(9Z,11E,15Z)-(13S)-hydroperoxyoctadeca-9,11,15-trienoate = (9Z,15Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate + H2O.

Cofactori

hemeBy similarity

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921SubstrateBy similarity
Binding sitei199 – 1991Substrate
Binding sitei282 – 2821Substrate
Metal bindingi426 – 4261Iron (heme axial ligand)

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Allene oxide synthase (EC:4.2.1.92)
Alternative name(s):
Cytochrome P450 74A2
Rubber particle protein
Short name:
RPP
Gene namesi
Name:CYP74A2
Synonyms:RPP30
OrganismiParthenium argentatum (Guayule rubber plant)
Taxonomic identifieri35935 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeParthenium

Subcellular locationi


    Note: Rubber particle.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 473473Allene oxide synthasePRO_0000052128Add
BLAST

Structurei

Secondary structure

1
473
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 53Combined sources
Helixi20 – 3112Combined sources
Helixi35 – 4713Combined sources
Beta strandi50 – 556Combined sources
Turni60 – 623Combined sources
Beta strandi67 – 715Combined sources
Turni74 – 763Combined sources
Helixi77 – 815Combined sources
Turni83 – 853Combined sources
Beta strandi94 – 963Combined sources
Helixi100 – 1034Combined sources
Helixi109 – 1113Combined sources
Helixi117 – 13317Combined sources
Turni134 – 1363Combined sources
Helixi137 – 15923Combined sources
Helixi165 – 18117Combined sources
Helixi185 – 1873Combined sources
Turni189 – 1924Combined sources
Helixi194 – 20613Combined sources
Helixi207 – 2093Combined sources
Helixi216 – 2238Combined sources
Beta strandi224 – 2263Combined sources
Helixi230 – 2323Combined sources
Helixi234 – 24714Combined sources
Helixi249 – 2579Combined sources
Helixi262 – 27413Combined sources
Helixi276 – 29520Combined sources
Helixi297 – 31418Combined sources
Beta strandi316 – 3183Combined sources
Helixi321 – 3255Combined sources
Helixi328 – 34013Combined sources
Beta strandi346 – 3538Combined sources
Beta strandi355 – 3584Combined sources
Beta strandi363 – 3664Combined sources
Beta strandi371 – 3755Combined sources
Helixi376 – 3794Combined sources
Turni383 – 3853Combined sources
Beta strandi386 – 3883Combined sources
Turni394 – 3974Combined sources
Helixi399 – 4079Combined sources
Beta strandi421 – 4233Combined sources
Helixi429 – 44618Combined sources
Beta strandi447 – 4559Combined sources
Beta strandi458 – 4603Combined sources
Beta strandi462 – 47110Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DAMX-ray2.40A1-473[»]
3DANX-ray1.80A1-473[»]
3DBMX-ray2.60A1-473[»]
ProteinModelPortaliQ40778.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ40778.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni275 – 2762Substrate binding
Regioni343 – 3464Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Family and domain databases

Gene3Di1.10.630.10. 2 hits.
InterProiIPR001128. Cyt_P450.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

Q40778-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPSSKPLRE IPGSYGIPFF QPIKDRLEYF YGTGGRDEYF RSRMQKYQST
60 70 80 90 100
VFRANMPPGP FVSSNPKVIV LLDAKSFPIL FDVSKVEKKD LFTGTYMPST
110 120 130 140 150
KLTGGYRVLS YLDPSEPRHA QLKNLLFFML KNSSNRVIPQ FETTYTELFE
160 170 180 190 200
GLEAELAKNG KAAFNDVGEQ AAFRFLGRAY FNSNPEETKL GTSAPTLISS
210 220 230 240 250
WVLFNLAPTL DLGLPWFLQE PLLHTFRLPA FLIKSTYNKL YDYFQSVATP
260 270 280 290 300
VMEQAEKLGV PKDEAVHNIL FAVCFNTFGG VKILFPNTLK WIGLAGENLH
310 320 330 340 350
TQLAEEIRGA IKSYGDGNVT LEAIEQMPLT KSVVYESLRI EPPVPPQYGK
360 370 380 390 400
AKSNFTIESH DATFEVKKGE MLFGYQPFAT KDPKVFDRPE EYVPDRFVGD
410 420 430 440 450
GEALLKYVWW SNGPETESPT VENKQCAGKD FVVLITRLFV IELFRRYDSF
460 470
EIELGESPLG AAVTLTFLKR ASI
Length:473
Mass (Da):53,476
Last modified:December 16, 2008 - v2
Checksum:i658D396763ECAA90
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti392 – 3921Y → F AA sequence (PubMed:7721745).Curated
Sequence conflicti415 – 4151E → EE AA sequence (PubMed:9660772).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78166 mRNA. Translation: CAA55025.2.
PIRiA56377.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78166 mRNA. Translation: CAA55025.2.
PIRiA56377.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DAMX-ray2.40A1-473[»]
3DANX-ray1.80A1-473[»]
3DBMX-ray2.60A1-473[»]
ProteinModelPortaliQ40778.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00382.

Miscellaneous databases

EvolutionaryTraceiQ40778.

Family and domain databases

Gene3Di1.10.630.10. 2 hits.
InterProiIPR001128. Cyt_P450.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiC74A2_PARAR
AccessioniPrimary (citable) accession number: Q40778
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: December 16, 2008
Last modified: May 27, 2015
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.