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Protein

Allene oxide synthase

Gene

CYP74A2

Organism
Parthenium argentatum (Guayule rubber plant)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of jasmonic acid, a growth regulator that is implicated also as a signaling molecule in plant defense. Acts on a number of unsaturated fatty-acid hydroperoxides, forming the corresponding allene oxides (By similarity).By similarity

Catalytic activityi

(9Z,11E,15Z)-(13S)-hydroperoxyoctadeca-9,11,15-trienoate = (9Z,15Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate + H2O.

Cofactori

hemeBy similarity

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateBy similarity1
Binding sitei199Substrate1
Binding sitei282Substrate1
Metal bindingi426Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Allene oxide synthase (EC:4.2.1.92)
Alternative name(s):
Cytochrome P450 74A2
Rubber particle protein
Short name:
RPP
Gene namesi
Name:CYP74A2
Synonyms:RPP30
OrganismiParthenium argentatum (Guayule rubber plant)
Taxonomic identifieri35935 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeParthenium

Subcellular locationi


    Note: Rubber particle.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000521281 – 473Allene oxide synthaseAdd BLAST473

Structurei

Secondary structure

1473
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 5Combined sources3
Helixi20 – 31Combined sources12
Helixi35 – 47Combined sources13
Beta strandi50 – 55Combined sources6
Turni60 – 62Combined sources3
Beta strandi67 – 71Combined sources5
Turni74 – 76Combined sources3
Helixi77 – 81Combined sources5
Turni83 – 85Combined sources3
Beta strandi94 – 96Combined sources3
Helixi100 – 103Combined sources4
Helixi109 – 111Combined sources3
Helixi117 – 133Combined sources17
Turni134 – 136Combined sources3
Helixi137 – 159Combined sources23
Helixi165 – 181Combined sources17
Helixi185 – 187Combined sources3
Turni189 – 192Combined sources4
Helixi194 – 206Combined sources13
Helixi207 – 209Combined sources3
Helixi216 – 223Combined sources8
Beta strandi224 – 226Combined sources3
Helixi230 – 232Combined sources3
Helixi234 – 247Combined sources14
Helixi249 – 257Combined sources9
Helixi262 – 274Combined sources13
Helixi276 – 295Combined sources20
Helixi297 – 314Combined sources18
Beta strandi316 – 318Combined sources3
Helixi321 – 325Combined sources5
Helixi328 – 340Combined sources13
Beta strandi346 – 353Combined sources8
Beta strandi355 – 358Combined sources4
Beta strandi363 – 366Combined sources4
Beta strandi371 – 375Combined sources5
Helixi376 – 379Combined sources4
Turni383 – 385Combined sources3
Beta strandi386 – 388Combined sources3
Turni394 – 397Combined sources4
Helixi399 – 407Combined sources9
Beta strandi421 – 423Combined sources3
Helixi429 – 446Combined sources18
Beta strandi447 – 455Combined sources9
Beta strandi458 – 460Combined sources3
Beta strandi462 – 471Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DAMX-ray2.40A1-473[»]
3DANX-ray1.80A1-473[»]
3DBMX-ray2.60A1-473[»]
ProteinModelPortaliQ40778.
SMRiQ40778.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ40778.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni275 – 276Substrate binding2
Regioni343 – 346Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Family and domain databases

Gene3Di1.10.630.10. 2 hits.
InterProiIPR001128. Cyt_P450.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequencei

Sequence statusi: Complete.

Q40778-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPSSKPLRE IPGSYGIPFF QPIKDRLEYF YGTGGRDEYF RSRMQKYQST
60 70 80 90 100
VFRANMPPGP FVSSNPKVIV LLDAKSFPIL FDVSKVEKKD LFTGTYMPST
110 120 130 140 150
KLTGGYRVLS YLDPSEPRHA QLKNLLFFML KNSSNRVIPQ FETTYTELFE
160 170 180 190 200
GLEAELAKNG KAAFNDVGEQ AAFRFLGRAY FNSNPEETKL GTSAPTLISS
210 220 230 240 250
WVLFNLAPTL DLGLPWFLQE PLLHTFRLPA FLIKSTYNKL YDYFQSVATP
260 270 280 290 300
VMEQAEKLGV PKDEAVHNIL FAVCFNTFGG VKILFPNTLK WIGLAGENLH
310 320 330 340 350
TQLAEEIRGA IKSYGDGNVT LEAIEQMPLT KSVVYESLRI EPPVPPQYGK
360 370 380 390 400
AKSNFTIESH DATFEVKKGE MLFGYQPFAT KDPKVFDRPE EYVPDRFVGD
410 420 430 440 450
GEALLKYVWW SNGPETESPT VENKQCAGKD FVVLITRLFV IELFRRYDSF
460 470
EIELGESPLG AAVTLTFLKR ASI
Length:473
Mass (Da):53,476
Last modified:December 16, 2008 - v2
Checksum:i658D396763ECAA90
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti392Y → F AA sequence (PubMed:7721745).Curated1
Sequence conflicti415E → EE AA sequence (PubMed:9660772).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78166 mRNA. Translation: CAA55025.2.
PIRiA56377.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78166 mRNA. Translation: CAA55025.2.
PIRiA56377.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DAMX-ray2.40A1-473[»]
3DANX-ray1.80A1-473[»]
3DBMX-ray2.60A1-473[»]
ProteinModelPortaliQ40778.
SMRiQ40778.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00382.

Miscellaneous databases

EvolutionaryTraceiQ40778.

Family and domain databases

Gene3Di1.10.630.10. 2 hits.
InterProiIPR001128. Cyt_P450.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
SUPFAMiSSF48264. SSF48264. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiC74A2_PARAR
AccessioniPrimary (citable) accession number: Q40778
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: December 16, 2008
Last modified: November 2, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.