Reviewed, UniProtKB/Swiss-Prot Q40772 (RIP2_PHYAM)

Names and origin

Protein names

Recommended name:
    Antiviral protein 2
    EC=3.2.2.22
Alternative name(s):
    PAP-II
    Ribosome-inactivating protein
    rRNA N-glycosidase

Gene names

Name:	PAP2
Synonyms:	PAPII

Organism

Phytolacca americana (American pokeweed) (Phytolacca decandra)

Taxonomic identifier

3527 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Phytolaccaceae › Phytolacca

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits viral infection of plants. Inhibits protein synthesis in both prokaryotes and eukaryotes.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Tissue specificity	Expressed in late summer leaves.
Developmental stage	Expressed progressively with the aging of the plant.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Antiviral defense Plant defense
Domain	Signal
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 25

25

Ref.2

Chain

26 – ?

Antiviral protein 2

PRO_0000030783

Propeptide

? – 310

PRO_0000030784

Sites

Active site

197

1

By similarity

Amino acid modifications

Disulfide bond

57 ↔ 284

By similarity

Disulfide bond

106 ↔ 123

By similarity

Secondary structure

1

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310

Helix Strand Turn

Details...

Beta strand

27 – 31

5

Helix

36 – 50

15

Beta strand

51 – 57

7

Beta strand

60 – 63

4

Beta strand

66 – 69

4

Beta strand

71 – 79

9

Beta strand

82 – 89

8

Turn

90 – 92

3

Beta strand

95 – 102

8

Beta strand

105 – 110

6

Helix

118 – 122

5

Turn

123 – 125

3

Turn

132 – 134

3

Helix

142 – 149

8

Helix

152 – 154

3

Helix

159 – 169

11

Turn

177 – 179

3

Helix

180 – 200

21

Helix

202 – 210

9

Turn

211 – 213

3

Helix

222 – 228

7

Helix

231 – 240

10

Beta strand

248 – 250

3

Beta strand

260 – 262

3

Beta strand

265 – 267

3

Helix

268 – 273

6

Beta strand

276 – 279

4

Sequences

	Sequence		Length	Mass (Da)
	Q40772-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4D3BB001D7259D9F	FASTA	310	34,694
	10 20 30 40 50 60 MKMKVLEVVG LAISIWLMLT PPASSNIVFD VENATPETYS NFLTSLREAV KDKKLTCHGM 70 80 90 100 110 120 IMATTLTEQP KYVLVDLKFG SGTFTLAIRR GNLYLEGYSD IYNGKCRYRI FKDSESDAQE 130 140 150 160 170 180 TVCPGDKSKP GTQNNIPYEK SYKGMESKGG ARTKLGLGKI TLKSRMGKIY GKDATDQKQY 190 200 210 220 230 240 QKNEAEFLLI AVQMVTEASR FKYIENKVKA KFDDANGYQP DPKAISLEKN WDSVSKVIAK 250 260 270 280 290 300 VGTSGDSTVT LPGDLKDENN KPWTTATMND LKNDIMALLT HVTCKVKSSM FPEIMSYYYR 310 TSISNLGEFE

References

[1]	"Isolation and characterization of a cDNA clone encoding the pokeweed antiviral protein II from Phytolacca americana and its expression in E. coli." Poyet J.-L., Radom J., Hoeveler A. FEBS Lett. 347:268-272(1994) [PubMed: 8034016] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
[2]	"Characterization of translational inhibitors from Phytolacca americana. Amino-terminal sequence determination and antibody-inhibitor conjugates." Bjorn M.J., Larrick J., Piatak M., Wilson K.J. Biochim. Biophys. Acta 790:154-163(1984) [PubMed: 6091760] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-55. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X78628 mRNA. Translation: CAA55342.1.

PIR

S32610.
S46239.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LLN	X-ray	1.60	A	26-287	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

3.2.2.22. 257893.

Family and domain databases

InterPro

IPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]

Gene3D

G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.

Pfam

PF00161. RIP. 1 hit.
[Graphical view]

PRINTS

PR00396. SHIGARICIN.

PROSITE

PS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RIP2_PHYAM

Accession

Primary (citable) accession number: Q40772

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)