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Protein

Antiviral protein 2

Gene

PAP2

Organism
Phytolacca americana (American pokeweed) (Phytolacca decandra)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits viral infection of plants. Inhibits protein synthesis in both prokaryotes and eukaryotes.

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei197By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein synthesis inhibitor, Toxin

Keywords - Biological processi

Antiviral defense, Plant defense

Enzyme and pathway databases

BRENDAi3.2.2.22. 4806.

Names & Taxonomyi

Protein namesi
Recommended name:
Antiviral protein 2 (EC:3.2.2.22)
Alternative name(s):
PAP-II
Ribosome-inactivating protein
rRNA N-glycosidase
Gene namesi
Name:PAP2
Synonyms:PAPII
OrganismiPhytolacca americana (American pokeweed) (Phytolacca decandra)
Taxonomic identifieri3527 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesPhytolaccaceaePhytolacca

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_0000030784? – 310
Signal peptidei1 – 251 PublicationAdd BLAST25
ChainiPRO_000003078326 – ?Antiviral protein 2

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi57 ↔ 284By similarity
Disulfide bondi106 ↔ 123By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed in late summer leaves.

Developmental stagei

Expressed progressively with the aging of the plant.

Structurei

Secondary structure

1310
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi27 – 31Combined sources5
Helixi36 – 50Combined sources15
Beta strandi51 – 57Combined sources7
Beta strandi60 – 63Combined sources4
Beta strandi66 – 69Combined sources4
Beta strandi71 – 79Combined sources9
Beta strandi82 – 89Combined sources8
Turni90 – 92Combined sources3
Beta strandi95 – 102Combined sources8
Beta strandi105 – 110Combined sources6
Helixi118 – 122Combined sources5
Turni123 – 125Combined sources3
Turni132 – 134Combined sources3
Helixi142 – 149Combined sources8
Helixi152 – 154Combined sources3
Helixi159 – 169Combined sources11
Turni177 – 179Combined sources3
Helixi180 – 200Combined sources21
Helixi202 – 210Combined sources9
Turni211 – 213Combined sources3
Helixi222 – 228Combined sources7
Helixi231 – 240Combined sources10
Beta strandi248 – 250Combined sources3
Beta strandi260 – 262Combined sources3
Beta strandi265 – 267Combined sources3
Helixi268 – 273Combined sources6
Beta strandi276 – 279Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LLNX-ray1.60A26-287[»]
ProteinModelPortaliQ40772.
SMRiQ40772.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ40772.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q40772-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMKVLEVVG LAISIWLMLT PPASSNIVFD VENATPETYS NFLTSLREAV
60 70 80 90 100
KDKKLTCHGM IMATTLTEQP KYVLVDLKFG SGTFTLAIRR GNLYLEGYSD
110 120 130 140 150
IYNGKCRYRI FKDSESDAQE TVCPGDKSKP GTQNNIPYEK SYKGMESKGG
160 170 180 190 200
ARTKLGLGKI TLKSRMGKIY GKDATDQKQY QKNEAEFLLI AVQMVTEASR
210 220 230 240 250
FKYIENKVKA KFDDANGYQP DPKAISLEKN WDSVSKVIAK VGTSGDSTVT
260 270 280 290 300
LPGDLKDENN KPWTTATMND LKNDIMALLT HVTCKVKSSM FPEIMSYYYR
310
TSISNLGEFE
Length:310
Mass (Da):34,694
Last modified:November 1, 1996 - v1
Checksum:i4D3BB001D7259D9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78628 mRNA. Translation: CAA55342.1.
PIRiS32610.
S46239.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X78628 mRNA. Translation: CAA55342.1.
PIRiS32610.
S46239.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LLNX-ray1.60A26-287[»]
ProteinModelPortaliQ40772.
SMRiQ40772.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.2.2.22. 4806.

Miscellaneous databases

EvolutionaryTraceiQ40772.

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIP2_PHYAM
AccessioniPrimary (citable) accession number: Q40772
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: November 1, 1996
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.