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Protein

MADS-box transcription factor 3

Gene

MADS3

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Probable transcription factor involved in the development of floral organs. Acts as C-class protein in association with MADS58. Involved in the control of lodicule number (whorl 2), stamen specification (whorl 3) and floral meristem determinacy (whorl 4), but not in the regulation of carpel morphogenesis. Plays a more predominant role in controlling lodicule development and in specifying stamen identity than MADS58.3 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Differentiation, Flowering, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
MADS-box transcription factor 3
Alternative name(s):
OsMADS3
Protein AGAMOUS-like
RMADS222
Gene namesi
Name:MADS3
Synonyms:RAG
Ordered Locus Names:Os01g0201700, LOC_Os01g10504
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 1, cultivar: Nipponbare

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Homeotic transformation of stamens into lodicules, and ectopic development of lodicules where they do not form in the wild-type.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 236236MADS-box transcription factor 3PRO_0000229893Add
BLAST

Proteomic databases

PaxDbiQ40704.

Expressioni

Tissue specificityi

Expressed in lemmas, paleas and lodicules.1 Publication

Developmental stagei

Expressed at early stage of flower development in stamen anlagen before stamen initiation, and at later stage in lodicule and ovule primordia initiation region.2 Publications

Interactioni

Protein-protein interaction databases

IntActiQ40704. 3 interactions.
STRINGi39947.LOC_Os01g10504.1.

Structurei

3D structure databases

ProteinModelPortaliQ40704.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 6161MADS-boxPROSITE-ProRule annotationAdd
BLAST
Domaini87 – 17892K-boxPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 K-box domain.PROSITE-ProRule annotation
Contains 1 MADS-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
HOGENOMiHOG000155301.
InParanoidiQ40704.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q40704-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRGKIEIKR IENTTNRQVT FCKRRNGLLK KAYELSVLCD AEVALIVFSS
60 70 80 90 100
RGRLYEYANN SVKSTVERYK KANSDTSNSG TVAEVNAQHY QQESSKLRQQ
110 120 130 140 150
ISSLQNANSR TIVGDSINTM SLRDLKQVEN RLEKGIAKIR ARKNELLYAE
160 170 180 190 200
VEYMQKREVE LQNDNMYLRS KVVENERGQQ PLNMMGAAST SEYDHMVNNP
210 220 230
YDSRNFLQVN IMQQPQHYAH QLQPTTLQLG QQPAFN
Length:236
Mass (Da):27,141
Last modified:November 1, 1996 - v1
Checksum:i241BD9BCE2DAD35F
GO
Isoform 2 (identifier: Q40704-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     231-236: QQPAFN → SRPSISFGVDTVRTHVR

Note: No experimental confirmation available.
Show »
Length:247
Mass (Da):28,351
Checksum:i893BFAE64504C568
GO
Isoform 3 (identifier: Q40704-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     110-114: RTIVG → LTRLA
     115-236: Missing.

Note: No experimental confirmation available.
Show »
Length:114
Mass (Da):12,875
Checksum:i8B935087C09E5B69
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei110 – 1145RTIVG → LTRLA in isoform 3. 1 PublicationVSP_017777
Alternative sequencei115 – 236122Missing in isoform 3. 1 PublicationVSP_017778Add
BLAST
Alternative sequencei231 – 2366QQPAFN → SRPSISFGVDTVRTHVR in isoform 2. 1 PublicationVSP_017779

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37528 mRNA. Translation: AAA99964.1.
AY553724 mRNA. Translation: AAS60204.1.
AP003105 Genomic DNA. No translation available.
AP014957 Genomic DNA. No translation available.
AK108568 mRNA. No translation available.
PIRiS59480.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37528 mRNA. Translation: AAA99964.1.
AY553724 mRNA. Translation: AAS60204.1.
AP003105 Genomic DNA. No translation available.
AP014957 Genomic DNA. No translation available.
AK108568 mRNA. No translation available.
PIRiS59480.

3D structure databases

ProteinModelPortaliQ40704.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ40704. 3 interactions.
STRINGi39947.LOC_Os01g10504.1.

Proteomic databases

PaxDbiQ40704.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0014. Eukaryota.
COG5068. LUCA.
HOGENOMiHOG000155301.
InParanoidiQ40704.

Family and domain databases

InterProiIPR002487. TF_Kbox.
IPR002100. TF_MADSbox.
[Graphical view]
PfamiPF01486. K-box. 1 hit.
PF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS51297. K_BOX. 1 hit.
PS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Phenotypic alterations of petal and sepal by ectopic expression of a rice MADS box gene in tobacco."
    Kang H.-G., Noh Y.-S., Chung Y.-Y., Costa M.A., An K., An G.
    Plant Mol. Biol. 29:1-10(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Flower.
  2. "Molecular and phylogenetic analyses of the complete MADS-box transcription factor family in rice."
    Yao Q., Peng R., Xiong A.
    Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. "The genome sequence and structure of rice chromosome 1."
    Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.
    , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.
    Nature 420:312-316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  5. Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  6. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: cv. Nipponbare.
  7. "Identification of class B and class C floral organ identity genes from rice plants."
    Kang H.-G., Jeon J.-S., Lee S., An G.
    Plant Mol. Biol. 38:1021-1029(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Spatially and temporally regulated expression of rice MADS box genes with similarity to Arabidopsis class A, B and C genes."
    Kyozuka J., Kobayashi T., Morita M., Shimamoto K.
    Plant Cell Physiol. 41:710-718(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  9. "Ectopic expression of OsMADS3, a rice ortholog of AGAMOUS, caused a homeotic transformation of lodicules to stamens in transgenic rice plants."
    Kyozuka J., Shimamoto K.
    Plant Cell Physiol. 43:130-135(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Functional diversification of the two C-class MADS box genes OSMADS3 and OSMADS58 in Oryza sativa."
    Yamaguchi T., Lee D.-Y., Miyao A., Hirochika H., An G., Hirano H.
    Plant Cell 18:15-28(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiMADS3_ORYSJ
AccessioniPrimary (citable) accession number: Q40704
Secondary accession number(s): Q6Q7W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The transformation of lodicules into stamens is observed in overexpressing plants.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.