Q40703 (MADS4_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MADS-box transcription factor 4

Alternative name(s):
OsMADS4

Gene names

Name:	MADS4
Ordered Locus Names:	Os05g0423400, LOC_Os05g34940
ORF Names:	OJ1212_B02.5

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable transcription factor involved in the development of floral organs. B-class protein required for normal development of lodicules and stamens (whorls 2 and 3). May function as a heterodimer with MADS16. Ref.5
Subunit structure	May interact with the K-box of MADS16.
Subcellular location	Nucleus Probable.
Tissue specificity	Highly expressed in lodicules, at intermediate levels in stamens, and weakly in carpels. Expressed in pollen. Ref.1 Ref.6 Ref.7
Miscellaneous	Antisense inhibition of MADS4 expression transforms lodicules and stamens into palea/lemma-like and carpel-like organs, respectively.
Sequence similarities	Contains 1 K-box domain. Contains 1 MADS-box domain.

Ontologies

Keywords
Biological process	Differentiation Flowering Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW flower development Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: EnsemblPlants/Gramene nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 2 (identifier: Q40703-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: May be due to intron retention. No experimental confirmation available.

Isoform 1 (identifier: Q40703-2)

The sequence of this isoform differs from the canonical sequence as follows:
     64-68: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	MADS-box transcription factor 4		PRO_0000199500
Regions
	Domain	1 – 61	61	MADS-box
	Domain	89 – 175	87	K-box
Natural variations
	Alternative sequence	64 – 68	5	Missing in isoform 1.		VSP_015394
Experimental info
	Sequence conflict	26	1	A → S in AAC05723. Ref.1
	Sequence conflict	41	1	A → R in AAC05723. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 2 [UniParc]. Last modified August 30, 2005. Version 3. Checksum: FD24ED8F4ED1F213	FASTA	215	24,908
	10 20 30 40 50 60 MGRGKIEIKR IENSTNRQVT FSKRRAGILK KAREIGVLCD AEVGVVIFSS AGKLSDYCTP 70 80 90 100 110 120 KTTSVFPPLS RILEKYQTNS GKILWDEKHK SLSAEIDRVK KENDNMQIEL RHMKGEDLNS 130 140 150 160 170 180 LQPKELIAIE EALNNGQANL RDKMMDHWRM HKRNEKMLED EHKMLAFRVH QQEVELSGGI 190 200 210 RELELGYHHD DRDFAASMPF TFRVQPSHPN LQQEK
	Isoform 1 [UniParc]. Checksum: C3F940A6CC9ECCF4	FASTA	210	24,381
	10 20 30 40 50 60 MGRGKIEIKR IENSTNRQVT FSKRRAGILK KAREIGVLCD AEVGVVIFSS AGKLSDYCTP 70 80 90 100 110 120 KTTLSRILEK YQTNSGKILW DEKHKSLSAE IDRVKKENDN MQIELRHMKG EDLNSLQPKE 130 140 150 160 170 180 LIAIEEALNN GQANLRDKMM DHWRMHKRNE KMLEDEHKML AFRVHQQEVE LSGGIRELEL 190 200 210 GYHHDDRDFA ASMPFTFRVQ PSHPNLQQEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of two rice MADS box genes homologous to GLOBOSA." Chung Y.-Y., Kim S.-R., Kang H.-G., Noh Y.-S., Park M.C., Finkel D., An G. Plant Sci. 109:45-56(1995) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Immature flower.
[2]	An G. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Nipponbare.
[5]	"Identification of class B and class C floral organ identity genes from rice plants." Kang H.-G., Jeon J.-S., Lee S., An G. Plant Mol. Biol. 38:1021-1029(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Identification of a rice APETALA3 homologue by yeast two-hybrid screening." Moon Y.-H., Jung J.-Y., Kang H.-G., An G. Plant Mol. Biol. 40:167-177(1999) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INTERACTION WITH MADS16.
[7]	"Alteration of floral organ identity in rice through ectopic expression of OsMADS16." Lee S., Jeon J.-S., An K., Moon Y.-H., Lee S.-H., Chung Y.-Y., An G. Planta 217:904-911(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INTERACTION WITH MADS16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L37527 mRNA. Translation: AAC05723.1. AC109595 Genomic DNA. Translation: AAV24770.1. AK100233 mRNA. No translation available.
PIR	T03902.
RefSeq	NP_001055590.1. NM_001062125.1.
UniGene	Os.2214.
3D structure databases
ProteinModelPortal	Q40703.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os05g34940.1; LOC_Os05g34940.1; LOC_Os05g34940.
GeneID	4338842.
KEGG	dosa:Os05t0423400-01. osa:4338842.
Organism-specific databases
Gramene	Q40703.
Phylogenomic databases
eggNOG	COG5068.
HOGENOM	HOG000155301.
ProtClustDB	CLSN2687197.
Gene expression databases
ArrayExpress	Q40703.
Family and domain databases
InterPro	IPR002487. TF_Kbox. IPR002100. TF_MADSbox. [Graphical view]
Pfam	PF01486. K-box. 1 hit. PF00319. SRF-TF. 1 hit. [Graphical view]
PRINTS	PR00404. MADSDOMAIN.
SMART	SM00432. MADS. 1 hit. [Graphical view]
SUPFAM	SSF55455. TF_MADSbox. 1 hit.
PROSITE	PS51297. K_BOX. 1 hit. PS00350. MADS_BOX_1. False negative. PS50066. MADS_BOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MADS4_ORYSJ

Accession

Primary (citable) accession number: Q40703
Secondary accession number(s): Q60EP4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	August 30, 2005
Last modified:	April 3, 2013
This is version 79 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program