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UniProtKB - Q40677 (ALFP_ORYSJ)

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Protein

Fructose-bisphosphate aldolase, chloroplastic

Gene

ALDP

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in glycolysis and gluconeogenesis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.Curated
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Os06g0256500), Glucose-6-phosphate isomerase, Glucose-6-phosphate isomerase, Glucose-6-phosphate isomerase (Os08g0478800), Glucose-6-phosphate isomerase (OsJ_27683), Glucose-6-phosphate isomerase (Os06g0256500), Glucose-6-phosphate isomerase, cytosolic A (Os03g0776000), Glucose-6-phosphate isomerase, cytosolic B (Os06g0256500), Glucose-6-phosphate isomerase, Glucose-6-phosphate isomerase (Os09g0465600)
  3. ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (Os02g0714200), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (Os09g0298100), ATP-dependent 6-phosphofructokinase (Os09g0415800), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (Os10g0405600), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (Os06g0247500), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (P0652D10.35), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (P0410E11.122), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFPA4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (Os06g0326400), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase, chloroplastic (ALDP), Fructose-bisphosphate aldolase (OsJ_21934), Fructose-bisphosphate aldolase (OsJ_00159), Fructose-bisphosphate aldolase (Os08g0120600), Fructose-bisphosphate aldolase (LOC_Os11g07020), Fructose-bisphosphate aldolase (Os06g0608700), Fructose-bisphosphate aldolase 1, cytoplasmic (FBA1), Fructose-bisphosphate aldolase (LOC_Os11g07020), Fructose-bisphosphate aldolase 2, cytoplasmic (FBA2), Fructose-bisphosphate aldolase (Os01g0118000), Fructose-bisphosphate aldolase (LOC_Os12g07210), Fructose-bisphosphate aldolase 3, cytoplasmic (FBA3)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei72SubstrateBy similarity1
Active sitei215Proton acceptorBy similarity1
Active sitei257Schiff-base intermediate with dihydroxyacetone-PBy similarity1
Binding sitei329SubstrateBy similarity1
Sitei388Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity1

GO - Molecular functioni

  • fructose-bisphosphate aldolase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  • gluconeogenesis Source: UniProtKB
  • glycolytic process Source: UniProtKB
Complete GO annotation...

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandSchiff base

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase, chloroplasticCurated (EC:4.1.2.13Curated)
Alternative name(s):
Chloroplastic aldolase1 Publication
Short name:
AldP1 Publication
Gene namesi
Name:ALDPCurated
Ordered Locus Names:Os11g0171300Imported, LOC_Os11g07020Imported
ORF Names:OsJ_33121Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza ›
Proteomesi
  • UP000059680 Componenti: Chromosome 11

Subcellular locationi

  • Plastid › chloroplast › plastoglobule By similarity

GO - Cellular componenti

  • cytoplasm Source: Gramene
  • plastoglobule Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 38Chloroplast1 PublicationAdd BLAST38
ChainiPRO_000000111239 – 388Fructose-bisphosphate aldolase, chloroplasticAdd BLAST350

Proteomic databases

PaxDbiQ40677.
PRIDEiQ40677.

Expressioni

Tissue specificityi

Expressed in leaf mesophyll cells.1 Publication

Inductioni

By exposure to light.1 Publication

Gene expression databases

ExpressionAtlasiQ40677. baseline and differential.
GenevisibleiQ40677. OS.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiQ40677. 1 interactor.
STRINGi39947.LOC_Os11g07020.1.

Structurei

3D structure databases

ProteinModelPortaliQ40677.
SMRiQ40677.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni299 – 301Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the class I fructose-bisphosphate aldolase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1557. Eukaryota.
COG3588. LUCA.
HOGENOMiHOG000220876.
InParanoidiQ40677.
KOiK01623.
OMAiLKTWQGK.
OrthoDBiEOG09360CPA.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiView protein in InterPro
IPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR000741. FBA_I.
PfamiView protein in Pfam
PF00274. Glycolytic. 1 hit.
PROSITEiView protein in PROSITE
PS00158. ALDOLASE_CLASS_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q40677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASATLLKSS FLPKKSEWGA TRQAAAPKPV TVSMVVRAGA YDDELVKTAK 
        60         70         80         90        100
TIASPGRGIL AMDESNATCG KRLASIGLEN TEANRQAYRT LLVTAPGLGQ 
       110        120        130        140        150
YISGAILFEE TLYQSTVDGK KIVDILTEQK IVPGIKVDKG LVPLAGSNNE 
       160        170        180        190        200
SWCQGLDGLA SREAAYYQQG ARFAKWRTVV SIPNGPSELA VKEAAWGLAR 
       210        220        230        240        250
YAAISQDNGL VPIVEPEILL DGEHGIDRTF EVAQKVWAET FFYMAENNVM 
       260        270        280        290        300
FEGILLKPSM VTPGAECKDR ATPEQVSDYT LKLLHRRIPP AVPGIMFLSG 
       310        320        330        340        350
GQSEVEATQN LNAMNQGPNP WHVSFSYARA LQNTCLKTWG GQPENVKAAQ 
       360        370        380 
DALLLRAKAN SLAQLGKYTS DGEAAEAKEG MFVKNYVY
Length:388
Mass (Da):42,006
Last modified:October 25, 2005 - v2
Checksum:i4FFF6FAE1F6619E0
GO

Sequence cautioni

The sequence AAX95073 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABA91631 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABA91632 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156L → R in BAA02730 (PubMed:8163192).Curated1
Sequence conflicti294G → A in BAA02730 (PubMed:8163192).Curated1
Sequence conflicti352A → R in BAA02730 (PubMed:8163192).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13513 Genomic DNA. Translation: BAA02730.1.
JX972166 mRNA. Translation: AGT38460.1.
AC128643 Genomic DNA. Translation: AAX95072.1.
AC128643 Genomic DNA. Translation: AAX95073.1. Sequence problems.
DP000010 Genomic DNA. Translation: ABA91630.2.
DP000010 Genomic DNA. Translation: ABA91631.2. Sequence problems.
DP000010 Genomic DNA. Translation: ABA91632.2. Sequence problems.
AP008217 Genomic DNA. Translation: BAF27707.1.
AP014967 Genomic DNA. Translation: BAT12868.1.
CM000148 Genomic DNA. Translation: EAZ17583.1.
AK104899 mRNA. Translation: BAG97016.1.
PIRiT03679.
RefSeqiXP_015616895.1. XM_015761409.1.
UniGeneiOs.7988.

Genome annotation databases

EnsemblPlantsiOS11T0171300-01; OS11T0171300-01; OS11G0171300.
GeneIDi4349897.
GrameneiOS11T0171300-01; OS11T0171300-01; OS11G0171300.
KEGGiosa:4349897.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiALFP_ORYSJ
AccessioniPrimary (citable) accession number: Q40677
Secondary accession number(s): Q2R9Z9
, Q2RA00, Q2RA01, Q53P95, Q53P96, U5KP25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 25, 2005
Last modified: June 7, 2017
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families