Q40677 (ALFC_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase, chloroplastic
Short name=ALDP
EC=4.1.2.13

Gene names

Ordered Locus Names:	Os11g0171300, LOC_Os11g07020
ORF Names:	OsJ_33121

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza › Oryza sativa

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway response to abscisic acid stimulus Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to cadmium ion Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	apoplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast envelope Inferred from electronic annotation. Source: EnsemblPlants/Gramene cytoplasm Inferred from sequence or structural similarity Ref.1. Source: Gramene cytosolic ribosome Inferred from electronic annotation. Source: EnsemblPlants/Gramene membrane Inferred from electronic annotation. Source: EnsemblPlants/Gramene plastoglobule Inferred from electronic annotation. Source: EnsemblPlants/Gramene thylakoid lumen Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	fructose-bisphosphate aldolase activity Inferred from sequence or structural similarity Ref.1. Source: Gramene
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 38	38	Chloroplast Ref.7
	Chain	39 – 388	350	Fructose-bisphosphate aldolase, chloroplastic		PRO_0000001112
Sites
	Active site	215	1	Proton acceptor By similarity
	Active site	257	1	Schiff-base intermediate with dihydroxyacetone-P By similarity
	Binding site	85	1	Substrate By similarity
	Binding site	175	1	Substrate By similarity
	Site	388	1	Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity
Experimental info
	Sequence conflict	156	1	L → R in BAA02730. Ref.1
	Sequence conflict	294	1	G → A in BAA02730. Ref.1
	Sequence conflict	352	1	A → R in BAA02730. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q40677 [UniParc]. Last modified October 25, 2005. Version 2. Checksum: 4FFF6FAE1F6619E0	FASTA	388	42,006
	10 20 30 40 50 60 MASATLLKSS FLPKKSEWGA TRQAAAPKPV TVSMVVRAGA YDDELVKTAK TIASPGRGIL 70 80 90 100 110 120 AMDESNATCG KRLASIGLEN TEANRQAYRT LLVTAPGLGQ YISGAILFEE TLYQSTVDGK 130 140 150 160 170 180 KIVDILTEQK IVPGIKVDKG LVPLAGSNNE SWCQGLDGLA SREAAYYQQG ARFAKWRTVV 190 200 210 220 230 240 SIPNGPSELA VKEAAWGLAR YAAISQDNGL VPIVEPEILL DGEHGIDRTF EVAQKVWAET 250 260 270 280 290 300 FFYMAENNVM FEGILLKPSM VTPGAECKDR ATPEQVSDYT LKLLHRRIPP AVPGIMFLSG 310 320 330 340 350 360 GQSEVEATQN LNAMNQGPNP WHVSFSYARA LQNTCLKTWG GQPENVKAAQ DALLLRAKAN 370 380 SLAQLGKYTS DGEAAEAKEG MFVKNYVY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of the chloroplastic and cytoplasmic aldolase-encoding genes implicated the occurrence of multiple loci in rice." Tsutsumi K., Kagaya Y., Hidaka S., Suzuki J., Tokairin Y., Hirai T., Hu D., Ishikawa K., Ejiri S. Gene 141:215-220(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[5]	"The genomes of Oryza sativa: a history of duplications." Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H. PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[6]	"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice." The rice full-length cDNA consortium Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Nipponbare.
[7]	"Proteomic analysis of rice leaf, stem and root tissues during growth course." Nozu Y., Tsugita A., Kamijo K. Proteomics 6:3665-3670(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 39-45, MASS SPECTROMETRY. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13513 Genomic DNA. Translation: BAA02730.1. AC128643 Genomic DNA. Translation: AAX95072.1. DP000010 Genomic DNA. Translation: ABA91630.2. AP008217 Genomic DNA. Translation: BAF27707.1. CM000148 Genomic DNA. Translation: EAZ17583.1. AK104899 mRNA. Translation: BAG97016.1.
PIR	T03679.
RefSeq	NP_001065862.1. NM_001072394.1.
UniGene	Os.7988.
3D structure databases
ProteinModelPortal	Q40677.
SMR	Q40677. Positions 39-372.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q40677. 1 interaction.
Proteomic databases
PaxDb	Q40677.
PRIDE	Q40677.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	OS11T0171300-01; OS11T0171300-01; OS11G0171300.
GeneID	4349897.
KEGG	osa:4349897.
Organism-specific databases
Gramene	Q40677.
Phylogenomic databases
eggNOG	COG3588.
HOGENOM	HOG000220876.
KO	K01623.
OMA	WAETFKY.
ProtClustDB	CLSN2698442.
Enzyme and pathway databases
UniPathway	UPA00109; UER00183.
Gene expression databases
ArrayExpress	Q40677.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. [Graphical view]
PANTHER	PTHR11627. PTHR11627. 1 hit.
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALFC_ORYSJ

Accession

Primary (citable) accession number: Q40677
Secondary accession number(s): Q2R9Z9, Q53P95

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	October 25, 2005
Last modified:	November 13, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program