Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Fructose-bisphosphate aldolase, chloroplastic

Gene

ALDP

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in glycolysis and gluconeogenesis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.Curated
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (Os06g0256500), Glucose-6-phosphate isomerase, Glucose-6-phosphate isomerase, Glucose-6-phosphate isomerase (Os08g0478800), Glucose-6-phosphate isomerase (OsJ_27683), Glucose-6-phosphate isomerase (Os06g0256500), Glucose-6-phosphate isomerase, cytosolic A (Os03g0776000), Glucose-6-phosphate isomerase, cytosolic B (Os06g0256500), Glucose-6-phosphate isomerase, Glucose-6-phosphate isomerase (Os09g0465600)
  3. ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (Os01g0191700), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (Os09g0298100), ATP-dependent 6-phosphofructokinase (Os09g0415800), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (Os10g0405600), ATP-dependent 6-phosphofructokinase (Os05g0524400), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (P0652D10.35), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (P0410E11.122), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFPA4), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (Os06g0326400), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase, chloroplastic (ALDP), Fructose-bisphosphate aldolase (OsJ_21934), Fructose-bisphosphate aldolase (OsJ_00159), Fructose-bisphosphate aldolase (Os08g0120600), Fructose-bisphosphate aldolase (LOC_Os11g07020), Fructose-bisphosphate aldolase (Os06g0608700), Fructose-bisphosphate aldolase 1, cytoplasmic (FBA1), Fructose-bisphosphate aldolase (LOC_Os11g07020), Fructose-bisphosphate aldolase 2, cytoplasmic (FBA2), Fructose-bisphosphate aldolase (Os01g0118000), Fructose-bisphosphate aldolase (LOC_Os12g07210), Fructose-bisphosphate aldolase 3, cytoplasmic (FBA3)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei72 – 721SubstrateBy similarity
Active sitei215 – 2151Proton acceptorBy similarity
Active sitei257 – 2571Schiff-base intermediate with dihydroxyacetone-PBy similarity
Binding sitei329 – 3291SubstrateBy similarity
Sitei388 – 3881Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

GO - Molecular functioni

  • fructose-bisphosphate aldolase activity Source: Gramene

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase, chloroplasticCurated (EC:4.1.2.13Curated)
Alternative name(s):
Chloroplastic aldolase1 Publication
Short name:
AldP1 Publication
Gene namesi
Name:ALDPCurated
Ordered Locus Names:Os11g0171300Imported, LOC_Os11g07020Imported
ORF Names:OsJ_33121Imported
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 11

Subcellular locationi

  • Plastidchloroplastplastoglobule By similarity

GO - Cellular componenti

  • chloroplast Source: UniProtKB-SubCell
  • cytoplasm Source: Gramene
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3838Chloroplast1 PublicationAdd
BLAST
Chaini39 – 388350Fructose-bisphosphate aldolase, chloroplasticPRO_0000001112Add
BLAST

Proteomic databases

PaxDbiQ40677.
PRIDEiQ40677.

Expressioni

Tissue specificityi

Expressed in leaf mesophyll cells.1 Publication

Inductioni

By exposure to light.1 Publication

Gene expression databases

ExpressionAtlasiQ40677. baseline and differential.
GenevisibleiQ40677. OS.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiQ40677. 1 interaction.
STRINGi39947.LOC_Os11g07020.1.

Structurei

3D structure databases

ProteinModelPortaliQ40677.
SMRiQ40677. Positions 39-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni299 – 3013Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1557. Eukaryota.
COG3588. LUCA.
HOGENOMiHOG000220876.
InParanoidiQ40677.
OMAiKMENNEE.
OrthoDBiEOG09360CPA.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR000741. FBA_I.
[Graphical view]
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q40677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASATLLKSS FLPKKSEWGA TRQAAAPKPV TVSMVVRAGA YDDELVKTAK
60 70 80 90 100
TIASPGRGIL AMDESNATCG KRLASIGLEN TEANRQAYRT LLVTAPGLGQ
110 120 130 140 150
YISGAILFEE TLYQSTVDGK KIVDILTEQK IVPGIKVDKG LVPLAGSNNE
160 170 180 190 200
SWCQGLDGLA SREAAYYQQG ARFAKWRTVV SIPNGPSELA VKEAAWGLAR
210 220 230 240 250
YAAISQDNGL VPIVEPEILL DGEHGIDRTF EVAQKVWAET FFYMAENNVM
260 270 280 290 300
FEGILLKPSM VTPGAECKDR ATPEQVSDYT LKLLHRRIPP AVPGIMFLSG
310 320 330 340 350
GQSEVEATQN LNAMNQGPNP WHVSFSYARA LQNTCLKTWG GQPENVKAAQ
360 370 380
DALLLRAKAN SLAQLGKYTS DGEAAEAKEG MFVKNYVY
Length:388
Mass (Da):42,006
Last modified:October 25, 2005 - v2
Checksum:i4FFF6FAE1F6619E0
GO

Sequence cautioni

The sequence AAX95073 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence ABA91631 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence ABA91632 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti156 – 1561L → R in BAA02730 (PubMed:8163192).Curated
Sequence conflicti294 – 2941G → A in BAA02730 (PubMed:8163192).Curated
Sequence conflicti352 – 3521A → R in BAA02730 (PubMed:8163192).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13513 Genomic DNA. Translation: BAA02730.1.
JX972166 mRNA. Translation: AGT38460.1.
AC128643 Genomic DNA. Translation: AAX95072.1.
AC128643 Genomic DNA. Translation: AAX95073.1. Sequence problems.
DP000010 Genomic DNA. Translation: ABA91630.2.
DP000010 Genomic DNA. Translation: ABA91631.2. Sequence problems.
DP000010 Genomic DNA. Translation: ABA91632.2. Sequence problems.
AP008217 Genomic DNA. Translation: BAF27707.1.
AP014967 Genomic DNA. Translation: BAT12868.1.
CM000148 Genomic DNA. Translation: EAZ17583.1.
AK104899 mRNA. Translation: BAG97016.1.
PIRiT03679.
RefSeqiXP_015616895.1. XM_015761409.1.
UniGeneiOs.7988.

Genome annotation databases

EnsemblPlantsiOS11T0171300-01; OS11T0171300-01; OS11G0171300.
GeneIDi4349897.
GrameneiOS11T0171300-01; OS11T0171300-01; OS11G0171300.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13513 Genomic DNA. Translation: BAA02730.1.
JX972166 mRNA. Translation: AGT38460.1.
AC128643 Genomic DNA. Translation: AAX95072.1.
AC128643 Genomic DNA. Translation: AAX95073.1. Sequence problems.
DP000010 Genomic DNA. Translation: ABA91630.2.
DP000010 Genomic DNA. Translation: ABA91631.2. Sequence problems.
DP000010 Genomic DNA. Translation: ABA91632.2. Sequence problems.
AP008217 Genomic DNA. Translation: BAF27707.1.
AP014967 Genomic DNA. Translation: BAT12868.1.
CM000148 Genomic DNA. Translation: EAZ17583.1.
AK104899 mRNA. Translation: BAG97016.1.
PIRiT03679.
RefSeqiXP_015616895.1. XM_015761409.1.
UniGeneiOs.7988.

3D structure databases

ProteinModelPortaliQ40677.
SMRiQ40677. Positions 39-372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ40677. 1 interaction.
STRINGi39947.LOC_Os11g07020.1.

Proteomic databases

PaxDbiQ40677.
PRIDEiQ40677.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS11T0171300-01; OS11T0171300-01; OS11G0171300.
GeneIDi4349897.
GrameneiOS11T0171300-01; OS11T0171300-01; OS11G0171300.

Phylogenomic databases

eggNOGiKOG1557. Eukaryota.
COG3588. LUCA.
HOGENOMiHOG000220876.
InParanoidiQ40677.
OMAiKMENNEE.
OrthoDBiEOG09360CPA.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Gene expression databases

ExpressionAtlasiQ40677. baseline and differential.
GenevisibleiQ40677. OS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR000741. FBA_I.
[Graphical view]
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALFP_ORYSJ
AccessioniPrimary (citable) accession number: Q40677
Secondary accession number(s): Q2R9Z9
, Q2RA00, Q2RA01, Q53P95, Q53P96, U5KP25
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 25, 2005
Last modified: September 7, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.