Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q40677 (ALFC_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fructose-bisphosphate aldolase, chloroplastic

Short name=ALDP
EC=4.1.2.13
Gene names
Ordered Locus Names:Os11g0171300, LOC_Os11g07020
ORF Names:OsJ_33121
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length388 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4.

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

response to cadmium ion

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentapoplast

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast envelope

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast stroma

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast thylakoid

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

cytoplasm

Inferred from sequence or structural similarity Ref.1. Source: Gramene

cytosolic ribosome

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

thylakoid lumen

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionfructose-bisphosphate aldolase activity

Inferred from sequence or structural similarity Ref.1. Source: Gramene

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3838Chloroplast Ref.7
Chain39 – 388350Fructose-bisphosphate aldolase, chloroplastic
PRO_0000001112

Sites

Active site2151Proton acceptor By similarity
Active site2571Schiff-base intermediate with dihydroxyacetone-P By similarity
Binding site851Substrate By similarity
Binding site1751Substrate By similarity
Site3881Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphate By similarity

Experimental info

Sequence conflict1561L → R in BAA02730. Ref.1
Sequence conflict2941G → A in BAA02730. Ref.1
Sequence conflict3521A → R in BAA02730. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q40677 [UniParc].

Last modified October 25, 2005. Version 2.
Checksum: 4FFF6FAE1F6619E0

FASTA38842,006
        10         20         30         40         50         60 
MASATLLKSS FLPKKSEWGA TRQAAAPKPV TVSMVVRAGA YDDELVKTAK TIASPGRGIL 

        70         80         90        100        110        120 
AMDESNATCG KRLASIGLEN TEANRQAYRT LLVTAPGLGQ YISGAILFEE TLYQSTVDGK 

       130        140        150        160        170        180 
KIVDILTEQK IVPGIKVDKG LVPLAGSNNE SWCQGLDGLA SREAAYYQQG ARFAKWRTVV 

       190        200        210        220        230        240 
SIPNGPSELA VKEAAWGLAR YAAISQDNGL VPIVEPEILL DGEHGIDRTF EVAQKVWAET 

       250        260        270        280        290        300 
FFYMAENNVM FEGILLKPSM VTPGAECKDR ATPEQVSDYT LKLLHRRIPP AVPGIMFLSG 

       310        320        330        340        350        360 
GQSEVEATQN LNAMNQGPNP WHVSFSYARA LQNTCLKTWG GQPENVKAAQ DALLLRAKAN 

       370        380 
SLAQLGKYTS DGEAAEAKEG MFVKNYVY 

« Hide

References

« Hide 'large scale' references
[1]"Structural analysis of the chloroplastic and cytoplasmic aldolase-encoding genes implicated the occurrence of multiple loci in rice."
Tsutsumi K., Kagaya Y., Hidaka S., Suzuki J., Tokairin Y., Hirai T., Hu D., Ishikawa K., Ejiri S.
Gene 141:215-220(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
The rice chromosomes 11 and 12 sequencing consortia
BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[4]"The rice annotation project database (RAP-DB): 2008 update."
The rice annotation project (RAP)
Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[5]"The genomes of Oryza sativa: a history of duplications."
Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L. expand/collapse author list , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[6]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[7]"Proteomic analysis of rice leaf, stem and root tissues during growth course."
Nozu Y., Tsugita A., Kamijo K.
Proteomics 6:3665-3670(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 39-45, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D13513 Genomic DNA. Translation: BAA02730.1.
AC128643 Genomic DNA. Translation: AAX95072.1.
DP000010 Genomic DNA. Translation: ABA91630.2.
AP008217 Genomic DNA. Translation: BAF27707.1.
CM000148 Genomic DNA. Translation: EAZ17583.1.
AK104899 mRNA. Translation: BAG97016.1.
PIRT03679.
RefSeqNP_001065862.1. NM_001072394.1.
UniGeneOs.7988.

3D structure databases

ProteinModelPortalQ40677.
SMRQ40677. Positions 39-372.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ40677. 1 interaction.

Proteomic databases

PaxDbQ40677.
PRIDEQ40677.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS11T0171300-01; OS11T0171300-01; OS11G0171300.
GeneID4349897.
KEGGosa:4349897.

Organism-specific databases

GrameneQ40677.

Phylogenomic databases

eggNOGCOG3588.
HOGENOMHOG000220876.
KOK01623.
OMAVEPEIML.

Enzyme and pathway databases

UniPathwayUPA00109; UER00183.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR000741. Aldolase_I.
IPR013785. Aldolase_TIM.
[Graphical view]
PANTHERPTHR11627. PTHR11627. 1 hit.
PfamPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALFC_ORYSJ
AccessionPrimary (citable) accession number: Q40677
Secondary accession number(s): Q2R9Z9, Q53P95
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 25, 2005
Last modified: June 11, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways