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Protein

Fructose-bisphosphate aldolase, chloroplastic

Gene

Os11g0171300

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851SubstrateBy similarity
Binding sitei175 – 1751SubstrateBy similarity
Active sitei215 – 2151Proton acceptorBy similarity
Active sitei257 – 2571Schiff-base intermediate with dihydroxyacetone-PBy similarity
Sitei388 – 3881Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: Gramene

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase, chloroplastic (EC:4.1.2.13)
Short name:
ALDP
Gene namesi
Ordered Locus Names:Os11g0171300, LOC_Os11g07020
ORF Names:OsJ_33121
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763 Componenti: Chromosome 11

Organism-specific databases

GrameneiQ40677.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
  2. cytoplasm Source: Gramene
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3838Chloroplast1 PublicationAdd
BLAST
Chaini39 – 388350Fructose-bisphosphate aldolase, chloroplasticPRO_0000001112Add
BLAST

Proteomic databases

PaxDbiQ40677.
PRIDEiQ40677.

Expressioni

Gene expression databases

ExpressionAtlasiQ40677. baseline.

Interactioni

Protein-protein interaction databases

IntActiQ40677. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ40677.
SMRiQ40677. Positions 39-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG3588.
HOGENOMiHOG000220876.
InParanoidiQ40677.
KOiK01623.
OMAiRTFEIAQ.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q40677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASATLLKSS FLPKKSEWGA TRQAAAPKPV TVSMVVRAGA YDDELVKTAK
60 70 80 90 100
TIASPGRGIL AMDESNATCG KRLASIGLEN TEANRQAYRT LLVTAPGLGQ
110 120 130 140 150
YISGAILFEE TLYQSTVDGK KIVDILTEQK IVPGIKVDKG LVPLAGSNNE
160 170 180 190 200
SWCQGLDGLA SREAAYYQQG ARFAKWRTVV SIPNGPSELA VKEAAWGLAR
210 220 230 240 250
YAAISQDNGL VPIVEPEILL DGEHGIDRTF EVAQKVWAET FFYMAENNVM
260 270 280 290 300
FEGILLKPSM VTPGAECKDR ATPEQVSDYT LKLLHRRIPP AVPGIMFLSG
310 320 330 340 350
GQSEVEATQN LNAMNQGPNP WHVSFSYARA LQNTCLKTWG GQPENVKAAQ
360 370 380
DALLLRAKAN SLAQLGKYTS DGEAAEAKEG MFVKNYVY
Length:388
Mass (Da):42,006
Last modified:October 25, 2005 - v2
Checksum:i4FFF6FAE1F6619E0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti156 – 1561L → R in BAA02730 (PubMed:8163192).Curated
Sequence conflicti294 – 2941G → A in BAA02730 (PubMed:8163192).Curated
Sequence conflicti352 – 3521A → R in BAA02730 (PubMed:8163192).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13513 Genomic DNA. Translation: BAA02730.1.
AC128643 Genomic DNA. Translation: AAX95072.1.
DP000010 Genomic DNA. Translation: ABA91630.2.
AP008217 Genomic DNA. Translation: BAF27707.1.
CM000148 Genomic DNA. Translation: EAZ17583.1.
AK104899 mRNA. Translation: BAG97016.1.
PIRiT03679.
RefSeqiNP_001065862.1. NM_001072394.1.
UniGeneiOs.7988.

Genome annotation databases

EnsemblPlantsiOS11T0171300-01; OS11T0171300-01; OS11G0171300.
GeneIDi4349897.
KEGGiosa:4349897.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D13513 Genomic DNA. Translation: BAA02730.1.
AC128643 Genomic DNA. Translation: AAX95072.1.
DP000010 Genomic DNA. Translation: ABA91630.2.
AP008217 Genomic DNA. Translation: BAF27707.1.
CM000148 Genomic DNA. Translation: EAZ17583.1.
AK104899 mRNA. Translation: BAG97016.1.
PIRiT03679.
RefSeqiNP_001065862.1. NM_001072394.1.
UniGeneiOs.7988.

3D structure databases

ProteinModelPortaliQ40677.
SMRiQ40677. Positions 39-372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ40677. 1 interaction.

Proteomic databases

PaxDbiQ40677.
PRIDEiQ40677.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS11T0171300-01; OS11T0171300-01; OS11G0171300.
GeneIDi4349897.
KEGGiosa:4349897.

Organism-specific databases

GrameneiQ40677.

Phylogenomic databases

eggNOGiCOG3588.
HOGENOMiHOG000220876.
InParanoidiQ40677.
KOiK01623.
OMAiRTFEIAQ.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Gene expression databases

ExpressionAtlasiQ40677. baseline.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR029768. Aldolase_I_AS.
IPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
PROSITEiPS00158. ALDOLASE_CLASS_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structural analysis of the chloroplastic and cytoplasmic aldolase-encoding genes implicated the occurrence of multiple loci in rice."
    Tsutsumi K., Kagaya Y., Hidaka S., Suzuki J., Tokairin Y., Hirai T., Hu D., Ishikawa K., Ejiri S.
    Gene 141:215-220(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
    The rice chromosomes 11 and 12 sequencing consortia
    BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  6. "Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
    The rice full-length cDNA consortium
    Science 301:376-379(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Nipponbare.
  7. "Proteomic analysis of rice leaf, stem and root tissues during growth course."
    Nozu Y., Tsugita A., Kamijo K.
    Proteomics 6:3665-3670(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 39-45, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiALFC_ORYSJ
AccessioniPrimary (citable) accession number: Q40677
Secondary accession number(s): Q2R9Z9, Q53P95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: October 25, 2005
Last modified: April 1, 2015
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.