Reviewed, UniProtKB/Swiss-Prot Q40637 (EXPA3_ORYSJ)

Names and origin

Protein names

Recommended name:
    Expansin-A3
Alternative name(s):
    OsEXPA3
    Alpha-expansin-3
    OsEXP3
    OsaEXPa1.18
    RiExD

Gene names

Name:	EXPA3
Synonyms:	EXP3
Ordered Locus Names:	Os05g0276500, LOC_Os05g19570

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May cause loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found. May be required for rapid internodal elongation in deepwater rice during submergence By similarity.
Subcellular location	Secreted › cell wall By similarity. Membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expressed in the epidermis and vascular cylinder of the root tip, lateral root primordia, adventitious root primordia, shoot apex and leaf primordia. Ref.3 Ref.4
Developmental stage	Expressed in the apical region (growing zone) of the root hair. Ref.3
Sequence similarities	Belongs to the expansin family. Expansin A subfamily. Contains 1 expansin-like CBD domain. Contains 1 expansin-like EG45 domain.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Membrane Secreted
Domain	Signal
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	plant-type cell wall organization Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 255	227	Expansin-A3		PRO_0000251982
Regions
	Domain	46 – 161	116	Expansin-like EG45
	Domain	171 – 250	80	Expansin-like CBD
Amino acid modifications
	Disulfide bond	49 ↔ 77		By similarity
	Disulfide bond	80 ↔ 156		By similarity
	Disulfide bond	85 ↔ 93		By similarity
Experimental info
	Sequence conflict	43	1	G → C in AAB38075. Ref.1
	Sequence conflict	126	1	R → L in AAB38075. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q40637-1 [UniParc]. Last modified October 3, 2006. Version 2. Checksum: C5809DFDC93C8038	FASTA	255	27,305
	10 20 30 40 50 60 MLSGMEKQPA MLLVLVTLCA FACKRSVAQS AFATFYGGKD GSGTMGGACG YGNLYNAGYG 70 80 90 100 110 120 LYNAALSSAL FNDGAMCGAC YTITCDTSQT KWCKPGGNSI TITATNLCPP NWALPSNSGG 130 140 150 160 170 180 WCNPPRQHFD MSQPAWENIA VYQAGIVPVN YKRVPCQRSG GIRFAISGHD YFELVTVTNV 190 200 210 220 230 240 GGSGVVAQMS IKGSNTGWMA MSRNWGANWQ SNAYLAGQSL SFIVQLDDGR KVTAWNVAPS 250 NWFFGATYST SWVQF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequence analysis of expansins - a highly conserved, multigene family of proteins that mediate cell wall extension in plants." Shcherban T.Y., Shi J., Durachko D.M., Guiltinan M.J., McQueen-Mason S.J., Shieh M., Cosgrove D.J. Proc. Natl. Acad. Sci. U.S.A. 92:9245-9249(1995) [PubMed: 7568110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed: 16261349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Expression of expansin genes is correlated with growth in deepwater rice." Cho H.-T., Kende H. Plant Cell 9:1661-1671(1997) [PubMed: 9338967] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. Nipponbare. Tissue: Shoot.
[4]	"Tissue localization of expansins in deepwater rice." Cho H.-T., Kende H. Plant J. 15:805-812(1998) [PubMed: 9807819] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Nomenclature for members of the expansin superfamily of genes and proteins." Kende H., Bradford K.J., Brummell D.A., Cho H.-T., Cosgrove D.J., Fleming A.J., Gehring C., Lee Y., McQueen-Mason S.J., Rose J.K.C., Voesenek L.A.C. Plant Mol. Biol. 55:311-314(2004) [PubMed: 15604683] [Abstract] Cited for: NOMENCLATURE.

Web resources

EXPANSIN homepage

Cross-references

Sequence databases
EMBL GenBank DDBJ	U30479 mRNA. Translation: AAB38075.1. AC130731 Genomic DNA. No translation available.
PIR	T03299.
RefSeq	NP_001055077.1.
UniGene	Os.410
3D structure databases
SMR	Q40637. Positions 28-255.
ModBase	Search...
Genome annotation databases
GeneID	4338278.
KEGG	osa:4338278.
Organism-specific databases
Gramene	Q40637.
Family and domain databases
InterPro	IPR009009. Barwin-related_endoglucanase. IPR007112. Expan_endogl. IPR007118. Expan_Lol_pI. IPR007117. Expan_Lol_pI_C. IPR002963. Expansin. IPR014734. Pollen_allergen. IPR005132. RlpA. [Graphical view]
Gene3D	G3DSA:2.60.40.760. Expan_Lol_pI_C. 1 hit. G3DSA:2.40.40.40. Pollen_allergen. 1 hit.
Pfam	PF03330. DPBB_1. 1 hit. PF01357. Pollen_allerg_1. 1 hit. [Graphical view]
PRINTS	PR01226. EXPANSIN. PR01225. EXPANSNFAMLY.
PROSITE	PS50843. EXPANSIN_CBD. 1 hit. PS50842. EXPANSIN_EG45. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EXPA3_ORYSJ

Accession

Primary (citable) accession number: Q40637

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	October 3, 2006
Last modified:	January 19, 2010
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)