Reviewed, UniProtKB/Swiss-Prot Q40636 (EXPA2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Expansin-A2
Alternative name(s):
    OsEXPA2
    Alpha-expansin-2
    OsEXP2
    OsaEXPa1.23
    RiExB
    RiExC

Gene names

Name:	EXPA2
Synonyms:	EXP2
Ordered Locus Names:	Os01g0823100, LOC_Os01g60770
ORF Names:	P0031D02.10, P0485B12.42

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	May cause loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found. May be required for rapid internodal elongation in deepwater rice during submergence By similarity.
Subcellular location	Secreted › cell wall By similarity. Membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expressed in lateral root primordia, adventitious root primordia, coleoptiles, shoot apex, leaf primordia, panicles and flowers. Ref.6 Ref.7 Ref.10
Developmental stage	Expressed in the apical region (growing zone) of the root hair. Expressed in the growing region of leaves. Expressed in developing seeds. Ref.6 Ref.2 Ref.8
Induction	By gibberellin (GA3), submergence and wounding. Ref.6 Ref.2 Ref.8
Sequence similarities	Belongs to the expansin family. Expansin A subfamily. Contains 1 expansin-like CBD domain. Contains 1 expansin-like EG45 domain.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Membrane Secreted
Domain	Signal
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	plant-type cell wall organization Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 251	227	Expansin-A2		PRO_0000251981
Regions
	Domain	47 – 159	113	Expansin-like EG45
	Domain	169 – 248	80	Expansin-like CBD
Amino acid modifications
	Disulfide bond	50 ↔ 78		By similarity
	Disulfide bond	81 ↔ 154		By similarity
	Disulfide bond	86 ↔ 93		By similarity
Experimental info
	Sequence conflict	79	1	G → R in AAB38074. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q40636-1 [UniParc]. Last modified October 3, 2006. Version 2. Checksum: C450366570EFE3BD	FASTA	251	26,674
	10 20 30 40 50 60 MASRSSALLL LFSAFCFLAR RAAADYGSWQ SAHATFYGGG DASGTMGGAC GYGNLYSTGY 70 80 90 100 110 120 GTNTAALSTV LFNDGAACGS CYELRCDNDG QWCLPGSVTV TATNLCPPNY ALPNDDGGWC 130 140 150 160 170 180 NPPRPHFDMA EPAFLQIGVY RAGIVPVSYR RVPCVKKGGI RFTINGHSYF NLVLVTNVAG 190 200 210 220 230 240 PGDVQSVSIK GSSTGWQPMS RNWGQNWQSN SYLDGQSLSF QVAVSDGRTV TSNNVVPAGW 250 QFGQTFEGGQ F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and sequence analysis of expansins - a highly conserved, multigene family of proteins that mediate cell wall extension in plants." Shcherban T.Y., Shi J., Durachko D.M., Guiltinan M.J., McQueen-Mason S.J., Shieh M., Cosgrove D.J. Proc. Natl. Acad. Sci. U.S.A. 92:9245-9249(1995) [PubMed: 7568110] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"Expression of alpha-expansin and expansin-like genes in deepwater rice." Lee Y., Kende H. Plant Physiol. 130:1396-1405(2002) [PubMed: 12428004] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, INDUCTION.
[3]	"The genome sequence and structure of rice chromosome 1." Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y., Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H., Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M. , Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M., Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T., Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S., Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H., Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y., Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S., Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y., Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S., Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H., Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T. Nature 420:312-316(2002) [PubMed: 12447438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[4]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[5]	"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana." The rice annotation project (RAP) Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
[6]	"Expression of expansin genes is correlated with growth in deepwater rice." Cho H.-T., Kende H. Plant Cell 9:1661-1671(1997) [PubMed: 9338967] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION. Strain: cv. Nipponbare. Tissue: Shoot.
[7]	"Tissue localization of expansins in deepwater rice." Cho H.-T., Kende H. Plant J. 15:805-812(1998) [PubMed: 9807819] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Expression of alpha-expansin genes in young seedlings of rice (Oryza sativa L.)." Huang J., Takano T., Akita S. Planta 211:467-473(2000) [PubMed: 11030545] [Abstract] Cited for: DEVELOPMENTAL STAGE, INDUCTION.
[9]	"Nomenclature for members of the expansin superfamily of genes and proteins." Kende H., Bradford K.J., Brummell D.A., Cho H.-T., Cosgrove D.J., Fleming A.J., Gehring C., Lee Y., McQueen-Mason S.J., Rose J.K.C., Voesenek L.A.C. Plant Mol. Biol. 55:311-314(2004) [PubMed: 15604683] [Abstract] Cited for: NOMENCLATURE.
[10]	"Characterization and transcriptional expression of the alpha-expansin gene family in rice." Shin J.-H., Jeong D.-H., Park M.C., An G. Mol. Cells 20:210-218(2005) [PubMed: 16267395] [Abstract] Cited for: TISSUE SPECIFICITY.

Web resources

EXPANSIN homepage

Cross-references

Sequence databases
EMBL GenBank DDBJ	U30477 mRNA. Translation: AAB38074.1. AF394544 Genomic DNA. Translation: AAL24480.1. AP003230 Genomic DNA. Translation: BAB93180.1. AP003348 Genomic DNA. Translation: BAB86504.1. AP008207 Genomic DNA. Translation: BAF06570.1.
PIR	T03298.
RefSeq	NP_001044656.1.
UniGene	Os.4644
3D structure databases
SMR	Q40636. Positions 23-251.
ModBase	Search...
Genome annotation databases
GeneID	4327568.
KEGG	osa:4327568.
NMPDR	fig|39947.1.peg.7336.
Organism-specific databases
Gramene	Q40636.
Phylogenomic databases
HOGENOM	HBG605129.
Family and domain databases
InterPro	IPR009009. Barwin-related_endoglucanase. IPR007112. Expan_endogl. IPR007118. Expan_Lol_pI. IPR007117. Expan_Lol_pI_C. IPR002963. Expansin. IPR014734. Pollen_allergen. IPR005132. RlpA. [Graphical view]
Gene3D	G3DSA:2.60.40.760. Expan_Lol_pI_C. 1 hit. G3DSA:2.40.40.40. Pollen_allergen. 1 hit.
Pfam	PF03330. DPBB_1. 1 hit. PF01357. Pollen_allerg_1. 1 hit. [Graphical view]
PRINTS	PR01226. EXPANSIN. PR01225. EXPANSNFAMLY.
PROSITE	PS50843. EXPANSIN_CBD. 1 hit. PS50842. EXPANSIN_EG45. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EXPA2_ORYSJ

Accession

Primary (citable) accession number: Q40636
Secondary accession number(s): Q7F5X8, Q946J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
Last sequence update:	October 3, 2006
Last modified:	February 9, 2010
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)