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Q40577

- 5EAS_TOBAC

UniProt

Q40577 - 5EAS_TOBAC

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Protein

5-epi-aristolochene synthase

Gene
EAS3
EAS4
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the cyclization of trans,trans-farnesyl diphosphate (FPP) to the bicyclic intermediate 5-epi-aristolochene, initial step in the conversion of FPP to the sesquiterpenoid antifungal phytoalexin capsidiol. Produces germacrene A as an enzyme-bound intermediate that is not released by the enzyme, but is further cyclized to produce the bicyclic 5-epi-aristolochene.

Catalytic activityi

(2E,6E)-farnesyl diphosphate = (+)-5-epiaristolochene + diphosphate.1 Publication

Cofactori

Binds 3 magnesium ions per subunit.

Kineticsi

  1. KM=2.3 µM for 2-trans,6-trans-farnesyl diphosphate1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi301 – 3011Magnesium 1
Metal bindingi301 – 3011Magnesium 2
Metal bindingi305 – 3051Magnesium 1
Metal bindingi305 – 3051Magnesium 2
Metal bindingi444 – 4441Magnesium 3
Metal bindingi448 – 4481Magnesium 3
Metal bindingi452 – 4521Magnesium 3

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. terpene synthase activity Source: InterPro

GO - Biological processi

  1. terpenoid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:EAS-MONOMER.
BRENDAi4.2.3.9. 3645.
UniPathwayiUPA00213.

Names & Taxonomyi

Protein namesi
Recommended name:
5-epi-aristolochene synthase (EC:4.2.3.61)
Short name:
EAS
Gene namesi
Name:EAS3
AND
Name:EAS4
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi520 – 5201Y → F: Loss of production of aristolochene, and accumulation of the intermediate germacrene A. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5485485-epi-aristolochene synthasePRO_0000186438Add
BLAST

Expressioni

Inductioni

By fungal elicitor.1 Publication

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1
548
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni24 – 296
Helixi36 – 5722
Helixi64 – 7613
Helixi80 – 823
Helixi84 – 9714
Helixi104 – 11613
Helixi123 – 1297
Beta strandi134 – 1363
Helixi138 – 1425
Helixi144 – 15411
Helixi162 – 1643
Helixi167 – 17812
Helixi179 – 1813
Helixi186 – 19510
Helixi203 – 21311
Helixi215 – 2173
Helixi223 – 25230
Turni253 – 2553
Helixi256 – 2594
Helixi267 – 27711
Helixi281 – 2833
Helixi284 – 30522
Helixi310 – 32213
Helixi325 – 3306
Helixi333 – 35422
Turni355 – 3573
Helixi359 – 3613
Helixi362 – 38524
Helixi391 – 3988
Helixi400 – 4023
Helixi404 – 41310
Helixi420 – 4278
Helixi431 – 44717
Helixi449 – 4546
Helixi461 – 4699
Helixi473 – 49422
Beta strandi496 – 4983
Helixi503 – 5053
Helixi507 – 51913
Turni520 – 5223
Turni523 – 5253
Turni526 – 5283
Helixi531 – 54212

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1HX9X-ray3.50A1-548[»]
1HXAX-ray2.32A1-548[»]
1HXCX-ray2.25A1-548[»]
1HXGX-ray2.90A1-548[»]
3LZ9X-ray2.28A1-548[»]
3M00X-ray2.10A1-548[»]
3M01X-ray2.60A1-548[»]
3M02X-ray2.50A1-548[»]
4DI5X-ray2.30A14-548[»]
5EASX-ray2.25A1-548[»]
5EATX-ray2.80A1-548[»]
5EAUX-ray2.15A1-548[»]
ProteinModelPortaliQ40577.
SMRiQ40577. Positions 21-548.

Miscellaneous databases

EvolutionaryTraceiQ40577.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi301 – 3055DDXXD motif

Domaini

The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

Sequence similaritiesi

Belongs to the terpene synthase family.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Q40577-1 [UniParc]FASTAAdd to Basket

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MASAAVANYE EEIVRPVADF SPSLWGDQFL SFSIKNQVAE KYAKEIEALK    50
EQTRNMLLAT GMKLADTLNL IDTIERLGIS YHFEKEIDDI LDQIYNQNSN 100
CNDLCTSALQ FRLLRQHGFN ISPEIFSKFQ DENGKFKESL ASDVLGLLNL 150
YEASHVRTHA DDILEDALAF STIHLESAAP HLKSPLREQV THALEQCLHK 200
GVPRVETRFF ISSIYDKEQS KNNVLLRFAK LDFNLLQMLH KQELAQVSRW 250
WKDLDFVTTL PYARDRVVEC YFWALGVYFE PQYSQARVML VKTISMISIV 300
DDTFDAYGTV KELEAYTDAI QRWDINEIDR LPDYMKISYK AILDLYKDYE 350
KELSSAGRSH IVCHAIERMK EVVRNYNVES TWFIEGYTPP VSEYLSNALA 400
TTTYYYLATT SYLGMKSATE QDFEWLSKNP KILEASVIIC RVIDDTATYE 450
VEKSRGQIAT GIECCMRDYG ISTKEAMAKF QNMAETAWKD INEGLLRPTP 500
VSTEFLTPIL NLARIVEVTY IHNLDGYTHP EKVLKPHIIN LLVDSIKI 548
Length:548
Mass (Da):62,987
Last modified:April 29, 2008 - v3
Checksum:i9FE25FF361A68BF1
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti35 – 351K → D in AAA19216. 1 Publication
Sequence conflicti42 – 421Y → YIY AA sequence 1 Publication
Sequence conflicti44 – 441K → Q in AAA19216. 1 Publication
Sequence conflicti55 – 551N → S in AAA19216. 1 Publication
Sequence conflicti62 – 621M → R in AAA19216. 1 Publication
Sequence conflicti73 – 731T → I in AAA19216. 1 Publication
Sequence conflicti89 – 891D → E in AAA19216. 1 Publication
Sequence conflicti388 – 3881T → M in AAA19216. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04680 Unassigned RNA. Translation: AAA19216.1.
PIRiT03714.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L04680 Unassigned RNA. Translation: AAA19216.1 .
PIRi T03714.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1HX9 X-ray 3.50 A 1-548 [» ]
1HXA X-ray 2.32 A 1-548 [» ]
1HXC X-ray 2.25 A 1-548 [» ]
1HXG X-ray 2.90 A 1-548 [» ]
3LZ9 X-ray 2.28 A 1-548 [» ]
3M00 X-ray 2.10 A 1-548 [» ]
3M01 X-ray 2.60 A 1-548 [» ]
3M02 X-ray 2.50 A 1-548 [» ]
4DI5 X-ray 2.30 A 14-548 [» ]
5EAS X-ray 2.25 A 1-548 [» ]
5EAT X-ray 2.80 A 1-548 [» ]
5EAU X-ray 2.15 A 1-548 [» ]
ProteinModelPortali Q40577.
SMRi Q40577. Positions 21-548.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00213 .
BioCyci MetaCyc:EAS-MONOMER.
BRENDAi 4.2.3.9. 3645.

Miscellaneous databases

EvolutionaryTracei Q40577.

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProi IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view ]
SUPFAMi SSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Gene family for an elicitor-induced sesquiterpene cyclase in tobacco."
    Facchini P.J., Chappell J.
    Proc. Natl. Acad. Sci. U.S.A. 89:11088-11092(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 56-73, INDUCTION.
    Strain: cv. NK 326.
  2. O'Maille P.E.
    Unpublished observations (APR-2008)
    Cited for: SEQUENCE REVISION TO 35.
  3. "Demonstration of germacrene A as an intermediate in 5-epi-aristolochene synthase catalysis."
    Rising K.A., Starks C.M., Noel J.P., Chappell J.
    J. Am. Chem. Soc. 122:1861-1866(2000)
    Cited for: CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, MUTAGENESIS OF TYR-520.
  4. "Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase."
    Starks C.M., Back K., Chappell J., Noel J.P.
    Science 277:1815-1820(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) IN COMPLEX WITH MAGNESIUM IONS AND SUBSTRATE, SEQUENCE REVISION.

Entry informationi

Entry namei5EAS_TOBAC
AccessioniPrimary (citable) accession number: Q40577
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: April 29, 2008
Last modified: February 19, 2014
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

1 Publication indicates the presence of at least two genes coding for the same protein.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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