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Protein

5-epi-aristolochene synthase

Gene

EAS3

more
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cyclization of trans,trans-farnesyl diphosphate (FPP) to the bicyclic intermediate 5-epi-aristolochene, initial step in the conversion of FPP to the sesquiterpenoid antifungal phytoalexin capsidiol. Produces germacrene A as an enzyme-bound intermediate that is not released by the enzyme, but is further cyclized to produce the bicyclic 5-epi-aristolochene.

Catalytic activityi

(2E,6E)-farnesyl diphosphate = (+)-5-epiaristolochene + diphosphate.1 Publication

Cofactori

Mg2+1 PublicationNote: Binds 3 Mg2+ ions per subunit.1 Publication

Kineticsi

  1. KM=2.3 µM for 2-trans,6-trans-farnesyl diphosphate1 Publication

    Pathwayi: terpenoid biosynthesis

    This protein is involved in the pathway terpenoid biosynthesis, which is part of Secondary metabolite biosynthesis.
    View all proteins of this organism that are known to be involved in the pathway terpenoid biosynthesis and in Secondary metabolite biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi301Magnesium 11 Publication1
    Metal bindingi301Magnesium 21 Publication1
    Metal bindingi305Magnesium 11 Publication1
    Metal bindingi305Magnesium 21 Publication1
    Metal bindingi444Magnesium 31 Publication1
    Metal bindingi448Magnesium 31 Publication1
    Metal bindingi452Magnesium 31 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:EAS-MONOMER.
    BRENDAi4.2.3.61. 3645.
    UniPathwayiUPA00213.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-epi-aristolochene synthase (EC:4.2.3.61)
    Short name:
    EAS
    Gene namesi
    Name:EAS3
    AND
    Name:EAS4
    OrganismiNicotiana tabacum (Common tobacco)
    Taxonomic identifieri4097 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi520Y → F: Loss of production of aristolochene, and accumulation of the intermediate germacrene A. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001864381 – 5485-epi-aristolochene synthaseAdd BLAST548

    Expressioni

    Inductioni

    By fungal elicitor.1 Publication

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Structurei

    Secondary structure

    1548
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Turni24 – 29Combined sources6
    Helixi36 – 57Combined sources22
    Helixi64 – 76Combined sources13
    Helixi80 – 82Combined sources3
    Helixi84 – 97Combined sources14
    Helixi104 – 116Combined sources13
    Helixi123 – 129Combined sources7
    Beta strandi134 – 136Combined sources3
    Helixi138 – 142Combined sources5
    Helixi144 – 154Combined sources11
    Helixi162 – 164Combined sources3
    Helixi167 – 178Combined sources12
    Helixi179 – 181Combined sources3
    Helixi186 – 195Combined sources10
    Helixi203 – 213Combined sources11
    Helixi215 – 217Combined sources3
    Helixi223 – 252Combined sources30
    Turni253 – 255Combined sources3
    Helixi256 – 259Combined sources4
    Helixi267 – 277Combined sources11
    Helixi281 – 283Combined sources3
    Helixi284 – 305Combined sources22
    Helixi310 – 322Combined sources13
    Helixi325 – 330Combined sources6
    Helixi333 – 354Combined sources22
    Turni355 – 357Combined sources3
    Helixi359 – 361Combined sources3
    Helixi362 – 385Combined sources24
    Helixi391 – 398Combined sources8
    Helixi400 – 402Combined sources3
    Helixi404 – 413Combined sources10
    Helixi420 – 427Combined sources8
    Helixi431 – 447Combined sources17
    Helixi449 – 454Combined sources6
    Helixi461 – 469Combined sources9
    Helixi473 – 494Combined sources22
    Beta strandi496 – 498Combined sources3
    Helixi503 – 505Combined sources3
    Helixi507 – 519Combined sources13
    Turni520 – 522Combined sources3
    Turni523 – 525Combined sources3
    Turni526 – 528Combined sources3
    Helixi531 – 542Combined sources12

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1HX9X-ray3.50A1-548[»]
    1HXAX-ray2.32A1-548[»]
    1HXCX-ray2.25A1-548[»]
    1HXGX-ray2.90A1-548[»]
    3LZ9X-ray2.28A1-548[»]
    3M00X-ray2.10A1-548[»]
    3M01X-ray2.60A1-548[»]
    3M02X-ray2.50A1-548[»]
    4DI5X-ray2.30A14-548[»]
    4RNQX-ray2.47A1-548[»]
    5DHIX-ray2.25A13-548[»]
    5DHKX-ray2.43A13-548[»]
    5EASX-ray2.25A1-548[»]
    5EATX-ray2.80A1-548[»]
    5EAUX-ray2.15A1-548[»]
    5IK0X-ray2.20A1-548[»]
    5IK6X-ray2.30A1-548[»]
    5IK9X-ray2.23A1-548[»]
    5IKAX-ray2.45A1-548[»]
    5IKHX-ray2.10A1-548[»]
    ProteinModelPortaliQ40577.
    SMRiQ40577.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ40577.

    Family & Domainsi

    Motif

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Motifi301 – 305DDXXD motif5

    Domaini

    The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.

    Sequence similaritiesi

    Belongs to the terpene synthase family.Curated

    Phylogenomic databases

    KOiK15804.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    1.50.30.10. 1 hit.
    InterProiIPR008949. Isoprenoid_synthase_dom.
    IPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view]
    PfamiPF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 1 hit.
    SSF48576. SSF48576. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q40577-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MASAAVANYE EEIVRPVADF SPSLWGDQFL SFSIKNQVAE KYAKEIEALK
    60 70 80 90 100
    EQTRNMLLAT GMKLADTLNL IDTIERLGIS YHFEKEIDDI LDQIYNQNSN
    110 120 130 140 150
    CNDLCTSALQ FRLLRQHGFN ISPEIFSKFQ DENGKFKESL ASDVLGLLNL
    160 170 180 190 200
    YEASHVRTHA DDILEDALAF STIHLESAAP HLKSPLREQV THALEQCLHK
    210 220 230 240 250
    GVPRVETRFF ISSIYDKEQS KNNVLLRFAK LDFNLLQMLH KQELAQVSRW
    260 270 280 290 300
    WKDLDFVTTL PYARDRVVEC YFWALGVYFE PQYSQARVML VKTISMISIV
    310 320 330 340 350
    DDTFDAYGTV KELEAYTDAI QRWDINEIDR LPDYMKISYK AILDLYKDYE
    360 370 380 390 400
    KELSSAGRSH IVCHAIERMK EVVRNYNVES TWFIEGYTPP VSEYLSNALA
    410 420 430 440 450
    TTTYYYLATT SYLGMKSATE QDFEWLSKNP KILEASVIIC RVIDDTATYE
    460 470 480 490 500
    VEKSRGQIAT GIECCMRDYG ISTKEAMAKF QNMAETAWKD INEGLLRPTP
    510 520 530 540
    VSTEFLTPIL NLARIVEVTY IHNLDGYTHP EKVLKPHIIN LLVDSIKI
    Length:548
    Mass (Da):62,987
    Last modified:April 29, 2008 - v3
    Checksum:i9FE25FF361A68BF1
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti35K → D in AAA19216 (PubMed:1438319).Curated1
    Sequence conflicti42Y → YIY AA sequence (PubMed:1438319).Curated1
    Sequence conflicti44K → Q in AAA19216 (PubMed:1438319).Curated1
    Sequence conflicti55N → S in AAA19216 (PubMed:1438319).Curated1
    Sequence conflicti62M → R in AAA19216 (PubMed:1438319).Curated1
    Sequence conflicti73T → I in AAA19216 (PubMed:1438319).Curated1
    Sequence conflicti89D → E in AAA19216 (PubMed:1438319).Curated1
    Sequence conflicti388T → M in AAA19216 (PubMed:1438319).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L04680 Unassigned RNA. Translation: AAA19216.1.
    PIRiT03714.

    Genome annotation databases

    KEGGiag:AAA19216.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L04680 Unassigned RNA. Translation: AAA19216.1.
    PIRiT03714.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1HX9X-ray3.50A1-548[»]
    1HXAX-ray2.32A1-548[»]
    1HXCX-ray2.25A1-548[»]
    1HXGX-ray2.90A1-548[»]
    3LZ9X-ray2.28A1-548[»]
    3M00X-ray2.10A1-548[»]
    3M01X-ray2.60A1-548[»]
    3M02X-ray2.50A1-548[»]
    4DI5X-ray2.30A14-548[»]
    4RNQX-ray2.47A1-548[»]
    5DHIX-ray2.25A13-548[»]
    5DHKX-ray2.43A13-548[»]
    5EASX-ray2.25A1-548[»]
    5EATX-ray2.80A1-548[»]
    5EAUX-ray2.15A1-548[»]
    5IK0X-ray2.20A1-548[»]
    5IK6X-ray2.30A1-548[»]
    5IK9X-ray2.23A1-548[»]
    5IKAX-ray2.45A1-548[»]
    5IKHX-ray2.10A1-548[»]
    ProteinModelPortaliQ40577.
    SMRiQ40577.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    KEGGiag:AAA19216.

    Phylogenomic databases

    KOiK15804.

    Enzyme and pathway databases

    UniPathwayiUPA00213.
    BioCyciMetaCyc:EAS-MONOMER.
    BRENDAi4.2.3.61. 3645.

    Miscellaneous databases

    EvolutionaryTraceiQ40577.

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    1.50.30.10. 1 hit.
    InterProiIPR008949. Isoprenoid_synthase_dom.
    IPR001906. Terpene_synth_N.
    IPR005630. Terpene_synthase_metal-bd.
    IPR008930. Terpenoid_cyclase/PrenylTrfase.
    [Graphical view]
    PfamiPF01397. Terpene_synth. 1 hit.
    PF03936. Terpene_synth_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48239. SSF48239. 1 hit.
    SSF48576. SSF48576. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry namei5EAS_TOBAC
    AccessioniPrimary (citable) accession number: Q40577
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: April 29, 2008
    Last modified: November 30, 2016
    This is version 114 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    PubMed:1438319 indicates the presence of at least two genes coding for the same protein.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.