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Q40546

- KPYG_TOBAC

UniProt

Q40546 - KPYG_TOBAC

Protein

Pyruvate kinase isozyme G, chloroplastic

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei121 – 1211SubstrateBy similarity
    Metal bindingi123 – 1231PotassiumBy similarity
    Metal bindingi125 – 1251PotassiumBy similarity
    Metal bindingi156 – 1561PotassiumBy similarity
    Metal bindingi157 – 1571Potassium; via carbonyl oxygenBy similarity
    Sitei306 – 3061Transition state stabilizerBy similarity
    Metal bindingi308 – 3081MagnesiumSequence Analysis
    Binding sitei331 – 3311Substrate; via amide nitrogenBy similarity
    Metal bindingi332 – 3321MagnesiumBy similarity
    Binding sitei332 – 3321Substrate; via amide nitrogenBy similarity
    Binding sitei364 – 3641SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase isozyme G, chloroplastic (EC:2.7.1.40)
    OrganismiNicotiana tabacum (Common tobacco)
    Taxonomic identifieri4097 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 562Pyruvate kinase isozyme G, chloroplasticPRO_0000016664
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Proteomic databases

    PRIDEiQ40546.

    Expressioni

    Tissue specificityi

    Highest levels in leaves. Also found in stems, roots and flowers.

    Developmental stagei

    Most abundantly expressed during the early globular to early cotyledonary stages of embryo development.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ40546.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q40546-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATMNLPTGL HVAAKPASLD RLSSAKNVGD LFFSDSRHRK RVNTSNQIMA    50
    VQSLEHIHGV NNNVYANYVN FNVPSSGYSL GQESVYLNSP RKTKIVCTIG 100
    PSTSSREMIW KLAEAGMNVA RLNMSHGDHA SHQRTIDLVK EYNAQFEDKV 150
    IAIMLDTKGP EVISGDVPKP ILLKEGQEFN FSIKRGVSTE DTVSVNYDDF 200
    INDVEAGDIL LVDGGMMSLA VKSKTSDIVK CEVIDGGELK SRRHLNVRGK 250
    SATLPSITEK DWDDIKFGVN NQVDFYAVSF VKDAKVVHEL KDYLKSCNAD 300
    IHVIVKIESA DSIPNLHSII SASDGAMVAR GDLGAELPIE EVPLLQEDII 350
    RRCQSMQKPV IVATNMLESM IDHPTPTRAE VSDISIAVRE GADAVMLSGE 400
    TAHGKYPLKA VKVMHIVALR TESSLQKSTS SPSQSAAYKS HMGEMFAFHS 450
    SSMANTLSTP IIVFTRTGSM AIILSHNRPS STVFAFTNNE RVKQRLALYH 500
    GVVPIYMEFS SDAEETFSRA IKLLLSKSLV KDGQYVTLVQ SGAQPIWRRH 550
    STHHIQVRKV QS 562
    Length:562
    Mass (Da):61,868
    Last modified:November 1, 1996 - v1
    Checksum:iD21D50204AA6A66E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28374 mRNA. Translation: CAA82223.1.
    PIRiS44287.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28374 mRNA. Translation: CAA82223.1 .
    PIRi S44287.

    3D structure databases

    ProteinModelPortali Q40546.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q40546.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of plastid pyruvate kinase from castor and tobacco."
      Blakeley S.D., Gottlob-Mchugh S., Wan J., Crews L., Miki B., Ko K., Dennis D.T.
      Plant Mol. Biol. 27:79-89(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Petit Havana SR1.
      Tissue: Seed.

    Entry informationi

    Entry nameiKPYG_TOBAC
    AccessioniPrimary (citable) accession number: Q40546
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3