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Q40546 (KPYG_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase isozyme G, chloroplastic

EC=2.7.1.40
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length562 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Subcellular location

Plastidchloroplast.

Tissue specificity

Highest levels in leaves. Also found in stems, roots and flowers.

Developmental stage

Most abundantly expressed during the early globular to early cotyledonary stages of embryo development.

Sequence similarities

Belongs to the pyruvate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
Pyruvate
   Molecular functionKinase
Transferase
Gene Ontology (GO)
   Biological_processglycolysis

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

potassium ion binding

Inferred from electronic annotation. Source: InterPro

pyruvate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 562Pyruvate kinase isozyme G, chloroplasticPRO_0000016664

Sites

Metal binding1231Potassium By similarity
Metal binding1251Potassium By similarity
Metal binding1561Potassium By similarity
Metal binding1571Potassium; via carbonyl oxygen By similarity
Metal binding3081Magnesium Potential
Metal binding3321Magnesium By similarity
Binding site1211Substrate By similarity
Binding site3311Substrate; via amide nitrogen By similarity
Binding site3321Substrate; via amide nitrogen By similarity
Binding site3641Substrate By similarity
Site3061Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q40546 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: D21D50204AA6A66E

FASTA56261,868
        10         20         30         40         50         60 
MATMNLPTGL HVAAKPASLD RLSSAKNVGD LFFSDSRHRK RVNTSNQIMA VQSLEHIHGV 

        70         80         90        100        110        120 
NNNVYANYVN FNVPSSGYSL GQESVYLNSP RKTKIVCTIG PSTSSREMIW KLAEAGMNVA 

       130        140        150        160        170        180 
RLNMSHGDHA SHQRTIDLVK EYNAQFEDKV IAIMLDTKGP EVISGDVPKP ILLKEGQEFN 

       190        200        210        220        230        240 
FSIKRGVSTE DTVSVNYDDF INDVEAGDIL LVDGGMMSLA VKSKTSDIVK CEVIDGGELK 

       250        260        270        280        290        300 
SRRHLNVRGK SATLPSITEK DWDDIKFGVN NQVDFYAVSF VKDAKVVHEL KDYLKSCNAD 

       310        320        330        340        350        360 
IHVIVKIESA DSIPNLHSII SASDGAMVAR GDLGAELPIE EVPLLQEDII RRCQSMQKPV 

       370        380        390        400        410        420 
IVATNMLESM IDHPTPTRAE VSDISIAVRE GADAVMLSGE TAHGKYPLKA VKVMHIVALR 

       430        440        450        460        470        480 
TESSLQKSTS SPSQSAAYKS HMGEMFAFHS SSMANTLSTP IIVFTRTGSM AIILSHNRPS 

       490        500        510        520        530        540 
STVFAFTNNE RVKQRLALYH GVVPIYMEFS SDAEETFSRA IKLLLSKSLV KDGQYVTLVQ 

       550        560 
SGAQPIWRRH STHHIQVRKV QS 

« Hide

References

[1]"Molecular characterization of plastid pyruvate kinase from castor and tobacco."
Blakeley S.D., Gottlob-Mchugh S., Wan J., Crews L., Miki B., Ko K., Dennis D.T.
Plant Mol. Biol. 27:79-89(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Petit Havana SR1.
Tissue: Seed.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z28374 mRNA. Translation: CAA82223.1.
PIRS44287.

3D structure databases

ProteinModelPortalQ40546.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ40546.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYG_TOBAC
AccessionPrimary (citable) accession number: Q40546
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways