Reviewed, UniProtKB/Swiss-Prot Q40546 (KPYG_TOBAC)

Names and origin

Protein names	Recommended name:     Pyruvate kinase isozyme G, chloroplastic     EC=2.7.1.40
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	562 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Plastid › chloroplast.
Tissue specificity	Highest levels in leaves. Also found in stems, roots and flowers.
Developmental stage	Most abundantly expressed during the early globular to early cotyledonary stages of embryo development.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 562		Pyruvate kinase isozyme G, chloroplastic		PRO_0000016664
Sites
	Active site	306	1	By similarity
	Metal binding	308	1	Magnesium Potential
	Metal binding	329	1	Magnesium Potential
	Metal binding	330	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q40546-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D21D50204AA6A66E	FASTA	562	61,868
	10 20 30 40 50 60 MATMNLPTGL HVAAKPASLD RLSSAKNVGD LFFSDSRHRK RVNTSNQIMA VQSLEHIHGV 70 80 90 100 110 120 NNNVYANYVN FNVPSSGYSL GQESVYLNSP RKTKIVCTIG PSTSSREMIW KLAEAGMNVA 130 140 150 160 170 180 RLNMSHGDHA SHQRTIDLVK EYNAQFEDKV IAIMLDTKGP EVISGDVPKP ILLKEGQEFN 190 200 210 220 230 240 FSIKRGVSTE DTVSVNYDDF INDVEAGDIL LVDGGMMSLA VKSKTSDIVK CEVIDGGELK 250 260 270 280 290 300 SRRHLNVRGK SATLPSITEK DWDDIKFGVN NQVDFYAVSF VKDAKVVHEL KDYLKSCNAD 310 320 330 340 350 360 IHVIVKIESA DSIPNLHSII SASDGAMVAR GDLGAELPIE EVPLLQEDII RRCQSMQKPV 370 380 390 400 410 420 IVATNMLESM IDHPTPTRAE VSDISIAVRE GADAVMLSGE TAHGKYPLKA VKVMHIVALR 430 440 450 460 470 480 TESSLQKSTS SPSQSAAYKS HMGEMFAFHS SSMANTLSTP IIVFTRTGSM AIILSHNRPS 490 500 510 520 530 540 STVFAFTNNE RVKQRLALYH GVVPIYMEFS SDAEETFSRA IKLLLSKSLV KDGQYVTLVQ 550 560 SGAQPIWRRH STHHIQVRKV QS

References

[1]

"Molecular characterization of plastid pyruvate kinase from castor and tobacco." Blakeley S.D., Gottlob-Mchugh S., Wan J., Crews L., Miki B., Ko K., Dennis D.T. Plant Mol. Biol. 27:79-89(1995) [PubMed: 7865798] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Petit Havana SR1. Tissue: Seed.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z28374 mRNA. Translation: CAA82223.1.
PIR	S44287.
3D structure databases
HSSP	HSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.40. 298.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYG_TOBAC

Accession

Primary (citable) accession number: Q40546

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)