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Q40546

- KPYG_TOBAC

UniProt

Q40546 - KPYG_TOBAC

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Protein

Pyruvate kinase isozyme G, chloroplastic

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.By similarity
Potassium.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei121 – 1211SubstrateBy similarity
Metal bindingi123 – 1231PotassiumBy similarity
Metal bindingi125 – 1251PotassiumBy similarity
Metal bindingi156 – 1561PotassiumBy similarity
Metal bindingi157 – 1571Potassium; via carbonyl oxygenBy similarity
Sitei306 – 3061Transition state stabilizerBy similarity
Metal bindingi308 – 3081MagnesiumSequence Analysis
Binding sitei331 – 3311Substrate; via amide nitrogenBy similarity
Metal bindingi332 – 3321MagnesiumBy similarity
Binding sitei332 – 3321Substrate; via amide nitrogenBy similarity
Binding sitei364 – 3641SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase isozyme G, chloroplastic (EC:2.7.1.40)
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 562Pyruvate kinase isozyme G, chloroplasticPRO_0000016664
Transit peptidei1 – ?ChloroplastSequence Analysis

Proteomic databases

PRIDEiQ40546.

Expressioni

Tissue specificityi

Highest levels in leaves. Also found in stems, roots and flowers.

Developmental stagei

Most abundantly expressed during the early globular to early cotyledonary stages of embryo development.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ40546.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q40546 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATMNLPTGL HVAAKPASLD RLSSAKNVGD LFFSDSRHRK RVNTSNQIMA
60 70 80 90 100
VQSLEHIHGV NNNVYANYVN FNVPSSGYSL GQESVYLNSP RKTKIVCTIG
110 120 130 140 150
PSTSSREMIW KLAEAGMNVA RLNMSHGDHA SHQRTIDLVK EYNAQFEDKV
160 170 180 190 200
IAIMLDTKGP EVISGDVPKP ILLKEGQEFN FSIKRGVSTE DTVSVNYDDF
210 220 230 240 250
INDVEAGDIL LVDGGMMSLA VKSKTSDIVK CEVIDGGELK SRRHLNVRGK
260 270 280 290 300
SATLPSITEK DWDDIKFGVN NQVDFYAVSF VKDAKVVHEL KDYLKSCNAD
310 320 330 340 350
IHVIVKIESA DSIPNLHSII SASDGAMVAR GDLGAELPIE EVPLLQEDII
360 370 380 390 400
RRCQSMQKPV IVATNMLESM IDHPTPTRAE VSDISIAVRE GADAVMLSGE
410 420 430 440 450
TAHGKYPLKA VKVMHIVALR TESSLQKSTS SPSQSAAYKS HMGEMFAFHS
460 470 480 490 500
SSMANTLSTP IIVFTRTGSM AIILSHNRPS STVFAFTNNE RVKQRLALYH
510 520 530 540 550
GVVPIYMEFS SDAEETFSRA IKLLLSKSLV KDGQYVTLVQ SGAQPIWRRH
560
STHHIQVRKV QS
Length:562
Mass (Da):61,868
Last modified:November 1, 1996 - v1
Checksum:iD21D50204AA6A66E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z28374 mRNA. Translation: CAA82223.1.
PIRiS44287.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z28374 mRNA. Translation: CAA82223.1 .
PIRi S44287.

3D structure databases

ProteinModelPortali Q40546.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q40546.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of plastid pyruvate kinase from castor and tobacco."
    Blakeley S.D., Gottlob-Mchugh S., Wan J., Crews L., Miki B., Ko K., Dennis D.T.
    Plant Mol. Biol. 27:79-89(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Petit Havana SR1.
    Tissue: Seed.

Entry informationi

Entry nameiKPYG_TOBAC
AccessioniPrimary (citable) accession number: Q40546
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3