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Q40545 (KPYA_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase isozyme A, chloroplastic

EC=2.7.1.40
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length593 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subcellular location

Plastidchloroplast.

Tissue specificity

Highest levels in roots. Also found in stems, leaves and flowers.

Developmental stage

Most abundantly expressed during the early globular to early cotyledonary stages of embryo development.

Sequence similarities

Belongs to the pyruvate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
Pyruvate
   Molecular functionKinase
Transferase
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

potassium ion binding

Inferred from electronic annotation. Source: InterPro

pyruvate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 593Pyruvate kinase isozyme A, chloroplasticPRO_0000016663

Sites

Metal binding1461Potassium By similarity
Metal binding1781Potassium By similarity
Metal binding1791Potassium; via carbonyl oxygen By similarity
Metal binding3431Magnesium Potential
Metal binding3671Magnesium By similarity
Binding site1441Substrate By similarity
Binding site3661Substrate; via amide nitrogen By similarity
Binding site3671Substrate; via amide nitrogen By similarity
Binding site3991Substrate By similarity
Site3411Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q40545 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: FAF049E193C1D484

FASTA59365,228
        10         20         30         40         50         60 
MSQALNFFVS SSSRSPATFT ISRPSVFPST GSLRLLVKKS LRTLVVEASS AAASDLDEPQ 

        70         80         90        100        110        120 
SSPVLVSENG SGGVLSSATQ EYGRNAAPGT DSSSIEVDTV TEAELKENGF RSTRRTKLIC 

       130        140        150        160        170        180 
TIGPATCGFE QLERLAEGGM NVARINMCHG TREWHRMVIE RLRRLNEEKG FAVAIMMDTE 

       190        200        210        220        230        240 
GSEIHMGDLG GASSAKAEDG EIWNFTVRSF DPPLPERTVT VNYDGFAEDV KVGDELLVDG 

       250        260        270        280        290        300 
GMVRFEVIEK IGPDVKCLCT DPGLLLPRAN LTFWRDGKLV RERNAMLPTI SSKDWLDIDF 

       310        320        330        340        350        360 
GIAEGVDFIA VSFVKSAEVI KHLKSYIQAR ARDSDISVIA KIESIDSLKN LEEIIQASDG 

       370        380        390        400        410        420 
AMVARGDLGA QIPLEQVPSE QQKIVQICRQ LNRPVIVASQ LLESMIEYPI PTRAEVADVS 

       430        440        450        460        470        480 
EAVRQRGDAL MLSGESAMGQ FPEKALTVLR SVSLRIERMW REQKRHEVIE LPSIASSFSD 

       490        500        510        520        530        540 
SISEEICNSA AKMANNLEVD ALFVYTKNGH MASLLSRCRP DCPIFAFTTT TSVRRRLNLQ 

       550        560        570        580        590 
WGLMPFRLSF SDDMESNLNK TFSLLKARGM IKSGDLIIAV SDMLQSIQVM NVP 

« Hide

References

[1]"Molecular characterization of plastid pyruvate kinase from castor and tobacco."
Blakeley S.D., Gottlob-Mchugh S., Wan J., Crews L., Miki B., Ko K., Dennis D.T.
Plant Mol. Biol. 27:79-89(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Petit Havana SR1.
Tissue: Seed.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z28373 mRNA. Translation: CAA82222.1.
PIRS51946.

3D structure databases

ProteinModelPortalQ40545.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ40545.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYA_TOBAC
AccessionPrimary (citable) accession number: Q40545
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways