Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q40545

- KPYA_TOBAC

UniProt

Q40545 - KPYA_TOBAC

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Pyruvate kinase isozyme A, chloroplastic

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei144 – 1441SubstrateBy similarity
Metal bindingi146 – 1461PotassiumBy similarity
Metal bindingi178 – 1781PotassiumBy similarity
Metal bindingi179 – 1791Potassium; via carbonyl oxygenBy similarity
Sitei341 – 3411Transition state stabilizerBy similarity
Metal bindingi343 – 3431MagnesiumSequence Analysis
Binding sitei366 – 3661Substrate; via amide nitrogenBy similarity
Metal bindingi367 – 3671MagnesiumBy similarity
Binding sitei367 – 3671Substrate; via amide nitrogenBy similarity
Binding sitei399 – 3991SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase isozyme A, chloroplastic (EC:2.7.1.40)
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 593Pyruvate kinase isozyme A, chloroplasticPRO_0000016663
Transit peptidei1 – ?ChloroplastSequence Analysis

Proteomic databases

PRIDEiQ40545.

Expressioni

Tissue specificityi

Highest levels in roots. Also found in stems, leaves and flowers.

Developmental stagei

Most abundantly expressed during the early globular to early cotyledonary stages of embryo development.

Structurei

3D structure databases

ProteinModelPortaliQ40545.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q40545-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQALNFFVS SSSRSPATFT ISRPSVFPST GSLRLLVKKS LRTLVVEASS
60 70 80 90 100
AAASDLDEPQ SSPVLVSENG SGGVLSSATQ EYGRNAAPGT DSSSIEVDTV
110 120 130 140 150
TEAELKENGF RSTRRTKLIC TIGPATCGFE QLERLAEGGM NVARINMCHG
160 170 180 190 200
TREWHRMVIE RLRRLNEEKG FAVAIMMDTE GSEIHMGDLG GASSAKAEDG
210 220 230 240 250
EIWNFTVRSF DPPLPERTVT VNYDGFAEDV KVGDELLVDG GMVRFEVIEK
260 270 280 290 300
IGPDVKCLCT DPGLLLPRAN LTFWRDGKLV RERNAMLPTI SSKDWLDIDF
310 320 330 340 350
GIAEGVDFIA VSFVKSAEVI KHLKSYIQAR ARDSDISVIA KIESIDSLKN
360 370 380 390 400
LEEIIQASDG AMVARGDLGA QIPLEQVPSE QQKIVQICRQ LNRPVIVASQ
410 420 430 440 450
LLESMIEYPI PTRAEVADVS EAVRQRGDAL MLSGESAMGQ FPEKALTVLR
460 470 480 490 500
SVSLRIERMW REQKRHEVIE LPSIASSFSD SISEEICNSA AKMANNLEVD
510 520 530 540 550
ALFVYTKNGH MASLLSRCRP DCPIFAFTTT TSVRRRLNLQ WGLMPFRLSF
560 570 580 590
SDDMESNLNK TFSLLKARGM IKSGDLIIAV SDMLQSIQVM NVP
Length:593
Mass (Da):65,228
Last modified:November 1, 1996 - v1
Checksum:iFAF049E193C1D484
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28373 mRNA. Translation: CAA82222.1.
PIRiS51946.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z28373 mRNA. Translation: CAA82222.1 .
PIRi S51946.

3D structure databases

ProteinModelPortali Q40545.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q40545.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of plastid pyruvate kinase from castor and tobacco."
    Blakeley S.D., Gottlob-Mchugh S., Wan J., Crews L., Miki B., Ko K., Dennis D.T.
    Plant Mol. Biol. 27:79-89(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Petit Havana SR1.
    Tissue: Seed.

Entry informationi

Entry nameiKPYA_TOBAC
AccessioniPrimary (citable) accession number: Q40545
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3