Reviewed, UniProtKB/Swiss-Prot Q40545 (KPYA_TOBAC)

Names and origin

Protein names	Recommended name:     Pyruvate kinase isozyme A, chloroplastic     EC=2.7.1.40
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subcellular location	Plastid › chloroplast.
Tissue specificity	Highest levels in roots. Also found in stems, leaves and flowers.
Developmental stage	Most abundantly expressed during the early globular to early cotyledonary stages of embryo development.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 593		Pyruvate kinase isozyme A, chloroplastic		PRO_0000016663
Sites
	Active site	341	1	By similarity
	Metal binding	343	1	Magnesium Potential
	Metal binding	364	1	Magnesium Potential
	Metal binding	365	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q40545-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FAF049E193C1D484	FASTA	593	65,228
	10 20 30 40 50 60 MSQALNFFVS SSSRSPATFT ISRPSVFPST GSLRLLVKKS LRTLVVEASS AAASDLDEPQ 70 80 90 100 110 120 SSPVLVSENG SGGVLSSATQ EYGRNAAPGT DSSSIEVDTV TEAELKENGF RSTRRTKLIC 130 140 150 160 170 180 TIGPATCGFE QLERLAEGGM NVARINMCHG TREWHRMVIE RLRRLNEEKG FAVAIMMDTE 190 200 210 220 230 240 GSEIHMGDLG GASSAKAEDG EIWNFTVRSF DPPLPERTVT VNYDGFAEDV KVGDELLVDG 250 260 270 280 290 300 GMVRFEVIEK IGPDVKCLCT DPGLLLPRAN LTFWRDGKLV RERNAMLPTI SSKDWLDIDF 310 320 330 340 350 360 GIAEGVDFIA VSFVKSAEVI KHLKSYIQAR ARDSDISVIA KIESIDSLKN LEEIIQASDG 370 380 390 400 410 420 AMVARGDLGA QIPLEQVPSE QQKIVQICRQ LNRPVIVASQ LLESMIEYPI PTRAEVADVS 430 440 450 460 470 480 EAVRQRGDAL MLSGESAMGQ FPEKALTVLR SVSLRIERMW REQKRHEVIE LPSIASSFSD 490 500 510 520 530 540 SISEEICNSA AKMANNLEVD ALFVYTKNGH MASLLSRCRP DCPIFAFTTT TSVRRRLNLQ 550 560 570 580 590 WGLMPFRLSF SDDMESNLNK TFSLLKARGM IKSGDLIIAV SDMLQSIQVM NVP

References

[1]

"Molecular characterization of plastid pyruvate kinase from castor and tobacco." Blakeley S.D., Gottlob-Mchugh S., Wan J., Crews L., Miki B., Ko K., Dennis D.T. Plant Mol. Biol. 27:79-89(1995) [PubMed: 7865798] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Petit Havana SR1. Tissue: Seed.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z28373 mRNA. Translation: CAA82222.1.
PIR	S51946.
3D structure databases
HSSP	HSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.40. 298.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYA_TOBAC

Accession

Primary (citable) accession number: Q40545

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)