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Q40545

- KPYA_TOBAC

UniProt

Q40545 - KPYA_TOBAC

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Protein
Pyruvate kinase isozyme A, chloroplastic
Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium By similarity.
Potassium By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei144 – 1441Substrate By similarity
Metal bindingi146 – 1461Potassium By similarity
Metal bindingi178 – 1781Potassium By similarity
Metal bindingi179 – 1791Potassium; via carbonyl oxygen By similarity
Sitei341 – 3411Transition state stabilizer By similarity
Metal bindingi343 – 3431Magnesium Reviewed prediction
Binding sitei366 – 3661Substrate; via amide nitrogen By similarity
Metal bindingi367 – 3671Magnesium By similarity
Binding sitei367 – 3671Substrate; via amide nitrogen By similarity
Binding sitei399 – 3991Substrate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase isozyme A, chloroplastic (EC:2.7.1.40)
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 593Pyruvate kinase isozyme A, chloroplasticPRO_0000016663
Transit peptidei1 – ?Chloroplast Reviewed prediction

Proteomic databases

PRIDEiQ40545.

Expressioni

Tissue specificityi

Highest levels in roots. Also found in stems, leaves and flowers.

Developmental stagei

Most abundantly expressed during the early globular to early cotyledonary stages of embryo development.

Structurei

3D structure databases

ProteinModelPortaliQ40545.

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q40545-1 [UniParc]FASTAAdd to Basket

« Hide

MSQALNFFVS SSSRSPATFT ISRPSVFPST GSLRLLVKKS LRTLVVEASS    50
AAASDLDEPQ SSPVLVSENG SGGVLSSATQ EYGRNAAPGT DSSSIEVDTV 100
TEAELKENGF RSTRRTKLIC TIGPATCGFE QLERLAEGGM NVARINMCHG 150
TREWHRMVIE RLRRLNEEKG FAVAIMMDTE GSEIHMGDLG GASSAKAEDG 200
EIWNFTVRSF DPPLPERTVT VNYDGFAEDV KVGDELLVDG GMVRFEVIEK 250
IGPDVKCLCT DPGLLLPRAN LTFWRDGKLV RERNAMLPTI SSKDWLDIDF 300
GIAEGVDFIA VSFVKSAEVI KHLKSYIQAR ARDSDISVIA KIESIDSLKN 350
LEEIIQASDG AMVARGDLGA QIPLEQVPSE QQKIVQICRQ LNRPVIVASQ 400
LLESMIEYPI PTRAEVADVS EAVRQRGDAL MLSGESAMGQ FPEKALTVLR 450
SVSLRIERMW REQKRHEVIE LPSIASSFSD SISEEICNSA AKMANNLEVD 500
ALFVYTKNGH MASLLSRCRP DCPIFAFTTT TSVRRRLNLQ WGLMPFRLSF 550
SDDMESNLNK TFSLLKARGM IKSGDLIIAV SDMLQSIQVM NVP 593
Length:593
Mass (Da):65,228
Last modified:November 1, 1996 - v1
Checksum:iFAF049E193C1D484
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z28373 mRNA. Translation: CAA82222.1.
PIRiS51946.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z28373 mRNA. Translation: CAA82222.1 .
PIRi S51946.

3D structure databases

ProteinModelPortali Q40545.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q40545.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization of plastid pyruvate kinase from castor and tobacco."
    Blakeley S.D., Gottlob-Mchugh S., Wan J., Crews L., Miki B., Ko K., Dennis D.T.
    Plant Mol. Biol. 27:79-89(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Petit Havana SR1.
    Tissue: Seed.

Entry informationi

Entry nameiKPYA_TOBAC
AccessioniPrimary (citable) accession number: Q40545
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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