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Q40545

- KPYA_TOBAC

UniProt

Q40545 - KPYA_TOBAC

Protein

Pyruvate kinase isozyme A, chloroplastic

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei144 – 1441SubstrateBy similarity
    Metal bindingi146 – 1461PotassiumBy similarity
    Metal bindingi178 – 1781PotassiumBy similarity
    Metal bindingi179 – 1791Potassium; via carbonyl oxygenBy similarity
    Sitei341 – 3411Transition state stabilizerBy similarity
    Metal bindingi343 – 3431MagnesiumSequence Analysis
    Binding sitei366 – 3661Substrate; via amide nitrogenBy similarity
    Metal bindingi367 – 3671MagnesiumBy similarity
    Binding sitei367 – 3671Substrate; via amide nitrogenBy similarity
    Binding sitei399 – 3991SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase isozyme A, chloroplastic (EC:2.7.1.40)
    OrganismiNicotiana tabacum (Common tobacco)
    Taxonomic identifieri4097 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 593Pyruvate kinase isozyme A, chloroplasticPRO_0000016663
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Proteomic databases

    PRIDEiQ40545.

    Expressioni

    Tissue specificityi

    Highest levels in roots. Also found in stems, leaves and flowers.

    Developmental stagei

    Most abundantly expressed during the early globular to early cotyledonary stages of embryo development.

    Structurei

    3D structure databases

    ProteinModelPortaliQ40545.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q40545-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQALNFFVS SSSRSPATFT ISRPSVFPST GSLRLLVKKS LRTLVVEASS    50
    AAASDLDEPQ SSPVLVSENG SGGVLSSATQ EYGRNAAPGT DSSSIEVDTV 100
    TEAELKENGF RSTRRTKLIC TIGPATCGFE QLERLAEGGM NVARINMCHG 150
    TREWHRMVIE RLRRLNEEKG FAVAIMMDTE GSEIHMGDLG GASSAKAEDG 200
    EIWNFTVRSF DPPLPERTVT VNYDGFAEDV KVGDELLVDG GMVRFEVIEK 250
    IGPDVKCLCT DPGLLLPRAN LTFWRDGKLV RERNAMLPTI SSKDWLDIDF 300
    GIAEGVDFIA VSFVKSAEVI KHLKSYIQAR ARDSDISVIA KIESIDSLKN 350
    LEEIIQASDG AMVARGDLGA QIPLEQVPSE QQKIVQICRQ LNRPVIVASQ 400
    LLESMIEYPI PTRAEVADVS EAVRQRGDAL MLSGESAMGQ FPEKALTVLR 450
    SVSLRIERMW REQKRHEVIE LPSIASSFSD SISEEICNSA AKMANNLEVD 500
    ALFVYTKNGH MASLLSRCRP DCPIFAFTTT TSVRRRLNLQ WGLMPFRLSF 550
    SDDMESNLNK TFSLLKARGM IKSGDLIIAV SDMLQSIQVM NVP 593
    Length:593
    Mass (Da):65,228
    Last modified:November 1, 1996 - v1
    Checksum:iFAF049E193C1D484
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28373 mRNA. Translation: CAA82222.1.
    PIRiS51946.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z28373 mRNA. Translation: CAA82222.1 .
    PIRi S51946.

    3D structure databases

    ProteinModelPortali Q40545.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q40545.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of plastid pyruvate kinase from castor and tobacco."
      Blakeley S.D., Gottlob-Mchugh S., Wan J., Crews L., Miki B., Ko K., Dennis D.T.
      Plant Mol. Biol. 27:79-89(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Petit Havana SR1.
      Tissue: Seed.

    Entry informationi

    Entry nameiKPYA_TOBAC
    AccessioniPrimary (citable) accession number: Q40545
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3