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Q40532

- NTF4_TOBAC

UniProt

Q40532 - NTF4_TOBAC

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Protein
Mitogen-activated protein kinase homolog NTF4
Gene
NTF4
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891ATP By similarity
Active sitei186 – 1861Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 749ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24. 3645.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase homolog NTF4 (EC:2.7.11.24)
    Alternative name(s):
    P45
    Gene namesi
    Name:NTF4
    OrganismiNicotiana tabacum (Common tobacco)
    Taxonomic identifieri4097 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 393393Mitogen-activated protein kinase homolog NTF4
    PRO_0000186322Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei218 – 2181Phosphothreonine By similarity
    Modified residuei220 – 2201Phosphotyrosine By similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-218 and Tyr-220, which activates the enzyme By similarity. Very low autophosphorylation, although dramatically increased when Mn2+ is added to the reaction instead of Mg2+.

    Keywords - PTMi

    Phosphoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliQ40532.
    SMRiQ40532. Positions 49-392.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini60 – 345286Protein kinase
    Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi218 – 2203TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q40532-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDGPAHQTDT VMSDAAGQQP APPSQPVAGI DNIPATLSHG GRFIQYNIFG    50
    NIFEVTAKYK PPIMPIGKGA YGIVCSALNS ETNEHVAIKK IANAFDNKID 100
    AKRTLREIKL LRHMDHENIV AIRDIIPPPQ REAFNDVYIA YELMDTDLHQ 150
    IIRSNQGLSE EHCQYFLYQI LRGLKYIHSA NVLHRDLKPS NLLLNANCDL 200
    KICDFGLARV TSETDFMTEY VVTRWYRAPE LLLNSSDYTA AIDVWSVGCI 250
    FMELMDRKPL FPGRDHVHQL RLLMELIGTP SEAEMEFLNE NAKRYIRQLP 300
    LYRRQSFVEK FPHVNPAAID LVEKMLTFDP RRRITVEDAL AHPYLTSLHD 350
    ISDEPVCMTP FNFDFEQHAL TEEQMKELIY REGLAFNPEY QHM 393
    Length:393
    Mass (Da):45,120
    Last modified:November 1, 1996 - v1
    Checksum:i76EC1F3B1F174AB9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83880 mRNA. Translation: CAA58761.1.
    PIRiS68190. S51321.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X83880 mRNA. Translation: CAA58761.1 .
    PIRi S68190. S51321.

    3D structure databases

    ProteinModelPortali Q40532.
    SMRi Q40532. Positions 49-392.
    ModBasei Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 2.7.11.24. 3645.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, functional expression in Escherichia coli, and characterization of multiple mitogen-activated-protein kinases from tobacco."
      Wilson C., Anglmayer R., Vicente O., Heberle-Bors E.
      Eur. J. Biochem. 233:249-257(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Petit Havana SR1.

    Entry informationi

    Entry nameiNTF4_TOBAC
    AccessioniPrimary (citable) accession number: Q40532
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: April 16, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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