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Q40532

- NTF4_TOBAC

UniProt

Q40532 - NTF4_TOBAC

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Protein

Mitogen-activated protein kinase homolog NTF4

Gene

NTF4

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei89 – 891ATPPROSITE-ProRule annotation
Active sitei186 – 1861Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi66 – 749ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 3645.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase homolog NTF4 (EC:2.7.11.24)
Alternative name(s):
P45
Gene namesi
Name:NTF4
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 393393Mitogen-activated protein kinase homolog NTF4PRO_0000186322Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei218 – 2181PhosphothreonineBy similarity
Modified residuei220 – 2201PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-218 and Tyr-220, which activates the enzyme (By similarity). Very low autophosphorylation, although dramatically increased when Mn2+ is added to the reaction instead of Mg2+.By similarity

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ40532.
SMRiQ40532. Positions 49-392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini60 – 345286Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi218 – 2203TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q40532-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDGPAHQTDT VMSDAAGQQP APPSQPVAGI DNIPATLSHG GRFIQYNIFG
60 70 80 90 100
NIFEVTAKYK PPIMPIGKGA YGIVCSALNS ETNEHVAIKK IANAFDNKID
110 120 130 140 150
AKRTLREIKL LRHMDHENIV AIRDIIPPPQ REAFNDVYIA YELMDTDLHQ
160 170 180 190 200
IIRSNQGLSE EHCQYFLYQI LRGLKYIHSA NVLHRDLKPS NLLLNANCDL
210 220 230 240 250
KICDFGLARV TSETDFMTEY VVTRWYRAPE LLLNSSDYTA AIDVWSVGCI
260 270 280 290 300
FMELMDRKPL FPGRDHVHQL RLLMELIGTP SEAEMEFLNE NAKRYIRQLP
310 320 330 340 350
LYRRQSFVEK FPHVNPAAID LVEKMLTFDP RRRITVEDAL AHPYLTSLHD
360 370 380 390
ISDEPVCMTP FNFDFEQHAL TEEQMKELIY REGLAFNPEY QHM
Length:393
Mass (Da):45,120
Last modified:November 1, 1996 - v1
Checksum:i76EC1F3B1F174AB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83880 mRNA. Translation: CAA58761.1.
PIRiS68190. S51321.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83880 mRNA. Translation: CAA58761.1 .
PIRi S68190. S51321.

3D structure databases

ProteinModelPortali Q40532.
SMRi Q40532. Positions 49-392.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 2.7.11.24. 3645.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning, functional expression in Escherichia coli, and characterization of multiple mitogen-activated-protein kinases from tobacco."
    Wilson C., Anglmayer R., Vicente O., Heberle-Bors E.
    Eur. J. Biochem. 233:249-257(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Petit Havana SR1.

Entry informationi

Entry nameiNTF4_TOBAC
AccessioniPrimary (citable) accession number: Q40532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3