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Q40532 (NTF4_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase homolog NTF4

EC=2.7.11.24
Alternative name(s):
P45
Gene names
Name:NTF4
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length393 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by tyrosine and threonine phosphorylation By similarity.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-218 and Tyr-220, which activates the enzyme By similarity. Very low autophosphorylation, although dramatically increased when Mn2+ is added to the reaction instead of Mg2+.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 393393Mitogen-activated protein kinase homolog NTF4
PRO_0000186322

Regions

Domain60 – 345286Protein kinase
Nucleotide binding66 – 749ATP By similarity
Motif218 – 2203TXY

Sites

Active site1861Proton acceptor By similarity
Binding site891ATP By similarity

Amino acid modifications

Modified residue2181Phosphothreonine By similarity
Modified residue2201Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q40532 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 76EC1F3B1F174AB9

FASTA39345,120
        10         20         30         40         50         60 
MDGPAHQTDT VMSDAAGQQP APPSQPVAGI DNIPATLSHG GRFIQYNIFG NIFEVTAKYK 

        70         80         90        100        110        120 
PPIMPIGKGA YGIVCSALNS ETNEHVAIKK IANAFDNKID AKRTLREIKL LRHMDHENIV 

       130        140        150        160        170        180 
AIRDIIPPPQ REAFNDVYIA YELMDTDLHQ IIRSNQGLSE EHCQYFLYQI LRGLKYIHSA 

       190        200        210        220        230        240 
NVLHRDLKPS NLLLNANCDL KICDFGLARV TSETDFMTEY VVTRWYRAPE LLLNSSDYTA 

       250        260        270        280        290        300 
AIDVWSVGCI FMELMDRKPL FPGRDHVHQL RLLMELIGTP SEAEMEFLNE NAKRYIRQLP 

       310        320        330        340        350        360 
LYRRQSFVEK FPHVNPAAID LVEKMLTFDP RRRITVEDAL AHPYLTSLHD ISDEPVCMTP 

       370        380        390 
FNFDFEQHAL TEEQMKELIY REGLAFNPEY QHM 

« Hide

References

[1]"Molecular cloning, functional expression in Escherichia coli, and characterization of multiple mitogen-activated-protein kinases from tobacco."
Wilson C., Anglmayer R., Vicente O., Heberle-Bors E.
Eur. J. Biochem. 233:249-257(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Petit Havana SR1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83880 mRNA. Translation: CAA58761.1.
PIRS51321. S68190.

3D structure databases

ProteinModelPortalQ40532.
SMRQ40532. Positions 49-392.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.7.11.24. 3645.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNTF4_TOBAC
AccessionPrimary (citable) accession number: Q40532
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families