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Protein

Mitogen-activated protein kinase homolog NTF4

Gene

NTF4

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89ATPPROSITE-ProRule annotation1
Active sitei186Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi66 – 74ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 3645.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase homolog NTF4 (EC:2.7.11.24)
Alternative name(s):
P45
Gene namesi
Name:NTF4
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863221 – 393Mitogen-activated protein kinase homolog NTF4Add BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei218PhosphothreonineBy similarity1
Modified residuei220PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-218 and Tyr-220, which activates the enzyme (By similarity). Very low autophosphorylation, although dramatically increased when Mn2+ is added to the reaction instead of Mg2+.By similarity

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ40532.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 345Protein kinasePROSITE-ProRule annotationAdd BLAST286

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi218 – 220TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q40532-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGPAHQTDT VMSDAAGQQP APPSQPVAGI DNIPATLSHG GRFIQYNIFG
60 70 80 90 100
NIFEVTAKYK PPIMPIGKGA YGIVCSALNS ETNEHVAIKK IANAFDNKID
110 120 130 140 150
AKRTLREIKL LRHMDHENIV AIRDIIPPPQ REAFNDVYIA YELMDTDLHQ
160 170 180 190 200
IIRSNQGLSE EHCQYFLYQI LRGLKYIHSA NVLHRDLKPS NLLLNANCDL
210 220 230 240 250
KICDFGLARV TSETDFMTEY VVTRWYRAPE LLLNSSDYTA AIDVWSVGCI
260 270 280 290 300
FMELMDRKPL FPGRDHVHQL RLLMELIGTP SEAEMEFLNE NAKRYIRQLP
310 320 330 340 350
LYRRQSFVEK FPHVNPAAID LVEKMLTFDP RRRITVEDAL AHPYLTSLHD
360 370 380 390
ISDEPVCMTP FNFDFEQHAL TEEQMKELIY REGLAFNPEY QHM
Length:393
Mass (Da):45,120
Last modified:November 1, 1996 - v1
Checksum:i76EC1F3B1F174AB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83880 mRNA. Translation: CAA58761.1.
PIRiS68190. S51321.
UniGeneiNta.4211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X83880 mRNA. Translation: CAA58761.1.
PIRiS68190. S51321.
UniGeneiNta.4211.

3D structure databases

ProteinModelPortaliQ40532.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi2.7.11.24. 3645.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNTF4_TOBAC
AccessioniPrimary (citable) accession number: Q40532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.