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Q40531

- NTF6_TOBAC

UniProt

Q40531 - NTF6_TOBAC

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Protein
Mitogen-activated protein kinase homolog NTF6
Gene
NTF6
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei67 – 671ATP By similarity
Active sitei164 – 1641Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi44 – 529ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 3645.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase homolog NTF6 (EC:2.7.11.24)
Alternative name(s):
P43
Gene namesi
Name:NTF6
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 371371Mitogen-activated protein kinase homolog NTF6
PRO_0000186323Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei196 – 1961Phosphothreonine By similarity
Modified residuei198 – 1981Phosphotyrosine By similarity

Post-translational modificationi

Dually phosphorylated on Thr-196 and Tyr-198, which activates the enzyme By similarity. Very low autophosphorylation, although dramatically increased when Mn2+ is added to the reaction instead of Mg2+.

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ40531.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini38 – 324287Protein kinase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi196 – 1983TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q40531-1 [UniParc]FASTAAdd to Basket

« Hide

MENETNEKLE IKGIPTHEGK YVEYNVLGNF FEVTSKYIPP IQPVGRGAYG    50
MVCCATNSET KEEVAIKKIG NAFENRIDAK RTLREIKLLS HMDHENIIKI 100
KDIVRPPDRE EFNDVYIVYE LMDTDLHQII RSSQALTDDH CQYFLYQLLR 150
GLKYVHSANV LHRDLKPSNL LLNANCDLKI CDFGLARTTS EADFMTEYVV 200
TRWYRAPELL LNCTEYTAAI DIWSVGCILM ELIKREPLFP GRDYAQQLGL 250
IIALLGSPED SDLGFLRSDN ARKYVKHLPR VPRHPFSQKF PDVSPLALDL 300
AERMLVFDPA KRITVEDALN HPFLISLHEI NEEPVCDSPF NFDFEQASLS 350
EDDIKELIWN EALKFDPNTM K 371
Length:371
Mass (Da):42,742
Last modified:November 1, 1996 - v1
Checksum:i4D97C41AC203C272
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83879 mRNA. Translation: CAA58760.1.
PIRiS68189. S51320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83879 mRNA. Translation: CAA58760.1 .
PIRi S68189. S51320.

3D structure databases

ProteinModelPortali Q40531.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 2.7.11.24. 3645.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning, functional expression in Escherichia coli, and characterization of multiple mitogen-activated-protein kinases from tobacco."
    Wilson C., Anglmayer R., Vicente O., Heberle-Bors E.
    Eur. J. Biochem. 233:249-257(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Petit Havana SR1.

Entry informationi

Entry nameiNTF6_TOBAC
AccessioniPrimary (citable) accession number: Q40531
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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