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Q40531 (NTF6_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase homolog NTF6

EC=2.7.11.24
Alternative name(s):
P43
Gene names
Name:NTF6
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length371 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by tyrosine and threonine phosphorylation By similarity.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-196 and Tyr-198, which activates the enzyme By similarity. Very low autophosphorylation, although dramatically increased when Mn2+ is added to the reaction instead of Mg2+.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 371371Mitogen-activated protein kinase homolog NTF6
PRO_0000186323

Regions

Domain38 – 324287Protein kinase
Nucleotide binding44 – 529ATP By similarity
Motif196 – 1983TXY

Sites

Active site1641Proton acceptor By similarity
Binding site671ATP By similarity

Amino acid modifications

Modified residue1961Phosphothreonine By similarity
Modified residue1981Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q40531 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 4D97C41AC203C272

FASTA37142,742
        10         20         30         40         50         60 
MENETNEKLE IKGIPTHEGK YVEYNVLGNF FEVTSKYIPP IQPVGRGAYG MVCCATNSET 

        70         80         90        100        110        120 
KEEVAIKKIG NAFENRIDAK RTLREIKLLS HMDHENIIKI KDIVRPPDRE EFNDVYIVYE 

       130        140        150        160        170        180 
LMDTDLHQII RSSQALTDDH CQYFLYQLLR GLKYVHSANV LHRDLKPSNL LLNANCDLKI 

       190        200        210        220        230        240 
CDFGLARTTS EADFMTEYVV TRWYRAPELL LNCTEYTAAI DIWSVGCILM ELIKREPLFP 

       250        260        270        280        290        300 
GRDYAQQLGL IIALLGSPED SDLGFLRSDN ARKYVKHLPR VPRHPFSQKF PDVSPLALDL 

       310        320        330        340        350        360 
AERMLVFDPA KRITVEDALN HPFLISLHEI NEEPVCDSPF NFDFEQASLS EDDIKELIWN 

       370 
EALKFDPNTM K 

« Hide

References

[1]"Molecular cloning, functional expression in Escherichia coli, and characterization of multiple mitogen-activated-protein kinases from tobacco."
Wilson C., Anglmayer R., Vicente O., Heberle-Bors E.
Eur. J. Biochem. 233:249-257(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Petit Havana SR1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X83879 mRNA. Translation: CAA58760.1.
PIRS51320. S68189.

3D structure databases

ProteinModelPortalQ40531.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.7.11.24. 3645.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNTF6_TOBAC
AccessionPrimary (citable) accession number: Q40531
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families