Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q40517

- NTF3_TOBAC

UniProt

Q40517 - NTF3_TOBAC

Protein

Mitogen-activated protein kinase homolog NTF3

Gene

NTF3

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by tyrosine and threonine phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei61 – 611ATPCurated
    Active sitei158 – 1581Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi38 – 469ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24. 3645.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase homolog NTF3 (EC:2.7.11.24)
    Alternative name(s):
    P43
    Gene namesi
    Name:NTF3
    OrganismiNicotiana tabacum (Common tobacco)
    Taxonomic identifieri4097 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi61 – 611K → R: Inactivation. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 372372Mitogen-activated protein kinase homolog NTF3PRO_0000186321Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei191 – 1911PhosphothreonineBy similarity
    Modified residuei193 – 1931PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity. Very low autophosphorylation, although dramatically increased when Mn2+ is added to the reaction instead of Mg2+.By similarity

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Developmental stagei

    Detected during gametophytic pollen development and constitutively expressed in embryogenic pollen.

    Structurei

    3D structure databases

    ProteinModelPortaliQ40517.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini32 – 319288Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi191 – 1933TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q40517-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATPVEPPNG IRTPGKHYYS MWQSLFEIDT KYVPIKPIGR GAYGIVCSSV    50
    NRETNEKVAI KKINNAFENR IDALRTLREL KLLRHLRHEN VIALKDVMMP 100
    IHRRSFKDVY LVYELMDTDL HQIIKSSQTL SNDHCQYFLF QLLRGLKYLH 150
    SANILHRDLK PGNLLINANC DLKICDFGLA RTSSGKDQFM TEYVVTRWYR 200
    APELLLCCDN YGTSIDVWSV GCIFAELLGR KPVFPGTECL NQLKLIINIL 250
    GSQREEDIEF IDNPKARKYI KSLPYSPGTP FSRLYPHAHP LAIDLLQRML 300
    VFDPSKRISV IEALQHPYMS PLYDPNTDPP AQVPINLDID EDLGEETIRE 350
    MMWSEILEYH PEAATAAMEV VL 372
    Length:372
    Mass (Da):42,776
    Last modified:November 1, 1996 - v1
    Checksum:i3277355C89FF3EBA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69971 mRNA. Translation: CAA49592.1.
    PIRiS39559.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69971 mRNA. Translation: CAA49592.1 .
    PIRi S39559.

    3D structure databases

    ProteinModelPortali Q40517.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 2.7.11.24. 3645.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a tobacco cDNA clone encoding a putative MAP kinase."
      Wilson C., Eller N., Gartner A., Vicente O., Heberle-Bors E.
      Plant Mol. Biol. 23:543-551(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Petit Havana SR1.
    2. "Molecular cloning, functional expression in Escherichia coli, and characterization of multiple mitogen-activated-protein kinases from tobacco."
      Wilson C., Anglmayer R., Vicente O., Heberle-Bors E.
      Eur. J. Biochem. 233:249-257(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
      Strain: cv. Petit Havana SR1.

    Entry informationi

    Entry nameiNTF3_TOBAC
    AccessioniPrimary (citable) accession number: Q40517
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3