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Protein

Mitogen-activated protein kinase homolog NTF3

Gene

NTF3

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61ATPCurated1
Active sitei158Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 46ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 3645.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase homolog NTF3 (EC:2.7.11.24)
Alternative name(s):
P43
Gene namesi
Name:NTF3
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana
Proteomesi
  • UP000084051 Componenti: Genome assembly

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61K → R: Inactivation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863211 – 372Mitogen-activated protein kinase homolog NTF3Add BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei191PhosphothreonineBy similarity1
Modified residuei193PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme (By similarity). Very low autophosphorylation, although dramatically increased when Mn2+ is added to the reaction instead of Mg2+.By similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Tissue specificityi

Ubiquitous.

Developmental stagei

Detected during gametophytic pollen development and constitutively expressed in embryogenic pollen.

Structurei

3D structure databases

ProteinModelPortaliQ40517.
SMRiQ40517.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 319Protein kinasePROSITE-ProRule annotationAdd BLAST288

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi191 – 193TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Q40517-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPVEPPNG IRTPGKHYYS MWQSLFEIDT KYVPIKPIGR GAYGIVCSSV
60 70 80 90 100
NRETNEKVAI KKINNAFENR IDALRTLREL KLLRHLRHEN VIALKDVMMP
110 120 130 140 150
IHRRSFKDVY LVYELMDTDL HQIIKSSQTL SNDHCQYFLF QLLRGLKYLH
160 170 180 190 200
SANILHRDLK PGNLLINANC DLKICDFGLA RTSSGKDQFM TEYVVTRWYR
210 220 230 240 250
APELLLCCDN YGTSIDVWSV GCIFAELLGR KPVFPGTECL NQLKLIINIL
260 270 280 290 300
GSQREEDIEF IDNPKARKYI KSLPYSPGTP FSRLYPHAHP LAIDLLQRML
310 320 330 340 350
VFDPSKRISV IEALQHPYMS PLYDPNTDPP AQVPINLDID EDLGEETIRE
360 370
MMWSEILEYH PEAATAAMEV VL
Length:372
Mass (Da):42,776
Last modified:November 1, 1996 - v1
Checksum:i3277355C89FF3EBA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69971 mRNA. Translation: CAA49592.1.
PIRiS39559.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiNTF3_TOBAC
AccessioniPrimary (citable) accession number: Q40517
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 10, 2017
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families