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Q40517 (NTF3_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase homolog NTF3

EC=2.7.11.24
Alternative name(s):
P43
Gene names
Name:NTF3
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by tyrosine and threonine phosphorylation By similarity.

Tissue specificity

Ubiquitous.

Developmental stage

Detected during gametophytic pollen development and constitutively expressed in embryogenic pollen.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-191 and Tyr-193, which activates the enzyme By similarity. Very low autophosphorylation, although dramatically increased when Mn2+ is added to the reaction instead of Mg2+.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Mitogen-activated protein kinase homolog NTF3
PRO_0000186321

Regions

Domain32 – 319288Protein kinase
Nucleotide binding38 – 469ATP By similarity
Motif191 – 1933TXY

Sites

Active site1581Proton acceptor By similarity
Binding site611ATP Probable

Amino acid modifications

Modified residue1911Phosphothreonine By similarity
Modified residue1931Phosphotyrosine By similarity

Experimental info

Mutagenesis611K → R: Inactivation.

Sequences

Sequence LengthMass (Da)Tools
Q40517 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 3277355C89FF3EBA

FASTA37242,776
        10         20         30         40         50         60 
MATPVEPPNG IRTPGKHYYS MWQSLFEIDT KYVPIKPIGR GAYGIVCSSV NRETNEKVAI 

        70         80         90        100        110        120 
KKINNAFENR IDALRTLREL KLLRHLRHEN VIALKDVMMP IHRRSFKDVY LVYELMDTDL 

       130        140        150        160        170        180 
HQIIKSSQTL SNDHCQYFLF QLLRGLKYLH SANILHRDLK PGNLLINANC DLKICDFGLA 

       190        200        210        220        230        240 
RTSSGKDQFM TEYVVTRWYR APELLLCCDN YGTSIDVWSV GCIFAELLGR KPVFPGTECL 

       250        260        270        280        290        300 
NQLKLIINIL GSQREEDIEF IDNPKARKYI KSLPYSPGTP FSRLYPHAHP LAIDLLQRML 

       310        320        330        340        350        360 
VFDPSKRISV IEALQHPYMS PLYDPNTDPP AQVPINLDID EDLGEETIRE MMWSEILEYH 

       370 
PEAATAAMEV VL 

« Hide

References

[1]"Isolation and characterization of a tobacco cDNA clone encoding a putative MAP kinase."
Wilson C., Eller N., Gartner A., Vicente O., Heberle-Bors E.
Plant Mol. Biol. 23:543-551(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Petit Havana SR1.
[2]"Molecular cloning, functional expression in Escherichia coli, and characterization of multiple mitogen-activated-protein kinases from tobacco."
Wilson C., Anglmayer R., Vicente O., Heberle-Bors E.
Eur. J. Biochem. 233:249-257(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
Strain: cv. Petit Havana SR1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X69971 mRNA. Translation: CAA49592.1.
PIRS39559.

3D structure databases

ProteinModelPortalQ40517.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.7.11.24. 3645.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNTF3_TOBAC
AccessionPrimary (citable) accession number: Q40517
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families