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Protein

Ethylene-responsive transcription factor 4

Gene

ERF4

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways mediated by ethylene, that seems to depend on a protein kinase/phosphatase cascade, and to be influenced by methyl-jasmonate. May regulate other AtERFs (By similarity).By similarity5 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi26 – 8358AP2/ERFPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Ethylene signaling pathway, Plant defense, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene-responsive transcription factor 4
Alternative name(s):
Ethylene-responsive element-binding factor 3
Short name:
EREBP-3
Ethylene-responsive element-binding factor 4 homolog
NtERF3
Gene namesi
Name:ERF4
Synonyms:ERF-3, ERF3
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 225225Ethylene-responsive transcription factor 4PRO_0000112556Add
BLAST

Post-translational modificationi

Probably negatively regulated by UBC2, that targets it to the ubiquitin proteasome pathway after ubiquitination and leads it to proteolysis.Curated

Keywords - PTMi

Ubl conjugation

Expressioni

Tissue specificityi

Expressed in roots, in cultured cells and at low levels in buds and leaves. Highly expressed in cell culture, especially during the exponential phase.2 Publications

Inductioni

Strongly induced by ethephon (ethylene-releasing compound) in buds and to lower extent in leaves. Strongly induced by cycloheximide and mechanical stimuli. Wounding leads to a both local and systemical transient expression, independently of ethylene, and through a de-novo-protein-synthesis-independent regulation. Not influenced by methyl-jasmonate. Induction by purified xylanase from Trichoderma viride (TvX) and another elicitor from Phytophthora infestans (PiE), that appears to be mediated by a protein kinase cascade, and to be negatively regulated by protein phosphatases.4 Publications

Interactioni

Subunit structurei

Interacts with UBC2.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ40477.
SMRiQ40477. Positions 26-83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni92 – 196105Interaction with UBC2Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi215 – 2217EAR-like (transcriptional repression)Sequence analysis

Domaini

The AP2/ERF domain binds specifically to the 5'-GCCGCC-3' motif. The affinity of this binding is higher if the seventh amino-acid of this domain is basic (By similarity).By similarity
Contains a slightly degenerated ERF-associated amphiphilic repression (EAR) motif, which may be involved in the activity of transcriptional repression.By similarity

Sequence similaritiesi

Contains 1 AP2/ERF DNA-binding domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q40477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVKNKVSNG NLKGGNVKTD GVKEVHYRGV RKRPWGRYAA EIRDPGKKSR
60 70 80 90 100
VWLGTFDTAE EAAKAYDTAA REFRGPKAKT NFPSPTENQS PSHSSTVESS
110 120 130 140 150
SGENGVHAPP HAPLELDLTR RLGSVAADGG DNCRRSGEVG YPIFHQQPTV
160 170 180 190 200
AVLPNGQPVL LFDSLWRAGV VNRPQPYHVT PMGFNGVNAG VGPTVSDSSS
210 220
AVEENQYDGK RGIDLDLNLA PPMEF
Length:225
Mass (Da):24,262
Last modified:November 1, 1996 - v1
Checksum:iBFF148E5B0B4EFAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38124 mRNA. Translation: BAA07322.1.
PIRiT02433.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38124 mRNA. Translation: BAA07322.1.
PIRiT02433.

3D structure databases

ProteinModelPortaliQ40477.
SMRiQ40477. Positions 26-83.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.30.730.10. 1 hit.
InterProiIPR001471. AP2/ERF_dom.
IPR016177. DNA-bd_dom.
[Graphical view]
PfamiPF00847. AP2. 1 hit.
[Graphical view]
PRINTSiPR00367. ETHRSPELEMNT.
SMARTiSM00380. AP2. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS51032. AP2_ERF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Ethylene-inducible DNA binding proteins that interact with an ethylene responsive element."
    Ohme-Takagi M., Shinshi H.
    Plant Cell 7:173-182(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INDUCTION, TISSUE SPECIFICITY.
    Strain: cv. Bright Yellow 4.
    Tissue: Leaf.
  2. "Immediate early induction of mRNAs for ethylene-responsive transcription factors in tobacco leaf strips after cutting."
    Suzuki K., Suzuki N., Ohme-Takagi M., Shinshi H.
    Plant J. 15:657-665(1998)
    Cited for: FUNCTION, INDUCTION.
  3. "Elicitor-responsive, ethylene-independent activation of GCC box-mediated transcription that is regulated by both protein phosphorylation and dephosphorylation in cultured tobacco cells."
    Yamamoto S., Suzuki K., Shinshi H.
    Plant J. 20:571-579(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  4. "Three ethylene-responsive transcription factors in tobacco with distinct transactivation functions."
    Ohta M., Ohme-Takagi M., Shinshi H.
    Plant J. 22:29-38(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "Expression of PR-5d and ERF genes in cultured tobacco cells and their NaCl stress-response."
    Koyama T., Kitajima S., Sato F.
    Biosci. Biotechnol. Biochem. 65:1270-1273(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "Wounding activates immediate early transcription of genes for ERFs in tobacco plants."
    Nishiuchi T., Suzuki K., Kitajima S., Sato F., Shinshi H.
    Plant Mol. Biol. 49:473-482(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. "Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast two-hybrid system with tobacco ERF3 as bait and its characterization of specific interaction."
    Koyama T., Okada T., Kitajima S., Ohme-Takagi M., Shinshi H., Sato F.
    J. Exp. Bot. 54:1175-1181(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PROBABLE UBIQUITINATION, INTERACTION WITH UBC2.

Entry informationi

Entry nameiERF4_TOBAC
AccessioniPrimary (citable) accession number: Q40477
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: November 1, 1996
Last modified: December 9, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Caution

Was named ERF3 but it corresponds to Arabidopsis ERF4.Curated

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.