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Protein

Ethylene-responsive transcription factor 4

Gene

ERF4

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Binds to the GCC-box pathogenesis-related promoter element. Involved in the regulation of gene expression by stress factors and by components of stress signal transduction pathways mediated by ethylene, that seems to depend on a protein kinase/phosphatase cascade, and to be influenced by methyl-jasmonate. May regulate other AtERFs (By similarity).By similarity5 Publications

Caution

Was named ERF3 but it corresponds to Arabidopsis ERF4.Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi26 – 83AP2/ERFPROSITE-ProRule annotationAdd BLAST58

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Repressor
Biological processEthylene signaling pathway, Plant defense, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Ethylene-responsive transcription factor 4
Alternative name(s):
Ethylene-responsive element-binding factor 3
Short name:
EREBP-3
Ethylene-responsive element-binding factor 4 homolog
NtERF3
Gene namesi
Name:ERF4
Synonyms:ERF-3, ERF3
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana
Proteomesi
  • UP000084051 Componenti: Genome assembly

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001125561 – 225Ethylene-responsive transcription factor 4Add BLAST225

Post-translational modificationi

Probably negatively regulated by UBC2, that targets it to the ubiquitin proteasome pathway after ubiquitination and leads it to proteolysis.Curated

Keywords - PTMi

Ubl conjugation

Proteomic databases

PRIDEiQ40477

Expressioni

Tissue specificityi

Expressed in roots, in cultured cells and at low levels in buds and leaves. Highly expressed in cell culture, especially during the exponential phase.2 Publications

Inductioni

Strongly induced by ethephon (ethylene-releasing compound) in buds and to lower extent in leaves. Strongly induced by cycloheximide and mechanical stimuli. Wounding leads to a both local and systemical transient expression, independently of ethylene, and through a de-novo-protein-synthesis-independent regulation. Not influenced by methyl-jasmonate. Induction by purified xylanase from Trichoderma viride (TvX) and another elicitor from Phytophthora infestans (PiE), that appears to be mediated by a protein kinase cascade, and to be negatively regulated by protein phosphatases.4 Publications

Interactioni

Subunit structurei

Interacts with UBC2.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ40477
SMRiQ40477
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni92 – 196Interaction with UBC21 PublicationAdd BLAST105

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi215 – 221EAR-like (transcriptional repression)Sequence analysis7

Domaini

The AP2/ERF domain binds specifically to the 5'-GCCGCC-3' motif. The affinity of this binding is higher if the seventh amino-acid of this domain is basic (By similarity).By similarity
Contains a slightly degenerated ERF-associated amphiphilic repression (EAR) motif, which may be involved in the activity of transcriptional repression.By similarity

Sequence similaritiesi

Phylogenomic databases

KOiK09286

Family and domain databases

CDDicd00018 AP2, 1 hit
Gene3Di3.30.730.10, 1 hit
InterProiView protein in InterPro
IPR001471 AP2/ERF_dom
IPR036955 AP2/ERF_dom_sf
IPR016177 DNA-bd_dom_sf
PfamiView protein in Pfam
PF00847 AP2, 1 hit
PRINTSiPR00367 ETHRSPELEMNT
SMARTiView protein in SMART
SM00380 AP2, 1 hit
SUPFAMiSSF54171 SSF54171, 1 hit
PROSITEiView protein in PROSITE
PS51032 AP2_ERF, 1 hit

Sequencei

Sequence statusi: Complete.

Q40477-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVKNKVSNG NLKGGNVKTD GVKEVHYRGV RKRPWGRYAA EIRDPGKKSR
60 70 80 90 100
VWLGTFDTAE EAAKAYDTAA REFRGPKAKT NFPSPTENQS PSHSSTVESS
110 120 130 140 150
SGENGVHAPP HAPLELDLTR RLGSVAADGG DNCRRSGEVG YPIFHQQPTV
160 170 180 190 200
AVLPNGQPVL LFDSLWRAGV VNRPQPYHVT PMGFNGVNAG VGPTVSDSSS
210 220
AVEENQYDGK RGIDLDLNLA PPMEF
Length:225
Mass (Da):24,262
Last modified:November 1, 1996 - v1
Checksum:iBFF148E5B0B4EFAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38124 mRNA Translation: BAA07322.1
PIRiT02433
RefSeqiNP_001312182.1, NM_001325253.1
UniGeneiNta.1635

Genome annotation databases

GeneIDi107778042
KEGGinta:107778042

Similar proteinsi

Entry informationi

Entry nameiERF4_TOBAC
AccessioniPrimary (citable) accession number: Q40477
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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