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Protein

Zeaxanthin epoxidase, chloroplastic

Gene

ABA2

Organism
Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Converts zeaxanthin into antheraxanthin and subsequently violaxanthin. Involved in the epoxidation of zeaxanthin. Plays an important role in resistance to stresses, seed development and dormancy.

Catalytic activityi

Zeaxanthin + 2 NAD(P)H + 2 O2 = violaxanthin + 2 NAD(P)+ + 2 H2O.

Cofactori

FADCurated

Pathwayi: abscisate biosynthesis

This protein is involved in the pathway abscisate biosynthesis, which is part of Plant hormone biosynthesis.
View all proteins of this organism that are known to be involved in the pathway abscisate biosynthesis and in Plant hormone biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi81 – 10929FADSequence analysisAdd
BLAST
Nucleotide bindingi359 – 37214FADSequence analysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Abscisic acid biosynthesis, Stress response

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-2602.
BRENDAi1.14.13.90. 3640.
UniPathwayiUPA00090.

Names & Taxonomyi

Protein namesi
Recommended name:
Zeaxanthin epoxidase, chloroplastic (EC:1.14.13.90)
Gene namesi
Name:ABA2
OrganismiNicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)
Taxonomic identifieri4092 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5050ChloroplastSequence analysisAdd
BLAST
Chaini51 – 663613Zeaxanthin epoxidase, chloroplasticPRO_0000020611Add
BLAST

Expressioni

Tissue specificityi

Higher expression in leaves than in roots.

Inductioni

By drought stress; in roots.

Structurei

3D structure databases

ProteinModelPortaliQ40412.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini547 – 61165FHAPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FHA domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
3.50.50.60. 3 hits.
InterProiIPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
IPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
IPR017079. Zeaxanthin_epoxidase.
[Graphical view]
PfamiPF01494. FAD_binding_3. 2 hits.
PF00498. FHA. 1 hit.
[Graphical view]
PIRSFiPIRSF036989. Zeaxanthin_epoxidase. 1 hit.
SMARTiSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF51905. SSF51905. 2 hits.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q40412-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSTVFYTSV HPSTSAFSRK QLPLLISKDF PTELYHSLPC SRSLENGQIK
60 70 80 90 100
KVKGVVKATI AEAPATIPPT DLKKVPQKKL KVLVAGGGIG GLVFALAAKK
110 120 130 140 150
RGFDVLVFER DLSAIRGEGQ YRGPIQIQSN ALAALEAIDM DVAEDIMNAG
160 170 180 190 200
CITGQRINGL VDGVSGNWYC KFDTFTPAVE RGLPVTRVIS RMTLQQNLAR
210 220 230 240 250
AVGEDIIMNE SNVVNFEDDG EKVTVTLEDG QQYTGDLLVG ADGIRSKVRT
260 270 280 290 300
NLFGPSDVTY SGYTCYTGIA DFVPADIETV GYRVFLGHKQ YFVSSDVGGG
310 320 330 340 350
KMQWYAFHNE PAGGVDDPNG KKARLLKIFE GWCDNVIDLL VATDEDAILR
360 370 380 390 400
RDIYDRPPTF SWGKGRVTLL GDSVHAMQPN LGQGGCMAIE DSYQLALELD
410 420 430 440 450
KALSRSAESG TPVDIISSLR SYESSRKLRV GVIHGLARMA AIMASTYKAY
460 470 480 490 500
LGVGLGPLSF LTKFRIPHPG RVGGRFFIDL GMPLMLSWVL GGNGEKLEGR
510 520 530 540 550
IQHCRLSEKA NDQLRNWFED DDALERATDA EWLLLPAGNS NAALETLVLS
560 570 580 590 600
RDENMPCNIG SVSHANIPGK SVVIPLPQVS EMHARISYKG GAFFVTDLRS
610 620 630 640 650
EHGTWITDNE GRRYRASPNF PTRFHPSDII EFGSDKKAAF RVKVMKFPPK
660
TAAKEERQAV GAA
Length:663
Mass (Da):72,524
Last modified:November 1, 1996 - v1
Checksum:iD8D8BB4E73A661BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95732 mRNA. Translation: CAA65048.1.
PIRiS69548.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X95732 mRNA. Translation: CAA65048.1.
PIRiS69548.

3D structure databases

ProteinModelPortaliQ40412.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00090.
BioCyciMetaCyc:MONOMER-2602.
BRENDAi1.14.13.90. 3640.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
3.50.50.60. 3 hits.
InterProiIPR002938. FAD-bd.
IPR023753. FAD/NAD-binding_dom.
IPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
IPR017079. Zeaxanthin_epoxidase.
[Graphical view]
PfamiPF01494. FAD_binding_3. 2 hits.
PF00498. FHA. 1 hit.
[Graphical view]
PIRSFiPIRSF036989. Zeaxanthin_epoxidase. 1 hit.
SMARTiSM00240. FHA. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
SSF51905. SSF51905. 2 hits.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular identification of zeaxanthin epoxidase of Nicotiana plumbaginifolia, a gene involved in abscisic acid biosynthesis and corresponding to the ABA locus of Arabidopsis thaliana."
    Marin E., Nussaume L., Quesada A., Gonneau M., Sotta B., Hugueney P., Frey A., Marion-Poll A.
    EMBO J. 15:2331-2342(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Strain: cv. Viviani.
    Tissue: Seedling.
  2. "Expression studies of the zeaxanthin epoxidase gene in Nicotiana plumbaginifolia."
    Audran C., Borel C., Frey A., Sotta B., Meyer C., Simonneau T., Marion-Poll A.
    Plant Physiol. 118:1021-1028(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: cv. Viviani.

Entry informationi

Entry nameiABA2_NICPL
AccessioniPrimary (citable) accession number: Q40412
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1996
Last modified: November 11, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.