Reviewed, UniProtKB/Swiss-Prot Q40412 (ABA2_NICPL)

Names and origin

Protein names

Recommended name:
    Zeaxanthin epoxidase, chloroplastic
    EC=1.14.13.90

Gene names

Name:

ABA2

Organism

Nicotiana plumbaginifolia (Leadwort-leaved tobacco)

Taxonomic identifier

4092 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	663 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts zeaxanthin into antheraxanthin and subsequently violaxanthin. Involved in the epoxidation of zeaxanthin. Plays an important role in resistance to stresses, seed development and dormancy.
Catalytic activity	Zeaxanthin + NAD(P)H + O(2) = antheraxanthin + NAD(P)(+) + H(2)O. Antheraxanthin + NAD(P)H + O(2) = violaxanthin + NAD(P)(+) + H(2)O.
Cofactor	FAD Potential.
Pathway	Plant hormone biosynthesis; abscisic acid biosynthesis. Lipid metabolism; steroid biosynthesis.
Subcellular location	Plastid › chloroplast membrane; Peripheral membrane protein. Plastid › chloroplast thylakoid membrane; Peripheral membrane protein.
Tissue specificity	Higher expression in leaves than in roots.
Induction	By drought stress; in roots.
Sequence similarities	Contains 1 FHA domain.

Ontologies

Keywords
Biological process	Abscisic acid biosynthesis Stress response
Cellular component	Chloroplast Membrane Plastid
Domain	Transit peptide
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	abscisic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	zeaxanthin epoxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 50	50	Chloroplast Potential
	Chain	51 – 663	613	Zeaxanthin epoxidase, chloroplastic		PRO_0000020611
Regions
	Domain	547 – 611	65	FHA
	Nucleotide binding	81 – 109	29	FAD Potential
	Nucleotide binding	359 – 372	14	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q40412-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: D8D8BB4E73A661BA	FASTA	663	72,524
	10 20 30 40 50 60 MYSTVFYTSV HPSTSAFSRK QLPLLISKDF PTELYHSLPC SRSLENGQIK KVKGVVKATI 70 80 90 100 110 120 AEAPATIPPT DLKKVPQKKL KVLVAGGGIG GLVFALAAKK RGFDVLVFER DLSAIRGEGQ 130 140 150 160 170 180 YRGPIQIQSN ALAALEAIDM DVAEDIMNAG CITGQRINGL VDGVSGNWYC KFDTFTPAVE 190 200 210 220 230 240 RGLPVTRVIS RMTLQQNLAR AVGEDIIMNE SNVVNFEDDG EKVTVTLEDG QQYTGDLLVG 250 260 270 280 290 300 ADGIRSKVRT NLFGPSDVTY SGYTCYTGIA DFVPADIETV GYRVFLGHKQ YFVSSDVGGG 310 320 330 340 350 360 KMQWYAFHNE PAGGVDDPNG KKARLLKIFE GWCDNVIDLL VATDEDAILR RDIYDRPPTF 370 380 390 400 410 420 SWGKGRVTLL GDSVHAMQPN LGQGGCMAIE DSYQLALELD KALSRSAESG TPVDIISSLR 430 440 450 460 470 480 SYESSRKLRV GVIHGLARMA AIMASTYKAY LGVGLGPLSF LTKFRIPHPG RVGGRFFIDL 490 500 510 520 530 540 GMPLMLSWVL GGNGEKLEGR IQHCRLSEKA NDQLRNWFED DDALERATDA EWLLLPAGNS 550 560 570 580 590 600 NAALETLVLS RDENMPCNIG SVSHANIPGK SVVIPLPQVS EMHARISYKG GAFFVTDLRS 610 620 630 640 650 660 EHGTWITDNE GRRYRASPNF PTRFHPSDII EFGSDKKAAF RVKVMKFPPK TAAKEERQAV GAA

References

[1]

"Molecular identification of zeaxanthin epoxidase of Nicotiana plumbaginifolia, a gene involved in abscisic acid biosynthesis and corresponding to the ABA locus of Arabidopsis thaliana." Marin E., Nussaume L., Quesada A., Gonneau M., Sotta B., Hugueney P., Frey A., Marion-Poll A. EMBO J. 15:2331-2342(1996) [PubMed: 8665840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Strain: cv. Viviani. Tissue: Seedling.

[2]

"Expression studies of the zeaxanthin epoxidase gene in Nicotiana plumbaginifolia." Audran C., Borel C., Frey A., Sotta B., Meyer C., Simonneau T., Marion-Poll A. Plant Physiol. 118:1021-1028(1998) [PubMed: 9808747] [Abstract] Cited for: CHARACTERIZATION. Strain: cv. Viviani.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X95732 mRNA. Translation: CAA65048.1.
PIR	S69548.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MON-2602.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR000253. FHA. IPR003042. Rng_hydrolase. IPR017079. Zeaxanthin_epoxidase. [Graphical view]
Gene3D	G3DSA:2.60.200.20. FHA. 1 hit.
Pfam	PF01266. DAO. 1 hit. PF00498. FHA. 1 hit. [Graphical view]
PIRSF	PIRSF036989. Zeaxanthin_epoxidase. 1 hit.
PRINTS	PR00420. RNGMNOXGNASE.
SMART	SM00240. FHA. 1 hit. [Graphical view]
PROSITE	PS50006. FHA_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABA2_NICPL

Accession

Primary (citable) accession number: Q40412

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	November 4, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)