Q40401 (CALR_NICPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calreticulin

Gene names

Name:

CAL1

Organism

Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco)

Taxonomic identifier

4092 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin may interact transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER By similarity.
Subcellular location	Endoplasmic reticulum lumen By similarity.
Domain	Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity By similarity. The interaction with glycans occurs through a binding site in the globular lectin domain By similarity. The zinc binding sites are localized to the N-domain By similarity.
Sequence similarities	Belongs to the calreticulin family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum
Domain	Repeat Signal
Ligand	Calcium Lectin Metal-binding Zinc
Molecular function	Chaperone
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	protein folding Inferred from electronic annotation. Source: InterPro
Cellular_component	endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 416	389	Calreticulin		PRO_0000004192
Regions
	Repeat	199 – 210	12	1-1
	Repeat	218 – 229	12	1-2
	Repeat	235 – 246	12	1-3
	Repeat	253 – 264	12	1-4
	Repeat	268 – 278	11	2-1
	Repeat	282 – 292	11	2-2
	Repeat	296 – 306	11	2-3
	Region	199 – 264	66	4 X approximate repeats
	Region	268 – 306	39	3 X approximate repeats
	Motif	413 – 416	4	Prevents secretion from ER Potential
	Compositional bias	360 – 411	52	Asp/Glu/Lys-rich
Sites
	Binding site	117	1	Carbohydrate By similarity
	Binding site	119	1	Carbohydrate By similarity
	Binding site	136	1	Carbohydrate By similarity
	Binding site	143	1	Carbohydrate By similarity
	Binding site	326	1	Carbohydrate By similarity
Amino acid modifications
	Glycosylation	59	1	N-linked (GlcNAc...) Potential
	Glycosylation	159	1	N-linked (GlcNAc...) Potential
	Disulfide bond	113 ↔ 145		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q40401 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5026F3152B8828C0	FASTA	416	47,481
	10 20 30 40 50 60 MATQRRANPS SLHLITVFSL LVAVVSAEVF FEESFNDGWE SRWVKSEWKK DENMAGEWNH 70 80 90 100 110 120 TSGKWNGDAN DKGIQTSEDY RFYAISAEFP EFSNKGKNLV FQFSVKHEQK LDCGGGYMKL 130 140 150 160 170 180 LSGDVDQKKF GGDTPYSIMF GPDICGYSTK KVHAILTYND TNHLIKKEVP CETDQLTHVY 190 200 210 220 230 240 TFILRPDATY SILIDNVEKQ SGSLYSDWDL LPPKTIKDPS AKKPEDWDEK EFIDDPEDKK 250 260 270 280 290 300 PEGYDDIPEE ITDPDAKKPE DWDDEEDGEW TAPTIPNPEY KGPWKPKKIK NPNYKGKWKA 310 320 330 340 350 360 PLIDNPDFKD DPDLYVFPKL KYVGVELWQV KSGTLFDNIV ICDDPEYAKA IAEETWGKQK 370 380 390 400 410 DAEKAAFEEA EKKREEEESK AAPADSDAEE DDDADDDSDD ADDKSESKDD EAHDEL

References

[1]

"Calreticulin expression in plant cells: developmental regulation, tissue specificity and intracellular distribution." Borisjuk N., Sitailo L., Adler K., Malysheva L., Tewes A., Borisjuk L., Manteuffel R. Planta 206:504-514(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71395 mRNA. Translation: CAA95999.1.
PIR	T16968.
3D structure databases
ProteinModelPortal	Q40401.
SMR	Q40401. Positions 214-314.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.10.250.10. 1 hit. 2.60.120.200. 1 hit.
InterPro	IPR001580. Calret/calnex. IPR018124. Calret/calnex_CS. IPR009169. Calreticulin. IPR009033. Calreticulin/calnexin_P_dom. IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. [Graphical view]
PANTHER	PTHR11073. PTHR11073. 1 hit.
Pfam	PF00262. Calreticulin. 1 hit. [Graphical view]
PIRSF	PIRSF002356. Calreticulin. 1 hit.
PRINTS	PR00626. CALRETICULIN.
SUPFAM	SSF63887. Calret_calnex_P. 1 hit. SSF49899. ConA_like_lec_gl. 1 hit.
PROSITE	PS00803. CALRETICULIN_1. 1 hit. PS00804. CALRETICULIN_2. 1 hit. PS00805. CALRETICULIN_REPEAT. 2 hits. PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALR_NICPL

Accession

Primary (citable) accession number: Q40401

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program