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Protein

TMV resistance protein N

Gene

N

Organism
Nicotiana glutinosa (Tobacco)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Disease resistance protein. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via a direct or indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi216 – 2238ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Hypersensitive response, Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
TMV resistance protein N
Gene namesi
Name:N
OrganismiNicotiana glutinosa (Tobacco)
Taxonomic identifieri35889 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi12 – 121Y → S or F: Partial loss of resistance.
Mutagenesisi46 – 461D → H: Loss of resistance. 1 Publication
Mutagenesisi46 – 461D → Y: No effect. 1 Publication
Mutagenesisi63 – 631I → M: Loss of resistance. 1 Publication
Mutagenesisi63 – 631I → V: No effect. 1 Publication
Mutagenesisi66 – 661S → A: No effect. 1 Publication
Mutagenesisi67 – 671Q → E or K: Partial loss of resistance. 1 Publication
Mutagenesisi82 – 821W → S or A: Partial loss of resistance. 1 Publication
Mutagenesisi138 – 1381I → V or F: Partial loss of resistance. 1 Publication
Mutagenesisi141 – 1411W → S or A: Partial loss of resistance. 1 Publication
Mutagenesisi142 – 1421R → K or S: Partial loss of resistance. 1 Publication
Mutagenesisi216 – 2161G → A, E, V or R: Loss of resistance. 1 Publication
Mutagenesisi218 – 2181G → P: No effect. 1 Publication
Mutagenesisi218 – 2181G → R: Loss of resistance. 1 Publication
Mutagenesisi218 – 2181G → V or S: Partial loss of resistance. 1 Publication
Mutagenesisi219 – 2191G → D: Loss of resistance. 1 Publication
Mutagenesisi219 – 2191G → V: Partial loss of resistance. 1 Publication
Mutagenesisi222 – 2221K → E or N: Loss of resistance. 1 Publication
Mutagenesisi223 – 2231T → A or N: Loss of resistance. 1 Publication
Mutagenesisi223 – 2231T → S: Partial loss of resistance. 1 Publication
Mutagenesisi301 – 3011D → H, N or Y: Loss of resistance. 1 Publication
Mutagenesisi325 – 3251R → Y or G: Loss of resistance. 1 Publication
Mutagenesisi619 – 6191P → A or T: Partial loss of resistance. 1 Publication
Mutagenesisi619 – 6191P → S: Loss of resistance. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11441144TMV resistance protein NPRO_0000212781Add
BLAST

Expressioni

Developmental stagei

Isoform 1 is more prevalent before and for 3 hours after tobacco mosaic virus (TMV) infection, while isoform 2 is more prevalent 4 to 8 hours after TMV infection.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
NRIP1B2C7Y63EBI-1804401,EBI-1809509From a different organism.

Protein-protein interaction databases

IntActiQ40392. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ40392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini10 – 151142TIRPROSITE-ProRule annotationAdd
BLAST
Domaini172 – 447276NB-ARCAdd
BLAST
Repeati597 – 61822LRR 1Add
BLAST
Repeati620 – 64223LRR 2Add
BLAST
Repeati643 – 66523LRR 3Add
BLAST
Repeati834 – 85421LRR 4Add
BLAST
Repeati859 – 88022LRR 5Add
BLAST
Repeati882 – 90423LRR 6Add
BLAST

Sequence similaritiesi

Belongs to the disease resistance NB-LRR family.Curated
Contains 6 LRR (leucine-rich) repeats.Curated
Contains 1 NB-ARC domain.Curated
Contains 1 TIR domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
3.40.50.300. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR000157. TIR_dom.
[Graphical view]
PfamiPF13855. LRR_8. 1 hit.
PF00931. NB-ARC. 1 hit.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 4 hits.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 3 hits.
SSF52200. SSF52200. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
PS50104. TIR. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q40392-1) [UniParc]FASTAAdd to basket

Also known as: N, Ns

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASSSSSSRW SYDVFLSFRG EDTRKTFTSH LYEVLNDKGI KTFQDDKRLE
60 70 80 90 100
YGATIPGELC KAIEESQFAI VVFSENYATS RWCLNELVKI MECKTRFKQT
110 120 130 140 150
VIPIFYDVDP SHVRNQKESF AKAFEEHETK YKDDVEGIQR WRIALNEAAN
160 170 180 190 200
LKGSCDNRDK TDADCIRQIV DQISSKLCKI SLSYLQNIVG IDTHLEKIES
210 220 230 240 250
LLEIGINGVR IMGIWGMGGV GKTTIARAIF DTLLGRMDSS YQFDGACFLK
260 270 280 290 300
DIKENKRGMH SLQNALLSEL LREKANYNNE EDGKHQMASR LRSKKVLIVL
310 320 330 340 350
DDIDNKDHYL EYLAGDLDWF GNGSRIIITT RDKHLIEKND IIYEVTALPD
360 370 380 390 400
HESIQLFKQH AFGKEVPNEN FEKLSLEVVN YAKGLPLALK VWGSLLHNLR
410 420 430 440 450
LTEWKSAIEH MKNNSYSGII DKLKISYDGL EPKQQEMFLD IACFLRGEEK
460 470 480 490 500
DYILQILESC HIGAEYGLRI LIDKSLVFIS EYNQVQMHDL IQDMGKYIVN
510 520 530 540 550
FQKDPGERSR LWLAKEVEEV MSNNTGTMAM EAIWVSSYSS TLRFSNQAVK
560 570 580 590 600
NMKRLRVFNM GRSSTHYAID YLPNNLRCFV CTNYPWESFP STFELKMLVH
610 620 630 640 650
LQLRHNSLRH LWTETKHLPS LRRIDLSWSK RLTRTPDFTG MPNLEYVNLY
660 670 680 690 700
QCSNLEEVHH SLGCCSKVIG LYLNDCKSLK RFPCVNVESL EYLGLRSCDS
710 720 730 740 750
LEKLPEIYGR MKPEIQIHMQ GSGIRELPSS IFQYKTHVTK LLLWNMKNLV
760 770 780 790 800
ALPSSICRLK SLVSLSVSGC SKLESLPEEI GDLDNLRVFD ASDTLILRPP
810 820 830 840 850
SSIIRLNKLI ILMFRGFKDG VHFEFPPVAE GLHSLEYLNL SYCNLIDGGL
860 870 880 890 900
PEEIGSLSSL KKLDLSRNNF EHLPSSIAQL GALQSLDLKD CQRLTQLPEL
910 920 930 940 950
PPELNELHVD CHMALKFIHY LVTKRKKLHR VKLDDAHNDT MYNLFAYTMF
960 970 980 990 1000
QNISSMRHDI SASDSLSLTV FTGQPYPEKI PSWFHHQGWD SSVSVNLPEN
1010 1020 1030 1040 1050
WYIPDKFLGF AVCYSRSLID TTAHLIPVCD DKMSRMTQKL ALSECDTESS
1060 1070 1080 1090 1100
NYSEWDIHFF FVPFAGLWDT SKANGKTPND YGIIRLSFSG EEKMYGLRLL
1110 1120 1130 1140
YKEGPEVNAL LQMRENSNEP TEHSTGIRRT QYNNRTSFYE LING
Length:1,144
Mass (Da):131,486
Last modified:November 1, 1996 - v1
Checksum:i1EC5B36D4C4BCBB4
GO
Isoform 2 (identifier: Q40392-2) [UniParc]FASTAAdd to basket

Also known as: Ntr, Nl

The sequence of this isoform differs from the canonical sequence as follows:
     617-652: HLPSLRRIDLSWSKRLTRTPDFTGMPNLEYVNLYQC → KKNNIAEKEGDGILIEFWGDLQWAFAVSTEDRSQLV
     653-1144: Missing.

Show »
Length:652
Mass (Da):75,262
Checksum:iDD559795EC7B3E27
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei617 – 65236HLPSL…NLYQC → KKNNIAEKEGDGILIEFWGD LQWAFAVSTEDRSQLV in isoform 2. CuratedVSP_010126Add
BLAST
Alternative sequencei653 – 1144492Missing in isoform 2. CuratedVSP_010127Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15605 Genomic DNA. Translation: AAA50763.1.
PIRiA54810.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15605 Genomic DNA. Translation: AAA50763.1.
PIRiA54810.

3D structure databases

ProteinModelPortaliQ40392.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ40392. 1 interaction.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.10140. 1 hit.
3.40.50.300. 1 hit.
3.80.10.10. 2 hits.
InterProiIPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR002182. NB-ARC.
IPR027417. P-loop_NTPase.
IPR000157. TIR_dom.
[Graphical view]
PfamiPF13855. LRR_8. 1 hit.
PF00931. NB-ARC. 1 hit.
PF01582. TIR. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 4 hits.
SM00255. TIR. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 3 hits.
SSF52200. SSF52200. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
PS50104. TIR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The product of the tobacco mosaic virus resistance gene N: similarity to toll and the interleukin-1 receptor."
    Whitham S., Dinesh-Kumar S.P., Choi D., Hehl R., Corr C., Baker B.J.
    Cell 78:1101-1115(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
  2. "Alternatively spliced N resistance gene transcripts: their possible role in tobacco mosaic virus resistance."
    Dinesh-Kumar S.P., Baker B.J.
    Proc. Natl. Acad. Sci. U.S.A. 97:1908-1913(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), DEVELOPMENTAL STAGE.
  3. "Structure-function analysis of the tobacco mosaic virus resistance gene N."
    Dinesh-Kumar S.P., Tham W.H., Baker B.J.
    Proc. Natl. Acad. Sci. U.S.A. 97:14789-14794(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASP-46; ILE-63; SER-66; GLN-67; TRP-82; ILE-138; TRP-141; ARG-142; GLY-216; GLY-218; GLY-219; LYS-222; THR-223; ASP-301; ARG-325 AND PRO-619.

Entry informationi

Entry nameiTMVRN_NICGU
AccessioniPrimary (citable) accession number: Q40392
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Isoform 1 and isoform 2 are necessary to confer complete resistance to TMV.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.