Q40353 (MMK2_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase homolog MMK2
EC=2.7.11.24

Gene names

Name:

MMK2

Organism

Medicago sativa (Alfalfa)

Taxonomic identifier

3879 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation By similarity.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-195 and Tyr-197, which activates the enzyme By similarity. Autophosphorylated.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological_process	MAPK cascade Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	Mitogen-activated protein kinase homolog MMK2		PRO_0000186318
Regions
	Domain	37 – 323	287	Protein kinase
	Nucleotide binding	43 – 51	9	ATP By similarity
	Motif	195 – 197	3	TXY
Sites
	Active site	163	1	Proton acceptor By similarity
	Binding site	66	1	ATP By similarity
Amino acid modifications
	Modified residue	195	1	Phosphothreonine By similarity
	Modified residue	197	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q40353 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 1903E2D43158A4EE	FASTA	371	42,794
	10 20 30 40 50 60 MSVESAENNI RGVPTHGGRY LQYNIYGNLF EVSRKYVPPI RSVGRGAYGI VCAAVNAETR 70 80 90 100 110 120 EEVAIKKIGN AFDNRIDAKR TLREIKLLRH MDHENVMSIK DIIRPPQKEN FNHVYIVSEL 130 140 150 160 170 180 MDTDLHQIIR SNQPMTDDHC RYFVYQLLRG LKYVHSANVL HRDLKPSNLL LNANCDLKIG 190 200 210 220 230 240 DFGLARTTSE TDFMTEYVVT RWYRAPELLL NCSDYTAAID IWSVGCILGE IVTRQPLFPG 250 260 270 280 290 300 RDYVHQLRLV TELIGSPDDA SLGFLRSENA RRYVRQLPQY PKQNFSARFP NMSPGAVDLL 310 320 330 340 350 360 EKMLIFDPSK RIKVDEALCH PYMAPLHDIN EEPVCARPFS FDFEEPMFTE EDIKELIWKE 370 SVRFNPDPPI N

References

[1]	"MMK2, a novel alfalfa MAP kinase, specifically complements the yeast MPK1 function." Jonak C., Kiegerl S., Lloyd C., Chan J., Hirt H. Mol. Gen. Genet. 248:686-694(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X82268 mRNA. Translation: CAA57719.1.
PIR	S60121.
3D structure databases
ProteinModelPortal	Q40353.
SMR	Q40353. Positions 40-361.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.7.11.24. 3078.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MMK2_MEDSA

Accession

Primary (citable) accession number: Q40353

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program