Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q40353 (MMK2_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase homolog MMK2

EC=2.7.11.24
Gene names
Name:MMK2
OrganismMedicago sativa (Alfalfa)
Taxonomic identifier3879 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Protein attributes

Sequence length371 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by tyrosine and threonine phosphorylation By similarity.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-195 and Tyr-197, which activates the enzyme By similarity. Autophosphorylated.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 371371Mitogen-activated protein kinase homolog MMK2
PRO_0000186318

Regions

Domain37 – 323287Protein kinase
Nucleotide binding43 – 519ATP By similarity
Motif195 – 1973TXY

Sites

Active site1631Proton acceptor By similarity
Binding site661ATP By similarity

Amino acid modifications

Modified residue1951Phosphothreonine By similarity
Modified residue1971Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q40353 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 1903E2D43158A4EE

FASTA37142,794
        10         20         30         40         50         60 
MSVESAENNI RGVPTHGGRY LQYNIYGNLF EVSRKYVPPI RSVGRGAYGI VCAAVNAETR 

        70         80         90        100        110        120 
EEVAIKKIGN AFDNRIDAKR TLREIKLLRH MDHENVMSIK DIIRPPQKEN FNHVYIVSEL 

       130        140        150        160        170        180 
MDTDLHQIIR SNQPMTDDHC RYFVYQLLRG LKYVHSANVL HRDLKPSNLL LNANCDLKIG 

       190        200        210        220        230        240 
DFGLARTTSE TDFMTEYVVT RWYRAPELLL NCSDYTAAID IWSVGCILGE IVTRQPLFPG 

       250        260        270        280        290        300 
RDYVHQLRLV TELIGSPDDA SLGFLRSENA RRYVRQLPQY PKQNFSARFP NMSPGAVDLL 

       310        320        330        340        350        360 
EKMLIFDPSK RIKVDEALCH PYMAPLHDIN EEPVCARPFS FDFEEPMFTE EDIKELIWKE 

       370 
SVRFNPDPPI N 

« Hide

References

[1]"MMK2, a novel alfalfa MAP kinase, specifically complements the yeast MPK1 function."
Jonak C., Kiegerl S., Lloyd C., Chan J., Hirt H.
Mol. Gen. Genet. 248:686-694(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Embryo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X82268 mRNA. Translation: CAA57719.1.
PIRS60121.

3D structure databases

ProteinModelPortalQ40353.
SMRQ40353. Positions 40-361.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA2.7.11.24. 3078.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMMK2_MEDSA
AccessionPrimary (citable) accession number: Q40353
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families