Reviewed, UniProtKB/Swiss-Prot Q40345 (IDHP_MEDSA)

Names and origin

Protein names	Recommended name:     Isocitrate dehydrogenase [NADP], chloroplastic       Short name=IDH     EC=1.1.1.42 Alternative name(s):     Oxalosuccinate decarboxylase     NADP(+)-specific ICDH     IDP
Organism	Medicago sativa (Alfalfa)
Taxonomic identifier	3879 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	433 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subcellular location	Plastid › chloroplastPotential.
Tissue specificity	Detected in all tissues examined.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 21	›21	Chloroplast By similarity
	Chain	22 – 433	412	Isocitrate dehydrogenase [NADP], chloroplastic		PRO_0000014427
Regions
	Nucleotide binding	98 – 100	3	NADP By similarity
	Nucleotide binding	333 – 338	6	NADP By similarity
	Region	117 – 123	7	Substrate binding By similarity
Sites
	Metal binding	275	1	Magnesium or manganese By similarity
	Metal binding	298	1	Magnesium or manganese By similarity
	Binding site	100	1	Substrate By similarity
	Binding site	105	1	NADP By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	283	1	NADP By similarity
	Binding site	351	1	NADP; via amide nitrogen and carbonyl oxygen By similarity
	Site	162	1	Critical for catalysis By similarity
	Site	235	1	Critical for catalysis By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q40345-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E4421317B8A2CFE3	FASTA	433	48,383
	10 20 30 40 50 60 QFSPNLSFSA FFPIITFTTA TMGFQKIKVA NPIVEMDGDE MTRIIWKYIK DKLIFPFVEL 70 80 90 100 110 120 DIKYFDLGLP YRDETNDKVT VESAEATLKY NVAIKCATIT PDEARVKEFG LKSMWRSPNG 130 140 150 160 170 180 TIRNILNGTV FREPIICKNI PRLIPGWTKP ICIGRHAFGD QYRATDSVIK GPGKLKLVFV 190 200 210 220 230 240 PEGQGETTDL EVYNFTGEGG VALAMYNTDE SIRSFAEASM AVALEKKWPL YLSTKNTILK 250 260 270 280 290 300 KYDGRFKDIF QEVYEAGWKS KYEAAGIWYE HRLIDDMVAY ALKSEGGYVW ACKNYDGDVQ 310 320 330 340 350 360 SDFLAQGFGS LGLMTSVLVC PDGKTIEAEA AHGTVTRHFR VHQKGGETST NSIASIFAWT 370 380 390 400 410 420 RGLAHRAKLD DNATLLDFTE KLEAACIGVV ESGKMTKDLA LILHGSKLSR EHYLNTEEFI 430 DAVAAELKTK ISA

References

[1]	"Molecular characterization and expression of an isocitrate dehydrogenase from alfalfa (Medicago sativa L)." Shorrosh B.S., Dixon R.A. Plant Mol. Biol. 20:801-807(1992) [PubMed: 1281435] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M93672 mRNA. Translation: AAA32656.1.
PIR	S28423. T09619.
3D structure databases
HSSP	HSSP built from PDB template 1LWD based on UniProtKB P33198.
ModBase	Search...
Family and domain databases
InterPro	IPR004790. IsoCit_DHase_NADP-dep_euk. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
ProDom	PD328597. Nadp_idh_plant. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDHP_MEDSA

Accession

Primary (citable) accession number: Q40345

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)