Isocitrate dehydrogenase [NADP], chloroplastic precursor - Medicago sativa (Alfalfa)

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Reviewed, UniProtKB/Swiss-Prot Q40345 (IDHP_MEDSA)

Last modified June 16, 2009. Version 53. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names	Recommended name: Isocitrate dehydrogenase [NADP], chloroplastic Short name=IDH EC=1.1.1.42 Alternative name(s): Oxalosuccinate decarboxylase NADP(+)-specific ICDH IDP
Organism	Medicago sativa (Alfalfa)
Taxonomic identifier	3879 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	433 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Isocitrate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH. Oxalosuccinate + NADP⁺ = 2-oxoglutarate + CO₂ + NADPH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Subcellular location	Plastid › chloroplast Potential.
Tissue specificity	Detected in all tissues examined.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium Manganese Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

›21

Chloroplast By similarity

Chain

412

Isocitrate dehydrogenase [NADP], chloroplastic

PRO_0000014427

Regions

Nucleotide binding

3

NADP By similarity

Nucleotide binding

6

NADP By similarity

Region

7

Substrate binding By similarity

Sites

Metal binding

1

Magnesium or manganese By similarity

Metal binding

1

Magnesium or manganese By similarity

Binding site

1

Substrate By similarity

Binding site

1

NADP By similarity

Binding site

1

Substrate By similarity

Binding site

1

Substrate By similarity

Binding site

1

NADP By similarity

Binding site

1

NADP; via amide nitrogen and carbonyl oxygen By similarity

Site

1

Critical for catalysis By similarity

Site

1

Critical for catalysis By similarity

Experimental info

Non-terminal residue

1

Sequences

Sequence

Length

Mass (Da)

Tools

Q40345-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E4421317B8A2CFE3
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433

48,383

        10         20         30         40         50         60 
QFSPNLSFSA FFPIITFTTA TMGFQKIKVA NPIVEMDGDE MTRIIWKYIK DKLIFPFVEL 

        70         80         90        100        110        120 
DIKYFDLGLP YRDETNDKVT VESAEATLKY NVAIKCATIT PDEARVKEFG LKSMWRSPNG 

       130        140        150        160        170        180 
TIRNILNGTV FREPIICKNI PRLIPGWTKP ICIGRHAFGD QYRATDSVIK GPGKLKLVFV 

       190        200        210        220        230        240 
PEGQGETTDL EVYNFTGEGG VALAMYNTDE SIRSFAEASM AVALEKKWPL YLSTKNTILK 

       250        260        270        280        290        300 
KYDGRFKDIF QEVYEAGWKS KYEAAGIWYE HRLIDDMVAY ALKSEGGYVW ACKNYDGDVQ 

       310        320        330        340        350        360 
SDFLAQGFGS LGLMTSVLVC PDGKTIEAEA AHGTVTRHFR VHQKGGETST NSIASIFAWT 

       370        380        390        400        410        420 
RGLAHRAKLD DNATLLDFTE KLEAACIGVV ESGKMTKDLA LILHGSKLSR EHYLNTEEFI 

       430 
DAVAAELKTK ISA

References

[1]	"Molecular characterization and expression of an isocitrate dehydrogenase from alfalfa (Medicago sativa L)." Shorrosh B.S., Dixon R.A. Plant Mol. Biol. 20:801-807(1992) [PubMed: 1281435] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
	M93672 mRNA. Translation: AAA32656.1.
PIR	S28423. T09619.
3D structure databases
HSSP	HSSP built from PDB template 1LWD based on UniProtKB P33198.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.42. 815.
Family and domain databases
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004790. Isocitrate_DH_NADP-dep_euk. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11822. IDH_NADP_euk. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000108. IDH_NADP. 1 hit.
ProDom	PD328597. Nadp_idh_plant. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00127. nadp_idh_euk. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IDHP_MEDSA

Accession

Primary (citable) accession number: Q40345

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents