Reviewed,
UniProtKB/Swiss-Prot Q40345 (IDHP_MEDSA)
Last modified
November 25, 2008.
Version 50.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Isocitrate dehydrogenase [NADP], chloroplastic Short name=IDH EC=1.1.1.42 Alternative name(s): Oxalosuccinate decarboxylase NADP(+)-specific ICDH IDP |
| Organism | Medicago sativa (Alfalfa) |
| Taxonomic identifier | 3879 [NCBI] |
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago |
Protein attributes
| Sequence length | 433 AA. |
| Sequence status | Fragment. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Catalytic activity | Isocitrate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. Oxalosuccinate + NADP(+) = 2-oxoglutarate + CO(2) + NADPH. |
| Cofactor | Binds 1 magnesium or manganese ion per subunit By similarity. |
| Subcellular location | Plastid › chloroplastPotential. |
| Tissue specificity | Detected in all tissues examined. |
| Sequence similarities | Belongs to the isocitrate and isopropylmalate dehydrogenases family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Glyoxylate bypass Tricarboxylic acid cycle |
| Cellular component | Chloroplast Plastid |
| Domain | Transit peptide |
| Ligand | Magnesium Manganese Metal-binding NADP |
| Molecular function | Oxidoreductase |
Gene Ontology (GO) | |
| Biological process | glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW isocitrate metabolic processInferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycleInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | chloroplast Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | isocitrate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: InterPro magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW manganese ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | ‹1 – 21 | ›21 | Chloroplast By similarity | ||||||
| Chain | 22 – 433 | 412 | Isocitrate dehydrogenase [NADP], chloroplastic | PRO_0000014427 | |||||
Regions | |||||||||
| Nucleotide binding | 98 – 100 | 3 | NADP By similarity | ||||||
| Nucleotide binding | 333 – 338 | 6 | NADP By similarity | ||||||
| Region | 117 – 123 | 7 | Substrate binding By similarity | ||||||
Sites | |||||||||
| Metal binding | 275 | 1 | Magnesium or manganese By similarity | ||||||
| Metal binding | 298 | 1 | Magnesium or manganese By similarity | ||||||
| Binding site | 100 | 1 | Substrate By similarity | ||||||
| Binding site | 105 | 1 | NADP By similarity | ||||||
| Binding site | 132 | 1 | Substrate By similarity | ||||||
| Binding site | 155 | 1 | Substrate By similarity | ||||||
| Binding site | 283 | 1 | NADP By similarity | ||||||
| Binding site | 351 | 1 | NADP; via amide nitrogen and carbonyl oxygen By similarity | ||||||
| Site | 162 | 1 | Critical for catalysis By similarity | ||||||
| Site | 235 | 1 | Critical for catalysis By similarity | ||||||
Experimental info | |||||||||
| Non-terminal residue | 1 | 1 | |||||||
Sequences
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References
| [1] | "Molecular characterization and expression of an isocitrate dehydrogenase from alfalfa (Medicago sativa L)." Shorrosh B.S., Dixon R.A. Plant Mol. Biol. 20:801-807(1992) [PubMed: 1281435] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
Cross-references
Sequence databases | |
|---|---|
| M93672 mRNA. Translation: AAA32656.1. | |
| PIR | S28423. T09619. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1LWD based on UniProtKB P33198. |
| ModBase | Search... |
Family and domain databases | |
| InterPro | IPR004790. IsoCit_DHase_NADP-dep_euk. IPR001804. IsoCit_IM_DHase. [Graphical view] |
| Gene3D | G3DSA:3.40.718.10. IDH_IMDH. 1 hit. |
| PANTHER | PTHR11822. IDH_NADP_euk. 1 hit. |
| Pfam | PF00180. Iso_dh. 1 hit. [Graphical view] |
| PIRSF | PIRSF000108. IDH_NADP. 1 hit. |
| ProDom | PD328597. Nadp_idh_plant. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| TIGRFAMs | TIGR00127. nadp_idh_euk. 1 hit. |
| PROSITE | PS00470. IDH_IMDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | IDHP_MEDSA | ||||||||
| Accession | Primary (citable) accession number: Q40345 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||

Clusters with


