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Q40345

- IDHP_MEDSA

UniProt

Q40345 - IDHP_MEDSA

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Protein

Isocitrate dehydrogenase [NADP], chloroplastic

Gene
N/A
Organism
Medicago sativa (Alfalfa)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001Substrate By similarity
Binding sitei105 – 1051NADP By similarity
Binding sitei132 – 1321Substrate By similarity
Binding sitei155 – 1551Substrate By similarity
Sitei162 – 1621Critical for catalysis By similarity
Sitei235 – 2351Critical for catalysis By similarity
Metal bindingi275 – 2751Magnesium or manganese By similarity
Binding sitei283 – 2831NADP By similarity
Metal bindingi298 – 2981Magnesium or manganese By similarity
Binding sitei351 – 3511NADP; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi98 – 1003NADP By similarity
Nucleotide bindingi333 – 3386NADP By similarity

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. isocitrate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP], chloroplastic (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
OrganismiMedicago sativa (Alfalfa)
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Subcellular locationi

Plastidchloroplast Reviewed prediction

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei‹1 – 21›21Chloroplast By similarityAdd
BLAST
Chaini22 – 433412Isocitrate dehydrogenase [NADP], chloroplasticPRO_0000014427Add
BLAST

Proteomic databases

PRIDEiQ40345.
ProMEXiQ40345.

Expressioni

Tissue specificityi

Detected in all tissues examined.

Structurei

3D structure databases

ProteinModelPortaliQ40345.
SMRiQ40345. Positions 25-427.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni117 – 1237Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q40345-1 [UniParc]FASTAAdd to Basket

« Hide

QFSPNLSFSA FFPIITFTTA TMGFQKIKVA NPIVEMDGDE MTRIIWKYIK    50
DKLIFPFVEL DIKYFDLGLP YRDETNDKVT VESAEATLKY NVAIKCATIT 100
PDEARVKEFG LKSMWRSPNG TIRNILNGTV FREPIICKNI PRLIPGWTKP 150
ICIGRHAFGD QYRATDSVIK GPGKLKLVFV PEGQGETTDL EVYNFTGEGG 200
VALAMYNTDE SIRSFAEASM AVALEKKWPL YLSTKNTILK KYDGRFKDIF 250
QEVYEAGWKS KYEAAGIWYE HRLIDDMVAY ALKSEGGYVW ACKNYDGDVQ 300
SDFLAQGFGS LGLMTSVLVC PDGKTIEAEA AHGTVTRHFR VHQKGGETST 350
NSIASIFAWT RGLAHRAKLD DNATLLDFTE KLEAACIGVV ESGKMTKDLA 400
LILHGSKLSR EHYLNTEEFI DAVAAELKTK ISA 433
Length:433
Mass (Da):48,383
Last modified:November 1, 1996 - v1
Checksum:iE4421317B8A2CFE3
GO

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93672 mRNA. Translation: AAA32656.1.
PIRiS28423.
T09619.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93672 mRNA. Translation: AAA32656.1 .
PIRi S28423.
T09619.

3D structure databases

ProteinModelPortali Q40345.
SMRi Q40345. Positions 25-427.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q40345.
ProMEXi Q40345.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11822. PTHR11822. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization and expression of an isocitrate dehydrogenase from alfalfa (Medicago sativa L)."
    Shorrosh B.S., Dixon R.A.
    Plant Mol. Biol. 20:801-807(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiIDHP_MEDSA
AccessioniPrimary (citable) accession number: Q40345
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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