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Q40345

- IDHP_MEDSA

UniProt

Q40345 - IDHP_MEDSA

Protein

Isocitrate dehydrogenase [NADP], chloroplastic

Gene
N/A
Organism
Medicago sativa (Alfalfa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei100 – 1001SubstrateBy similarity
    Binding sitei105 – 1051NADPBy similarity
    Binding sitei132 – 1321SubstrateBy similarity
    Binding sitei155 – 1551SubstrateBy similarity
    Sitei162 – 1621Critical for catalysisBy similarity
    Sitei235 – 2351Critical for catalysisBy similarity
    Metal bindingi275 – 2751Magnesium or manganeseBy similarity
    Binding sitei283 – 2831NADPBy similarity
    Metal bindingi298 – 2981Magnesium or manganeseBy similarity
    Binding sitei351 – 3511NADP; via amide nitrogen and carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi98 – 1003NADPBy similarity
    Nucleotide bindingi333 – 3386NADPBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-KW
    2. isocitrate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP], chloroplastic (EC:1.1.1.42)
    Short name:
    IDH
    Alternative name(s):
    IDP
    NADP(+)-specific ICDH
    Oxalosuccinate decarboxylase
    OrganismiMedicago sativa (Alfalfa)
    Taxonomic identifieri3879 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

    Subcellular locationi

    Plastidchloroplast Curated

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei‹1 – 21›21ChloroplastBy similarityAdd
    BLAST
    Chaini22 – 433412Isocitrate dehydrogenase [NADP], chloroplasticPRO_0000014427Add
    BLAST

    Proteomic databases

    PRIDEiQ40345.
    ProMEXiQ40345.

    Expressioni

    Tissue specificityi

    Detected in all tissues examined.

    Structurei

    3D structure databases

    ProteinModelPortaliQ40345.
    SMRiQ40345. Positions 25-427.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni117 – 1237Substrate bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q40345-1 [UniParc]FASTAAdd to Basket

    « Hide

    QFSPNLSFSA FFPIITFTTA TMGFQKIKVA NPIVEMDGDE MTRIIWKYIK    50
    DKLIFPFVEL DIKYFDLGLP YRDETNDKVT VESAEATLKY NVAIKCATIT 100
    PDEARVKEFG LKSMWRSPNG TIRNILNGTV FREPIICKNI PRLIPGWTKP 150
    ICIGRHAFGD QYRATDSVIK GPGKLKLVFV PEGQGETTDL EVYNFTGEGG 200
    VALAMYNTDE SIRSFAEASM AVALEKKWPL YLSTKNTILK KYDGRFKDIF 250
    QEVYEAGWKS KYEAAGIWYE HRLIDDMVAY ALKSEGGYVW ACKNYDGDVQ 300
    SDFLAQGFGS LGLMTSVLVC PDGKTIEAEA AHGTVTRHFR VHQKGGETST 350
    NSIASIFAWT RGLAHRAKLD DNATLLDFTE KLEAACIGVV ESGKMTKDLA 400
    LILHGSKLSR EHYLNTEEFI DAVAAELKTK ISA 433
    Length:433
    Mass (Da):48,383
    Last modified:November 1, 1996 - v1
    Checksum:iE4421317B8A2CFE3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93672 mRNA. Translation: AAA32656.1.
    PIRiS28423.
    T09619.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M93672 mRNA. Translation: AAA32656.1 .
    PIRi S28423.
    T09619.

    3D structure databases

    ProteinModelPortali Q40345.
    SMRi Q40345. Positions 25-427.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q40345.
    ProMEXi Q40345.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization and expression of an isocitrate dehydrogenase from alfalfa (Medicago sativa L)."
      Shorrosh B.S., Dixon R.A.
      Plant Mol. Biol. 20:801-807(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiIDHP_MEDSA
    AccessioniPrimary (citable) accession number: Q40345
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3