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Q40345 (IDHP_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP], chloroplastic

Short name=IDH
EC=1.1.1.42
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
OrganismMedicago sativa (Alfalfa)
Taxonomic identifier3879 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Protein attributes

Sequence length433 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subcellular location

Plastidchloroplast Potential.

Tissue specificity

Detected in all tissues examined.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide‹1 – 21›21Chloroplast By similarity
Chain22 – 433412Isocitrate dehydrogenase [NADP], chloroplastic
PRO_0000014427

Regions

Nucleotide binding98 – 1003NADP By similarity
Nucleotide binding333 – 3386NADP By similarity
Region117 – 1237Substrate binding By similarity

Sites

Metal binding2751Magnesium or manganese By similarity
Metal binding2981Magnesium or manganese By similarity
Binding site1001Substrate By similarity
Binding site1051NADP By similarity
Binding site1321Substrate By similarity
Binding site1551Substrate By similarity
Binding site2831NADP By similarity
Binding site3511NADP; via amide nitrogen and carbonyl oxygen By similarity
Site1621Critical for catalysis By similarity
Site2351Critical for catalysis By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
Q40345 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: E4421317B8A2CFE3

FASTA43348,383
        10         20         30         40         50         60 
QFSPNLSFSA FFPIITFTTA TMGFQKIKVA NPIVEMDGDE MTRIIWKYIK DKLIFPFVEL 

        70         80         90        100        110        120 
DIKYFDLGLP YRDETNDKVT VESAEATLKY NVAIKCATIT PDEARVKEFG LKSMWRSPNG 

       130        140        150        160        170        180 
TIRNILNGTV FREPIICKNI PRLIPGWTKP ICIGRHAFGD QYRATDSVIK GPGKLKLVFV 

       190        200        210        220        230        240 
PEGQGETTDL EVYNFTGEGG VALAMYNTDE SIRSFAEASM AVALEKKWPL YLSTKNTILK 

       250        260        270        280        290        300 
KYDGRFKDIF QEVYEAGWKS KYEAAGIWYE HRLIDDMVAY ALKSEGGYVW ACKNYDGDVQ 

       310        320        330        340        350        360 
SDFLAQGFGS LGLMTSVLVC PDGKTIEAEA AHGTVTRHFR VHQKGGETST NSIASIFAWT 

       370        380        390        400        410        420 
RGLAHRAKLD DNATLLDFTE KLEAACIGVV ESGKMTKDLA LILHGSKLSR EHYLNTEEFI 

       430 
DAVAAELKTK ISA 

« Hide

References

[1]"Molecular characterization and expression of an isocitrate dehydrogenase from alfalfa (Medicago sativa L)."
Shorrosh B.S., Dixon R.A.
Plant Mol. Biol. 20:801-807(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M93672 mRNA. Translation: AAA32656.1.
PIRS28423.
T09619.

3D structure databases

ProteinModelPortalQ40345.
SMRQ40345. Positions 25-427.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ40345.
ProMEXQ40345.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIDHP_MEDSA
AccessionPrimary (citable) accession number: Q40345
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families