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Q40345

- IDHP_MEDSA

UniProt

Q40345 - IDHP_MEDSA

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Protein

Isocitrate dehydrogenase [NADP], chloroplastic

Gene
N/A
Organism
Medicago sativa (Alfalfa)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Binds 1 magnesium or manganese ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001SubstrateBy similarity
Binding sitei105 – 1051NADPBy similarity
Binding sitei132 – 1321SubstrateBy similarity
Binding sitei155 – 1551SubstrateBy similarity
Sitei162 – 1621Critical for catalysisBy similarity
Sitei235 – 2351Critical for catalysisBy similarity
Metal bindingi275 – 2751Magnesium or manganeseBy similarity
Binding sitei283 – 2831NADPBy similarity
Metal bindingi298 – 2981Magnesium or manganeseBy similarity
Binding sitei351 – 3511NADP; via amide nitrogen and carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi98 – 1003NADPBy similarity
Nucleotide bindingi333 – 3386NADPBy similarity

GO - Molecular functioni

  1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro
  3. NAD binding Source: InterPro

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. isocitrate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP], chloroplastic (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
OrganismiMedicago sativa (Alfalfa)
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Subcellular locationi

Plastidchloroplast Curated

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei‹1 – 21›21ChloroplastBy similarityAdd
BLAST
Chaini22 – 433412Isocitrate dehydrogenase [NADP], chloroplasticPRO_0000014427Add
BLAST

Proteomic databases

PRIDEiQ40345.
ProMEXiQ40345.

Expressioni

Tissue specificityi

Detected in all tissues examined.

Structurei

3D structure databases

ProteinModelPortaliQ40345.
SMRiQ40345. Positions 25-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni117 – 1237Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q40345-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
QFSPNLSFSA FFPIITFTTA TMGFQKIKVA NPIVEMDGDE MTRIIWKYIK
60 70 80 90 100
DKLIFPFVEL DIKYFDLGLP YRDETNDKVT VESAEATLKY NVAIKCATIT
110 120 130 140 150
PDEARVKEFG LKSMWRSPNG TIRNILNGTV FREPIICKNI PRLIPGWTKP
160 170 180 190 200
ICIGRHAFGD QYRATDSVIK GPGKLKLVFV PEGQGETTDL EVYNFTGEGG
210 220 230 240 250
VALAMYNTDE SIRSFAEASM AVALEKKWPL YLSTKNTILK KYDGRFKDIF
260 270 280 290 300
QEVYEAGWKS KYEAAGIWYE HRLIDDMVAY ALKSEGGYVW ACKNYDGDVQ
310 320 330 340 350
SDFLAQGFGS LGLMTSVLVC PDGKTIEAEA AHGTVTRHFR VHQKGGETST
360 370 380 390 400
NSIASIFAWT RGLAHRAKLD DNATLLDFTE KLEAACIGVV ESGKMTKDLA
410 420 430
LILHGSKLSR EHYLNTEEFI DAVAAELKTK ISA
Length:433
Mass (Da):48,383
Last modified:November 1, 1996 - v1
Checksum:iE4421317B8A2CFE3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93672 mRNA. Translation: AAA32656.1.
PIRiS28423.
T09619.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M93672 mRNA. Translation: AAA32656.1 .
PIRi S28423.
T09619.

3D structure databases

ProteinModelPortali Q40345.
SMRi Q40345. Positions 25-427.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q40345.
ProMEXi Q40345.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.718.10. 1 hit.
InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view ]
PANTHERi PTHR11822. PTHR11822. 1 hit.
Pfami PF00180. Iso_dh. 1 hit.
[Graphical view ]
PIRSFi PIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
PROSITEi PS00470. IDH_IMDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular characterization and expression of an isocitrate dehydrogenase from alfalfa (Medicago sativa L)."
    Shorrosh B.S., Dixon R.A.
    Plant Mol. Biol. 20:801-807(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiIDHP_MEDSA
AccessioniPrimary (citable) accession number: Q40345
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3