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Protein

Isocitrate dehydrogenase [NADP], chloroplastic

Gene
N/A
Organism
Medicago sativa (Alfalfa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100SubstrateBy similarity1
Binding sitei105NADPBy similarity1
Binding sitei132SubstrateBy similarity1
Binding sitei155SubstrateBy similarity1
Sitei162Critical for catalysisBy similarity1
Sitei235Critical for catalysisBy similarity1
Metal bindingi275Magnesium or manganeseBy similarity1
Binding sitei283NADPBy similarity1
Metal bindingi298Magnesium or manganeseBy similarity1
Binding sitei351NADP; via amide nitrogen and carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi98 – 100NADPBy similarity3
Nucleotide bindingi333 – 338NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP], chloroplastic (EC:1.1.1.42)
Short name:
IDH
Alternative name(s):
IDP
NADP(+)-specific ICDH
Oxalosuccinate decarboxylase
OrganismiMedicago sativa (Alfalfa)
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei‹1 – 21ChloroplastBy similarityAdd BLAST›21
ChainiPRO_000001442722 – 433Isocitrate dehydrogenase [NADP], chloroplasticAdd BLAST412

Proteomic databases

PRIDEiQ40345.

Expressioni

Tissue specificityi

Detected in all tissues examined.

Structurei

3D structure databases

ProteinModelPortaliQ40345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni117 – 123Substrate bindingBy similarity7

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q40345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
QFSPNLSFSA FFPIITFTTA TMGFQKIKVA NPIVEMDGDE MTRIIWKYIK
60 70 80 90 100
DKLIFPFVEL DIKYFDLGLP YRDETNDKVT VESAEATLKY NVAIKCATIT
110 120 130 140 150
PDEARVKEFG LKSMWRSPNG TIRNILNGTV FREPIICKNI PRLIPGWTKP
160 170 180 190 200
ICIGRHAFGD QYRATDSVIK GPGKLKLVFV PEGQGETTDL EVYNFTGEGG
210 220 230 240 250
VALAMYNTDE SIRSFAEASM AVALEKKWPL YLSTKNTILK KYDGRFKDIF
260 270 280 290 300
QEVYEAGWKS KYEAAGIWYE HRLIDDMVAY ALKSEGGYVW ACKNYDGDVQ
310 320 330 340 350
SDFLAQGFGS LGLMTSVLVC PDGKTIEAEA AHGTVTRHFR VHQKGGETST
360 370 380 390 400
NSIASIFAWT RGLAHRAKLD DNATLLDFTE KLEAACIGVV ESGKMTKDLA
410 420 430
LILHGSKLSR EHYLNTEEFI DAVAAELKTK ISA
Length:433
Mass (Da):48,383
Last modified:November 1, 1996 - v1
Checksum:iE4421317B8A2CFE3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Non-terminal residuei11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93672 mRNA. Translation: AAA32656.1.
PIRiS28423.
T09619.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93672 mRNA. Translation: AAA32656.1.
PIRiS28423.
T09619.

3D structure databases

ProteinModelPortaliQ40345.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ40345.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
SMARTiSM01329. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIDHP_MEDSA
AccessioniPrimary (citable) accession number: Q40345
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: October 5, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.