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Protein
Submitted name:

4S-limonene synthase

Gene
N/A
Organism
Mentha spicata (Spearmint)
Status
Unreviewed-Annotation score: Annotation score: 1 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. magnesium ion binding Source: InterPro
  2. terpene synthase activity Source: InterPro
Complete GO annotation...

Keywords - Ligandi

MagnesiumSAAS annotation, Metal-bindingSAAS annotation

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-6782.

Names & Taxonomyi

Protein namesi
Submitted name:
4S-limonene synthaseImported
OrganismiMentha spicata (Spearmint)Imported
Taxonomic identifieri29719 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsLamialesLamiaceaeNepetoideaeMentheaeMentha

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 9999 PotentialImportedAdd
BLAST
Chaini100 – 5995004S-limonene synthaseImportedPRO_5000142170Add
BLAST

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ONGX-ray2.70A/B57-599[»]
2ONHX-ray2.70A/B57-599[»]
SMRiQ40322. Positions 58-598.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ40322.

Family & Domainsi

Keywords - Domaini

Transit peptideImported

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q40322-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALKVLSVAT QMAIPSNLTT CLQPSHFKSS PKLLSSTNSS SRSRLRVYCS
60 70 80 90 100
SSQLTTERRS GNYNPSRWDV NFIQSLLSDY KEDKHVIRAS ELVTLVKMEL
110 120 130 140 150
EKETDQIRQL ELIDDLQRMG LSDHFQNEFK EILSSIYLDH HYYKNPFPKE
160 170 180 190 200
ERDLYSTSLA FRLLREHGFQ VAQEVFDSFK NEEGEFKESL SDDTRGLLQL
210 220 230 240 250
YEASFLLTEG ETTLESAREF ATKFLEEKVN EGGVDGDLLT RIAYSLDIPL
260 270 280 290 300
HWRIKRPNAP VWIEWYRKRP DMNPVVLELA ILDLNIVQAQ FQEELKESFR
310 320 330 340 350
WWRNTGFVEK LPFARDRLVE CYFWNTGIIE PRQHASARIM MGKVNALITV
360 370 380 390 400
IDDIYDVYGT LEELEQFTDL IRRWDINSID QLPDYMQLCF LALNNFVDDT
410 420 430 440 450
SYDVMKEKGV NVIPYLRQSW VDLADKYMVE ARWFYGGHKP SLEEYLENSW
460 470 480 490 500
QSISGPCMLT HIFFRVTDSF TKETVDSLYK YHDLVRWSSF VLRLADDLGT
510 520 530 540 550
SVEEVSRGDV PKSLQCYMSD YNASEAEARK HVKWLIAEVW KKMNAERVSK
560 570 580 590
DSPFGKDFIG CAVDLGRMAQ LMYHNGDGHG TQHPIIHQQM TRTLFEPFA
Length:599
Mass (Da):69,843
Last modified:October 31, 1996 - v1
Checksum:i8BF53D23329CAF65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13459 mRNA. Translation: AAC37366.1.
PIRiA48863.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13459 mRNA. Translation: AAC37366.1.
PIRiA48863.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2ONGX-ray2.70A/B57-599[»]
2ONHX-ray2.70A/B57-599[»]
SMRiQ40322. Positions 58-598.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-6782.

Miscellaneous databases

EvolutionaryTraceiQ40322.

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
1.50.30.10. 1 hit.
InterProiIPR008949. Isoprenoid_synthase_dom.
IPR001906. Terpene_synth_N.
IPR005630. Terpene_synthase_metal-bd.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
[Graphical view]
PfamiPF01397. Terpene_synth. 1 hit.
PF03936. Terpene_synth_C. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF48576. SSF48576. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "4S-limonene synthase from the oil glands of spearmint (Mentha spicata). cDNA isolation, characterization, and bacterial expression of the catalytically active monoterpene cyclase."
    Colby S.M., Alonso W.R., Katahira E.J., McGarvey D.J., Croteau R.
    J. Biol. Chem. 268:23016-23024(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: LeafImported.
  2. "Structure of limonene synthase, a simple model for terpenoid cyclase catalysis."
    Hyatt D.C., Youn B., Zhao Y., Santhamma B., Coates R.M., Croteau R.B., Kang C.
    Proc. Natl. Acad. Sci. U.S.A. 104:5360-5365(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.70 ANGSTROMS) OF 57-599.

Entry informationi

Entry nameiQ40322_MENSP
AccessioniPrimary (citable) accession number: Q40322
Entry historyi
Integrated into UniProtKB/TrEMBL: October 31, 1996
Last sequence update: October 31, 1996
Last modified: March 31, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.