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Protein

Pectate lyase

Gene
N/A
Organism
Medicago sativa (Alfalfa)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.UniRule annotation

Cofactori

Ca2+UniRule annotationNote: Binds 1 Ca2+ ion. Required for its activity.UniRule annotation

Pathwayi: pectin degradation

This protein is involved in step 2 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pectinesterase (PER), Pectinesterase (PEF), Pectinesterase/pectinesterase inhibitor
  2. Pectate lyase
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotationImported

Keywords - Ligandi

CalciumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Protein family/group databases

CAZyiPL1. Polysaccharide Lyase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectate lyaseUniRule annotation (EC:4.2.2.2UniRule annotation)
OrganismiMedicago sativa (Alfalfa)Imported
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24UniRule annotationAdd BLAST24
ChainiPRO_500514329425 – 450Pectate lyaseUniRule annotationAdd BLAST426

Structurei

3D structure databases

ProteinModelPortaliQ40319.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini175 – 372Amb_allInterPro annotationAdd BLAST198

Sequence similaritiesi

Belongs to the polysaccharide lyase 1 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR007524. Pec_lyase_N.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
PF04431. Pec_lyase_N. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Q40319-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAIAMKISF ILLVLAITIP CLEGRIGEFD DYLKAQADLA RQIAFKSYVP
60 70 80 90 100
NPENITTEIN IHVHLAMEAA MKAEANDTRR ELMSQKSRGG KGRRARCMAT
110 120 130 140 150
NPIDSCWRCR KDWAENRQLL AKCAKGFGRR TTGGLGGRIY VVTDPSDNDM
160 170 180 190 200
VNPRPGTLRF GAVQRGPLWI IFQRSMVITL TQELMVSSDK TIDGRGANVQ
210 220 230 240 250
IRDGAGITMQ FVNNVIIHGL RIKNIKARNG GLIRDSFDHI GVRTRSDGDA
260 270 280 290 300
ISVFGSSNIW IDHISLSDCE DGLVDVIQGS TAVTISNCHM TKHNDVMLFG
310 320 330 340 350
ASDTYQDDKI MQITVAFNHF GQGLIQRMPR CRWGFFHVLN NDYTHWIMYA
360 370 380 390 400
IGGSSAPTIL SQGNRFIAPH NNAAKTVTHR DYAPESVWSK WQWRSEGDHF
410 420 430 440 450
MNGATFIQSG PPIKNLPFKK GFLMKPRHGS QANRLTRFSG ALNCVVGRPC
Length:450
Mass (Da):50,156
Last modified:November 1, 1996 - v1
Checksum:i38F83F9D37A35F46
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41472 Genomic DNA. Translation: AAA86241.1.
PIRiT09524.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U41472 Genomic DNA. Translation: AAA86241.1.
PIRiT09524.

3D structure databases

ProteinModelPortaliQ40319.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiPL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR007524. Pec_lyase_N.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
PF04431. Pec_lyase_N. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQ40319_MEDSA
AccessioniPrimary (citable) accession number: Q40319
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1996
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.