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Reviewed, UniProtKB/Swiss-Prot Q40312 (PGLR_MEDSA)

Last modified June 16, 2009. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Polygalacturonase
      Short name=PG
    EC=3.2.1.15
Alternative name(s):
    Pectinase
OrganismMedicago sativa (Alfalfa)
Taxonomic identifier3879 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

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Protein attributes

Sequence length421 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

May function in the depolymerization of the pectin in its walls during pollen tube elongation, or in that of the pistil during pollination By similarity.

Catalytic activity

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Subcellular location

Secreted By similarity. Secreted › cell wall By similarity.

Tissue specificity

Pollen specific.

Sequence similarities

Belongs to the glycosyl hydrolase 28 family.

Contains 4 PbH1 repeats.

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Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
   Cellular componentCell wall
Secreted
   DomainRepeat
Signal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

cell wall organization

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

extracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionpolygalacturonase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 421401Polygalacturonase
PRO_0000024810

Regions

Repeat178 – 20427PbH1 1
Repeat205 – 22622PbH1 2
Repeat258 – 27922PbH1 3
Repeat289 – 31022PbH1 4

Sites

Active site2191Proton donor By similarity
Active site2421 By similarity

Amino acid modifications

Glycosylation1561N-linked (GlcNAc...) Potential
Glycosylation1801N-linked (GlcNAc...) Potential
Glycosylation2651N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
Q40312-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 6E1599E2BD9C73DE

FASTA42143,953
        10         20         30         40         50         60 
MKFSTAIIVS FLFIADFCAA QSGVLDISKF GGKPNSDIGQ ALTSAWNEAC ASTTAAKIVI 

        70         80         90        100        110        120 
PAGTYQLNGI ELKGPCKAPI ELQVDGTIQA PADPSVIKGT EQWFKFLYMD HLTLSGKGVF 

       130        140        150        160        170        180 
DGQGATVYKK AAPASAWSGK NSNSKVFMNF GFNFVNNSIV RGVTSKDSKN FHVMVFGCKN 

       190        200        210        220        230        240 
ITFDGFTITA PGDSPNTDGI HMGKSTDVKI LNTNIGTGDD CVSIGDGSKQ ITVQGVNCGP 

       250        260        270        280        290        300 
GHGLSVGSLG KFTTEENVEG ITVKNCTLTA TDNGVRIKTW PDAPGTITVS DIHFEDITMT 

       310        320        330        340        350        360 
NVKNPVIIDQ EYYPWNQCSK KNPSKIKLSK ISFKNVKGTS GTAEGVVLIC SSAVPCDGVE 

       370        380        390        400        410        420 
LNNVDLKFNG APTTAKCTNV KPLVTGTAPV CQAPGAPAAS TTATPAASKT ATPAAGKSPA 


K

« Hide

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References

[1]Qiu X., Erickson L.
Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. C2-4.
Tissue: Pollen.

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Cross-references

Sequence databases

U20431 mRNA. Translation: AAA62286.1.
PIRT09398.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH28. Glycoside Hydrolase Family 28.

Enzyme and pathway databases

BRENDA3.2.1.15. 815.

Family and domain databases

InterProIPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
PfamPF00295. Glyco_hydro_28. 1 hit.
[Graphical view]
SMARTSM00710. PbH1. 4 hits.
[Graphical view]
PROSITEPS00502. POLYGALACTURONASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePGLR_MEDSA
AccessionPrimary (citable) accession number: Q40312
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents