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Q401N2 (ZACN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Zinc-activated ligand-gated ion channel
Alternative name(s):
Ligand-gated ion channel zinc-activated 1
Ligand-gated ion-channel receptor L2
Gene names
Name:ZACN
Synonyms:L2, LGICZ, LGICZ1, ZAC
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length412 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Zinc-activated ligand-gated ion channel. Ref.1

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.2.

Tissue specificity

Detected in pancreas, brain, liver, placenta, trachea, kidney, spinal cord, stomach and fetal brain. In the adult brain region expression is detected in the hippocampus, striatum, amygdala and thalamus. Ref.1 Ref.2

Post-translational modification

Glycosylated. Ref.2

Miscellaneous

The mouse and rat orthologous proteins do not exist.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. [View classification]

Caution

It is uncertain whether Met-1 or Met-2 is the initiator.

Sequence caution

The sequence AAI10598.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAO20969.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence EAW89365.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q401N2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q401N2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     182-266: AMELEFQAHV...RAIERIGYKV → GADRAGAAGL...VLSGSLRAPL
     267-412: Missing.
Isoform 3 (identifier: Q401N2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     75-126: DILRYTMSSM...TPRLTILEAL → SSVLPRATAL...PPCINPSGDI
     127-412: Missing.
Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 412387Zinc-activated ligand-gated ion channel
PRO_0000317165

Regions

Topological domain26 – 233208Extracellular Potential
Transmembrane234 – 25421Helical; Name=1; Potential
Topological domain255 – 26511Cytoplasmic Potential
Transmembrane266 – 28621Helical; Name=2; Potential
Topological domain287 – 29812Extracellular Potential
Transmembrane299 – 31921Helical; Name=3; Potential
Topological domain320 – 36849Cytoplasmic Potential
Transmembrane369 – 38921Helical; Name=4; Potential
Topological domain390 – 41223Extracellular Potential
Compositional bias224 – 31895Leu-rich

Amino acid modifications

Glycosylation411N-linked (GlcNAc...) Potential
Glycosylation551N-linked (GlcNAc...) Potential
Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation1701N-linked (GlcNAc...) Potential
PRO_5000052473
Disulfide bond157 ↔ 171 By similarity

Natural variations

Alternative sequence75 – 12652DILRY…ILEAL → SSVLPRATALHSHGDCSGSA PCSKARGSGCHGLTPGNHLK VQPPCINPSGDI in isoform 3.
VSP_030909
Alternative sequence127 – 412286Missing in isoform 3.
VSP_030910
Alternative sequence182 – 26685AMELE…IGYKV → GADRAGAAGLRRGGGRWGRG TPRSVVQGQGAGQGEGAKAD RRRTPRSVFRAVYPRLRRAA PALPLRPPLEWQPISVLSGS LRAPL in isoform 2.
VSP_030911
Alternative sequence267 – 412146Missing in isoform 2.
VSP_030912
Natural variant1521A → T. Ref.1 Ref.3
Corresponds to variant rs2257020 [ dbSNP | Ensembl ].
VAR_038483

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 5, 2008. Version 2.
Checksum: B894A28F5163723F

FASTA41245,816
        10         20         30         40         50         60 
MMALWSLLHL TFLGFSITLL LVHGQGFQGT AAIWPSLFNV NLSKKVQESI QIPNNGSAPL 

        70         80         90        100        110        120 
LVDVRVFVSN VFNVDILRYT MSSMLLLRLS WLDTRLAWNT SAHPRHAITL PWESLWTPRL 

       130        140        150        160        170        180 
TILEALWVDW RDQSPQARVD QDGHVKLNLA LATETNCNFE LLHFPRDHSN CSLSFYALSN 

       190        200        210        220        230        240 
TAMELEFQAH VVNEIVSVKR EYVVYDLKTQ VPPQQLVPCF QVTLRLKNTA LKSIIALLVP 

       250        260        270        280        290        300 
AEALLLADVC GGLLPLRAIE RIGYKVTLLL SYLVLHSSLV QALPSSSSCN PLLIYYFTIL 

       310        320        330        340        350        360 
LLLLFLSTIE TVLLAGLLAR GNLGAKSGPS PAPRGEQREH GNPGPHPAEE PSRGVKGSQR 

       370        380        390        400        410 
SWPETADRIF FLVYVVGVLC TQFVFAGIWM WAACKSDAAP GEAAPHGRRP RL 

« Hide

Isoform 2 [UniParc].

Checksum: 06C2C8684090C9C8
Show »

FASTA26629,616
Isoform 3 [UniParc].

Checksum: 9F87FF81BAB98B5F
Show »

FASTA12613,299

References

« Hide 'large scale' references
[1]"A novel class of ligand-gated ion channel is activated by Zn2+."
Davies P.A., Wang W., Hales T.G., Kirkness E.F.
J. Biol. Chem. 278:712-717(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION, VARIANT THR-152.
[2]"Cloning and expression of ligand-gated ion-channel receptor L2 in central nervous system."
Houtani T., Munemoto Y., Kase M., Sakuma S., Tsutsumi T., Sugimoto T.
Biochem. Biophys. Res. Commun. 335:277-285(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION.
Tissue: Brain.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT THR-152.
Tissue: Thymus.
[4]"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. expand/collapse author list , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF512521 mRNA. Translation: AAO20969.1. Different initiation.
AB223030 mRNA. Translation: BAE19924.1.
AK122638 mRNA. No translation available.
AC018665 Genomic DNA. No translation available.
CH471099 Genomic DNA. Translation: EAW89365.1. Different initiation.
CH471099 Genomic DNA. Translation: EAW89366.1.
BC110596 mRNA. No translation available.
BC110597 mRNA. Translation: AAI10598.1. Different initiation.
RefSeqNP_851321.2. NM_180990.3.
UniGeneHs.714919.

3D structure databases

ProteinModelPortalQ401N2.
SMRQ401N2. Positions 52-323.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000334854.

Chemistry

ChEMBLCHEMBL1628479.
GuidetoPHARMACOLOGY587.

Polymorphism databases

DMDM166991030.

Proteomic databases

PaxDbQ401N2.
PRIDEQ401N2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000334586; ENSP00000334854; ENSG00000186919. [Q401N2-1]
ENST00000392503; ENSP00000376291; ENSG00000186919. [Q401N2-3]
ENST00000421794; ENSP00000391936; ENSG00000186919. [Q401N2-3]
ENST00000425015; ENSP00000397489; ENSG00000186919. [Q401N2-2]
GeneID353174.
KEGGhsa:353174.
UCSCuc002jqn.2. human. [Q401N2-1]

Organism-specific databases

CTD353174.
GeneCardsGC17P074075.
H-InvDBHIX0027141.
HGNCHGNC:29504. ZACN.
MIM610935. gene.
neXtProtNX_Q401N2.
PharmGKBPA162409377.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG121521.
HOGENOMHOG000155757.
HOVERGENHBG108756.
InParanoidQ401N2.
KOK16888.
OMALVPCFQV.
OrthoDBEOG7M98GM.
PhylomeDBQ401N2.
TreeFamTF315605.

Gene expression databases

BgeeQ401N2.
CleanExHS_ZACN.
GenevestigatorQ401N2.

Family and domain databases

Gene3D1.20.120.370. 1 hit.
2.70.170.10. 1 hit.
InterProIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
[Graphical view]
SUPFAMSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSZACN. human.
GeneWikiZinc-activated_ion_channel.
GenomeRNAi353174.
NextBio99731.
PROQ401N2.
SOURCESearch...

Entry information

Entry nameZACN_HUMAN
AccessionPrimary (citable) accession number: Q401N2
Secondary accession number(s): Q2TB29, Q6ZWK3, Q86YW4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 5, 2008
Last modified: April 16, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM