Q401N2 (ZACN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Zinc-activated ligand-gated ion channel

Alternative name(s):
Ligand-gated ion channel zinc-activated 1
Ligand-gated ion-channel receptor L2

Gene names

Name:	ZACN
Synonyms:	L2, LGICZ, LGICZ1, ZAC

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	412 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Zinc-activated ligand-gated ion channel. Ref.1
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.2.
Tissue specificity	Detected in pancreas, brain, liver, placenta, trachea, kidney, spinal cord, stomach and fetal brain. In the adult brain region expression is detected in the hippocampus, striatum, amygdala and thalamus. Ref.1 Ref.2
Post-translational modification	Glycosylated. Ref.2
Miscellaneous	The mouse and rat orthologous proteins do not exist.
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. [View classification]
Caution	It is uncertain whether Met-1 or Met-2 is the initiator.
Sequence caution	The sequence AAI10598.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAO20969.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence EAW89365.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Ligand	Zinc
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to zinc ion Inferred from direct assay Ref.1. Source: HGNC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.2. Source: HGNC postsynaptic membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	extracellular ligand-gated ion channel activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred by curator Ref.1. Source: HGNC
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q401N2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q401N2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     182-266: AMELEFQAHV...RAIERIGYKV → GADRAGAAGL...VLSGSLRAPL
     267-412: Missing.

Isoform 3 (identifier: Q401N2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     75-126: DILRYTMSSM...TPRLTILEAL → SSVLPRATAL...PPCINPSGDI
     127-412: Missing.

Note: No experimental confirmation available. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 412	387	Zinc-activated ligand-gated ion channel		PRO_0000317165
Regions
	Topological domain	26 – 233	208	Extracellular Potential
	Transmembrane	234 – 254	21	Helical; Name=1; Potential
	Topological domain	255 – 265	11	Cytoplasmic Potential
	Transmembrane	266 – 286	21	Helical; Name=2; Potential
	Topological domain	287 – 298	12	Extracellular Potential
	Transmembrane	299 – 319	21	Helical; Name=3; Potential
	Topological domain	320 – 368	49	Cytoplasmic Potential
	Transmembrane	369 – 389	21	Helical; Name=4; Potential
	Topological domain	390 – 412	23	Extracellular Potential
	Compositional bias	224 – 318	95	Leu-rich
Amino acid modifications
	Glycosylation	41	1	N-linked (GlcNAc...) Potential
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	170	1	N-linked (GlcNAc...) Potential		PRO_5000052473
	Disulfide bond	157 ↔ 171		By similarity
Natural variations
	Alternative sequence	75 – 126	52	DILRY…ILEAL → SSVLPRATALHSHGDCSGSA PCSKARGSGCHGLTPGNHLK VQPPCINPSGDI in isoform 3.		VSP_030909
	Alternative sequence	127 – 412	286	Missing in isoform 3.		VSP_030910
	Alternative sequence	182 – 266	85	AMELE…IGYKV → GADRAGAAGLRRGGGRWGRG TPRSVVQGQGAGQGEGAKAD RRRTPRSVFRAVYPRLRRAA PALPLRPPLEWQPISVLSGS LRAPL in isoform 2.		VSP_030911
	Alternative sequence	267 – 412	146	Missing in isoform 2.		VSP_030912
	Natural variant	152	1	A → T. Ref.1 Ref.3 Corresponds to variant rs2257020 [ dbSNP | Ensembl ].		VAR_038483

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 5, 2008. Version 2. Checksum: B894A28F5163723F	FASTA	412	45,816
	10 20 30 40 50 60 MMALWSLLHL TFLGFSITLL LVHGQGFQGT AAIWPSLFNV NLSKKVQESI QIPNNGSAPL 70 80 90 100 110 120 LVDVRVFVSN VFNVDILRYT MSSMLLLRLS WLDTRLAWNT SAHPRHAITL PWESLWTPRL 130 140 150 160 170 180 TILEALWVDW RDQSPQARVD QDGHVKLNLA LATETNCNFE LLHFPRDHSN CSLSFYALSN 190 200 210 220 230 240 TAMELEFQAH VVNEIVSVKR EYVVYDLKTQ VPPQQLVPCF QVTLRLKNTA LKSIIALLVP 250 260 270 280 290 300 AEALLLADVC GGLLPLRAIE RIGYKVTLLL SYLVLHSSLV QALPSSSSCN PLLIYYFTIL 310 320 330 340 350 360 LLLLFLSTIE TVLLAGLLAR GNLGAKSGPS PAPRGEQREH GNPGPHPAEE PSRGVKGSQR 370 380 390 400 410 SWPETADRIF FLVYVVGVLC TQFVFAGIWM WAACKSDAAP GEAAPHGRRP RL
	Isoform 2 [UniParc]. Checksum: 06C2C8684090C9C8	FASTA	266	29,616
	10 20 30 40 50 60 MMALWSLLHL TFLGFSITLL LVHGQGFQGT AAIWPSLFNV NLSKKVQESI QIPNNGSAPL 70 80 90 100 110 120 LVDVRVFVSN VFNVDILRYT MSSMLLLRLS WLDTRLAWNT SAHPRHAITL PWESLWTPRL 130 140 150 160 170 180 TILEALWVDW RDQSPQARVD QDGHVKLNLA LATETNCNFE LLHFPRDHSN CSLSFYALSN 190 200 210 220 230 240 TGADRAGAAG LRRGGGRWGR GTPRSVVQGQ GAGQGEGAKA DRRRTPRSVF RAVYPRLRRA 250 260 APALPLRPPL EWQPISVLSG SLRAPL
	Isoform 3 [UniParc]. Checksum: 9F87FF81BAB98B5F	FASTA	126	13,299
	10 20 30 40 50 60 MMALWSLLHL TFLGFSITLL LVHGQGFQGT AAIWPSLFNV NLSKKVQESI QIPNNGSAPL 70 80 90 100 110 120 LVDVRVFVSN VFNVSSVLPR ATALHSHGDC SGSAPCSKAR GSGCHGLTPG NHLKVQPPCI NPSGDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel class of ligand-gated ion channel is activated by Zn2+." Davies P.A., Wang W., Hales T.G., Kirkness E.F. J. Biol. Chem. 278:712-717(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, FUNCTION, VARIANT THR-152.
[2]	"Cloning and expression of ligand-gated ion-channel receptor L2 in central nervous system." Houtani T., Munemoto Y., Kase M., Sakuma S., Tsutsumi T., Sugimoto T. Biochem. Biophys. Res. Commun. 335:277-285(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION. Tissue: Brain.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANT THR-152. Tissue: Thymus.
[4]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF512521 mRNA. Translation: AAO20969.1. Different initiation. AB223030 mRNA. Translation: BAE19924.1. AK122638 mRNA. No translation available. AC018665 Genomic DNA. No translation available. CH471099 Genomic DNA. Translation: EAW89365.1. Different initiation. CH471099 Genomic DNA. Translation: EAW89366.1. BC110596 mRNA. No translation available. BC110597 mRNA. Translation: AAI10598.1. Different initiation.
IPI	IPI00329467. IPI00719125. IPI00885215.
RefSeq	NP_851321.2. NM_180990.3.
UniGene	Hs.714919.
3D structure databases
ProteinModelPortal	Q401N2.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000334854.
Polymorphism databases
DMDM	166991030.
Proteomic databases
PaxDb	Q401N2.
PRIDE	Q401N2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000334586; ENSP00000334854; ENSG00000186919. ENST00000392503; ENSP00000376291; ENSG00000186919. ENST00000421794; ENSP00000391936; ENSG00000186919. ENST00000425015; ENSP00000397489; ENSG00000186919.
GeneID	353174.
KEGG	hsa:353174.
UCSC	uc002jqn.2. human.
Organism-specific databases
CTD	353174.
GeneCards	GC17P074075.
H-InvDB	HIX0027141.
HGNC	HGNC:29504. ZACN.
MIM	610935. gene.
neXtProt	NX_Q401N2.
PharmGKB	PA162409377.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG121521.
HOGENOM	HOG000155757.
HOVERGEN	HBG108756.
InParanoid	Q401N2.
OMA	LVPCFQV.
OrthoDB	EOG4Z8XWW.
Gene expression databases
Bgee	Q401N2.
CleanEx	HS_ZACN.
Genevestigator	Q401N2.
Family and domain databases
Gene3D	2.70.170.10. 1 hit.
InterPro	IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. [Graphical view]
PANTHER	PTHR18945. PTHR18945. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. [Graphical view]
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1628479.
ChiTaRS	ZACN. human.
GenomeRNAi	353174.
NextBio	99731.
SOURCE	Search...

Entry information

Entry name

ZACN_HUMAN

Accession

Primary (citable) accession number: Q401N2
Secondary accession number(s): Q2TB29, Q6ZWK3, Q86YW4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 5, 2008
Last modified:	May 1, 2013
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.