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Protein

Polygalacturonase-1 non-catalytic subunit beta

Gene

GP1

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Non-catalytic subunit of the polygalacturonase isozyme 1 (PG1). Necessary and sufficient to convert the polygalacturonase from its monomeric form PG2 to its heterodimeric form PG1. Seems to limit the depolymerization and solubilization of cell wall polyuronides mediated by PG2 during ripening, probably by recruiting PG2 to form PG1.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell wall biogenesis/degradation, Fruit ripening

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase-1 non-catalytic subunit beta
Alternative name(s):
AroGP1
Polygalacturonase converter
Short name:
PG converter
Gene namesi
Name:GP1
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
ProteomesiUP000004994 Componenti: Chromosome 5

Subcellular locationi

  • Secretedextracellular spaceapoplast 1 Publication
  • Secretedcell wall 1 Publication

  • Note: Associated to the cell wall.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Propeptidei28 – 108811 PublicationPRO_0000042959Add
BLAST
Chaini109 – ?397289Polygalacturonase-1 non-catalytic subunit betaPRO_0000042960Add
BLAST
Propeptidei?398 – 630233PRO_0000042961Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi334 – 3341N-linked (GlcNAc...)Sequence Analysis
Glycosylationi369 – 3691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Mostly expressed in fruit pericarp. Also detected at low levels in cell wall of roots, leaves and flowers (at protein level).5 Publications

Developmental stagei

Expressed in ripening fruits from the 20th day after anthesis and increase during the ripening (at protein level).4 Publications

Inductioni

Transiently repressed by ethylene.1 Publication

Interactioni

Subunit structurei

Interacts with polygalacturonase-2 (isoenzymes PG2A and PG2B) to form heterodimers called polygalacturonase-1 (PG1).4 Publications

Protein-protein interaction databases

STRINGi4081.Solyc05g005560.2.1.

Structurei

3D structure databases

ProteinModelPortaliQ40161.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini415 – 629215BURPPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 BURP domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiQ40161.
OMAiHIGFKEY.

Family and domain databases

InterProiIPR004873. BURP_dom.
[Graphical view]
PfamiPF03181. BURP. 1 hit.
[Graphical view]
PROSITEiPS51277. BURP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q40161-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHTKIHLPPC ILLLLLFSLP SFNVVVGGDG ESGNPFTPKG YLIRYWKKQI
60 70 80 90 100
SNDLPKPWFL LNKASPLNAA QYATYTKLVA DQNALTTQLH TFCSSANLMC
110 120 130 140 150
APDLSPSLEK HSGDIHFATY SDKNFTNYGT NEPGIGVNTF KNYSEGENIP
160 170 180 190 200
VNSFRRYGRG SPRDNKFDNY ASDGNVIDQS FNSYSTSTAG GSGKFTNYAA
210 220 230 240 250
NANDPNLHFT SYSDQGTGGV QKFTIYSQEA NAGDQYFKSY GKNGNGANGE
260 270 280 290 300
FVSYGNDTNV IGSTFTNYGQ TANGGDQKFT SYGFNGNVPE NHFTNYGAGG
310 320 330 340 350
NGPSETFNSY RDQSNVGDDT FTTYVKDANG GEANFTNYGQ SFNEGTDVFT
360 370 380 390 400
TYGKGGNDPH INFKTYGVNN TFKDYVKDTA TFSNYHNKTS QVLASLMEVN
410 420 430 440 450
GGKKVNNRWV EPGKFFREKM LKSGTIMPMP DIKDKMPKRS FLPRVIASKL
460 470 480 490 500
PFSTSKIAEL KKIFHAGDES QVEKMIGDAL SECERAPSAG ETKRCVNSAE
510 520 530 540 550
DMIDFATSVL GRNVVVRTTE DTKGSNGNIM IGSVKGINGG KVTKSVSCHQ
560 570 580 590 600
TLYPYLLYYC HSVPKVRVYE ADILDPNSKV KINHGVAICH VDTSSWGPSH
610 620 630
GAFVALGSGP GKIEVCHWIF ENDMTWAIAD
Length:630
Mass (Da):68,960
Last modified:November 1, 1996 - v1
Checksum:i31D585305D216AFE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti250 – 2501E → Q AA sequence (PubMed:1392611).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98466 mRNA. Translation: AAA34181.1.
U63374 Genomic DNA. Translation: AAB39547.1.
PIRiJQ1670.
RefSeqiNP_001234835.1. NM_001247906.1.
UniGeneiLes.12828.

Genome annotation databases

EnsemblPlantsiSolyc05g005560.2.1; Solyc05g005560.2.1; Solyc05g005560.2.
GeneIDi543991.
KEGGisly:543991.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M98466 mRNA. Translation: AAA34181.1.
U63374 Genomic DNA. Translation: AAB39547.1.
PIRiJQ1670.
RefSeqiNP_001234835.1. NM_001247906.1.
UniGeneiLes.12828.

3D structure databases

ProteinModelPortaliQ40161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4081.Solyc05g005560.2.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiSolyc05g005560.2.1; Solyc05g005560.2.1; Solyc05g005560.2.
GeneIDi543991.
KEGGisly:543991.

Phylogenomic databases

InParanoidiQ40161.
OMAiHIGFKEY.

Family and domain databases

InterProiIPR004873. BURP_dom.
[Graphical view]
PfamiPF03181. BURP. 1 hit.
[Graphical view]
PROSITEiPS51277. BURP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The beta subunit of tomato fruit polygalacturonase isoenzyme 1: isolation, characterization, and identification of unique structural features."
    Zheng L., Heupel R.C., DellaPenna D.
    Plant Cell 4:1147-1156(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 109-120; 160-171; 230-236 AND 243-252, PROPEPTIDES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH PG POLYPEPTIDES.
    Strain: cv. Ailsa Craig.
    Tissue: Fruit.
  2. "Gene encoding tomato polygalacturonase 1 (PG1) beta subunit."
    Watson C.F., Schuchman B., Liu J., DellaPenna D.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. VFNT Cherry.
  3. "Purification and characterization of tomato polygalacturonase converter."
    Pressey R.
    Eur. J. Biochem. 144:217-221(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PG2, TISSUE SPECIFICITY.
  4. "Accumulation of the beta-subunit of polygalacturonase 1 in normal and mutant tomato fruit."
    Pogson B.J., Brady C.J.
    Planta 191:71-78(1993)
    Cited for: INTERACTION WITH PG2, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  5. "Reduction of tomato polygalacturonase beta subunit expression affects pectin solubilization and degradation during fruit ripening."
    Watson C.F., Zheng L., DellaPenna D.
    Plant Cell 6:1623-1634(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE.
  6. "Differential expression of the two subunits of tomato polygalacturonase isoenzyme 1 in wild-type and in tomato fruit."
    Zheng L., Watson C.F., DellaPenna D.
    Plant Physiol. 105:1189-1195(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
  7. "Tomato fruit polygalacturonase isozyme 1 -- characterization of the beta subunit and its state of assembly in vivo."
    Moore T., Bennett A.B.
    Plant Physiol. 106:1461-1469(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, TISSUE SPECIFICITY.

Entry informationi

Entry nameiGP1_SOLLC
AccessioniPrimary (citable) accession number: Q40161
Secondary accession number(s): O04735
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: June 24, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.