Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q40161 (GP1_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Polygalacturonase-1 non-catalytic subunit beta
Alternative name(s):
AroGP1
Polygalacturonase converter
Short name=PG converter
Gene names
Name:GP1
OrganismSolanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier4081 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon

Protein attributes

Sequence length630 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Non-catalytic subunit of the polygalacturonase isozyme 1 (PG1). Necessary and sufficient to convert the polygalacturonase from its monomeric form PG2 to its heterodimeric form PG1. Seems to limit the depolymerization and solubilization of cell wall polyuronides mediated by PG2 during ripening, probably by recruiting PG2 to form PG1. Ref.5 Ref.7

Subunit structure

Interacts with polygalacturonase-2 (isoenzymes PG2A and PG2B) to form heterodimers called polygalacturonase-1 (PG1). Ref.1 Ref.3 Ref.4 Ref.7

Subcellular location

Secretedextracellular spaceapoplast. Secretedcell wall. Note: Associated to the cell wall. Ref.6

Tissue specificity

Mostly expressed in fruit pericarp. Also detected at low levels in cell wall of roots, leaves and flowers (at protein level). Ref.1 Ref.3 Ref.4 Ref.6 Ref.7

Developmental stage

Expressed in ripening fruits from the 20th day after anthesis and increase during the ripening (at protein level). Ref.1 Ref.4 Ref.5 Ref.6

Induction

Transiently repressed by ethylene. Ref.6

Sequence similarities

Contains 1 BURP domain.

Ontologies

Keywords
   Biological processCell wall biogenesis/degradation
Fruit ripening
   Cellular componentApoplast
Cell wall
Secreted
   DomainSignal
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processfruit ripening

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentapoplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

cell wall

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Propeptide28 – 10881
PRO_0000042959
Chain109 – ?397289Polygalacturonase-1 non-catalytic subunit beta
PRO_0000042960
Propeptide?398 – 630233
PRO_0000042961

Regions

Domain415 – 629215BURP

Amino acid modifications

Glycosylation1241N-linked (GlcNAc...) Potential
Glycosylation1421N-linked (GlcNAc...) Potential
Glycosylation2561N-linked (GlcNAc...) Potential
Glycosylation3341N-linked (GlcNAc...) Potential
Glycosylation3691N-linked (GlcNAc...) Potential
Glycosylation3871N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict2501E → Q AA sequence Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q40161 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 31D585305D216AFE

FASTA63068,960
        10         20         30         40         50         60 
MHTKIHLPPC ILLLLLFSLP SFNVVVGGDG ESGNPFTPKG YLIRYWKKQI SNDLPKPWFL 

        70         80         90        100        110        120 
LNKASPLNAA QYATYTKLVA DQNALTTQLH TFCSSANLMC APDLSPSLEK HSGDIHFATY 

       130        140        150        160        170        180 
SDKNFTNYGT NEPGIGVNTF KNYSEGENIP VNSFRRYGRG SPRDNKFDNY ASDGNVIDQS 

       190        200        210        220        230        240 
FNSYSTSTAG GSGKFTNYAA NANDPNLHFT SYSDQGTGGV QKFTIYSQEA NAGDQYFKSY 

       250        260        270        280        290        300 
GKNGNGANGE FVSYGNDTNV IGSTFTNYGQ TANGGDQKFT SYGFNGNVPE NHFTNYGAGG 

       310        320        330        340        350        360 
NGPSETFNSY RDQSNVGDDT FTTYVKDANG GEANFTNYGQ SFNEGTDVFT TYGKGGNDPH 

       370        380        390        400        410        420 
INFKTYGVNN TFKDYVKDTA TFSNYHNKTS QVLASLMEVN GGKKVNNRWV EPGKFFREKM 

       430        440        450        460        470        480 
LKSGTIMPMP DIKDKMPKRS FLPRVIASKL PFSTSKIAEL KKIFHAGDES QVEKMIGDAL 

       490        500        510        520        530        540 
SECERAPSAG ETKRCVNSAE DMIDFATSVL GRNVVVRTTE DTKGSNGNIM IGSVKGINGG 

       550        560        570        580        590        600 
KVTKSVSCHQ TLYPYLLYYC HSVPKVRVYE ADILDPNSKV KINHGVAICH VDTSSWGPSH 

       610        620        630 
GAFVALGSGP GKIEVCHWIF ENDMTWAIAD 

« Hide

References

[1]"The beta subunit of tomato fruit polygalacturonase isoenzyme 1: isolation, characterization, and identification of unique structural features."
Zheng L., Heupel R.C., DellaPenna D.
Plant Cell 4:1147-1156(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 109-120; 160-171; 230-236 AND 243-252, PROPEPTIDES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH PG POLYPEPTIDES.
Strain: cv. Ailsa Craig.
Tissue: Fruit.
[2]"Gene encoding tomato polygalacturonase 1 (PG1) beta subunit."
Watson C.F., Schuchman B., Liu J., DellaPenna D.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. VFNT Cherry.
[3]"Purification and characterization of tomato polygalacturonase converter."
Pressey R.
Eur. J. Biochem. 144:217-221(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PG2, TISSUE SPECIFICITY.
[4]"Accumulation of the beta-subunit of polygalacturonase 1 in normal and mutant tomato fruit."
Pogson B.J., Brady C.J.
Planta 191:71-78(1993)
Cited for: INTERACTION WITH PG2, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[5]"Reduction of tomato polygalacturonase beta subunit expression affects pectin solubilization and degradation during fruit ripening."
Watson C.F., Zheng L., DellaPenna D.
Plant Cell 6:1623-1634(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE.
[6]"Differential expression of the two subunits of tomato polygalacturonase isoenzyme 1 in wild-type and in tomato fruit."
Zheng L., Watson C.F., DellaPenna D.
Plant Physiol. 105:1189-1195(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
[7]"Tomato fruit polygalacturonase isozyme 1 -- characterization of the beta subunit and its state of assembly in vivo."
Moore T., Bennett A.B.
Plant Physiol. 106:1461-1469(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M98466 mRNA. Translation: AAA34181.1.
U63374 Genomic DNA. Translation: AAB39547.1.
PIRJQ1670.
RefSeqNP_001234835.1. NM_001247906.1.
UniGeneLes.12828.

3D structure databases

ProteinModelPortalQ40161.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsSolyc05g005560.2.1; Solyc05g005560.2.1; Solyc05g005560.2.
GeneID543991.
KEGGsly:543991.

Phylogenomic databases

OMAYSQEANH.

Family and domain databases

InterProIPR004873. BURP_dom.
[Graphical view]
PfamPF03181. BURP. 1 hit.
[Graphical view]
PROSITEPS51277. BURP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGP1_SOLLC
AccessionPrimary (citable) accession number: Q40161
Secondary accession number(s): O04735
Entry history
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1996
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families