Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q40161

- GP1_SOLLC

UniProt

Q40161 - GP1_SOLLC

Protein

Polygalacturonase-1 non-catalytic subunit beta

Gene

GP1

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Non-catalytic subunit of the polygalacturonase isozyme 1 (PG1). Necessary and sufficient to convert the polygalacturonase from its monomeric form PG2 to its heterodimeric form PG1. Seems to limit the depolymerization and solubilization of cell wall polyuronides mediated by PG2 during ripening, probably by recruiting PG2 to form PG1.2 Publications

    GO - Biological processi

    1. fruit ripening Source: UniProtKB-KW

    Keywords - Biological processi

    Cell wall biogenesis/degradation, Fruit ripening

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polygalacturonase-1 non-catalytic subunit beta
    Alternative name(s):
    AroGP1
    Polygalacturonase converter
    Short name:
    PG converter
    Gene namesi
    Name:GP1
    OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
    Taxonomic identifieri4081 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
    ProteomesiUP000004994: Chromosome 5

    Subcellular locationi

    Secretedextracellular spaceapoplast 1 Publication. Secretedcell wall 1 Publication
    Note: Associated to the cell wall.

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Propeptidei28 – 108811 PublicationPRO_0000042959Add
    BLAST
    Chaini109 – ?397289Polygalacturonase-1 non-catalytic subunit betaPRO_0000042960Add
    BLAST
    Propeptidei?398 – 630233PRO_0000042961Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi124 – 1241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi256 – 2561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi334 – 3341N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi369 – 3691N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Mostly expressed in fruit pericarp. Also detected at low levels in cell wall of roots, leaves and flowers (at protein level).5 Publications

    Developmental stagei

    Expressed in ripening fruits from the 20th day after anthesis and increase during the ripening (at protein level).4 Publications

    Inductioni

    Transiently repressed by ethylene.1 Publication

    Interactioni

    Subunit structurei

    Interacts with polygalacturonase-2 (isoenzymes PG2A and PG2B) to form heterodimers called polygalacturonase-1 (PG1).4 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliQ40161.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini415 – 629215BURPPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 BURP domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    OMAiYSQEANH.

    Family and domain databases

    InterProiIPR004873. BURP_dom.
    [Graphical view]
    PfamiPF03181. BURP. 1 hit.
    [Graphical view]
    PROSITEiPS51277. BURP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q40161-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHTKIHLPPC ILLLLLFSLP SFNVVVGGDG ESGNPFTPKG YLIRYWKKQI    50
    SNDLPKPWFL LNKASPLNAA QYATYTKLVA DQNALTTQLH TFCSSANLMC 100
    APDLSPSLEK HSGDIHFATY SDKNFTNYGT NEPGIGVNTF KNYSEGENIP 150
    VNSFRRYGRG SPRDNKFDNY ASDGNVIDQS FNSYSTSTAG GSGKFTNYAA 200
    NANDPNLHFT SYSDQGTGGV QKFTIYSQEA NAGDQYFKSY GKNGNGANGE 250
    FVSYGNDTNV IGSTFTNYGQ TANGGDQKFT SYGFNGNVPE NHFTNYGAGG 300
    NGPSETFNSY RDQSNVGDDT FTTYVKDANG GEANFTNYGQ SFNEGTDVFT 350
    TYGKGGNDPH INFKTYGVNN TFKDYVKDTA TFSNYHNKTS QVLASLMEVN 400
    GGKKVNNRWV EPGKFFREKM LKSGTIMPMP DIKDKMPKRS FLPRVIASKL 450
    PFSTSKIAEL KKIFHAGDES QVEKMIGDAL SECERAPSAG ETKRCVNSAE 500
    DMIDFATSVL GRNVVVRTTE DTKGSNGNIM IGSVKGINGG KVTKSVSCHQ 550
    TLYPYLLYYC HSVPKVRVYE ADILDPNSKV KINHGVAICH VDTSSWGPSH 600
    GAFVALGSGP GKIEVCHWIF ENDMTWAIAD 630
    Length:630
    Mass (Da):68,960
    Last modified:November 1, 1996 - v1
    Checksum:i31D585305D216AFE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti250 – 2501E → Q AA sequence (PubMed:1392611)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98466 mRNA. Translation: AAA34181.1.
    U63374 Genomic DNA. Translation: AAB39547.1.
    PIRiJQ1670.
    RefSeqiNP_001234835.1. NM_001247906.1.
    UniGeneiLes.12828.

    Genome annotation databases

    EnsemblPlantsiSolyc05g005560.2.1; Solyc05g005560.2.1; Solyc05g005560.2.
    GeneIDi543991.
    KEGGisly:543991.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M98466 mRNA. Translation: AAA34181.1 .
    U63374 Genomic DNA. Translation: AAB39547.1 .
    PIRi JQ1670.
    RefSeqi NP_001234835.1. NM_001247906.1.
    UniGenei Les.12828.

    3D structure databases

    ProteinModelPortali Q40161.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi Solyc05g005560.2.1 ; Solyc05g005560.2.1 ; Solyc05g005560.2 .
    GeneIDi 543991.
    KEGGi sly:543991.

    Phylogenomic databases

    OMAi YSQEANH.

    Family and domain databases

    InterProi IPR004873. BURP_dom.
    [Graphical view ]
    Pfami PF03181. BURP. 1 hit.
    [Graphical view ]
    PROSITEi PS51277. BURP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The beta subunit of tomato fruit polygalacturonase isoenzyme 1: isolation, characterization, and identification of unique structural features."
      Zheng L., Heupel R.C., DellaPenna D.
      Plant Cell 4:1147-1156(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 109-120; 160-171; 230-236 AND 243-252, PROPEPTIDES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH PG POLYPEPTIDES.
      Strain: cv. Ailsa Craig.
      Tissue: Fruit.
    2. "Gene encoding tomato polygalacturonase 1 (PG1) beta subunit."
      Watson C.F., Schuchman B., Liu J., DellaPenna D.
      Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. VFNT Cherry.
    3. "Purification and characterization of tomato polygalacturonase converter."
      Pressey R.
      Eur. J. Biochem. 144:217-221(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PG2, TISSUE SPECIFICITY.
    4. "Accumulation of the beta-subunit of polygalacturonase 1 in normal and mutant tomato fruit."
      Pogson B.J., Brady C.J.
      Planta 191:71-78(1993)
      Cited for: INTERACTION WITH PG2, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    5. "Reduction of tomato polygalacturonase beta subunit expression affects pectin solubilization and degradation during fruit ripening."
      Watson C.F., Zheng L., DellaPenna D.
      Plant Cell 6:1623-1634(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE.
    6. "Differential expression of the two subunits of tomato polygalacturonase isoenzyme 1 in wild-type and in tomato fruit."
      Zheng L., Watson C.F., DellaPenna D.
      Plant Physiol. 105:1189-1195(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
    7. "Tomato fruit polygalacturonase isozyme 1 -- characterization of the beta subunit and its state of assembly in vivo."
      Moore T., Bennett A.B.
      Plant Physiol. 106:1461-1469(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiGP1_SOLLC
    AccessioniPrimary (citable) accession number: Q40161
    Secondary accession number(s): O04735
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3