Q40147 (GSA_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic Short name=GSA EC=5.4.3.8 Alternative name(s): Glutamate-1-semialdehyde aminotransferase Short name=GSA-AT
Organism	Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [Reference proteome]
Taxonomic identifier	4081 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	(S)-4-amino-5-oxopentanoate = 5-aminolevulinate.
Cofactor	Pyridoxal phosphate.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 2/2. Porphyrin biosynthesis; chlorophyll biosynthesis.
Subunit structure	Homodimer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway protoporphyrinogen IX biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glutamate-1-semialdehyde 2,1-aminomutase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transaminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast
	Chain	? – 481		Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic		PRO_0000001259
Amino acid modifications
	Modified residue	321	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q40147 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 4D7EFC0E32D64BBD	FASTA	481	51,413
	10 20 30 40 50 60 MAAVNGVGLS WPSKLTKNQT PKWGFSPSHR RCNPSSSSSA TIRMTASVDE KKKTFTLEKS 70 80 90 100 110 120 EEAFSKAKEL MPGGVNSPVR AFKSVGGQPI IIDSVKGSRM RDIDGNEYID YVGSWGPAII 130 140 150 160 170 180 GHADDEVLAA LAETMKKGTS FGAPCLLENT LAEMVISAVP SIEMVRFVNS GTEACMGVLR 190 200 210 220 230 240 LARAFTCRPK IIKFEGCYHG HADPFLVKAG SGVATLGLPD SPGVPKAATI DTLTAPYNDI 250 260 270 280 290 300 SAVESLFEEH KGEIAAVILE PVVGNAGFIP PKLEFLAAIR KITKENDALL IFDEVMTGFR 310 320 330 340 350 360 LAYGGAQEYF GITPDLTTLG KIIGGGLPVG AYGGRRDIME MVAPAGPMYQ AGTLSGNPLA 370 380 390 400 410 420 MTAGIHTLKR LQGQGTYEHL DKITAELTQG ILDAGKKTGH AMCGGSIRGM FGFFFADGPI 430 440 450 460 470 480 YNFSDAKKSD TEKFGRFYRG MLEEGVYFAP SQFEAGFTSL AHTPEDIQRT VAAAEKVLKQ I

References

[1]	"A cDNA clone for glutamate 1-semialdehyde 2,1-aminomutase from tomato (Lycopersicon esculentum Mill.)." Polking G.F., Hannapel D.J., Gladon R.J. Plant Gene Register PGR95-035 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. VFNT Cherry. Tissue: Fruit.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L39279 mRNA. Translation: AAA81881.1.
PIR	T07034.
RefSeq	NP_001234690.1. NM_001247761.1.
UniGene	Les.4018.
3D structure databases
ProteinModelPortal	Q40147.
SMR	Q40147. Positions 55-481.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	543967.
Enzyme and pathway databases
UniPathway	UPA00251; UER00317. UPA00668.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 2 hits.
InterPro	IPR004639. 4pyrrol_synth_GluAld_NH2Trfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
PANTHER	PTHR11986. PTHR11986. 1 hit. PTHR11986:SF5. PTHR11986:SF5. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00713. hemL. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSA_SOLLC

Accession

Primary (citable) accession number: Q40147

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	March 6, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program