ID   CHIA_SOLCI              Reviewed;         253 AA.
AC   Q40114; Q40113;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   16-JUN-2009, entry version 56.
DE   RecName: Full=Acidic endochitinase pcht28;
DE            EC=3.2.1.14;
DE   Flags: Precursor;
OS   Solanum chilense (Tomato) (Lycopersicon chilense).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae;
OC   Solanum; Lycopersicon.
OX   NCBI_TaxID=4083;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. LA1930; TISSUE=Leaf;
RX   MEDLINE=95115667; PubMed=7816027; DOI=10.1007/BF00283267;
RA   Chen R.D., Yu L.X., Greer A.F., Cheriti H., Tabaeizadeh Z.;
RT   "Isolation of an osmotic stress- and abscisic acid-induced gene
RT   encoding an acidic endochitinase from Lycopersicon chilense.";
RL   Mol. Gen. Genet. 245:195-202(1994).
CC   -!- FUNCTION: Defense against chitin containing fungal pathogens.
CC   -!- CATALYTIC ACTIVITY: Random hydrolysis of N-acetyl-beta-D-
CC       glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space.
CC   -!- INDUCTION: By osmotic stress and abscisic acid (ABA).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 19 family. Chitinase
CC       class II subfamily.
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DR   EMBL; L19342; AAA64999.1; -; mRNA.
DR   EMBL; M97210; AAA64998.1; -; mRNA.
DR   PIR; S51588; S51588.
DR   PIR; S51589; S51589.
DR   HSSP; P23951; 2BAA.
DR   CAZy; GH19; Glycoside Hydrolase Family 19.
DR   BRENDA; 3.2.1.14; 292969.
DR   GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR   GO; GO:0004568; F:chitinase activity; IEA:EC.
DR   GO; GO:0016998; P:cell wall macromolecule catabolic process; IEA:InterPro.
DR   GO; GO:0006032; P:chitin catabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR016283; Glyco_hydro_19.
DR   InterPro; IPR000726; Glyco_hydro_19_cat.
DR   PANTHER; PTHR22595; Glyco_hydro_19_cat; 1.
DR   Pfam; PF00182; Glyco_hydro_19; 1.
DR   PIRSF; PIRSF001060; Endochitinase; 1.
DR   ProDom; PD354900; Glyco_hydro_19; 1.
DR   PROSITE; PS00773; CHITINASE_19_1; 1.
DR   PROSITE; PS00774; CHITINASE_19_2; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Chitin degradation; Disulfide bond;
KW   Glycosidase; Hydrolase; Plant defense; Polysaccharide degradation;
KW   Secreted; Signal; Stress response.
FT   SIGNAL        1     24       By similarity.
FT   CHAIN        25    253       Acidic endochitinase pcht28.
FT                                /FTId=PRO_0000005298.
FT   DISULFID    212    244       By similarity.
FT   CONFLICT     94     94       K -> N (in Ref. 1; AAA64998).
FT   CONFLICT    160    160       R -> K (in Ref. 1; AAA64998).
FT   CONFLICT    170    171       QD -> HH (in Ref. 1; AAA64998).
SQ   SEQUENCE   253 AA;  27569 MW;  D48C269D5A794E60 CRC64;
     MKFNIVSPVA LSCLFFLFLT GTLAQNAGSI VTRELFEQML SFRNNDACPA KGFYTYDAFI
     AAANSFPGFG TTGDDTARKK EIAAFFGQTS HETKGGSAGT FTGGYCFVRQ IDQSDRYYGR
     GPIQLTHQSN YERAGQGIGV GQDLVNNPDL VATDPIISFR TAIWFWMTAQ DNKPSCHNVI
     IGQWTPSPAD TAANRVPGYG VITNIINGGL ECNMGPNTAV ESRIGFYRRY CGMLNVPTGE
     NLDCNNQKNF AQG
//