Reviewed, UniProtKB/Swiss-Prot Q40114 (CHIA_SOLCI)

Names and origin

Protein names	Recommended name:     Acidic endochitinase pcht28     EC=3.2.1.14
Organism	Solanum chilense (Tomato) (Lycopersicon chilense)
Taxonomic identifier	4083 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Subcellular location	Secreted › extracellular space.
Induction	By osmotic stress and abscisic acid (ABA).
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class II subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation Stress response
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW polysaccharide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	By similarity
	Chain	25 – 253	229	Acidic endochitinase pcht28		PRO_0000005298
Amino acid modifications
	Disulfide bond	212 ↔ 244		By similarity
Experimental info
	Sequence conflict	94	1	K → N in AAA64998. Ref.1
	Sequence conflict	160	1	R → K in AAA64998. Ref.1
	Sequence conflict	170 – 171	2	QD → HH in AAA64998. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q40114-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: D48C269D5A794E60	FASTA	253	27,569
	10 20 30 40 50 60 MKFNIVSPVA LSCLFFLFLT GTLAQNAGSI VTRELFEQML SFRNNDACPA KGFYTYDAFI 70 80 90 100 110 120 AAANSFPGFG TTGDDTARKK EIAAFFGQTS HETKGGSAGT FTGGYCFVRQ IDQSDRYYGR 130 140 150 160 170 180 GPIQLTHQSN YERAGQGIGV GQDLVNNPDL VATDPIISFR TAIWFWMTAQ DNKPSCHNVI 190 200 210 220 230 240 IGQWTPSPAD TAANRVPGYG VITNIINGGL ECNMGPNTAV ESRIGFYRRY CGMLNVPTGE 250 NLDCNNQKNF AQG

References

[1]

"Isolation of an osmotic stress- and abscisic acid-induced gene encoding an acidic endochitinase from Lycopersicon chilense." Chen R.D., Yu L.X., Greer A.F., Cheriti H., Tabaeizadeh Z. Mol. Gen. Genet. 245:195-202(1994) [PubMed: 7816027] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. LA1930. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L19342 mRNA. Translation: AAA64999.1. M97210 mRNA. Translation: AAA64998.1.
PIR	S51588. S51589.
3D structure databases
HSSP	HSSP built from PDB template 2BAA based on UniProtKB P23951.
ModBase	Search...
Protein family/group databases
CAZy	GH19. Glycoside Hydrolase Family 19.
Enzyme and pathway databases
BRENDA	3.2.1.14. 292969.
Family and domain databases
InterPro	IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
ProDom	PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIA_SOLCI

Accession

Primary (citable) accession number: Q40114
Secondary accession number(s): Q40113

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)