ID   MHPB1_PSEPU             Reviewed;         314 AA.
AC   Q400K4;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 1.
DT   16-JUN-2009, entry version 17.
DE   RecName: Full=2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase 1;
DE            EC=1.13.11.16;
GN   Name=mhpB1; Synonyms=orcB;
OS   Pseudomonas putida.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ORC;
RA   Straganz G.D., Glieder A., Steiner W.;
RT   "cloning of genes involved in meta-cleavage pathways of Pseudomonas
RT   putida orc: nucleotide sequences of genes, characterization of two
RT   dioxygenases and identification of the orcinol pathway.";
RL   Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the non-heme iron(II)-dependent oxidative
CC       cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-
CC       dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-
CC       hydroxy-6-ketononatrienedioate, respectively (By similarity).
CC   -!- CATALYTIC ACTIVITY: 3-(2,3-dihydroxyphenyl)propanoate + O(2) = 2-
CC       hydroxy-6-oxonona-2,4-diene-1,9-dioate.
CC   -!- CATALYTIC ACTIVITY: 2,3-dihydroxicinnamic acid + O(2) = 2-hydroxy-
CC       6-oxonona-2,4,7-triene-1,9-dioate.
CC   -!- COFACTOR: Fe(2+) ion (By similarity).
CC   -!- PATHWAY: Aromatic compound metabolism; 3-phenylpropionic acid
CC       degradation.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the ligB/mhpB extradiol dioxygenase family.
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DR   EMBL; AF534914; AAQ10534.1; -; Genomic_DNA.
DR   GO; GO:0008669; F:2,3-dihydroxy-phenylpropionate 1,2-dioxygen...; IEA:HAMAP.
DR   GO; GO:0047070; F:3-carboxyethylcatechol 2,3-dioxygenase acti...; IEA:EC.
DR   GO; GO:0008198; F:ferrous iron binding; IEA:InterPro.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01653; -; 1.
DR   InterPro; IPR004183; Xdiol_dOase_3B.
DR   Pfam; PF02900; LigB; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; Dioxygenase; Iron; Oxidoreductase.
FT   CHAIN         1    314       2,3-dihydroxyphenylpropionate/2,3-
FT                                dihydroxicinnamic acid 1,2-dioxygenase 1.
FT                                /FTId=PRO_0000337662.
FT   ACT_SITE    115    115       Proton donor (By similarity).
FT   ACT_SITE    179    179       Proton acceptor (By similarity).
SQ   SEQUENCE   314 AA;  33843 MW;  8EA8A7FB34F13D0F CRC64;
     MSAYLHCLSH TPLVGYVDPV AEVLAEVDEV VAAARARIAA FDPQLVFLFA PDHYNGFFYD
     VMPSFCIGMA ATAIGDFHSL AGPLDVPRET AEACAAAVLE AGVDAAVSYR MQVDHGFAQP
     LELLLGGLAE KPVVPVFING VAVPLPGFQR ARLLGEAIGR FARSTGKRVL FLGSGGLSHQ
     PPVPELAKVD ARMADRLLGS GRDLPTDERQ ARQQRVISAA EGFVADQNSL HPLNPEWDNH
     FLDLLEQRRF AELDGLGNAE LSALAGKSTH EVKTWVTAFA ALSAFGPYQA HERYYRPIPE
     WIAGFGSLSA HSLS
//