Reviewed, UniProtKB/Swiss-Prot Q400K4 (MHPB1_PSEPU)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase 1
    EC=1.13.11.16

Gene names

Name:	mhpB1
Synonyms:	orcB

Organism

Pseudomonas putida

Taxonomic identifier

303 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity.
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O(2) = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. 2,3-dihydroxicinnamic acid + O(2) = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate.
Cofactor	Fe(2+) ion By similarity.
Pathway	Aromatic compound metabolism; 3-phenylpropionic acid degradation.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP 3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase 1		PRO_0000337662
Sites
	Active site	115	1	Proton donor By similarity
	Active site	179	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q400K4-1 [UniParc]. Last modified September 27, 2005. Version 1. Checksum: 8EA8A7FB34F13D0F	FASTA	314	33,843
	10 20 30 40 50 60 MSAYLHCLSH TPLVGYVDPV AEVLAEVDEV VAAARARIAA FDPQLVFLFA PDHYNGFFYD 70 80 90 100 110 120 VMPSFCIGMA ATAIGDFHSL AGPLDVPRET AEACAAAVLE AGVDAAVSYR MQVDHGFAQP 130 140 150 160 170 180 LELLLGGLAE KPVVPVFING VAVPLPGFQR ARLLGEAIGR FARSTGKRVL FLGSGGLSHQ 190 200 210 220 230 240 PPVPELAKVD ARMADRLLGS GRDLPTDERQ ARQQRVISAA EGFVADQNSL HPLNPEWDNH 250 260 270 280 290 300 FLDLLEQRRF AELDGLGNAE LSALAGKSTH EVKTWVTAFA ALSAFGPYQA HERYYRPIPE 310 WIAGFGSLSA HSLS

References

[1]

"cloning of genes involved in meta-cleavage pathways of Pseudomonas putida orc: nucleotide sequences of genes, characterization of two dioxygenases and identification of the orcinol pathway." Straganz G.D., Glieder A., Steiner W. Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ORC.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF534914 Genomic DNA. Translation: AAQ10534.1.
3D structure databases
ModBase	Search...
Family and domain databases
HAMAP	MF_01653. [Tree]
InterPro	IPR004183. Xdiol_dOase_3B. [Graphical view]
Gene3D	G3DSA:3.40.830.10. Xdiol_dOase_3B. 1 hit.
Pfam	PF02900. LigB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPB1_PSEPU

Accession

Primary (citable) accession number: Q400K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	September 27, 2005
Last modified:	November 25, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)